Amino acid dipepetide frequency for Flavobacterium sp. FPG59

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.659AlaAla: 4.659 ± 0.102
0.565AlaCys: 0.565 ± 0.024
3.292AlaAsp: 3.292 ± 0.078
3.785AlaGlu: 3.785 ± 0.087
3.423AlaPhe: 3.423 ± 0.056
4.206AlaGly: 4.206 ± 0.081
1.076AlaHis: 1.076 ± 0.028
5.681AlaIle: 5.681 ± 0.07
5.037AlaLys: 5.037 ± 0.132
6.591AlaLeu: 6.591 ± 0.102
1.549AlaMet: 1.549 ± 0.046
3.728AlaAsn: 3.728 ± 0.074
1.976AlaPro: 1.976 ± 0.058
2.646AlaGln: 2.646 ± 0.055
1.889AlaArg: 1.889 ± 0.042
4.277AlaSer: 4.277 ± 0.068
4.362AlaThr: 4.362 ± 0.098
4.506AlaVal: 4.506 ± 0.071
0.575AlaTrp: 0.575 ± 0.023
2.277AlaTyr: 2.277 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.459CysAla: 0.459 ± 0.023
0.118CysCys: 0.118 ± 0.012
0.436CysAsp: 0.436 ± 0.019
0.436CysGlu: 0.436 ± 0.024
0.454CysPhe: 0.454 ± 0.022
0.583CysGly: 0.583 ± 0.026
0.188CysHis: 0.188 ± 0.014
0.669CysIle: 0.669 ± 0.027
0.506CysLys: 0.506 ± 0.022
0.685CysLeu: 0.685 ± 0.027
0.158CysMet: 0.158 ± 0.014
0.465CysAsn: 0.465 ± 0.023
0.347CysPro: 0.347 ± 0.027
0.257CysGln: 0.257 ± 0.014
0.179CysArg: 0.179 ± 0.014
0.609CysSer: 0.609 ± 0.028
0.485CysThr: 0.485 ± 0.026
0.451CysVal: 0.451 ± 0.024
0.063CysTrp: 0.063 ± 0.008
0.334CysTyr: 0.334 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.801AspAla: 3.801 ± 0.076
0.438AspCys: 0.438 ± 0.022
2.35AspAsp: 2.35 ± 0.053
3.351AspGlu: 3.351 ± 0.066
3.726AspPhe: 3.726 ± 0.069
3.249AspGly: 3.249 ± 0.063
0.787AspHis: 0.787 ± 0.026
3.981AspIle: 3.981 ± 0.06
4.239AspLys: 4.239 ± 0.063
5.185AspLeu: 5.185 ± 0.084
0.987AspMet: 0.987 ± 0.037
2.954AspAsn: 2.954 ± 0.06
1.537AspPro: 1.537 ± 0.047
1.608AspGln: 1.608 ± 0.034
1.748AspArg: 1.748 ± 0.038
2.892AspSer: 2.892 ± 0.053
2.692AspThr: 2.692 ± 0.047
3.456AspVal: 3.456 ± 0.06
0.64AspTrp: 0.64 ± 0.024
2.583AspTyr: 2.583 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
4.085GluAla: 4.085 ± 0.085
0.36GluCys: 0.36 ± 0.02
3.057GluAsp: 3.057 ± 0.061
4.39GluGlu: 4.39 ± 0.088
2.872GluPhe: 2.872 ± 0.056
3.286GluGly: 3.286 ± 0.061
1.011GluHis: 1.011 ± 0.035
5.763GluIle: 5.763 ± 0.085
6.068GluLys: 6.068 ± 0.087
5.899GluLeu: 5.899 ± 0.095
1.683GluMet: 1.683 ± 0.041
4.829GluAsn: 4.829 ± 0.068
1.463GluPro: 1.463 ± 0.042
2.328GluGln: 2.328 ± 0.051
2.291GluArg: 2.291 ± 0.05
3.313GluSer: 3.313 ± 0.055
3.638GluThr: 3.638 ± 0.066
4.02GluVal: 4.02 ± 0.069
0.579GluTrp: 0.579 ± 0.025
2.342GluTyr: 2.342 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
3.286PheAla: 3.286 ± 0.061
0.507PheCys: 0.507 ± 0.025
3.317PheAsp: 3.317 ± 0.062
3.731PheGlu: 3.731 ± 0.058
2.966PhePhe: 2.966 ± 0.058
3.634PheGly: 3.634 ± 0.064
0.882PheHis: 0.882 ± 0.031
4.075PheIle: 4.075 ± 0.074
3.879PheLys: 3.879 ± 0.065
4.835PheLeu: 4.835 ± 0.084
1.158PheMet: 1.158 ± 0.034
3.251PheAsn: 3.251 ± 0.069
1.777PhePro: 1.777 ± 0.044
1.756PheGln: 1.756 ± 0.043
1.579PheArg: 1.579 ± 0.043
4.057PheSer: 4.057 ± 0.073
3.34PheThr: 3.34 ± 0.072
3.323PheVal: 3.323 ± 0.06
0.584PheTrp: 0.584 ± 0.025
2.219PheTyr: 2.219 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
3.904GlyAla: 3.904 ± 0.068
0.593GlyCys: 0.593 ± 0.033
2.918GlyAsp: 2.918 ± 0.065
3.115GlyGlu: 3.115 ± 0.058
3.731GlyPhe: 3.731 ± 0.061
4.197GlyGly: 4.197 ± 0.082
1.041GlyHis: 1.041 ± 0.032
5.508GlyIle: 5.508 ± 0.079
4.988GlyLys: 4.988 ± 0.07
5.612GlyLeu: 5.612 ± 0.08
1.555GlyMet: 1.555 ± 0.042
3.607GlyAsn: 3.607 ± 0.066
1.247GlyPro: 1.247 ± 0.042
1.865GlyGln: 1.865 ± 0.044
1.873GlyArg: 1.873 ± 0.049
3.994GlySer: 3.994 ± 0.073
4.184GlyThr: 4.184 ± 0.084
4.113GlyVal: 4.113 ± 0.061
0.657GlyTrp: 0.657 ± 0.035
2.673GlyTyr: 2.673 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
0.955HisAla: 0.955 ± 0.033
0.195HisCys: 0.195 ± 0.015
0.841HisAsp: 0.841 ± 0.029
0.977HisGlu: 0.977 ± 0.033
1.213HisPhe: 1.213 ± 0.036
0.992HisGly: 0.992 ± 0.032
0.427HisHis: 0.427 ± 0.024
1.292HisIle: 1.292 ± 0.036
1.174HisLys: 1.174 ± 0.035
1.659HisLeu: 1.659 ± 0.047
0.276HisMet: 0.276 ± 0.019
0.945HisAsn: 0.945 ± 0.028
0.784HisPro: 0.784 ± 0.029
0.778HisGln: 0.778 ± 0.03
0.569HisArg: 0.569 ± 0.023
1.001HisSer: 1.001 ± 0.034
0.884HisThr: 0.884 ± 0.029
0.839HisVal: 0.839 ± 0.033
0.169HisTrp: 0.169 ± 0.012
0.771HisTyr: 0.771 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.123IleAla: 6.123 ± 0.09
0.641IleCys: 0.641 ± 0.027
4.818IleAsp: 4.818 ± 0.077
5.477IleGlu: 5.477 ± 0.092
3.76IlePhe: 3.76 ± 0.058
5.087IleGly: 5.087 ± 0.076
1.422IleHis: 1.422 ± 0.039
6.316IleIle: 6.316 ± 0.099
6.06IleLys: 6.06 ± 0.082
7.293IleLeu: 7.293 ± 0.109
1.518IleMet: 1.518 ± 0.039
4.827IleAsn: 4.827 ± 0.069
3.328IlePro: 3.328 ± 0.053
3.079IleGln: 3.079 ± 0.057
2.462IleArg: 2.462 ± 0.05
5.394IleSer: 5.394 ± 0.085
5.105IleThr: 5.105 ± 0.082
5.257IleVal: 5.257 ± 0.091
0.694IleTrp: 0.694 ± 0.027
2.602IleTyr: 2.602 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
5.177LysAla: 5.177 ± 0.123
0.428LysCys: 0.428 ± 0.021
4.164LysAsp: 4.164 ± 0.071
5.943LysGlu: 5.943 ± 0.092
3.124LysPhe: 3.124 ± 0.057
4.484LysGly: 4.484 ± 0.065
1.176LysHis: 1.176 ± 0.029
6.978LysIle: 6.978 ± 0.093
7.423LysLys: 7.423 ± 0.098
6.619LysLeu: 6.619 ± 0.076
2.241LysMet: 2.241 ± 0.052
5.931LysAsn: 5.931 ± 0.097
2.494LysPro: 2.494 ± 0.048
2.845LysGln: 2.845 ± 0.059
2.565LysArg: 2.565 ± 0.056
4.772LysSer: 4.772 ± 0.072
5.111LysThr: 5.111 ± 0.078
4.847LysVal: 4.847 ± 0.065
0.77LysTrp: 0.77 ± 0.025
3.04LysTyr: 3.04 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
6.0LeuAla: 6.0 ± 0.087
0.717LeuCys: 0.717 ± 0.026
4.974LeuAsp: 4.974 ± 0.078
6.113LeuGlu: 6.113 ± 0.085
5.163LeuPhe: 5.163 ± 0.097
5.717LeuGly: 5.717 ± 0.092
1.572LeuHis: 1.572 ± 0.043
6.979LeuIle: 6.979 ± 0.111
7.749LeuLys: 7.749 ± 0.092
8.883LeuLeu: 8.883 ± 0.131
2.057LeuMet: 2.057 ± 0.049
5.745LeuAsn: 5.745 ± 0.082
3.433LeuPro: 3.433 ± 0.062
3.663LeuGln: 3.663 ± 0.068
2.974LeuArg: 2.974 ± 0.059
6.653LeuSer: 6.653 ± 0.09
5.105LeuThr: 5.105 ± 0.091
5.434LeuVal: 5.434 ± 0.073
0.704LeuTrp: 0.704 ± 0.027
3.299LeuTyr: 3.299 ± 0.061
0.0LeuXaa: 0.0 ± 0.0
Met
1.513MetAla: 1.513 ± 0.045
0.144MetCys: 0.144 ± 0.011
1.108MetAsp: 1.108 ± 0.035
1.354MetGlu: 1.354 ± 0.039
0.867MetPhe: 0.867 ± 0.033
1.364MetGly: 1.364 ± 0.036
0.39MetHis: 0.39 ± 0.02
1.659MetIle: 1.659 ± 0.043
2.295MetLys: 2.295 ± 0.04
1.855MetLeu: 1.855 ± 0.043
0.608MetMet: 0.608 ± 0.029
1.42MetAsn: 1.42 ± 0.032
0.851MetPro: 0.851 ± 0.031
0.937MetGln: 0.937 ± 0.033
0.794MetArg: 0.794 ± 0.031
1.407MetSer: 1.407 ± 0.036
1.221MetThr: 1.221 ± 0.035
1.346MetVal: 1.346 ± 0.037
0.158MetTrp: 0.158 ± 0.013
0.728MetTyr: 0.728 ± 0.029
0.001MetXaa: 0.001 ± 0.001
Asn
4.13AsnAla: 4.13 ± 0.067
0.534AsnCys: 0.534 ± 0.026
3.167AsnAsp: 3.167 ± 0.062
3.942AsnGlu: 3.942 ± 0.069
3.337AsnPhe: 3.337 ± 0.068
4.109AsnGly: 4.109 ± 0.083
1.068AsnHis: 1.068 ± 0.031
4.761AsnIle: 4.761 ± 0.078
4.706AsnLys: 4.706 ± 0.086
5.629AsnLeu: 5.629 ± 0.08
1.197AsnMet: 1.197 ± 0.034
4.104AsnAsn: 4.104 ± 0.095
2.899AsnPro: 2.899 ± 0.058
2.51AsnGln: 2.51 ± 0.054
2.021AsnArg: 2.021 ± 0.052
4.211AsnSer: 4.211 ± 0.082
3.935AsnThr: 3.935 ± 0.078
3.639AsnVal: 3.639 ± 0.073
0.825AsnTrp: 0.825 ± 0.027
2.84AsnTyr: 2.84 ± 0.077
0.0AsnXaa: 0.0 ± 0.0
Pro
2.143ProAla: 2.143 ± 0.049
0.209ProCys: 0.209 ± 0.014
1.802ProAsp: 1.802 ± 0.043
2.634ProGlu: 2.634 ± 0.051
1.993ProPhe: 1.993 ± 0.045
1.691ProGly: 1.691 ± 0.038
0.538ProHis: 0.538 ± 0.024
2.656ProIle: 2.656 ± 0.051
2.551ProLys: 2.551 ± 0.051
2.978ProLeu: 2.978 ± 0.058
0.702ProMet: 0.702 ± 0.026
2.339ProAsn: 2.339 ± 0.054
0.738ProPro: 0.738 ± 0.032
1.132ProGln: 1.132 ± 0.033
0.866ProArg: 0.866 ± 0.032
2.136ProSer: 2.136 ± 0.051
2.143ProThr: 2.143 ± 0.055
2.355ProVal: 2.355 ± 0.064
0.306ProTrp: 0.306 ± 0.017
1.285ProTyr: 1.285 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.153GlnAla: 2.153 ± 0.051
0.211GlnCys: 0.211 ± 0.014
1.792GlnAsp: 1.792 ± 0.036
2.545GlnGlu: 2.545 ± 0.056
1.947GlnPhe: 1.947 ± 0.046
1.79GlnGly: 1.79 ± 0.045
0.601GlnHis: 0.601 ± 0.023
2.955GlnIle: 2.955 ± 0.049
3.412GlnLys: 3.412 ± 0.067
3.694GlnLeu: 3.694 ± 0.057
0.826GlnMet: 0.826 ± 0.031
2.671GlnAsn: 2.671 ± 0.061
1.067GlnPro: 1.067 ± 0.029
1.475GlnGln: 1.475 ± 0.037
1.158GlnArg: 1.158 ± 0.034
2.131GlnSer: 2.131 ± 0.051
2.032GlnThr: 2.032 ± 0.046
2.11GlnVal: 2.11 ± 0.048
0.35GlnTrp: 0.35 ± 0.018
1.429GlnTyr: 1.429 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
1.859ArgAla: 1.859 ± 0.052
0.2ArgCys: 0.2 ± 0.013
1.66ArgAsp: 1.66 ± 0.039
2.041ArgGlu: 2.041 ± 0.049
1.873ArgPhe: 1.873 ± 0.043
1.752ArgGly: 1.752 ± 0.038
0.516ArgHis: 0.516 ± 0.023
2.728ArgIle: 2.728 ± 0.058
2.589ArgLys: 2.589 ± 0.055
2.979ArgLeu: 2.979 ± 0.058
0.849ArgMet: 0.849 ± 0.032
2.087ArgAsn: 2.087 ± 0.044
0.934ArgPro: 0.934 ± 0.031
0.955ArgGln: 0.955 ± 0.031
1.104ArgArg: 1.104 ± 0.039
1.778ArgSer: 1.778 ± 0.048
1.74ArgThr: 1.74 ± 0.038
2.06ArgVal: 2.06 ± 0.05
0.358ArgTrp: 0.358 ± 0.019
1.376ArgTyr: 1.376 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
3.84SerAla: 3.84 ± 0.071
0.657SerCys: 0.657 ± 0.028
3.209SerAsp: 3.209 ± 0.056
3.613SerGlu: 3.613 ± 0.065
3.968SerPhe: 3.968 ± 0.074
4.487SerGly: 4.487 ± 0.09
1.147SerHis: 1.147 ± 0.032
5.35SerIle: 5.35 ± 0.082
5.165SerLys: 5.165 ± 0.087
5.978SerLeu: 5.978 ± 0.093
1.324SerMet: 1.324 ± 0.036
4.055SerAsn: 4.055 ± 0.082
2.013SerPro: 2.013 ± 0.045
2.423SerGln: 2.423 ± 0.048
2.062SerArg: 2.062 ± 0.055
4.367SerSer: 4.367 ± 0.076
3.604SerThr: 3.604 ± 0.073
4.086SerVal: 4.086 ± 0.066
0.677SerTrp: 0.677 ± 0.026
2.653SerTyr: 2.653 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
4.371ThrAla: 4.371 ± 0.084
0.386ThrCys: 0.386 ± 0.025
3.169ThrAsp: 3.169 ± 0.055
3.321ThrGlu: 3.321 ± 0.062
3.18ThrPhe: 3.18 ± 0.054
4.011ThrGly: 4.011 ± 0.085
0.98ThrHis: 0.98 ± 0.031
5.451ThrIle: 5.451 ± 0.08
4.185ThrLys: 4.185 ± 0.076
5.557ThrLeu: 5.557 ± 0.077
1.011ThrMet: 1.011 ± 0.034
3.645ThrAsn: 3.645 ± 0.083
2.532ThrPro: 2.532 ± 0.057
2.112ThrGln: 2.112 ± 0.045
1.641ThrArg: 1.641 ± 0.044
3.924ThrSer: 3.924 ± 0.08
4.09ThrThr: 4.09 ± 0.094
4.256ThrVal: 4.256 ± 0.093
0.49ThrTrp: 0.49 ± 0.021
2.243ThrTyr: 2.243 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
4.63ValAla: 4.63 ± 0.08
0.556ValCys: 0.556 ± 0.027
3.39ValAsp: 3.39 ± 0.056
3.623ValGlu: 3.623 ± 0.064
3.506ValPhe: 3.506 ± 0.069
3.708ValGly: 3.708 ± 0.076
0.982ValHis: 0.982 ± 0.029
5.003ValIle: 5.003 ± 0.08
4.485ValLys: 4.485 ± 0.069
6.412ValLeu: 6.412 ± 0.084
1.364ValMet: 1.364 ± 0.04
3.678ValAsn: 3.678 ± 0.062
2.194ValPro: 2.194 ± 0.053
2.039ValGln: 2.039 ± 0.041
1.906ValArg: 1.906 ± 0.049
4.447ValSer: 4.447 ± 0.077
3.942ValThr: 3.942 ± 0.085
4.434ValVal: 4.434 ± 0.081
0.572ValTrp: 0.572 ± 0.022
2.358ValTyr: 2.358 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.604TrpAla: 0.604 ± 0.026
0.091TrpCys: 0.091 ± 0.009
0.53TrpAsp: 0.53 ± 0.022
0.596TrpGlu: 0.596 ± 0.025
0.556TrpPhe: 0.556 ± 0.022
0.571TrpGly: 0.571 ± 0.027
0.194TrpHis: 0.194 ± 0.013
0.767TrpIle: 0.767 ± 0.029
0.771TrpLys: 0.771 ± 0.03
0.881TrpLeu: 0.881 ± 0.034
0.249TrpMet: 0.249 ± 0.019
0.707TrpAsn: 0.707 ± 0.032
0.202TrpPro: 0.202 ± 0.016
0.373TrpGln: 0.373 ± 0.02
0.355TrpArg: 0.355 ± 0.017
0.643TrpSer: 0.643 ± 0.027
0.547TrpThr: 0.547 ± 0.025
0.535TrpVal: 0.535 ± 0.019
0.136TrpTrp: 0.136 ± 0.012
0.417TrpTyr: 0.417 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.407TyrAla: 2.407 ± 0.049
0.33TyrCys: 0.33 ± 0.017
2.212TyrAsp: 2.212 ± 0.053
2.288TyrGlu: 2.288 ± 0.046
2.499TyrPhe: 2.499 ± 0.055
2.458TyrGly: 2.458 ± 0.051
0.744TyrHis: 0.744 ± 0.028
2.691TyrIle: 2.691 ± 0.052
2.847TyrLys: 2.847 ± 0.05
3.709TyrLeu: 3.709 ± 0.062
0.71TyrMet: 0.71 ± 0.026
2.577TyrAsn: 2.577 ± 0.06
1.383TyrPro: 1.383 ± 0.037
1.568TyrGln: 1.568 ± 0.044
1.402TyrArg: 1.402 ± 0.041
2.667TyrSer: 2.667 ± 0.052
2.393TyrThr: 2.393 ± 0.059
2.164TyrVal: 2.164 ± 0.041
0.42TyrTrp: 0.42 ± 0.019
1.708TyrTyr: 1.708 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.001XaaSer: 0.001 ± 0.001
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.001XaaXaa: 0.001 ± 0.001
Statistics based on 3173 proteins (1053902 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski