Micropruina glycogenica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Micropruina

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3901 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N9JHE7|A0A2N9JHE7_9ACTN Uncharacterized protein OS=Micropruina glycogenica OX=75385 GN=MPLG2_1938 PE=4 SV=1
MM1 pKa = 7.46TFRR4 pKa = 11.84LAATLDD10 pKa = 3.86EE11 pKa = 4.7VPVGTSIPVDD21 pKa = 3.67LGGDD25 pKa = 3.54AEE27 pKa = 4.47VAVVNTVDD35 pKa = 3.44GVFAIRR41 pKa = 11.84DD42 pKa = 3.79VCSHH46 pKa = 6.87AEE48 pKa = 3.96VPLTDD53 pKa = 4.8GEE55 pKa = 4.76VEE57 pKa = 4.17GCTIEE62 pKa = 5.48CYY64 pKa = 8.42MHH66 pKa = 7.19GSRR69 pKa = 11.84FDD71 pKa = 3.64LRR73 pKa = 11.84TGEE76 pKa = 4.24PLEE79 pKa = 4.91LPATQPVPIYY89 pKa = 9.68PVRR92 pKa = 11.84LDD94 pKa = 3.34GTDD97 pKa = 2.82IYY99 pKa = 11.75VDD101 pKa = 3.07IDD103 pKa = 3.61NPINQEE109 pKa = 3.67NN110 pKa = 3.45

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N9JL84|A0A2N9JL84_9ACTN Uncharacterized protein OS=Micropruina glycogenica OX=75385 GN=MPLG2_3298 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 8.44KK23 pKa = 9.1TRR25 pKa = 11.84IQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84AGKK33 pKa = 9.79

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3901

0

3901

1158705

20

2255

297.0

31.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.075 ± 0.056

0.796 ± 0.011

6.113 ± 0.036

5.037 ± 0.037

2.896 ± 0.023

8.835 ± 0.035

2.197 ± 0.02

3.862 ± 0.026

2.184 ± 0.03

10.441 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.017

2.129 ± 0.02

5.728 ± 0.031

3.215 ± 0.021

7.462 ± 0.049

5.438 ± 0.03

6.19 ± 0.04

8.833 ± 0.038

1.679 ± 0.02

1.987 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski