Amino acid dipepetide frequency for Mycobacterium phage Malec

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.826AlaAla: 11.826 ± 1.059
0.425AlaCys: 0.425 ± 0.147
5.519AlaAsp: 5.519 ± 0.555
8.491AlaGlu: 8.491 ± 0.767
3.76AlaPhe: 3.76 ± 0.542
8.187AlaGly: 8.187 ± 0.919
1.577AlaHis: 1.577 ± 0.31
3.639AlaIle: 3.639 ± 0.411
5.276AlaLys: 5.276 ± 0.59
9.158AlaLeu: 9.158 ± 1.13
2.79AlaMet: 2.79 ± 0.416
2.972AlaAsn: 2.972 ± 0.541
4.67AlaPro: 4.67 ± 0.656
4.791AlaGln: 4.791 ± 0.743
6.61AlaArg: 6.61 ± 0.656
4.609AlaSer: 4.609 ± 0.494
5.398AlaThr: 5.398 ± 0.606
8.248AlaVal: 8.248 ± 0.684
1.88AlaTrp: 1.88 ± 0.36
2.668AlaTyr: 2.668 ± 0.459
0.0AlaXaa: 0.0 ± 0.0
Cys
0.728CysAla: 0.728 ± 0.207
0.0CysCys: 0.0 ± 0.0
0.849CysAsp: 0.849 ± 0.273
0.243CysGlu: 0.243 ± 0.119
0.425CysPhe: 0.425 ± 0.154
0.788CysGly: 0.788 ± 0.226
0.243CysHis: 0.243 ± 0.144
0.303CysIle: 0.303 ± 0.133
0.91CysLys: 0.91 ± 0.228
0.728CysLeu: 0.728 ± 0.201
0.182CysMet: 0.182 ± 0.11
0.425CysAsn: 0.425 ± 0.138
0.485CysPro: 0.485 ± 0.189
0.182CysGln: 0.182 ± 0.107
0.546CysArg: 0.546 ± 0.188
0.121CysSer: 0.121 ± 0.082
0.606CysThr: 0.606 ± 0.212
0.485CysVal: 0.485 ± 0.15
0.303CysTrp: 0.303 ± 0.155
0.182CysTyr: 0.182 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
6.55AspAla: 6.55 ± 0.607
0.728AspCys: 0.728 ± 0.217
3.699AspAsp: 3.699 ± 0.476
5.216AspGlu: 5.216 ± 0.57
2.85AspPhe: 2.85 ± 0.467
5.034AspGly: 5.034 ± 0.747
1.152AspHis: 1.152 ± 0.3
3.639AspIle: 3.639 ± 0.496
2.608AspLys: 2.608 ± 0.353
5.337AspLeu: 5.337 ± 0.734
1.577AspMet: 1.577 ± 0.283
1.213AspAsn: 1.213 ± 0.265
5.155AspPro: 5.155 ± 0.601
2.001AspGln: 2.001 ± 0.275
2.547AspArg: 2.547 ± 0.416
3.275AspSer: 3.275 ± 0.448
2.972AspThr: 2.972 ± 0.395
4.427AspVal: 4.427 ± 0.529
1.334AspTrp: 1.334 ± 0.261
1.941AspTyr: 1.941 ± 0.293
0.0AspXaa: 0.0 ± 0.0
Glu
6.732GluAla: 6.732 ± 0.685
0.606GluCys: 0.606 ± 0.217
5.216GluAsp: 5.216 ± 0.841
4.852GluGlu: 4.852 ± 0.756
2.668GluPhe: 2.668 ± 0.447
4.548GluGly: 4.548 ± 0.551
2.001GluHis: 2.001 ± 0.396
4.003GluIle: 4.003 ± 0.448
2.608GluLys: 2.608 ± 0.34
7.581GluLeu: 7.581 ± 0.797
2.062GluMet: 2.062 ± 0.361
1.819GluAsn: 1.819 ± 0.337
2.608GluPro: 2.608 ± 0.389
2.001GluGln: 2.001 ± 0.295
4.912GluArg: 4.912 ± 0.543
3.336GluSer: 3.336 ± 0.386
4.003GluThr: 4.003 ± 0.442
4.185GluVal: 4.185 ± 0.522
1.395GluTrp: 1.395 ± 0.288
2.365GluTyr: 2.365 ± 0.385
0.0GluXaa: 0.0 ± 0.0
Phe
3.032PheAla: 3.032 ± 0.369
0.243PheCys: 0.243 ± 0.138
2.244PheAsp: 2.244 ± 0.338
2.365PheGlu: 2.365 ± 0.356
0.849PhePhe: 0.849 ± 0.267
3.517PheGly: 3.517 ± 0.477
0.849PheHis: 0.849 ± 0.28
1.88PheIle: 1.88 ± 0.301
1.152PheLys: 1.152 ± 0.269
2.608PheLeu: 2.608 ± 0.515
0.606PheMet: 0.606 ± 0.162
1.819PheAsn: 1.819 ± 0.348
2.001PhePro: 2.001 ± 0.32
1.092PheGln: 1.092 ± 0.292
2.123PheArg: 2.123 ± 0.358
1.88PheSer: 1.88 ± 0.345
2.365PheThr: 2.365 ± 0.302
2.426PheVal: 2.426 ± 0.404
0.303PheTrp: 0.303 ± 0.125
0.606PheTyr: 0.606 ± 0.173
0.0PheXaa: 0.0 ± 0.0
Gly
6.61GlyAla: 6.61 ± 0.916
0.728GlyCys: 0.728 ± 0.21
6.125GlyAsp: 6.125 ± 0.562
4.245GlyGlu: 4.245 ± 0.628
3.093GlyPhe: 3.093 ± 0.495
7.581GlyGly: 7.581 ± 1.245
1.759GlyHis: 1.759 ± 0.376
3.821GlyIle: 3.821 ± 0.566
3.881GlyLys: 3.881 ± 0.55
6.247GlyLeu: 6.247 ± 0.787
2.608GlyMet: 2.608 ± 0.441
3.457GlyAsn: 3.457 ± 0.414
3.275GlyPro: 3.275 ± 0.451
3.881GlyGln: 3.881 ± 0.419
3.699GlyArg: 3.699 ± 0.456
3.942GlySer: 3.942 ± 0.524
5.216GlyThr: 5.216 ± 0.867
6.732GlyVal: 6.732 ± 0.66
1.516GlyTrp: 1.516 ± 0.269
2.426GlyTyr: 2.426 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
1.516HisAla: 1.516 ± 0.299
0.243HisCys: 0.243 ± 0.121
1.334HisAsp: 1.334 ± 0.308
1.031HisGlu: 1.031 ± 0.225
0.667HisPhe: 0.667 ± 0.196
1.516HisGly: 1.516 ± 0.398
0.606HisHis: 0.606 ± 0.18
1.152HisIle: 1.152 ± 0.25
0.849HisLys: 0.849 ± 0.249
1.274HisLeu: 1.274 ± 0.321
0.425HisMet: 0.425 ± 0.149
0.425HisAsn: 0.425 ± 0.175
1.274HisPro: 1.274 ± 0.24
0.667HisGln: 0.667 ± 0.239
1.759HisArg: 1.759 ± 0.357
0.97HisSer: 0.97 ± 0.268
1.031HisThr: 1.031 ± 0.25
1.395HisVal: 1.395 ± 0.291
0.303HisTrp: 0.303 ± 0.15
0.728HisTyr: 0.728 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
6.125IleAla: 6.125 ± 0.606
0.485IleCys: 0.485 ± 0.144
3.881IleAsp: 3.881 ± 0.464
4.063IleGlu: 4.063 ± 0.453
1.213IlePhe: 1.213 ± 0.233
4.73IleGly: 4.73 ± 0.554
1.031IleHis: 1.031 ± 0.214
1.334IleIle: 1.334 ± 0.28
2.305IleLys: 2.305 ± 0.389
3.76IleLeu: 3.76 ± 0.417
0.485IleMet: 0.485 ± 0.236
1.88IleAsn: 1.88 ± 0.376
3.517IlePro: 3.517 ± 0.528
1.577IleGln: 1.577 ± 0.316
2.79IleArg: 2.79 ± 0.391
2.062IleSer: 2.062 ± 0.483
2.79IleThr: 2.79 ± 0.34
3.032IleVal: 3.032 ± 0.496
0.303IleTrp: 0.303 ± 0.119
1.274IleTyr: 1.274 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
5.398LysAla: 5.398 ± 0.805
0.303LysCys: 0.303 ± 0.159
2.426LysAsp: 2.426 ± 0.327
2.729LysGlu: 2.729 ± 0.373
0.91LysPhe: 0.91 ± 0.211
3.154LysGly: 3.154 ± 0.41
0.728LysHis: 0.728 ± 0.267
2.547LysIle: 2.547 ± 0.336
2.911LysLys: 2.911 ± 0.513
3.942LysLeu: 3.942 ± 0.528
1.031LysMet: 1.031 ± 0.259
1.516LysAsn: 1.516 ± 0.236
2.365LysPro: 2.365 ± 0.438
1.516LysGln: 1.516 ± 0.291
3.214LysArg: 3.214 ± 0.481
2.001LysSer: 2.001 ± 0.298
3.396LysThr: 3.396 ± 0.493
4.124LysVal: 4.124 ± 0.519
0.728LysTrp: 0.728 ± 0.232
1.759LysTyr: 1.759 ± 0.303
0.0LysXaa: 0.0 ± 0.0
Leu
8.915LeuAla: 8.915 ± 0.973
0.606LeuCys: 0.606 ± 0.186
4.973LeuAsp: 4.973 ± 0.589
5.398LeuGlu: 5.398 ± 0.726
2.183LeuPhe: 2.183 ± 0.278
6.368LeuGly: 6.368 ± 0.746
1.759LeuHis: 1.759 ± 0.365
4.609LeuIle: 4.609 ± 0.409
3.275LeuLys: 3.275 ± 0.458
6.186LeuLeu: 6.186 ± 0.653
2.911LeuMet: 2.911 ± 0.53
2.426LeuAsn: 2.426 ± 0.4
4.367LeuPro: 4.367 ± 0.442
2.729LeuGln: 2.729 ± 0.674
6.732LeuArg: 6.732 ± 0.702
4.912LeuSer: 4.912 ± 0.631
6.307LeuThr: 6.307 ± 0.817
4.306LeuVal: 4.306 ± 0.665
1.395LeuTrp: 1.395 ± 0.282
2.244LeuTyr: 2.244 ± 0.414
0.0LeuXaa: 0.0 ± 0.0
Met
3.093MetAla: 3.093 ± 0.472
0.061MetCys: 0.061 ± 0.049
0.91MetAsp: 0.91 ± 0.191
1.819MetGlu: 1.819 ± 0.237
0.485MetPhe: 0.485 ± 0.146
1.577MetGly: 1.577 ± 0.318
0.546MetHis: 0.546 ± 0.178
1.577MetIle: 1.577 ± 0.293
1.88MetLys: 1.88 ± 0.287
1.577MetLeu: 1.577 ± 0.356
0.425MetMet: 0.425 ± 0.133
0.667MetAsn: 0.667 ± 0.195
1.456MetPro: 1.456 ± 0.35
1.031MetGln: 1.031 ± 0.244
1.456MetArg: 1.456 ± 0.276
2.001MetSer: 2.001 ± 0.301
2.305MetThr: 2.305 ± 0.309
1.395MetVal: 1.395 ± 0.295
0.243MetTrp: 0.243 ± 0.105
0.606MetTyr: 0.606 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
3.275AsnAla: 3.275 ± 0.493
0.546AsnCys: 0.546 ± 0.206
2.062AsnAsp: 2.062 ± 0.303
2.305AsnGlu: 2.305 ± 0.454
0.788AsnPhe: 0.788 ± 0.272
3.396AsnGly: 3.396 ± 0.516
0.849AsnHis: 0.849 ± 0.179
1.516AsnIle: 1.516 ± 0.281
1.274AsnLys: 1.274 ± 0.296
2.972AsnLeu: 2.972 ± 0.509
0.91AsnMet: 0.91 ± 0.247
0.728AsnAsn: 0.728 ± 0.228
2.668AsnPro: 2.668 ± 0.49
0.849AsnGln: 0.849 ± 0.266
2.001AsnArg: 2.001 ± 0.398
1.516AsnSer: 1.516 ± 0.293
1.516AsnThr: 1.516 ± 0.267
2.123AsnVal: 2.123 ± 0.309
0.728AsnTrp: 0.728 ± 0.223
0.91AsnTyr: 0.91 ± 0.24
0.0AsnXaa: 0.0 ± 0.0
Pro
5.761ProAla: 5.761 ± 0.605
0.425ProCys: 0.425 ± 0.176
3.821ProAsp: 3.821 ± 0.584
4.912ProGlu: 4.912 ± 0.594
2.123ProPhe: 2.123 ± 0.355
4.003ProGly: 4.003 ± 0.553
0.788ProHis: 0.788 ± 0.203
2.547ProIle: 2.547 ± 0.329
2.001ProLys: 2.001 ± 0.477
3.214ProLeu: 3.214 ± 0.462
1.274ProMet: 1.274 ± 0.251
2.244ProAsn: 2.244 ± 0.399
2.305ProPro: 2.305 ± 0.365
1.698ProGln: 1.698 ± 0.301
3.881ProArg: 3.881 ± 0.537
2.305ProSer: 2.305 ± 0.435
3.699ProThr: 3.699 ± 0.509
4.063ProVal: 4.063 ± 0.502
1.334ProTrp: 1.334 ± 0.354
1.213ProTyr: 1.213 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
4.973GlnAla: 4.973 ± 0.728
0.182GlnCys: 0.182 ± 0.104
1.213GlnAsp: 1.213 ± 0.223
1.698GlnGlu: 1.698 ± 0.328
1.456GlnPhe: 1.456 ± 0.31
3.214GlnGly: 3.214 ± 0.513
0.485GlnHis: 0.485 ± 0.148
2.244GlnIle: 2.244 ± 0.334
1.516GlnLys: 1.516 ± 0.35
3.578GlnLeu: 3.578 ± 0.714
1.092GlnMet: 1.092 ± 0.227
0.91GlnAsn: 0.91 ± 0.335
1.577GlnPro: 1.577 ± 0.268
2.608GlnGln: 2.608 ± 0.562
2.608GlnArg: 2.608 ± 0.461
1.88GlnSer: 1.88 ± 0.276
2.001GlnThr: 2.001 ± 0.38
2.547GlnVal: 2.547 ± 0.305
0.728GlnTrp: 0.728 ± 0.203
0.849GlnTyr: 0.849 ± 0.211
0.0GlnXaa: 0.0 ± 0.0
Arg
5.458ArgAla: 5.458 ± 0.635
0.728ArgCys: 0.728 ± 0.204
4.124ArgAsp: 4.124 ± 0.539
5.519ArgGlu: 5.519 ± 0.706
2.608ArgPhe: 2.608 ± 0.45
4.609ArgGly: 4.609 ± 0.582
1.456ArgHis: 1.456 ± 0.315
3.457ArgIle: 3.457 ± 0.499
3.214ArgLys: 3.214 ± 0.473
5.701ArgLeu: 5.701 ± 0.609
2.062ArgMet: 2.062 ± 0.314
2.001ArgAsn: 2.001 ± 0.316
2.365ArgPro: 2.365 ± 0.352
2.062ArgGln: 2.062 ± 0.326
4.973ArgArg: 4.973 ± 0.692
2.911ArgSer: 2.911 ± 0.48
3.396ArgThr: 3.396 ± 0.473
4.245ArgVal: 4.245 ± 0.539
1.516ArgTrp: 1.516 ± 0.332
2.183ArgTyr: 2.183 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
5.398SerAla: 5.398 ± 0.568
0.606SerCys: 0.606 ± 0.221
3.336SerAsp: 3.336 ± 0.495
3.214SerGlu: 3.214 ± 0.498
2.062SerPhe: 2.062 ± 0.379
4.003SerGly: 4.003 ± 0.556
0.485SerHis: 0.485 ± 0.213
1.819SerIle: 1.819 ± 0.351
2.547SerLys: 2.547 ± 0.476
4.185SerLeu: 4.185 ± 0.553
1.577SerMet: 1.577 ± 0.281
1.577SerAsn: 1.577 ± 0.506
3.032SerPro: 3.032 ± 0.428
2.062SerGln: 2.062 ± 0.371
3.093SerArg: 3.093 ± 0.408
2.729SerSer: 2.729 ± 0.353
3.214SerThr: 3.214 ± 0.494
3.154SerVal: 3.154 ± 0.502
1.334SerTrp: 1.334 ± 0.308
1.395SerTyr: 1.395 ± 0.304
0.0SerXaa: 0.0 ± 0.0
Thr
6.429ThrAla: 6.429 ± 0.672
0.606ThrCys: 0.606 ± 0.198
3.457ThrAsp: 3.457 ± 0.561
3.639ThrGlu: 3.639 ± 0.5
2.001ThrPhe: 2.001 ± 0.383
5.883ThrGly: 5.883 ± 0.797
0.788ThrHis: 0.788 ± 0.261
2.487ThrIle: 2.487 ± 0.378
3.214ThrLys: 3.214 ± 0.44
5.337ThrLeu: 5.337 ± 0.713
1.152ThrMet: 1.152 ± 0.26
2.123ThrAsn: 2.123 ± 0.365
4.67ThrPro: 4.67 ± 0.509
2.183ThrGln: 2.183 ± 0.306
3.032ThrArg: 3.032 ± 0.423
3.093ThrSer: 3.093 ± 0.546
3.821ThrThr: 3.821 ± 0.6
5.034ThrVal: 5.034 ± 0.613
1.213ThrTrp: 1.213 ± 0.253
1.819ThrTyr: 1.819 ± 0.331
0.0ThrXaa: 0.0 ± 0.0
Val
5.943ValAla: 5.943 ± 0.8
0.667ValCys: 0.667 ± 0.214
5.155ValAsp: 5.155 ± 0.535
4.367ValGlu: 4.367 ± 0.542
2.123ValPhe: 2.123 ± 0.483
5.155ValGly: 5.155 ± 0.533
1.031ValHis: 1.031 ± 0.255
4.185ValIle: 4.185 ± 0.551
3.517ValLys: 3.517 ± 0.492
5.276ValLeu: 5.276 ± 0.618
0.91ValMet: 0.91 ± 0.3
2.729ValAsn: 2.729 ± 0.414
3.517ValPro: 3.517 ± 0.54
2.001ValGln: 2.001 ± 0.335
5.094ValArg: 5.094 ± 0.656
4.67ValSer: 4.67 ± 0.624
4.912ValThr: 4.912 ± 0.526
5.519ValVal: 5.519 ± 0.463
1.637ValTrp: 1.637 ± 0.342
1.88ValTyr: 1.88 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
1.698TrpAla: 1.698 ± 0.349
0.364TrpCys: 0.364 ± 0.149
1.092TrpAsp: 1.092 ± 0.287
1.274TrpGlu: 1.274 ± 0.256
0.788TrpPhe: 0.788 ± 0.227
1.213TrpGly: 1.213 ± 0.283
0.485TrpHis: 0.485 ± 0.175
0.728TrpIle: 0.728 ± 0.187
0.849TrpLys: 0.849 ± 0.23
1.395TrpLeu: 1.395 ± 0.299
0.485TrpMet: 0.485 ± 0.181
1.031TrpAsn: 1.031 ± 0.248
1.031TrpPro: 1.031 ± 0.252
1.092TrpGln: 1.092 ± 0.25
1.152TrpArg: 1.152 ± 0.24
1.031TrpSer: 1.031 ± 0.275
1.334TrpThr: 1.334 ± 0.307
1.092TrpVal: 1.092 ± 0.246
0.425TrpTrp: 0.425 ± 0.175
0.485TrpTyr: 0.485 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.032TyrAla: 3.032 ± 0.424
0.182TyrCys: 0.182 ± 0.12
1.819TyrAsp: 1.819 ± 0.318
2.123TyrGlu: 2.123 ± 0.379
0.91TyrPhe: 0.91 ± 0.211
2.365TyrGly: 2.365 ± 0.379
0.546TyrHis: 0.546 ± 0.191
1.152TyrIle: 1.152 ± 0.21
0.91TyrLys: 0.91 ± 0.238
2.547TyrLeu: 2.547 ± 0.388
0.485TyrMet: 0.485 ± 0.189
0.97TyrAsn: 0.97 ± 0.29
1.274TyrPro: 1.274 ± 0.258
1.334TyrGln: 1.334 ± 0.262
2.365TyrArg: 2.365 ± 0.435
1.577TyrSer: 1.577 ± 0.369
1.698TyrThr: 1.698 ± 0.341
1.759TyrVal: 1.759 ± 0.385
0.485TyrTrp: 0.485 ± 0.18
0.849TyrTyr: 0.849 ± 0.225
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski