Phaeocystidibacter marisrubri

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Schleiferiaceae; Phaeocystidibacter

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L3ZF07|A0A6L3ZF07_9FLAO TolC family protein OS=Phaeocystidibacter marisrubri OX=1577780 GN=F8C82_09895 PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.47KK3 pKa = 10.35FLFFLLASLLNLSTFQSIAQGVNFEE28 pKa = 4.68LMGSFGPHH36 pKa = 4.51EE37 pKa = 4.13QVKK40 pKa = 9.88VEE42 pKa = 4.68LDD44 pKa = 3.3QLYY47 pKa = 11.03DD48 pKa = 3.6PMSDD52 pKa = 3.04IGDD55 pKa = 3.46VDD57 pKa = 3.74GDD59 pKa = 3.86GDD61 pKa = 4.73LDD63 pKa = 4.03IIIAGEE69 pKa = 4.03TGSTSRR75 pKa = 11.84ITKK78 pKa = 9.28IYY80 pKa = 9.7LYY82 pKa = 10.2NGSDD86 pKa = 3.76SYY88 pKa = 12.09DD89 pKa = 3.41SLAGIGIIGVLPRR102 pKa = 11.84ALRR105 pKa = 11.84LIDD108 pKa = 4.84LDD110 pKa = 4.09GDD112 pKa = 3.71SDD114 pKa = 5.18LDD116 pKa = 3.75VFIAGITSSNQSVTRR131 pKa = 11.84IYY133 pKa = 10.91TNDD136 pKa = 3.12GTGGFTQISSSQLPSDD152 pKa = 2.84IWSAEE157 pKa = 3.76FGDD160 pKa = 4.65LDD162 pKa = 4.14NDD164 pKa = 3.95GDD166 pKa = 3.91VDD168 pKa = 4.56LVLSTSSFNVVIFDD182 pKa = 3.78NDD184 pKa = 3.53SNASFSLRR192 pKa = 11.84SGLSLPQLKK201 pKa = 10.58ADD203 pKa = 5.13DD204 pKa = 4.73IEE206 pKa = 4.52LADD209 pKa = 3.59INYY212 pKa = 10.4DD213 pKa = 3.58SFIDD217 pKa = 3.4IVYY220 pKa = 10.27AGSVPGGAAGQLHH233 pKa = 6.57AVYY236 pKa = 10.31NDD238 pKa = 3.65SNSGFTSYY246 pKa = 11.44LLNQASFLPNGGIKK260 pKa = 9.68CANYY264 pKa = 10.67NGDD267 pKa = 3.61SLMDD271 pKa = 3.43ILYY274 pKa = 10.95VGDD277 pKa = 3.99AVSGLLLLQDD287 pKa = 3.59STGVFSLKK295 pKa = 10.85SNIYY299 pKa = 8.35PQGVLNPTIYY309 pKa = 10.53AYY311 pKa = 10.59DD312 pKa = 3.78RR313 pKa = 11.84DD314 pKa = 3.96GDD316 pKa = 3.81GDD318 pKa = 4.08EE319 pKa = 5.69DD320 pKa = 4.15ILVLGVNRR328 pKa = 11.84SGVTVYY334 pKa = 8.85HH335 pKa = 6.36TFYY338 pKa = 11.23DD339 pKa = 3.64NYY341 pKa = 10.72GDD343 pKa = 4.22GSFSPVSGHH352 pKa = 6.57GLDD355 pKa = 3.77ARR357 pKa = 11.84LPNFTPRR364 pKa = 11.84YY365 pKa = 8.86FSSQLADD372 pKa = 3.77FNGDD376 pKa = 3.39GEE378 pKa = 4.58LDD380 pKa = 3.3ILGGRR385 pKa = 11.84DD386 pKa = 3.22PGVWDD391 pKa = 5.08LYY393 pKa = 10.57HH394 pKa = 6.28GHH396 pKa = 6.55SNGKK400 pKa = 9.49FYY402 pKa = 10.7HH403 pKa = 6.78ASLSSFGNITAQNQAVADD421 pKa = 4.37FNSDD425 pKa = 3.06GALDD429 pKa = 3.74IVVVNYY435 pKa = 10.69DD436 pKa = 3.18STRR439 pKa = 11.84VYY441 pKa = 10.64FNSGTGTFPTSKK453 pKa = 10.67GLDD456 pKa = 3.14HH457 pKa = 7.33FSYY460 pKa = 10.88YY461 pKa = 9.83WGDD464 pKa = 3.18IATGDD469 pKa = 3.81FDD471 pKa = 5.38NDD473 pKa = 3.2GDD475 pKa = 3.96EE476 pKa = 5.77DD477 pKa = 4.17IVFSGSFDD485 pKa = 3.32VTKK488 pKa = 10.64IFWNNGSGDD497 pKa = 3.8FSSAPTLFTEE507 pKa = 5.28MFQQANIEE515 pKa = 4.28VFDD518 pKa = 3.9VDD520 pKa = 4.33NDD522 pKa = 3.58ADD524 pKa = 4.51LDD526 pKa = 3.92ILVTGASNSSYY537 pKa = 11.11EE538 pKa = 3.97NTALYY543 pKa = 10.1INNGSGQFVVKK554 pKa = 9.95QNSGIPPTWGSTSAQGDD571 pKa = 3.29IDD573 pKa = 4.97LDD575 pKa = 3.94GDD577 pKa = 3.61IDD579 pKa = 4.36LVLTGSPGSSIYY591 pKa = 11.06LNDD594 pKa = 3.7GQGVFTTAPMPFSTNFIMPFCEE616 pKa = 4.27LVDD619 pKa = 4.15FNGDD623 pKa = 3.56SLPDD627 pKa = 3.4MFASRR632 pKa = 11.84GSTPDD637 pKa = 2.84ATIFEE642 pKa = 4.41NLGDD646 pKa = 3.58GSFAYY651 pKa = 8.35RR652 pKa = 11.84TRR654 pKa = 11.84FGLLTQMIICDD665 pKa = 3.91ANHH668 pKa = 7.26DD669 pKa = 4.28GFPDD673 pKa = 3.26ILTGGKK679 pKa = 10.03VYY681 pKa = 11.2VNDD684 pKa = 4.15GPGNFTFTLEE694 pKa = 4.3SNTGIRR700 pKa = 11.84TEE702 pKa = 4.02NSSGGLGIAADD713 pKa = 4.2FDD715 pKa = 4.77GDD717 pKa = 3.55TDD719 pKa = 5.05DD720 pKa = 6.18DD721 pKa = 3.72IFYY724 pKa = 10.73ASAYY728 pKa = 9.12GYY730 pKa = 10.18QGNFYY735 pKa = 10.78LRR737 pKa = 11.84YY738 pKa = 6.82TQLYY742 pKa = 8.73RR743 pKa = 11.84NMAVGLSVVEE753 pKa = 4.08NTSRR757 pKa = 11.84QQGISPYY764 pKa = 8.37PNPMVSDD771 pKa = 3.59ATVDD775 pKa = 3.73LPEE778 pKa = 4.62GTFSGQIIEE787 pKa = 4.08VSGRR791 pKa = 11.84IVRR794 pKa = 11.84QINNASGNLVIEE806 pKa = 4.22RR807 pKa = 11.84EE808 pKa = 4.18GLNIGAYY815 pKa = 9.74VLVLTGEE822 pKa = 4.26NGEE825 pKa = 4.35SFTTKK830 pKa = 10.47FLVQQ834 pKa = 3.47

Molecular weight: 89.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L3ZD93|A0A6L3ZD93_9FLAO Uncharacterized protein OS=Phaeocystidibacter marisrubri OX=1577780 GN=F8C82_07895 PE=4 SV=1
MM1 pKa = 7.0NQTRR5 pKa = 11.84QKK7 pKa = 10.52RR8 pKa = 11.84LLRR11 pKa = 11.84TFEE14 pKa = 4.37FLWMIAAAVMVVEE27 pKa = 5.11CYY29 pKa = 10.27VAFSGGDD36 pKa = 3.35TTRR39 pKa = 11.84GLILLLLGLYY49 pKa = 10.53AGFRR53 pKa = 11.84YY54 pKa = 8.33VTTGMKK60 pKa = 9.68RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 9.35LL64 pKa = 3.54

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2884

0

2884

1015842

38

4451

352.2

39.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.21 ± 0.046

0.767 ± 0.018

5.772 ± 0.033

6.639 ± 0.064

4.675 ± 0.037

7.192 ± 0.051

2.115 ± 0.021

6.579 ± 0.035

5.001 ± 0.057

9.168 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.527 ± 0.023

4.947 ± 0.037

3.937 ± 0.032

3.419 ± 0.026

4.616 ± 0.045

7.227 ± 0.051

5.898 ± 0.071

6.956 ± 0.038

1.371 ± 0.019

3.984 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski