Citrobacter phage NS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9IRE7|A0A7G9IRE7_9CAUD Major capsid protein OS=Citrobacter phage NS1 OX=2766968 PE=4 SV=1
MM1 pKa = 6.87SQSVYY6 pKa = 11.38AEE8 pKa = 4.16FGVSSNAITGSVEE21 pKa = 4.85DD22 pKa = 4.5LNEE25 pKa = 4.16HH26 pKa = 5.47QKK28 pKa = 11.61SMLEE32 pKa = 3.55QDD34 pKa = 3.61VAVRR38 pKa = 11.84DD39 pKa = 3.75GDD41 pKa = 3.85DD42 pKa = 4.58AITFKK47 pKa = 10.85QLEE50 pKa = 4.4AEE52 pKa = 4.25DD53 pKa = 4.36EE54 pKa = 4.28EE55 pKa = 4.75LTGEE59 pKa = 3.84EE60 pKa = 4.49DD61 pKa = 3.67EE62 pKa = 4.35NVEE65 pKa = 4.19EE66 pKa = 4.88IEE68 pKa = 4.29GEE70 pKa = 3.98EE71 pKa = 3.95DD72 pKa = 3.63HH73 pKa = 7.31EE74 pKa = 5.84SDD76 pKa = 5.18DD77 pKa = 4.98EE78 pKa = 4.22EE79 pKa = 5.98SEE81 pKa = 4.31TDD83 pKa = 3.48GEE85 pKa = 4.22QPEE88 pKa = 4.45FVEE91 pKa = 5.88LGDD94 pKa = 3.94APKK97 pKa = 10.73EE98 pKa = 4.05LTEE101 pKa = 4.38SVAALDD107 pKa = 3.89EE108 pKa = 4.43NEE110 pKa = 4.22AAFDD114 pKa = 4.13DD115 pKa = 4.55MVSSAVEE122 pKa = 3.96AGKK125 pKa = 8.38VTADD129 pKa = 4.72DD130 pKa = 3.16ITAIKK135 pKa = 10.48AEE137 pKa = 4.04YY138 pKa = 10.7AKK140 pKa = 10.63DD141 pKa = 3.79CKK143 pKa = 10.89LSDD146 pKa = 3.15ASYY149 pKa = 11.56AKK151 pKa = 10.33LQEE154 pKa = 3.93AGYY157 pKa = 7.75TKK159 pKa = 10.56RR160 pKa = 11.84FVDD163 pKa = 3.54SFIRR167 pKa = 11.84GQEE170 pKa = 3.85ALAEE174 pKa = 4.09QYY176 pKa = 10.62AAGVVRR182 pKa = 11.84YY183 pKa = 9.78AGGAEE188 pKa = 3.89QFNRR192 pKa = 11.84ILSHH196 pKa = 7.11LEE198 pKa = 3.53STDD201 pKa = 3.08PSTRR205 pKa = 11.84EE206 pKa = 3.75ALEE209 pKa = 3.76AAIVRR214 pKa = 11.84KK215 pKa = 10.32DD216 pKa = 3.1IATTKK221 pKa = 10.96ALLNLAGKK229 pKa = 7.93TLGKK233 pKa = 10.41AVGVKK238 pKa = 8.5PQRR241 pKa = 11.84TITTQAKK248 pKa = 9.21PVVAPKK254 pKa = 10.58APQTEE259 pKa = 4.38AFSSKK264 pKa = 10.93ADD266 pKa = 3.78MIKK269 pKa = 10.77AMSDD273 pKa = 2.96PRR275 pKa = 11.84YY276 pKa = 10.34LRR278 pKa = 11.84DD279 pKa = 3.04AKK281 pKa = 10.36YY282 pKa = 9.52TMEE285 pKa = 3.95VRR287 pKa = 11.84AKK289 pKa = 10.22VAASSLL295 pKa = 3.78

Molecular weight: 31.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9IRD8|A0A7G9IRD8_9CAUD Protein suppressor of silencing OS=Citrobacter phage NS1 OX=2766968 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84FPRR5 pKa = 11.84NPKK8 pKa = 9.6RR9 pKa = 11.84QTVTSKK15 pKa = 9.46TDD17 pKa = 3.82DD18 pKa = 3.3IVRR21 pKa = 11.84HH22 pKa = 5.28FQYY25 pKa = 11.16GMTQHH30 pKa = 5.68FTGAASEE37 pKa = 4.99LYY39 pKa = 9.78TSYY42 pKa = 11.41RR43 pKa = 11.84LIAMGHH49 pKa = 4.75QVFLPAFTQSKK60 pKa = 10.4ADD62 pKa = 3.68LVVDD66 pKa = 3.89IGGTLKK72 pKa = 10.5RR73 pKa = 11.84VQVKK77 pKa = 9.16TGTKK81 pKa = 9.81LKK83 pKa = 9.73HH84 pKa = 6.38CNSIQVRR91 pKa = 11.84LGGCGKK97 pKa = 8.09PTYY100 pKa = 10.61SRR102 pKa = 11.84VII104 pKa = 3.49

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11472

38

1281

234.1

26.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.665 ± 0.419

0.994 ± 0.128

6.163 ± 0.239

6.355 ± 0.292

3.417 ± 0.194

7.932 ± 0.378

1.778 ± 0.176

4.899 ± 0.183

6.294 ± 0.306

8.29 ± 0.282

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.17

4.446 ± 0.224

3.574 ± 0.213

3.94 ± 0.366

5.666 ± 0.254

6.538 ± 0.403

6.084 ± 0.264

7.235 ± 0.277

1.621 ± 0.172

3.286 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski