Amino acid dipepetide frequency for Actinia tenebrosa (Australian red waratah sea anemone)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.06AlaAla: 4.06 ± 0.027	1.149AlaCys: 1.149 ± 0.012	2.834AlaAsp: 2.834 ± 0.016	3.585AlaGlu: 3.585 ± 0.025	2.393AlaPhe: 2.393 ± 0.015	3.177AlaGly: 3.177 ± 0.025	1.138AlaHis: 1.138 ± 0.01	3.448AlaIle: 3.448 ± 0.022	4.011AlaLys: 4.011 ± 0.02	5.322AlaLeu: 5.322 ± 0.03	1.435AlaMet: 1.435 ± 0.011	2.493AlaAsn: 2.493 ± 0.016	2.39AlaPro: 2.39 ± 0.022	2.083AlaGln: 2.083 ± 0.015	2.866AlaArg: 2.866 ± 0.017	4.833AlaSer: 4.833 ± 0.024	3.449AlaThr: 3.449 ± 0.023	4.197AlaVal: 4.197 ± 0.022	0.635AlaTrp: 0.635 ± 0.008	1.61AlaTyr: 1.61 ± 0.013	0.073AlaXaa: 0.073 ± 0.002
Cys	1.098CysAla: 1.098 ± 0.012	0.626CysCys: 0.626 ± 0.011	1.208CysAsp: 1.208 ± 0.018	1.248CysGlu: 1.248 ± 0.017	0.899CysPhe: 0.899 ± 0.01	1.422CysGly: 1.422 ± 0.018	0.614CysHis: 0.614 ± 0.012	1.181CysIle: 1.181 ± 0.013	1.545CysLys: 1.545 ± 0.019	1.927CysLeu: 1.927 ± 0.017	0.449CysMet: 0.449 ± 0.007	1.091CysAsn: 1.091 ± 0.017	1.219CysPro: 1.219 ± 0.035	0.982CysGln: 0.982 ± 0.015	1.171CysArg: 1.171 ± 0.014	1.966CysSer: 1.966 ± 0.021	1.288CysThr: 1.288 ± 0.017	1.392CysVal: 1.392 ± 0.019	0.307CysTrp: 0.307 ± 0.01	0.737CysTyr: 0.737 ± 0.011	0.034CysXaa: 0.034 ± 0.002
Asp	2.945AspAla: 2.945 ± 0.02	1.137AspCys: 1.137 ± 0.017	4.168AspAsp: 4.168 ± 0.035	4.55AspGlu: 4.55 ± 0.032	2.239AspPhe: 2.239 ± 0.015	3.596AspGly: 3.596 ± 0.024	1.213AspHis: 1.213 ± 0.01	3.352AspIle: 3.352 ± 0.022	3.84AspLys: 3.84 ± 0.021	4.749AspLeu: 4.749 ± 0.028	1.21AspMet: 1.21 ± 0.01	2.706AspAsn: 2.706 ± 0.016	2.288AspPro: 2.288 ± 0.018	1.976AspGln: 1.976 ± 0.015	2.491AspArg: 2.491 ± 0.016	4.41AspSer: 4.41 ± 0.03	2.748AspThr: 2.748 ± 0.017	3.761AspVal: 3.761 ± 0.027	0.606AspTrp: 0.606 ± 0.008	1.689AspTyr: 1.689 ± 0.014	0.085AspXaa: 0.085 ± 0.003
Glu	3.878GluAla: 3.878 ± 0.026	1.345GluCys: 1.345 ± 0.018	4.435GluAsp: 4.435 ± 0.034	6.906GluGlu: 6.906 ± 0.081	2.208GluPhe: 2.208 ± 0.018	3.51GluGly: 3.51 ± 0.026	1.383GluHis: 1.383 ± 0.012	3.786GluIle: 3.786 ± 0.021	5.878GluLys: 5.878 ± 0.049	5.595GluLeu: 5.595 ± 0.042	1.67GluMet: 1.67 ± 0.014	3.84GluAsn: 3.84 ± 0.029	2.315GluPro: 2.315 ± 0.021	2.735GluGln: 2.735 ± 0.025	3.736GluArg: 3.736 ± 0.032	4.796GluSer: 4.796 ± 0.034	3.645GluThr: 3.645 ± 0.023	4.025GluVal: 4.025 ± 0.032	0.757GluTrp: 0.757 ± 0.014	1.904GluTyr: 1.904 ± 0.015	0.087GluXaa: 0.087 ± 0.003
Phe	2.121PheAla: 2.121 ± 0.014	0.941PheCys: 0.941 ± 0.01	2.156PheAsp: 2.156 ± 0.014	2.267PheGlu: 2.267 ± 0.015	1.695PhePhe: 1.695 ± 0.016	2.474PheGly: 2.474 ± 0.018	0.976PheHis: 0.976 ± 0.01	2.302PheIle: 2.302 ± 0.016	2.495PheLys: 2.495 ± 0.018	3.745PheLeu: 3.745 ± 0.026	0.821PheMet: 0.821 ± 0.009	1.794PheAsn: 1.794 ± 0.013	1.71PhePro: 1.71 ± 0.014	1.523PheGln: 1.523 ± 0.012	1.764PheArg: 1.764 ± 0.013	3.237PheSer: 3.237 ± 0.017	2.345PheThr: 2.345 ± 0.015	2.709PheVal: 2.709 ± 0.019	0.484PheTrp: 0.484 ± 0.006	1.423PheTyr: 1.423 ± 0.013	0.051PheXaa: 0.051 ± 0.002
Gly	2.998GlyAla: 2.998 ± 0.025	1.227GlyCys: 1.227 ± 0.015	3.001GlyAsp: 3.001 ± 0.022	3.35GlyGlu: 3.35 ± 0.028	2.552GlyPhe: 2.552 ± 0.019	3.938GlyGly: 3.938 ± 0.062	1.432GlyHis: 1.432 ± 0.014	3.28GlyIle: 3.28 ± 0.017	4.179GlyLys: 4.179 ± 0.024	4.51GlyLeu: 4.51 ± 0.024	1.322GlyMet: 1.322 ± 0.015	3.078GlyAsn: 3.078 ± 0.022	2.378GlyPro: 2.378 ± 0.039	2.31GlyGln: 2.31 ± 0.017	3.161GlyArg: 3.161 ± 0.026	5.068GlySer: 5.068 ± 0.041	3.374GlyThr: 3.374 ± 0.032	3.596GlyVal: 3.596 ± 0.018	0.776GlyTrp: 0.776 ± 0.012	2.151GlyTyr: 2.151 ± 0.022	0.077GlyXaa: 0.077 ± 0.003
His	1.15HisAla: 1.15 ± 0.011	0.586HisCys: 0.586 ± 0.007	1.161HisAsp: 1.161 ± 0.01	1.376HisGlu: 1.376 ± 0.013	1.01HisPhe: 1.01 ± 0.009	1.455HisGly: 1.455 ± 0.014	0.77HisHis: 0.77 ± 0.01	1.212HisIle: 1.212 ± 0.01	1.384HisLys: 1.384 ± 0.011	2.207HisLeu: 2.207 ± 0.015	0.501HisMet: 0.501 ± 0.007	0.983HisAsn: 0.983 ± 0.011	1.243HisPro: 1.243 ± 0.013	1.009HisGln: 1.009 ± 0.011	1.236HisArg: 1.236 ± 0.012	1.899HisSer: 1.899 ± 0.014	1.16HisThr: 1.16 ± 0.012	1.548HisVal: 1.548 ± 0.012	0.283HisTrp: 0.283 ± 0.004	0.829HisTyr: 0.829 ± 0.009	0.032HisXaa: 0.032 ± 0.002
Ile	3.381IleAla: 3.381 ± 0.021	1.214IleCys: 1.214 ± 0.012	3.071IleAsp: 3.071 ± 0.017	3.472IleGlu: 3.472 ± 0.024	2.257IlePhe: 2.257 ± 0.017	2.973IleGly: 2.973 ± 0.019	1.335IleHis: 1.335 ± 0.009	3.223IleIle: 3.223 ± 0.02	3.868IleLys: 3.868 ± 0.022	4.955IleLeu: 4.955 ± 0.027	1.145IleMet: 1.145 ± 0.01	2.646IleAsn: 2.646 ± 0.016	2.898IlePro: 2.898 ± 0.015	2.34IleGln: 2.34 ± 0.011	2.722IleArg: 2.722 ± 0.015	4.46IleSer: 4.46 ± 0.019	3.444IleThr: 3.444 ± 0.021	3.462IleVal: 3.462 ± 0.02	0.612IleTrp: 0.612 ± 0.008	1.652IleTyr: 1.652 ± 0.013	0.07IleXaa: 0.07 ± 0.002
Lys	4.173LysAla: 4.173 ± 0.022	1.59LysCys: 1.59 ± 0.022	4.389LysAsp: 4.389 ± 0.03	6.073LysGlu: 6.073 ± 0.046	2.157LysPhe: 2.157 ± 0.013	3.691LysGly: 3.691 ± 0.029	1.612LysHis: 1.612 ± 0.014	3.834LysIle: 3.834 ± 0.021	6.181LysLys: 6.181 ± 0.044	5.665LysLeu: 5.665 ± 0.032	1.614LysMet: 1.614 ± 0.013	3.55LysAsn: 3.55 ± 0.02	3.456LysPro: 3.456 ± 0.036	2.965LysGln: 2.965 ± 0.021	4.118LysArg: 4.118 ± 0.024	5.106LysSer: 5.106 ± 0.027	4.495LysThr: 4.495 ± 0.022	4.07LysVal: 4.07 ± 0.02	0.775LysTrp: 0.775 ± 0.009	2.161LysTyr: 2.161 ± 0.015	0.103LysXaa: 0.103 ± 0.004
Leu	5.195LeuAla: 5.195 ± 0.027	1.824LeuCys: 1.824 ± 0.016	4.612LeuAsp: 4.612 ± 0.024	5.947LeuGlu: 5.947 ± 0.045	3.398LeuPhe: 3.398 ± 0.025	4.482LeuGly: 4.482 ± 0.025	2.166LeuHis: 2.166 ± 0.016	4.325LeuIle: 4.325 ± 0.023	6.36LeuLys: 6.36 ± 0.032	8.058LeuLeu: 8.058 ± 0.05	1.922LeuMet: 1.922 ± 0.013	4.066LeuAsn: 4.066 ± 0.023	4.271LeuPro: 4.271 ± 0.025	4.166LeuGln: 4.166 ± 0.03	4.67LeuArg: 4.67 ± 0.022	7.028LeuSer: 7.028 ± 0.029	4.861LeuThr: 4.861 ± 0.025	5.408LeuVal: 5.408 ± 0.025	0.911LeuTrp: 0.911 ± 0.009	2.538LeuTyr: 2.538 ± 0.02	0.11LeuXaa: 0.11 ± 0.004
Met	1.84MetAla: 1.84 ± 0.013	0.434MetCys: 0.434 ± 0.006	1.275MetAsp: 1.275 ± 0.011	1.671MetGlu: 1.671 ± 0.013	0.895MetPhe: 0.895 ± 0.01	1.068MetGly: 1.068 ± 0.011	0.417MetHis: 0.417 ± 0.005	1.141MetIle: 1.141 ± 0.01	1.728MetLys: 1.728 ± 0.012	1.809MetLeu: 1.809 ± 0.014	0.631MetMet: 0.631 ± 0.008	1.148MetAsn: 1.148 ± 0.011	0.973MetPro: 0.973 ± 0.01	0.859MetGln: 0.859 ± 0.008	1.086MetArg: 1.086 ± 0.011	1.794MetSer: 1.794 ± 0.014	1.355MetThr: 1.355 ± 0.012	1.339MetVal: 1.339 ± 0.011	0.24MetTrp: 0.24 ± 0.004	0.692MetTyr: 0.692 ± 0.008	0.027MetXaa: 0.027 ± 0.002
Asn	2.712AsnAla: 2.712 ± 0.016	1.113AsnCys: 1.113 ± 0.014	2.8AsnAsp: 2.8 ± 0.017	3.472AsnGlu: 3.472 ± 0.023	1.743AsnPhe: 1.743 ± 0.013	3.318AsnGly: 3.318 ± 0.029	1.108AsnHis: 1.108 ± 0.01	3.043AsnIle: 3.043 ± 0.017	3.427AsnLys: 3.427 ± 0.02	3.979AsnLeu: 3.979 ± 0.022	1.087AsnMet: 1.087 ± 0.01	2.622AsnAsn: 2.622 ± 0.018	2.372AsnPro: 2.372 ± 0.016	2.053AsnGln: 2.053 ± 0.018	2.213AsnArg: 2.213 ± 0.015	3.817AsnSer: 3.817 ± 0.021	2.94AsnThr: 2.94 ± 0.02	3.091AsnVal: 3.091 ± 0.017	0.517AsnTrp: 0.517 ± 0.007	1.499AsnTyr: 1.499 ± 0.012	0.074AsnXaa: 0.074 ± 0.003
Pro	2.561ProAla: 2.561 ± 0.018	0.983ProCys: 0.983 ± 0.021	2.462ProAsp: 2.462 ± 0.017	3.037ProGlu: 3.037 ± 0.031	1.728ProPhe: 1.728 ± 0.013	3.009ProGly: 3.009 ± 0.045	0.995ProHis: 0.995 ± 0.009	2.17ProIle: 2.17 ± 0.013	3.142ProLys: 3.142 ± 0.024	3.833ProLeu: 3.833 ± 0.019	0.929ProMet: 0.929 ± 0.011	2.193ProAsn: 2.193 ± 0.017	3.464ProPro: 3.464 ± 0.039	1.924ProGln: 1.924 ± 0.019	2.45ProArg: 2.45 ± 0.02	4.62ProSer: 4.62 ± 0.026	3.03ProThr: 3.03 ± 0.028	3.167ProVal: 3.167 ± 0.021	0.496ProTrp: 0.496 ± 0.007	1.409ProTyr: 1.409 ± 0.013	0.066ProXaa: 0.066 ± 0.003
Gln	2.492GlnAla: 2.492 ± 0.017	0.944GlnCys: 0.944 ± 0.023	2.266GlnAsp: 2.266 ± 0.017	3.245GlnGlu: 3.245 ± 0.03	1.342GlnPhe: 1.342 ± 0.011	2.43GlnGly: 2.43 ± 0.023	0.974GlnHis: 0.974 ± 0.01	2.08GlnIle: 2.08 ± 0.012	2.737GlnLys: 2.737 ± 0.018	3.463GlnLeu: 3.463 ± 0.026	0.991GlnMet: 0.991 ± 0.011	2.087GlnAsn: 2.087 ± 0.017	1.948GlnPro: 1.948 ± 0.019	2.548GlnGln: 2.548 ± 0.047	2.341GlnArg: 2.341 ± 0.018	3.056GlnSer: 3.056 ± 0.021	2.326GlnThr: 2.326 ± 0.016	2.443GlnVal: 2.443 ± 0.016	0.499GlnTrp: 0.499 ± 0.01	1.236GlnTyr: 1.236 ± 0.011	0.053GlnXaa: 0.053 ± 0.002
Arg	2.686ArgAla: 2.686 ± 0.019	1.147ArgCys: 1.147 ± 0.016	2.752ArgAsp: 2.752 ± 0.018	3.505ArgGlu: 3.505 ± 0.027	1.964ArgPhe: 1.964 ± 0.013	2.841ArgGly: 2.841 ± 0.022	1.322ArgHis: 1.322 ± 0.012	2.885ArgIle: 2.885 ± 0.017	4.169ArgLys: 4.169 ± 0.023	4.474ArgLeu: 4.474 ± 0.024	1.164ArgMet: 1.164 ± 0.011	2.608ArgAsn: 2.608 ± 0.018	2.378ArgPro: 2.378 ± 0.021	2.33ArgGln: 2.33 ± 0.019	3.762ArgArg: 3.762 ± 0.044	4.033ArgSer: 4.033 ± 0.027	2.835ArgThr: 2.835 ± 0.018	2.954ArgVal: 2.954 ± 0.017	0.613ArgTrp: 0.613 ± 0.008	1.745ArgTyr: 1.745 ± 0.014	0.065ArgXaa: 0.065 ± 0.002
Ser	4.318SerAla: 4.318 ± 0.023	1.876SerCys: 1.876 ± 0.018	4.411SerAsp: 4.411 ± 0.024	4.765SerGlu: 4.765 ± 0.028	3.382SerPhe: 3.382 ± 0.019	4.865SerGly: 4.865 ± 0.037	1.828SerHis: 1.828 ± 0.014	4.311SerIle: 4.311 ± 0.022	5.532SerLys: 5.532 ± 0.028	7.481SerLeu: 7.481 ± 0.031	1.766SerMet: 1.766 ± 0.014	4.043SerAsn: 4.043 ± 0.021	4.455SerPro: 4.455 ± 0.031	3.227SerGln: 3.227 ± 0.023	4.283SerArg: 4.283 ± 0.028	9.558SerSer: 9.558 ± 0.061	5.313SerThr: 5.313 ± 0.033	4.883SerVal: 4.883 ± 0.021	0.95SerTrp: 0.95 ± 0.011	2.502SerTyr: 2.502 ± 0.017	0.111SerXaa: 0.111 ± 0.004
Thr	3.701ThrAla: 3.701 ± 0.023	1.532ThrCys: 1.532 ± 0.021	2.973ThrAsp: 2.973 ± 0.021	3.502ThrGlu: 3.502 ± 0.023	2.324ThrPhe: 2.324 ± 0.015	3.503ThrGly: 3.503 ± 0.03	1.165ThrHis: 1.165 ± 0.012	3.279ThrIle: 3.279 ± 0.023	3.976ThrLys: 3.976 ± 0.019	4.905ThrLeu: 4.905 ± 0.022	1.244ThrMet: 1.244 ± 0.012	2.864ThrAsn: 2.864 ± 0.016	3.21ThrPro: 3.21 ± 0.023	2.139ThrGln: 2.139 ± 0.016	2.849ThrArg: 2.849 ± 0.018	5.685ThrSer: 5.685 ± 0.034	4.283ThrThr: 4.283 ± 0.041	3.828ThrVal: 3.828 ± 0.024	0.73ThrTrp: 0.73 ± 0.009	1.68ThrTyr: 1.68 ± 0.014	0.078ThrXaa: 0.078 ± 0.003
Val	3.699ValAla: 3.699 ± 0.019	1.545ValCys: 1.545 ± 0.02	3.386ValAsp: 3.386 ± 0.019	3.935ValGlu: 3.935 ± 0.032	2.838ValPhe: 2.838 ± 0.016	3.214ValGly: 3.214 ± 0.018	1.415ValHis: 1.415 ± 0.011	3.868ValIle: 3.868 ± 0.022	4.254ValLys: 4.254 ± 0.023	5.712ValLeu: 5.712 ± 0.028	1.47ValMet: 1.47 ± 0.012	3.029ValAsn: 3.029 ± 0.019	2.856ValPro: 2.856 ± 0.02	2.538ValGln: 2.538 ± 0.018	2.947ValArg: 2.947 ± 0.02	4.889ValSer: 4.889 ± 0.024	4.043ValThr: 4.043 ± 0.025	4.493ValVal: 4.493 ± 0.025	0.693ValTrp: 0.693 ± 0.008	2.015ValTyr: 2.015 ± 0.015	0.083ValXaa: 0.083 ± 0.003
Trp	0.526TrpAla: 0.526 ± 0.007	0.293TrpCys: 0.293 ± 0.011	0.603TrpAsp: 0.603 ± 0.009	0.605TrpGlu: 0.605 ± 0.007	0.521TrpPhe: 0.521 ± 0.008	0.618TrpGly: 0.618 ± 0.011	0.274TrpHis: 0.274 ± 0.005	0.713TrpIle: 0.713 ± 0.009	0.916TrpLys: 0.916 ± 0.009	1.057TrpLeu: 1.057 ± 0.011	0.33TrpMet: 0.33 ± 0.005	0.616TrpAsn: 0.616 ± 0.007	0.403TrpPro: 0.403 ± 0.007	0.426TrpGln: 0.426 ± 0.006	0.625TrpArg: 0.625 ± 0.007	0.979TrpSer: 0.979 ± 0.015	0.718TrpThr: 0.718 ± 0.009	0.615TrpVal: 0.615 ± 0.008	0.179TrpTrp: 0.179 ± 0.004	0.459TrpTyr: 0.459 ± 0.009	0.014TrpXaa: 0.014 ± 0.001
Tyr	1.584TyrAla: 1.584 ± 0.013	0.856TyrCys: 0.856 ± 0.019	1.725TyrAsp: 1.725 ± 0.012	1.829TyrGlu: 1.829 ± 0.015	1.432TyrPhe: 1.432 ± 0.013	2.097TyrGly: 2.097 ± 0.019	0.871TyrHis: 0.871 ± 0.01	1.698TyrIle: 1.698 ± 0.013	2.051TyrLys: 2.051 ± 0.022	2.763TyrLeu: 2.763 ± 0.02	0.693TyrMet: 0.693 ± 0.009	1.468TyrAsn: 1.468 ± 0.014	1.381TyrPro: 1.381 ± 0.014	1.316TyrGln: 1.316 ± 0.011	1.673TyrArg: 1.673 ± 0.014	2.509TyrSer: 2.509 ± 0.015	1.714TyrThr: 1.714 ± 0.016	1.859TyrVal: 1.859 ± 0.015	0.41TyrTrp: 0.41 ± 0.007	1.2TyrTyr: 1.2 ± 0.013	0.042TyrXaa: 0.042 ± 0.002
Xaa	0.067XaaAla: 0.067 ± 0.003	0.036XaaCys: 0.036 ± 0.002	0.073XaaAsp: 0.073 ± 0.003	0.094XaaGlu: 0.094 ± 0.003	0.058XaaPhe: 0.058 ± 0.002	0.076XaaGly: 0.076 ± 0.003	0.029XaaHis: 0.029 ± 0.002	0.07XaaIle: 0.07 ± 0.003	0.105XaaLys: 0.105 ± 0.003	0.107XaaLeu: 0.107 ± 0.004	0.028XaaMet: 0.028 ± 0.001	0.072XaaAsn: 0.072 ± 0.003	0.06XaaPro: 0.06 ± 0.003	0.056XaaGln: 0.056 ± 0.002	0.067XaaArg: 0.067 ± 0.002	0.116XaaSer: 0.116 ± 0.004	0.08XaaThr: 0.08 ± 0.003	0.081XaaVal: 0.081 ± 0.003	0.016XaaTrp: 0.016 ± 0.001	0.041XaaTyr: 0.041 ± 0.002	0.049XaaXaa: 0.049 ± 0.004

Statistics based on 23827 proteins (14063056 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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