Lettuce chlorosis virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Crinivirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6FJ61|C6FJ61_9CLOS ORF5 p60 OS=Lettuce chlorosis virus OX=642478 PE=4 SV=1
MM1 pKa = 7.35GLKK4 pKa = 9.82IVLYY8 pKa = 9.58YY9 pKa = 8.68FNCGFSLYY17 pKa = 9.95YY18 pKa = 10.8CSDD21 pKa = 2.87NRR23 pKa = 11.84LFEE26 pKa = 4.34GQVDD30 pKa = 4.44TIYY33 pKa = 10.98TEE35 pKa = 5.62DD36 pKa = 3.46IQEE39 pKa = 4.64LIEE42 pKa = 4.83IINNFPFVKK51 pKa = 9.44TQWW54 pKa = 3.11

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6FJ56|C6FJ56_9CLOS p23 OS=Lettuce chlorosis virus OX=642478 PE=4 SV=1
MM1 pKa = 7.49IYY3 pKa = 10.29VYY5 pKa = 9.9PAYY8 pKa = 9.92CDD10 pKa = 4.88KK11 pKa = 10.34EE12 pKa = 4.33TQTYY16 pKa = 10.22NKK18 pKa = 9.1SLCGFYY24 pKa = 8.12TTFEE28 pKa = 4.42WIGFILILLILLFFICYY45 pKa = 9.19VVSSYY50 pKa = 7.57TLRR53 pKa = 11.84VRR55 pKa = 11.84NSRR58 pKa = 11.84WRR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 3.78IPIVV66 pKa = 3.38

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

7056

42

2495

504.0

57.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.294 ± 0.354

2.282 ± 0.255

6.236 ± 0.299

5.371 ± 0.259

5.102 ± 0.297

5.003 ± 0.185

2.225 ± 0.203

6.463 ± 0.327

7.937 ± 0.214

8.844 ± 0.503

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.948 ± 0.173

6.222 ± 0.293

3.373 ± 0.349

2.891 ± 0.143

4.776 ± 0.208

7.526 ± 0.225

5.442 ± 0.241

7.299 ± 0.403

0.865 ± 0.099

4.904 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski