Amino acid dipepetide frequency for Brevibacterium phage AGM3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.266AlaAla: 12.266 ± 2.24
0.777AlaCys: 0.777 ± 0.262
5.787AlaAsp: 5.787 ± 0.919
6.91AlaGlu: 6.91 ± 0.792
2.764AlaPhe: 2.764 ± 0.447
9.502AlaGly: 9.502 ± 1.323
1.123AlaHis: 1.123 ± 0.374
4.319AlaIle: 4.319 ± 0.553
4.751AlaLys: 4.751 ± 0.97
9.415AlaLeu: 9.415 ± 1.021
2.764AlaMet: 2.764 ± 0.57
3.542AlaAsn: 3.542 ± 0.595
3.801AlaPro: 3.801 ± 0.637
4.319AlaGln: 4.319 ± 0.51
7.256AlaArg: 7.256 ± 0.788
5.96AlaSer: 5.96 ± 0.71
6.478AlaThr: 6.478 ± 0.615
6.306AlaVal: 6.306 ± 0.883
1.555AlaTrp: 1.555 ± 0.318
2.505AlaTyr: 2.505 ± 0.505
0.0AlaXaa: 0.0 ± 0.0
Cys
1.037CysAla: 1.037 ± 0.298
0.259CysCys: 0.259 ± 0.163
1.123CysAsp: 1.123 ± 0.439
0.346CysGlu: 0.346 ± 0.163
0.173CysPhe: 0.173 ± 0.133
0.777CysGly: 0.777 ± 0.269
0.432CysHis: 0.432 ± 0.248
0.0CysIle: 0.0 ± 0.0
0.864CysLys: 0.864 ± 0.294
0.605CysLeu: 0.605 ± 0.26
0.346CysMet: 0.346 ± 0.227
0.173CysAsn: 0.173 ± 0.135
0.691CysPro: 0.691 ± 0.286
0.346CysGln: 0.346 ± 0.209
0.777CysArg: 0.777 ± 0.386
0.259CysSer: 0.259 ± 0.16
0.259CysThr: 0.259 ± 0.14
0.691CysVal: 0.691 ± 0.342
0.173CysTrp: 0.173 ± 0.112
0.346CysTyr: 0.346 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
6.133AspAla: 6.133 ± 0.764
1.123AspCys: 1.123 ± 0.512
3.887AspAsp: 3.887 ± 0.757
3.196AspGlu: 3.196 ± 0.562
1.641AspPhe: 1.641 ± 0.531
6.997AspGly: 6.997 ± 1.129
1.123AspHis: 1.123 ± 0.352
2.073AspIle: 2.073 ± 0.413
2.246AspLys: 2.246 ± 0.534
5.269AspLeu: 5.269 ± 0.636
1.037AspMet: 1.037 ± 0.322
2.073AspAsn: 2.073 ± 0.557
3.801AspPro: 3.801 ± 0.663
2.591AspGln: 2.591 ± 0.43
5.096AspArg: 5.096 ± 0.782
3.973AspSer: 3.973 ± 0.527
5.528AspThr: 5.528 ± 1.045
4.492AspVal: 4.492 ± 0.66
0.95AspTrp: 0.95 ± 0.293
1.814AspTyr: 1.814 ± 0.309
0.0AspXaa: 0.0 ± 0.0
Glu
8.379GluAla: 8.379 ± 0.897
0.259GluCys: 0.259 ± 0.122
3.11GluAsp: 3.11 ± 0.62
4.664GluGlu: 4.664 ± 0.788
1.209GluPhe: 1.209 ± 0.424
4.146GluGly: 4.146 ± 0.735
1.468GluHis: 1.468 ± 0.412
3.11GluIle: 3.11 ± 0.381
2.419GluLys: 2.419 ± 0.523
4.06GluLeu: 4.06 ± 0.609
1.037GluMet: 1.037 ± 0.344
2.332GluAsn: 2.332 ± 0.593
2.678GluPro: 2.678 ± 0.715
3.196GluGln: 3.196 ± 0.582
4.924GluArg: 4.924 ± 0.581
3.542GluSer: 3.542 ± 0.736
3.973GluThr: 3.973 ± 0.575
3.542GluVal: 3.542 ± 0.635
0.777GluTrp: 0.777 ± 0.296
1.987GluTyr: 1.987 ± 0.48
0.0GluXaa: 0.0 ± 0.0
Phe
2.505PheAla: 2.505 ± 0.422
0.173PheCys: 0.173 ± 0.118
2.332PheAsp: 2.332 ± 0.472
1.728PheGlu: 1.728 ± 0.487
0.432PhePhe: 0.432 ± 0.239
2.937PheGly: 2.937 ± 0.485
0.777PheHis: 0.777 ± 0.297
1.468PheIle: 1.468 ± 0.535
1.382PheLys: 1.382 ± 0.303
1.382PheLeu: 1.382 ± 0.366
0.518PheMet: 0.518 ± 0.18
0.777PheAsn: 0.777 ± 0.228
1.9PhePro: 1.9 ± 0.319
0.605PheGln: 0.605 ± 0.265
1.209PheArg: 1.209 ± 0.37
1.641PheSer: 1.641 ± 0.451
2.159PheThr: 2.159 ± 0.432
1.728PheVal: 1.728 ± 0.484
0.173PheTrp: 0.173 ± 0.144
0.777PheTyr: 0.777 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
7.688GlyAla: 7.688 ± 0.856
0.691GlyCys: 0.691 ± 0.257
5.355GlyAsp: 5.355 ± 0.639
4.06GlyGlu: 4.06 ± 0.691
3.369GlyPhe: 3.369 ± 0.714
7.429GlyGly: 7.429 ± 1.708
1.123GlyHis: 1.123 ± 0.298
4.06GlyIle: 4.06 ± 0.958
3.714GlyLys: 3.714 ± 0.707
7.774GlyLeu: 7.774 ± 0.791
2.85GlyMet: 2.85 ± 0.626
3.801GlyAsn: 3.801 ± 0.9
4.233GlyPro: 4.233 ± 1.315
2.764GlyGln: 2.764 ± 0.499
5.442GlyArg: 5.442 ± 0.718
6.392GlySer: 6.392 ± 1.094
5.269GlyThr: 5.269 ± 0.89
5.269GlyVal: 5.269 ± 0.913
2.419GlyTrp: 2.419 ± 0.537
3.282GlyTyr: 3.282 ± 0.541
0.0GlyXaa: 0.0 ± 0.0
His
1.728HisAla: 1.728 ± 0.414
0.086HisCys: 0.086 ± 0.085
1.123HisAsp: 1.123 ± 0.323
0.605HisGlu: 0.605 ± 0.296
0.864HisPhe: 0.864 ± 0.32
1.296HisGly: 1.296 ± 0.405
0.432HisHis: 0.432 ± 0.142
0.864HisIle: 0.864 ± 0.238
1.037HisLys: 1.037 ± 0.242
1.468HisLeu: 1.468 ± 0.342
0.346HisMet: 0.346 ± 0.169
0.346HisAsn: 0.346 ± 0.177
2.505HisPro: 2.505 ± 0.978
0.95HisGln: 0.95 ± 0.302
0.95HisArg: 0.95 ± 0.371
0.777HisSer: 0.777 ± 0.232
1.468HisThr: 1.468 ± 0.457
1.037HisVal: 1.037 ± 0.445
0.432HisTrp: 0.432 ± 0.189
0.777HisTyr: 0.777 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
4.751IleAla: 4.751 ± 0.713
0.605IleCys: 0.605 ± 0.378
3.196IleAsp: 3.196 ± 0.372
3.196IleGlu: 3.196 ± 0.475
1.037IlePhe: 1.037 ± 0.392
3.801IleGly: 3.801 ± 0.594
0.864IleHis: 0.864 ± 0.208
1.987IleIle: 1.987 ± 0.443
1.555IleLys: 1.555 ± 0.309
3.11IleLeu: 3.11 ± 0.486
0.777IleMet: 0.777 ± 0.194
1.555IleAsn: 1.555 ± 0.404
2.764IlePro: 2.764 ± 0.498
1.641IleGln: 1.641 ± 0.272
3.369IleArg: 3.369 ± 0.544
2.678IleSer: 2.678 ± 0.47
3.801IleThr: 3.801 ± 0.556
3.196IleVal: 3.196 ± 0.556
0.605IleTrp: 0.605 ± 0.222
0.777IleTyr: 0.777 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
4.233LysAla: 4.233 ± 1.077
0.605LysCys: 0.605 ± 0.292
3.11LysAsp: 3.11 ± 0.5
2.332LysGlu: 2.332 ± 0.44
1.555LysPhe: 1.555 ± 0.431
3.628LysGly: 3.628 ± 0.791
1.209LysHis: 1.209 ± 0.356
2.419LysIle: 2.419 ± 0.395
2.764LysLys: 2.764 ± 0.638
3.196LysLeu: 3.196 ± 0.671
1.641LysMet: 1.641 ± 0.353
1.468LysAsn: 1.468 ± 0.412
2.505LysPro: 2.505 ± 0.576
1.987LysGln: 1.987 ± 0.455
3.023LysArg: 3.023 ± 0.547
2.419LysSer: 2.419 ± 0.425
2.937LysThr: 2.937 ± 0.945
3.11LysVal: 3.11 ± 0.643
0.691LysTrp: 0.691 ± 0.228
1.123LysTyr: 1.123 ± 0.367
0.0LysXaa: 0.0 ± 0.0
Leu
7.774LeuAla: 7.774 ± 0.915
0.95LeuCys: 0.95 ± 0.399
4.492LeuAsp: 4.492 ± 0.533
4.146LeuGlu: 4.146 ± 0.6
1.209LeuPhe: 1.209 ± 0.403
6.997LeuGly: 6.997 ± 1.027
1.641LeuHis: 1.641 ± 0.326
2.764LeuIle: 2.764 ± 0.683
3.628LeuLys: 3.628 ± 0.646
4.837LeuLeu: 4.837 ± 0.561
1.209LeuMet: 1.209 ± 0.266
2.678LeuAsn: 2.678 ± 0.441
3.196LeuPro: 3.196 ± 0.555
2.505LeuGln: 2.505 ± 0.426
6.478LeuArg: 6.478 ± 0.993
5.874LeuSer: 5.874 ± 0.752
5.787LeuThr: 5.787 ± 0.656
5.01LeuVal: 5.01 ± 0.673
0.95LeuTrp: 0.95 ± 0.264
1.641LeuTyr: 1.641 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
2.591MetAla: 2.591 ± 0.502
0.086MetCys: 0.086 ± 0.095
1.296MetAsp: 1.296 ± 0.338
1.209MetGlu: 1.209 ± 0.367
0.346MetPhe: 0.346 ± 0.209
1.987MetGly: 1.987 ± 0.558
0.173MetHis: 0.173 ± 0.125
1.037MetIle: 1.037 ± 0.282
1.468MetLys: 1.468 ± 0.351
1.382MetLeu: 1.382 ± 0.452
0.346MetMet: 0.346 ± 0.153
0.95MetAsn: 0.95 ± 0.256
0.864MetPro: 0.864 ± 0.301
0.864MetGln: 0.864 ± 0.365
2.073MetArg: 2.073 ± 0.407
2.85MetSer: 2.85 ± 0.727
2.073MetThr: 2.073 ± 0.416
1.555MetVal: 1.555 ± 0.365
0.864MetTrp: 0.864 ± 0.323
0.346MetTyr: 0.346 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.023AsnAla: 3.023 ± 0.637
0.259AsnCys: 0.259 ± 0.196
1.728AsnAsp: 1.728 ± 0.554
2.937AsnGlu: 2.937 ± 0.689
1.037AsnPhe: 1.037 ± 0.33
3.282AsnGly: 3.282 ± 0.551
0.777AsnHis: 0.777 ± 0.259
1.555AsnIle: 1.555 ± 0.387
1.555AsnLys: 1.555 ± 0.324
2.764AsnLeu: 2.764 ± 0.617
0.518AsnMet: 0.518 ± 0.224
1.037AsnAsn: 1.037 ± 0.285
1.987AsnPro: 1.987 ± 0.541
1.468AsnGln: 1.468 ± 0.396
2.246AsnArg: 2.246 ± 0.445
2.073AsnSer: 2.073 ± 0.418
3.282AsnThr: 3.282 ± 0.587
1.555AsnVal: 1.555 ± 0.346
0.518AsnTrp: 0.518 ± 0.246
0.95AsnTyr: 0.95 ± 0.345
0.0AsnXaa: 0.0 ± 0.0
Pro
6.133ProAla: 6.133 ± 1.744
0.518ProCys: 0.518 ± 0.24
3.714ProAsp: 3.714 ± 1.032
3.196ProGlu: 3.196 ± 0.557
0.777ProPhe: 0.777 ± 0.284
4.06ProGly: 4.06 ± 0.601
0.95ProHis: 0.95 ± 0.277
1.9ProIle: 1.9 ± 0.434
2.764ProLys: 2.764 ± 1.018
2.85ProLeu: 2.85 ± 0.599
1.9ProMet: 1.9 ± 0.411
2.246ProAsn: 2.246 ± 0.522
3.196ProPro: 3.196 ± 0.725
1.641ProGln: 1.641 ± 0.329
2.764ProArg: 2.764 ± 0.707
3.369ProSer: 3.369 ± 0.75
3.282ProThr: 3.282 ± 0.685
4.924ProVal: 4.924 ± 1.587
0.605ProTrp: 0.605 ± 0.22
1.123ProTyr: 1.123 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
4.319GlnAla: 4.319 ± 0.825
0.346GlnCys: 0.346 ± 0.185
2.332GlnAsp: 2.332 ± 0.46
1.728GlnGlu: 1.728 ± 0.406
1.296GlnPhe: 1.296 ± 0.348
2.419GlnGly: 2.419 ± 0.467
0.95GlnHis: 0.95 ± 0.295
2.159GlnIle: 2.159 ± 0.428
1.9GlnLys: 1.9 ± 0.358
2.937GlnLeu: 2.937 ± 0.478
0.864GlnMet: 0.864 ± 0.221
1.209GlnAsn: 1.209 ± 0.373
1.037GlnPro: 1.037 ± 0.248
1.382GlnGln: 1.382 ± 0.424
2.764GlnArg: 2.764 ± 0.527
3.369GlnSer: 3.369 ± 0.98
1.9GlnThr: 1.9 ± 0.431
2.246GlnVal: 2.246 ± 0.423
0.864GlnTrp: 0.864 ± 0.247
1.037GlnTyr: 1.037 ± 0.351
0.0GlnXaa: 0.0 ± 0.0
Arg
6.133ArgAla: 6.133 ± 0.71
0.95ArgCys: 0.95 ± 0.342
4.751ArgAsp: 4.751 ± 1.011
4.319ArgGlu: 4.319 ± 0.617
1.9ArgPhe: 1.9 ± 0.434
5.01ArgGly: 5.01 ± 0.628
1.814ArgHis: 1.814 ± 0.441
4.146ArgIle: 4.146 ± 0.638
3.542ArgLys: 3.542 ± 0.614
5.269ArgLeu: 5.269 ± 0.675
1.814ArgMet: 1.814 ± 0.395
2.332ArgAsn: 2.332 ± 0.408
2.678ArgPro: 2.678 ± 0.645
2.419ArgGln: 2.419 ± 0.438
6.478ArgArg: 6.478 ± 1.081
5.355ArgSer: 5.355 ± 0.648
4.146ArgThr: 4.146 ± 0.631
4.924ArgVal: 4.924 ± 0.78
0.777ArgTrp: 0.777 ± 0.325
1.728ArgTyr: 1.728 ± 0.355
0.0ArgXaa: 0.0 ± 0.0
Ser
6.91SerAla: 6.91 ± 0.733
0.518SerCys: 0.518 ± 0.199
3.542SerAsp: 3.542 ± 0.587
3.887SerGlu: 3.887 ± 0.549
1.728SerPhe: 1.728 ± 0.358
7.774SerGly: 7.774 ± 1.221
0.432SerHis: 0.432 ± 0.216
3.023SerIle: 3.023 ± 0.466
3.369SerLys: 3.369 ± 0.544
3.801SerLeu: 3.801 ± 0.774
2.419SerMet: 2.419 ± 0.424
2.246SerAsn: 2.246 ± 0.41
3.714SerPro: 3.714 ± 1.103
2.332SerGln: 2.332 ± 0.699
3.973SerArg: 3.973 ± 0.677
5.096SerSer: 5.096 ± 0.846
5.96SerThr: 5.96 ± 0.724
4.578SerVal: 4.578 ± 0.538
0.777SerTrp: 0.777 ± 0.263
1.382SerTyr: 1.382 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
6.91ThrAla: 6.91 ± 0.971
0.518ThrCys: 0.518 ± 0.207
5.01ThrAsp: 5.01 ± 0.825
3.887ThrGlu: 3.887 ± 0.647
2.159ThrPhe: 2.159 ± 0.52
6.737ThrGly: 6.737 ± 1.187
1.123ThrHis: 1.123 ± 0.341
3.455ThrIle: 3.455 ± 0.444
2.505ThrLys: 2.505 ± 0.365
5.874ThrLeu: 5.874 ± 0.693
1.728ThrMet: 1.728 ± 0.56
2.159ThrAsn: 2.159 ± 0.458
4.492ThrPro: 4.492 ± 0.728
2.591ThrGln: 2.591 ± 0.719
2.937ThrArg: 2.937 ± 0.791
4.924ThrSer: 4.924 ± 0.584
5.355ThrThr: 5.355 ± 0.81
5.01ThrVal: 5.01 ± 0.618
1.037ThrTrp: 1.037 ± 0.267
1.814ThrTyr: 1.814 ± 0.476
0.0ThrXaa: 0.0 ± 0.0
Val
5.787ValAla: 5.787 ± 0.677
0.605ValCys: 0.605 ± 0.25
5.874ValAsp: 5.874 ± 0.695
5.01ValGlu: 5.01 ± 0.759
1.728ValPhe: 1.728 ± 0.479
4.664ValGly: 4.664 ± 0.726
1.987ValHis: 1.987 ± 0.806
3.369ValIle: 3.369 ± 0.513
2.332ValLys: 2.332 ± 0.574
5.096ValLeu: 5.096 ± 0.763
1.641ValMet: 1.641 ± 0.382
2.159ValAsn: 2.159 ± 0.391
3.973ValPro: 3.973 ± 0.712
2.073ValGln: 2.073 ± 0.434
5.355ValArg: 5.355 ± 0.914
4.233ValSer: 4.233 ± 0.675
3.628ValThr: 3.628 ± 0.767
4.578ValVal: 4.578 ± 0.85
1.728ValTrp: 1.728 ± 0.464
1.296ValTyr: 1.296 ± 0.306
0.0ValXaa: 0.0 ± 0.0
Trp
1.728TrpAla: 1.728 ± 0.469
0.173TrpCys: 0.173 ± 0.129
1.382TrpAsp: 1.382 ± 0.4
1.555TrpGlu: 1.555 ± 0.413
0.346TrpPhe: 0.346 ± 0.193
0.777TrpGly: 0.777 ± 0.262
0.086TrpHis: 0.086 ± 0.093
0.864TrpIle: 0.864 ± 0.209
1.209TrpLys: 1.209 ± 0.297
1.209TrpLeu: 1.209 ± 0.389
0.173TrpMet: 0.173 ± 0.094
0.605TrpAsn: 0.605 ± 0.291
0.432TrpPro: 0.432 ± 0.218
0.605TrpGln: 0.605 ± 0.201
1.468TrpArg: 1.468 ± 0.392
1.209TrpSer: 1.209 ± 0.433
1.209TrpThr: 1.209 ± 0.335
1.123TrpVal: 1.123 ± 0.268
0.173TrpTrp: 0.173 ± 0.118
0.086TrpTyr: 0.086 ± 0.093
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.073TyrAla: 2.073 ± 0.374
0.173TyrCys: 0.173 ± 0.136
1.9TyrAsp: 1.9 ± 0.568
1.9TyrGlu: 1.9 ± 0.526
1.037TyrPhe: 1.037 ± 0.325
2.764TyrGly: 2.764 ± 0.49
0.605TyrHis: 0.605 ± 0.226
0.691TyrIle: 0.691 ± 0.269
0.95TyrLys: 0.95 ± 0.316
1.468TyrLeu: 1.468 ± 0.461
0.346TyrMet: 0.346 ± 0.142
0.864TyrAsn: 0.864 ± 0.31
1.468TyrPro: 1.468 ± 0.413
0.864TyrGln: 0.864 ± 0.289
1.9TyrArg: 1.9 ± 0.526
1.468TyrSer: 1.468 ± 0.624
1.728TyrThr: 1.728 ± 0.514
2.246TyrVal: 2.246 ± 0.457
0.346TyrTrp: 0.346 ± 0.154
0.346TyrTyr: 0.346 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (11578 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski