Amino acid dipepetide frequency for Alkalibacterium sp. AK22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.25AlaAla: 6.25 ± 0.139
0.517AlaCys: 0.517 ± 0.03
4.424AlaAsp: 4.424 ± 0.089
5.602AlaGlu: 5.602 ± 0.13
3.757AlaPhe: 3.757 ± 0.087
5.899AlaGly: 5.899 ± 0.109
1.504AlaHis: 1.504 ± 0.052
5.282AlaIle: 5.282 ± 0.101
3.742AlaLys: 3.742 ± 0.081
7.918AlaLeu: 7.918 ± 0.108
2.046AlaMet: 2.046 ± 0.06
2.442AlaAsn: 2.442 ± 0.061
1.959AlaPro: 1.959 ± 0.058
2.688AlaGln: 2.688 ± 0.068
3.284AlaArg: 3.284 ± 0.072
4.615AlaSer: 4.615 ± 0.077
3.505AlaThr: 3.505 ± 0.077
6.343AlaVal: 6.343 ± 0.091
0.543AlaTrp: 0.543 ± 0.026
2.717AlaTyr: 2.717 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.305CysAla: 0.305 ± 0.023
0.064CysCys: 0.064 ± 0.011
0.292CysAsp: 0.292 ± 0.022
0.325CysGlu: 0.325 ± 0.024
0.234CysPhe: 0.234 ± 0.017
0.49CysGly: 0.49 ± 0.031
0.122CysHis: 0.122 ± 0.015
0.353CysIle: 0.353 ± 0.024
0.203CysLys: 0.203 ± 0.018
0.475CysLeu: 0.475 ± 0.028
0.116CysMet: 0.116 ± 0.012
0.2CysAsn: 0.2 ± 0.019
0.266CysPro: 0.266 ± 0.023
0.211CysGln: 0.211 ± 0.02
0.299CysArg: 0.299 ± 0.019
0.395CysSer: 0.395 ± 0.026
0.272CysThr: 0.272 ± 0.023
0.347CysVal: 0.347 ± 0.022
0.035CysTrp: 0.035 ± 0.008
0.145CysTyr: 0.145 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.803AspAla: 3.803 ± 0.085
0.328AspCys: 0.328 ± 0.025
2.948AspAsp: 2.948 ± 0.085
4.717AspGlu: 4.717 ± 0.084
2.667AspPhe: 2.667 ± 0.069
3.697AspGly: 3.697 ± 0.087
1.285AspHis: 1.285 ± 0.055
4.456AspIle: 4.456 ± 0.083
3.555AspLys: 3.555 ± 0.082
5.726AspLeu: 5.726 ± 0.084
1.749AspMet: 1.749 ± 0.056
1.989AspAsn: 1.989 ± 0.064
2.33AspPro: 2.33 ± 0.06
2.662AspGln: 2.662 ± 0.066
2.879AspArg: 2.879 ± 0.063
3.423AspSer: 3.423 ± 0.071
3.233AspThr: 3.233 ± 0.072
3.774AspVal: 3.774 ± 0.077
0.656AspTrp: 0.656 ± 0.033
2.554AspTyr: 2.554 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
6.891GluAla: 6.891 ± 0.112
0.278GluCys: 0.278 ± 0.022
4.52GluAsp: 4.52 ± 0.095
7.92GluGlu: 7.92 ± 0.161
2.172GluPhe: 2.172 ± 0.065
4.703GluGly: 4.703 ± 0.09
1.369GluHis: 1.369 ± 0.042
4.683GluIle: 4.683 ± 0.088
5.508GluLys: 5.508 ± 0.1
7.45GluLeu: 7.45 ± 0.121
2.133GluMet: 2.133 ± 0.064
3.103GluAsn: 3.103 ± 0.064
2.258GluPro: 2.258 ± 0.061
3.54GluGln: 3.54 ± 0.088
3.598GluArg: 3.598 ± 0.093
4.067GluSer: 4.067 ± 0.08
4.516GluThr: 4.516 ± 0.093
5.209GluVal: 5.209 ± 0.093
0.754GluTrp: 0.754 ± 0.035
2.041GluTyr: 2.041 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
2.859PheAla: 2.859 ± 0.065
0.217PheCys: 0.217 ± 0.018
2.879PheAsp: 2.879 ± 0.057
3.07PheGlu: 3.07 ± 0.071
2.255PhePhe: 2.255 ± 0.082
3.267PheGly: 3.267 ± 0.079
0.713PheHis: 0.713 ± 0.032
3.247PheIle: 3.247 ± 0.087
2.317PheLys: 2.317 ± 0.062
4.134PheLeu: 4.134 ± 0.107
1.2PheMet: 1.2 ± 0.044
1.919PheAsn: 1.919 ± 0.058
1.433PhePro: 1.433 ± 0.05
1.435PheGln: 1.435 ± 0.047
1.627PheArg: 1.627 ± 0.051
3.453PheSer: 3.453 ± 0.073
2.574PheThr: 2.574 ± 0.062
3.056PheVal: 3.056 ± 0.074
0.408PheTrp: 0.408 ± 0.026
1.658PheTyr: 1.658 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.915GlyAla: 4.915 ± 0.094
0.414GlyCys: 0.414 ± 0.026
3.433GlyAsp: 3.433 ± 0.079
4.688GlyGlu: 4.688 ± 0.087
3.322GlyPhe: 3.322 ± 0.079
4.984GlyGly: 4.984 ± 0.145
1.469GlyHis: 1.469 ± 0.055
5.463GlyIle: 5.463 ± 0.091
4.23GlyLys: 4.23 ± 0.099
7.121GlyLeu: 7.121 ± 0.122
2.184GlyMet: 2.184 ± 0.058
2.475GlyAsn: 2.475 ± 0.065
1.751GlyPro: 1.751 ± 0.055
2.548GlyGln: 2.548 ± 0.069
3.024GlyArg: 3.024 ± 0.079
4.16GlySer: 4.16 ± 0.092
4.018GlyThr: 4.018 ± 0.082
5.146GlyVal: 5.146 ± 0.089
0.702GlyTrp: 0.702 ± 0.035
2.63GlyTyr: 2.63 ± 0.065
0.0GlyXaa: 0.0 ± 0.0
His
1.446HisAla: 1.446 ± 0.05
0.145HisCys: 0.145 ± 0.017
1.069HisAsp: 1.069 ± 0.05
1.209HisGlu: 1.209 ± 0.041
0.966HisPhe: 0.966 ± 0.039
1.278HisGly: 1.278 ± 0.051
0.569HisHis: 0.569 ± 0.034
1.38HisIle: 1.38 ± 0.046
0.994HisLys: 0.994 ± 0.043
2.039HisLeu: 2.039 ± 0.054
0.533HisMet: 0.533 ± 0.027
0.714HisAsn: 0.714 ± 0.029
1.116HisPro: 1.116 ± 0.045
0.855HisGln: 0.855 ± 0.038
0.901HisArg: 0.901 ± 0.045
1.214HisSer: 1.214 ± 0.044
1.177HisThr: 1.177 ± 0.045
1.353HisVal: 1.353 ± 0.04
0.192HisTrp: 0.192 ± 0.018
0.957HisTyr: 0.957 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.515IleAla: 5.515 ± 0.102
0.403IleCys: 0.403 ± 0.023
4.737IleAsp: 4.737 ± 0.082
5.47IleGlu: 5.47 ± 0.091
2.771IlePhe: 2.771 ± 0.087
5.413IleGly: 5.413 ± 0.108
1.284IleHis: 1.284 ± 0.042
4.917IleIle: 4.917 ± 0.097
3.821IleLys: 3.821 ± 0.085
6.547IleLeu: 6.547 ± 0.139
1.763IleMet: 1.763 ± 0.057
2.763IleAsn: 2.763 ± 0.07
2.902IlePro: 2.902 ± 0.067
2.762IleGln: 2.762 ± 0.066
3.473IleArg: 3.473 ± 0.073
4.467IleSer: 4.467 ± 0.094
3.778IleThr: 3.778 ± 0.084
4.854IleVal: 4.854 ± 0.093
0.553IleTrp: 0.553 ± 0.032
2.113IleTyr: 2.113 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
4.986LysAla: 4.986 ± 0.094
0.203LysCys: 0.203 ± 0.021
3.786LysAsp: 3.786 ± 0.09
6.132LysGlu: 6.132 ± 0.111
1.269LysPhe: 1.269 ± 0.04
3.969LysGly: 3.969 ± 0.074
1.172LysHis: 1.172 ± 0.053
3.448LysIle: 3.448 ± 0.08
5.083LysLys: 5.083 ± 0.099
5.144LysLeu: 5.144 ± 0.106
1.716LysMet: 1.716 ± 0.051
2.688LysAsn: 2.688 ± 0.069
1.876LysPro: 1.876 ± 0.059
3.021LysGln: 3.021 ± 0.082
3.041LysArg: 3.041 ± 0.076
2.974LysSer: 2.974 ± 0.066
3.43LysThr: 3.43 ± 0.074
4.056LysVal: 4.056 ± 0.078
0.634LysTrp: 0.634 ± 0.036
1.684LysTyr: 1.684 ± 0.05
0.0LysXaa: 0.0 ± 0.0
Leu
7.324LeuAla: 7.324 ± 0.116
0.449LeuCys: 0.449 ± 0.027
5.784LeuAsp: 5.784 ± 0.092
6.847LeuGlu: 6.847 ± 0.109
4.941LeuPhe: 4.941 ± 0.116
6.057LeuGly: 6.057 ± 0.111
1.792LeuHis: 1.792 ± 0.054
7.445LeuIle: 7.445 ± 0.13
6.537LeuLys: 6.537 ± 0.117
10.306LeuLeu: 10.306 ± 0.192
2.768LeuMet: 2.768 ± 0.064
4.706LeuAsn: 4.706 ± 0.084
3.867LeuPro: 3.867 ± 0.085
3.056LeuGln: 3.056 ± 0.068
3.539LeuArg: 3.539 ± 0.074
7.856LeuSer: 7.856 ± 0.119
6.302LeuThr: 6.302 ± 0.124
6.436LeuVal: 6.436 ± 0.109
0.664LeuTrp: 0.664 ± 0.03
3.279LeuTyr: 3.279 ± 0.089
0.0LeuXaa: 0.0 ± 0.0
Met
2.104MetAla: 2.104 ± 0.058
0.084MetCys: 0.084 ± 0.012
1.794MetAsp: 1.794 ± 0.053
1.914MetGlu: 1.914 ± 0.058
0.934MetPhe: 0.934 ± 0.039
1.917MetGly: 1.917 ± 0.058
0.557MetHis: 0.557 ± 0.03
2.105MetIle: 2.105 ± 0.061
2.0MetLys: 2.0 ± 0.057
2.467MetLeu: 2.467 ± 0.068
0.832MetMet: 0.832 ± 0.037
1.491MetAsn: 1.491 ± 0.048
1.014MetPro: 1.014 ± 0.044
1.114MetGln: 1.114 ± 0.048
1.146MetArg: 1.146 ± 0.044
1.734MetSer: 1.734 ± 0.05
1.873MetThr: 1.873 ± 0.049
1.748MetVal: 1.748 ± 0.049
0.157MetTrp: 0.157 ± 0.015
0.702MetTyr: 0.702 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.708AsnAla: 2.708 ± 0.064
0.194AsnCys: 0.194 ± 0.018
2.166AsnAsp: 2.166 ± 0.056
3.085AsnGlu: 3.085 ± 0.074
1.398AsnPhe: 1.398 ± 0.044
2.977AsnGly: 2.977 ± 0.064
0.914AsnHis: 0.914 ± 0.043
2.781AsnIle: 2.781 ± 0.072
2.587AsnLys: 2.587 ± 0.066
3.615AsnLeu: 3.615 ± 0.067
1.139AsnMet: 1.139 ± 0.039
1.641AsnAsn: 1.641 ± 0.059
1.81AsnPro: 1.81 ± 0.054
1.962AsnGln: 1.962 ± 0.057
2.279AsnArg: 2.279 ± 0.059
1.911AsnSer: 1.911 ± 0.061
2.014AsnThr: 2.014 ± 0.059
2.583AsnVal: 2.583 ± 0.059
0.539AsnTrp: 0.539 ± 0.03
1.502AsnTyr: 1.502 ± 0.053
0.0AsnXaa: 0.0 ± 0.0
Pro
2.557ProAla: 2.557 ± 0.059
0.176ProCys: 0.176 ± 0.017
2.417ProAsp: 2.417 ± 0.067
3.148ProGlu: 3.148 ± 0.07
1.882ProPhe: 1.882 ± 0.062
2.183ProGly: 2.183 ± 0.061
0.746ProHis: 0.746 ± 0.034
2.217ProIle: 2.217 ± 0.065
1.833ProLys: 1.833 ± 0.063
3.455ProLeu: 3.455 ± 0.074
0.809ProMet: 0.809 ± 0.035
1.485ProAsn: 1.485 ± 0.05
0.707ProPro: 0.707 ± 0.031
1.131ProGln: 1.131 ± 0.035
1.125ProArg: 1.125 ± 0.039
2.217ProSer: 2.217 ± 0.062
1.888ProThr: 1.888 ± 0.056
3.134ProVal: 3.134 ± 0.073
0.296ProTrp: 0.296 ± 0.023
1.397ProTyr: 1.397 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
3.665GlnAla: 3.665 ± 0.092
0.143GlnCys: 0.143 ± 0.014
1.965GlnAsp: 1.965 ± 0.059
2.855GlnGlu: 2.855 ± 0.078
1.627GlnPhe: 1.627 ± 0.053
2.223GlnGly: 2.223 ± 0.052
0.696GlnHis: 0.696 ± 0.039
2.54GlnIle: 2.54 ± 0.07
2.523GlnLys: 2.523 ± 0.072
4.464GlnLeu: 4.464 ± 0.098
1.157GlnMet: 1.157 ± 0.048
1.523GlnAsn: 1.523 ± 0.052
1.392GlnPro: 1.392 ± 0.048
1.905GlnGln: 1.905 ± 0.071
1.571GlnArg: 1.571 ± 0.059
2.6GlnSer: 2.6 ± 0.067
2.765GlnThr: 2.765 ± 0.066
2.708GlnVal: 2.708 ± 0.062
0.411GlnTrp: 0.411 ± 0.026
1.195GlnTyr: 1.195 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
2.826ArgAla: 2.826 ± 0.075
0.203ArgCys: 0.203 ± 0.019
2.328ArgAsp: 2.328 ± 0.055
3.407ArgGlu: 3.407 ± 0.079
2.093ArgPhe: 2.093 ± 0.058
2.574ArgGly: 2.574 ± 0.069
1.023ArgHis: 1.023 ± 0.044
3.125ArgIle: 3.125 ± 0.074
3.062ArgLys: 3.062 ± 0.062
4.77ArgLeu: 4.77 ± 0.094
1.398ArgMet: 1.398 ± 0.046
1.759ArgAsn: 1.759 ± 0.044
1.539ArgPro: 1.539 ± 0.053
2.334ArgGln: 2.334 ± 0.071
2.267ArgArg: 2.267 ± 0.071
2.568ArgSer: 2.568 ± 0.056
2.287ArgThr: 2.287 ± 0.061
2.894ArgVal: 2.894 ± 0.059
0.418ArgTrp: 0.418 ± 0.028
1.685ArgTyr: 1.685 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
4.345SerAla: 4.345 ± 0.085
0.311SerCys: 0.311 ± 0.026
3.548SerAsp: 3.548 ± 0.079
4.279SerGlu: 4.279 ± 0.082
3.151SerPhe: 3.151 ± 0.068
4.894SerGly: 4.894 ± 0.088
1.255SerHis: 1.255 ± 0.048
4.534SerIle: 4.534 ± 0.093
3.545SerLys: 3.545 ± 0.071
6.563SerLeu: 6.563 ± 0.099
1.728SerMet: 1.728 ± 0.048
2.371SerAsn: 2.371 ± 0.067
2.116SerPro: 2.116 ± 0.052
2.638SerGln: 2.638 ± 0.073
3.103SerArg: 3.103 ± 0.071
4.349SerSer: 4.349 ± 0.102
3.297SerThr: 3.297 ± 0.074
4.803SerVal: 4.803 ± 0.096
0.614SerTrp: 0.614 ± 0.034
2.082SerTyr: 2.082 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
4.624ThrAla: 4.624 ± 0.089
0.295ThrCys: 0.295 ± 0.022
3.462ThrAsp: 3.462 ± 0.071
3.986ThrGlu: 3.986 ± 0.083
2.676ThrPhe: 2.676 ± 0.063
4.519ThrGly: 4.519 ± 0.088
1.279ThrHis: 1.279 ± 0.036
4.041ThrIle: 4.041 ± 0.079
2.525ThrLys: 2.525 ± 0.069
5.966ThrLeu: 5.966 ± 0.103
1.236ThrMet: 1.236 ± 0.046
2.009ThrAsn: 2.009 ± 0.053
2.313ThrPro: 2.313 ± 0.065
1.96ThrGln: 1.96 ± 0.056
2.232ThrArg: 2.232 ± 0.055
3.23ThrSer: 3.23 ± 0.069
2.92ThrThr: 2.92 ± 0.075
5.03ThrVal: 5.03 ± 0.102
0.446ThrTrp: 0.446 ± 0.029
2.024ThrTyr: 2.024 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
4.865ValAla: 4.865 ± 0.092
0.437ValCys: 0.437 ± 0.025
4.314ValAsp: 4.314 ± 0.093
5.146ValGlu: 5.146 ± 0.103
3.372ValPhe: 3.372 ± 0.071
4.599ValGly: 4.599 ± 0.096
1.412ValHis: 1.412 ± 0.048
5.317ValIle: 5.317 ± 0.092
4.006ValLys: 4.006 ± 0.075
7.23ValLeu: 7.23 ± 0.123
2.085ValMet: 2.085 ± 0.057
2.794ValAsn: 2.794 ± 0.068
2.478ValPro: 2.478 ± 0.06
2.352ValGln: 2.352 ± 0.055
3.111ValArg: 3.111 ± 0.058
5.395ValSer: 5.395 ± 0.085
4.262ValThr: 4.262 ± 0.08
4.897ValVal: 4.897 ± 0.102
0.598ValTrp: 0.598 ± 0.035
2.394ValTyr: 2.394 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.545TrpAla: 0.545 ± 0.028
0.046TrpCys: 0.046 ± 0.009
0.496TrpAsp: 0.496 ± 0.029
0.601TrpGlu: 0.601 ± 0.028
0.434TrpPhe: 0.434 ± 0.028
0.553TrpGly: 0.553 ± 0.032
0.195TrpHis: 0.195 ± 0.02
0.748TrpIle: 0.748 ± 0.035
0.504TrpLys: 0.504 ± 0.029
1.09TrpLeu: 1.09 ± 0.041
0.284TrpMet: 0.284 ± 0.021
0.423TrpAsn: 0.423 ± 0.031
0.241TrpPro: 0.241 ± 0.02
0.36TrpGln: 0.36 ± 0.02
0.397TrpArg: 0.397 ± 0.022
0.576TrpSer: 0.576 ± 0.032
0.556TrpThr: 0.556 ± 0.028
0.58TrpVal: 0.58 ± 0.031
0.111TrpTrp: 0.111 ± 0.014
0.293TrpTyr: 0.293 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.504TyrAla: 2.504 ± 0.07
0.241TyrCys: 0.241 ± 0.021
2.072TyrAsp: 2.072 ± 0.057
2.311TyrGlu: 2.311 ± 0.058
1.8TyrPhe: 1.8 ± 0.05
2.482TyrGly: 2.482 ± 0.054
0.748TyrHis: 0.748 ± 0.034
2.224TyrIle: 2.224 ± 0.052
1.737TyrLys: 1.737 ± 0.057
3.474TyrLeu: 3.474 ± 0.073
0.811TyrMet: 0.811 ± 0.033
1.31TyrAsn: 1.31 ± 0.045
1.436TyrPro: 1.436 ± 0.043
1.394TyrGln: 1.394 ± 0.044
1.664TyrArg: 1.664 ± 0.054
2.323TyrSer: 2.323 ± 0.065
2.107TyrThr: 2.107 ± 0.066
2.114TyrVal: 2.114 ± 0.059
0.301TyrTrp: 0.301 ± 0.021
1.369TyrTyr: 1.369 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2182 proteins (655076 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski