Blautia hydrogenotrophica CAG:147

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; environmental samples

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3087 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5C6G8|R5C6G8_9FIRM ABC transporter domain-containing protein OS=Blautia hydrogenotrophica CAG:147 OX=1263061 GN=BN499_00718 PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 10.66SNLEE6 pKa = 3.96QEE8 pKa = 4.57LQRR11 pKa = 11.84QLRR14 pKa = 11.84QEE16 pKa = 3.94IEE18 pKa = 3.9IPEE21 pKa = 4.64CIQKK25 pKa = 10.33KK26 pKa = 10.07SKK28 pKa = 9.68QAYY31 pKa = 9.45LKK33 pKa = 10.18IKK35 pKa = 9.96SGQIRR40 pKa = 11.84QEE42 pKa = 4.16EE43 pKa = 4.56VLKK46 pKa = 10.24TPLGWMKK53 pKa = 9.54KK54 pKa = 6.47TAIALGSVAAALLLCMVLFLTNPVMAQDD82 pKa = 4.26FPVIGNLFSLLQDD95 pKa = 4.01KK96 pKa = 10.86ISFFGNFEE104 pKa = 4.67DD105 pKa = 4.66YY106 pKa = 8.43ATPLVPEE113 pKa = 4.68EE114 pKa = 4.28DD115 pKa = 3.46TSFSKK120 pKa = 10.32DD121 pKa = 3.13TDD123 pKa = 3.45SSGIFSKK130 pKa = 9.96TEE132 pKa = 4.05DD133 pKa = 3.83GLTITLSEE141 pKa = 4.69VYY143 pKa = 10.8ANTQAIYY150 pKa = 8.95LTVLMEE156 pKa = 4.27SEE158 pKa = 4.24EE159 pKa = 4.4AFPSTLIDD167 pKa = 3.58QQDD170 pKa = 3.6QPVLSLIASEE180 pKa = 5.27NYY182 pKa = 10.17DD183 pKa = 3.74FNPTPEE189 pKa = 4.46EE190 pKa = 4.19YY191 pKa = 10.93NNPIYY196 pKa = 10.28FNPEE200 pKa = 3.44GQFLDD205 pKa = 4.62DD206 pKa = 3.73YY207 pKa = 10.42TYY209 pKa = 11.53SCILRR214 pKa = 11.84LDD216 pKa = 3.64LRR218 pKa = 11.84EE219 pKa = 4.3ASLDD223 pKa = 3.34RR224 pKa = 11.84QAYY227 pKa = 8.01NEE229 pKa = 3.97AMIQAEE235 pKa = 4.27AAAQEE240 pKa = 3.95TDD242 pKa = 4.19AVINYY247 pKa = 9.11QNLQSFVEE255 pKa = 4.31IPEE258 pKa = 4.18QIHH261 pKa = 7.19LDD263 pKa = 3.7FQIQQVIGTLEE274 pKa = 4.01DD275 pKa = 3.16AQYY278 pKa = 10.21WDD280 pKa = 2.86SGYY283 pKa = 9.16TEE285 pKa = 4.69EE286 pKa = 5.27EE287 pKa = 4.06LTAMTDD293 pKa = 3.4EE294 pKa = 4.64EE295 pKa = 4.35FQEE298 pKa = 4.49VMKK301 pKa = 10.67QMPEE305 pKa = 4.03EE306 pKa = 4.31YY307 pKa = 9.64NQHH310 pKa = 6.0PNQYY314 pKa = 9.25EE315 pKa = 4.24NYY317 pKa = 8.58WFDD320 pKa = 4.03GPWDD324 pKa = 4.07FSLDD328 pKa = 3.61LTVDD332 pKa = 3.63SSRR335 pKa = 11.84VQTIEE340 pKa = 3.51INEE343 pKa = 3.95INEE346 pKa = 4.08NGVGISNVILTPYY359 pKa = 10.13EE360 pKa = 3.95LTVNDD365 pKa = 4.78AYY367 pKa = 10.99ADD369 pKa = 3.68GVPFYY374 pKa = 11.01DD375 pKa = 3.85YY376 pKa = 11.31FLVALDD382 pKa = 3.9ANGNKK387 pKa = 9.97LPYY390 pKa = 9.87NDD392 pKa = 4.95SNGNCNYY399 pKa = 8.66FTIQDD404 pKa = 3.71RR405 pKa = 11.84DD406 pKa = 3.24ISTVDD411 pKa = 4.7LYY413 pKa = 10.9ILDD416 pKa = 3.61YY417 pKa = 10.37TEE419 pKa = 5.77YY420 pKa = 9.57MDD422 pKa = 4.15EE423 pKa = 4.88LKK425 pKa = 11.23GEE427 pKa = 4.06EE428 pKa = 4.07NYY430 pKa = 11.13NNNEE434 pKa = 4.05NKK436 pKa = 10.1PEE438 pKa = 3.95EE439 pKa = 4.6EE440 pKa = 3.97KK441 pKa = 10.64WSTLLDD447 pKa = 3.76DD448 pKa = 3.7RR449 pKa = 11.84CFYY452 pKa = 10.13HH453 pKa = 5.69TTLNLTPVEE462 pKa = 4.17

Molecular weight: 53.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5C5M1|R5C5M1_9FIRM Uncharacterized protein OS=Blautia hydrogenotrophica CAG:147 OX=1263061 GN=BN499_02185 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3087

0

3087

947112

29

2797

306.8

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.315 ± 0.046

1.642 ± 0.019

5.117 ± 0.031

7.908 ± 0.05

4.018 ± 0.031

7.467 ± 0.043

1.717 ± 0.023

6.974 ± 0.038

6.578 ± 0.035

9.373 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.088 ± 0.023

3.907 ± 0.025

3.391 ± 0.024

3.645 ± 0.03

4.741 ± 0.037

5.742 ± 0.03

5.173 ± 0.032

7.259 ± 0.036

0.933 ± 0.016

3.999 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski