Amino acid dipepetide frequency for Streptococcus phage IPP12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.59AlaAla: 4.59 ± 1.157
0.468AlaCys: 0.468 ± 0.23
5.246AlaAsp: 5.246 ± 0.756
5.995AlaGlu: 5.995 ± 0.879
2.717AlaPhe: 2.717 ± 0.458
4.309AlaGly: 4.309 ± 0.749
0.562AlaHis: 0.562 ± 0.213
5.34AlaIle: 5.34 ± 0.993
3.934AlaLys: 3.934 ± 0.573
7.681AlaLeu: 7.681 ± 0.957
2.061AlaMet: 2.061 ± 0.348
4.59AlaAsn: 4.59 ± 0.888
1.03AlaPro: 1.03 ± 0.283
3.185AlaGln: 3.185 ± 0.86
2.998AlaArg: 2.998 ± 0.428
4.215AlaSer: 4.215 ± 0.689
3.653AlaThr: 3.653 ± 0.561
3.841AlaVal: 3.841 ± 0.737
0.656AlaTrp: 0.656 ± 0.255
2.248AlaTyr: 2.248 ± 0.567
0.0AlaXaa: 0.0 ± 0.0
Cys
0.094CysAla: 0.094 ± 0.102
0.094CysCys: 0.094 ± 0.111
0.281CysAsp: 0.281 ± 0.211
0.468CysGlu: 0.468 ± 0.239
0.094CysPhe: 0.094 ± 0.104
0.281CysGly: 0.281 ± 0.159
0.281CysHis: 0.281 ± 0.157
0.281CysIle: 0.281 ± 0.187
0.562CysLys: 0.562 ± 0.206
0.656CysLeu: 0.656 ± 0.258
0.281CysMet: 0.281 ± 0.178
0.094CysAsn: 0.094 ± 0.083
0.094CysPro: 0.094 ± 0.113
0.281CysGln: 0.281 ± 0.157
0.468CysArg: 0.468 ± 0.254
0.187CysSer: 0.187 ± 0.132
0.281CysThr: 0.281 ± 0.166
0.187CysVal: 0.187 ± 0.143
0.0CysTrp: 0.0 ± 0.0
0.375CysTyr: 0.375 ± 0.226
0.0CysXaa: 0.0 ± 0.0
Asp
3.841AspAla: 3.841 ± 0.452
0.281AspCys: 0.281 ± 0.182
3.56AspAsp: 3.56 ± 0.716
4.403AspGlu: 4.403 ± 0.995
2.904AspPhe: 2.904 ± 0.558
4.496AspGly: 4.496 ± 0.849
0.749AspHis: 0.749 ± 0.257
5.808AspIle: 5.808 ± 0.661
5.059AspLys: 5.059 ± 0.642
5.527AspLeu: 5.527 ± 0.596
1.405AspMet: 1.405 ± 0.379
3.466AspAsn: 3.466 ± 0.611
1.499AspPro: 1.499 ± 0.347
1.499AspGln: 1.499 ± 0.333
1.78AspArg: 1.78 ± 0.438
3.091AspSer: 3.091 ± 0.622
2.904AspThr: 2.904 ± 0.452
3.56AspVal: 3.56 ± 0.6
0.749AspTrp: 0.749 ± 0.213
3.279AspTyr: 3.279 ± 0.563
0.0AspXaa: 0.0 ± 0.0
Glu
5.246GluAla: 5.246 ± 0.833
0.281GluCys: 0.281 ± 0.153
4.122GluAsp: 4.122 ± 0.63
6.464GluGlu: 6.464 ± 1.042
3.747GluPhe: 3.747 ± 0.643
3.185GluGly: 3.185 ± 0.562
0.843GluHis: 0.843 ± 0.282
6.276GluIle: 6.276 ± 0.839
7.4GluLys: 7.4 ± 1.192
10.211GluLeu: 10.211 ± 1.291
3.279GluMet: 3.279 ± 0.739
4.59GluAsn: 4.59 ± 0.609
1.311GluPro: 1.311 ± 0.486
3.934GluGln: 3.934 ± 0.746
3.466GluArg: 3.466 ± 0.659
4.122GluSer: 4.122 ± 0.454
4.871GluThr: 4.871 ± 0.739
4.965GluVal: 4.965 ± 0.643
1.124GluTrp: 1.124 ± 0.321
2.81GluTyr: 2.81 ± 0.53
0.0GluXaa: 0.0 ± 0.0
Phe
2.436PheAla: 2.436 ± 0.492
0.281PheCys: 0.281 ± 0.212
3.747PheAsp: 3.747 ± 0.706
2.998PheGlu: 2.998 ± 0.588
2.342PhePhe: 2.342 ± 0.703
2.81PheGly: 2.81 ± 0.623
0.562PheHis: 0.562 ± 0.244
3.747PheIle: 3.747 ± 0.646
4.496PheLys: 4.496 ± 0.697
2.81PheLeu: 2.81 ± 0.554
1.405PheMet: 1.405 ± 0.361
3.279PheAsn: 3.279 ± 0.379
1.218PhePro: 1.218 ± 0.395
1.593PheGln: 1.593 ± 0.426
1.593PheArg: 1.593 ± 0.329
2.248PheSer: 2.248 ± 0.534
2.248PheThr: 2.248 ± 0.312
2.623PheVal: 2.623 ± 0.578
0.843PheTrp: 0.843 ± 0.251
1.686PheTyr: 1.686 ± 0.514
0.0PheXaa: 0.0 ± 0.0
Gly
3.091GlyAla: 3.091 ± 0.559
0.187GlyCys: 0.187 ± 0.142
3.56GlyAsp: 3.56 ± 0.753
3.56GlyGlu: 3.56 ± 0.45
2.998GlyPhe: 2.998 ± 0.589
3.653GlyGly: 3.653 ± 0.406
0.562GlyHis: 0.562 ± 0.221
4.403GlyIle: 4.403 ± 0.526
4.122GlyLys: 4.122 ± 0.524
5.059GlyLeu: 5.059 ± 0.662
1.593GlyMet: 1.593 ± 0.525
3.185GlyAsn: 3.185 ± 0.541
0.843GlyPro: 0.843 ± 0.229
2.717GlyGln: 2.717 ± 0.584
3.279GlyArg: 3.279 ± 0.666
3.653GlySer: 3.653 ± 0.589
4.028GlyThr: 4.028 ± 0.568
3.747GlyVal: 3.747 ± 0.457
1.311GlyTrp: 1.311 ± 0.477
2.717GlyTyr: 2.717 ± 0.547
0.0GlyXaa: 0.0 ± 0.0
His
0.468HisAla: 0.468 ± 0.19
0.187HisCys: 0.187 ± 0.138
0.656HisAsp: 0.656 ± 0.308
1.311HisGlu: 1.311 ± 0.359
1.405HisPhe: 1.405 ± 0.335
0.937HisGly: 0.937 ± 0.278
0.468HisHis: 0.468 ± 0.214
1.967HisIle: 1.967 ± 0.442
0.562HisLys: 0.562 ± 0.297
1.03HisLeu: 1.03 ± 0.328
0.375HisMet: 0.375 ± 0.147
0.937HisAsn: 0.937 ± 0.301
0.562HisPro: 0.562 ± 0.2
0.656HisGln: 0.656 ± 0.326
0.843HisArg: 0.843 ± 0.338
1.124HisSer: 1.124 ± 0.435
0.749HisThr: 0.749 ± 0.322
0.749HisVal: 0.749 ± 0.266
0.187HisTrp: 0.187 ± 0.12
0.375HisTyr: 0.375 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
6.37IleAla: 6.37 ± 0.75
0.468IleCys: 0.468 ± 0.254
4.215IleAsp: 4.215 ± 0.571
5.621IleGlu: 5.621 ± 0.762
2.529IlePhe: 2.529 ± 0.425
2.904IleGly: 2.904 ± 0.664
0.937IleHis: 0.937 ± 0.272
4.122IleIle: 4.122 ± 0.635
6.276IleLys: 6.276 ± 0.923
5.246IleLeu: 5.246 ± 0.713
1.593IleMet: 1.593 ± 0.331
3.841IleAsn: 3.841 ± 0.417
1.874IlePro: 1.874 ± 0.337
2.81IleGln: 2.81 ± 0.367
3.747IleArg: 3.747 ± 0.553
5.902IleSer: 5.902 ± 0.969
4.309IleThr: 4.309 ± 0.503
4.122IleVal: 4.122 ± 0.703
0.562IleTrp: 0.562 ± 0.257
1.78IleTyr: 1.78 ± 0.489
0.0IleXaa: 0.0 ± 0.0
Lys
5.995LysAla: 5.995 ± 0.841
0.281LysCys: 0.281 ± 0.215
5.433LysAsp: 5.433 ± 0.644
7.588LysGlu: 7.588 ± 1.076
2.904LysPhe: 2.904 ± 0.562
4.403LysGly: 4.403 ± 0.751
1.78LysHis: 1.78 ± 0.349
4.871LysIle: 4.871 ± 0.658
6.745LysLys: 6.745 ± 0.923
5.808LysLeu: 5.808 ± 0.76
2.061LysMet: 2.061 ± 0.441
4.59LysAsn: 4.59 ± 0.514
3.279LysPro: 3.279 ± 0.495
4.028LysGln: 4.028 ± 0.686
4.309LysArg: 4.309 ± 0.925
4.684LysSer: 4.684 ± 0.682
5.152LysThr: 5.152 ± 0.821
4.59LysVal: 4.59 ± 0.722
1.311LysTrp: 1.311 ± 0.387
3.091LysTyr: 3.091 ± 0.584
0.0LysXaa: 0.0 ± 0.0
Leu
6.745LeuAla: 6.745 ± 0.817
0.281LeuCys: 0.281 ± 0.171
5.995LeuAsp: 5.995 ± 0.718
7.588LeuGlu: 7.588 ± 1.001
3.653LeuPhe: 3.653 ± 0.622
6.183LeuGly: 6.183 ± 0.704
1.03LeuHis: 1.03 ± 0.29
4.496LeuIle: 4.496 ± 0.607
8.15LeuLys: 8.15 ± 0.887
5.714LeuLeu: 5.714 ± 0.938
1.967LeuMet: 1.967 ± 0.553
4.59LeuAsn: 4.59 ± 0.611
2.061LeuPro: 2.061 ± 0.494
2.717LeuGln: 2.717 ± 0.796
4.215LeuArg: 4.215 ± 0.758
6.183LeuSer: 6.183 ± 0.873
5.059LeuThr: 5.059 ± 0.551
4.028LeuVal: 4.028 ± 0.674
0.562LeuTrp: 0.562 ± 0.215
2.81LeuTyr: 2.81 ± 0.584
0.0LeuXaa: 0.0 ± 0.0
Met
1.686MetAla: 1.686 ± 0.355
0.187MetCys: 0.187 ± 0.152
1.499MetAsp: 1.499 ± 0.385
2.155MetGlu: 2.155 ± 0.472
1.03MetPhe: 1.03 ± 0.337
1.218MetGly: 1.218 ± 0.263
0.094MetHis: 0.094 ± 0.1
1.593MetIle: 1.593 ± 0.327
2.155MetLys: 2.155 ± 0.527
2.342MetLeu: 2.342 ± 0.722
0.468MetMet: 0.468 ± 0.197
1.967MetAsn: 1.967 ± 0.434
0.562MetPro: 0.562 ± 0.24
0.937MetGln: 0.937 ± 0.263
1.499MetArg: 1.499 ± 0.444
1.874MetSer: 1.874 ± 0.419
1.967MetThr: 1.967 ± 0.418
1.686MetVal: 1.686 ± 0.358
0.094MetTrp: 0.094 ± 0.102
0.562MetTyr: 0.562 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
3.841AsnAla: 3.841 ± 0.764
0.094AsnCys: 0.094 ± 0.099
2.904AsnAsp: 2.904 ± 0.475
4.59AsnGlu: 4.59 ± 0.478
2.717AsnPhe: 2.717 ± 0.436
4.684AsnGly: 4.684 ± 0.78
1.218AsnHis: 1.218 ± 0.376
4.028AsnIle: 4.028 ± 0.638
5.714AsnLys: 5.714 ± 0.759
4.778AsnLeu: 4.778 ± 0.594
0.749AsnMet: 0.749 ± 0.271
2.623AsnAsn: 2.623 ± 0.497
2.81AsnPro: 2.81 ± 0.518
3.372AsnGln: 3.372 ± 0.591
2.061AsnArg: 2.061 ± 0.524
3.56AsnSer: 3.56 ± 0.651
2.81AsnThr: 2.81 ± 0.416
3.279AsnVal: 3.279 ± 0.558
0.562AsnTrp: 0.562 ± 0.226
0.843AsnTyr: 0.843 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
2.248ProAla: 2.248 ± 0.475
0.281ProCys: 0.281 ± 0.141
1.874ProAsp: 1.874 ± 0.56
1.967ProGlu: 1.967 ± 0.33
0.937ProPhe: 0.937 ± 0.311
0.656ProGly: 0.656 ± 0.295
0.749ProHis: 0.749 ± 0.254
1.78ProIle: 1.78 ± 0.476
2.248ProLys: 2.248 ± 0.561
2.436ProLeu: 2.436 ± 0.512
0.187ProMet: 0.187 ± 0.109
0.656ProAsn: 0.656 ± 0.293
0.375ProPro: 0.375 ± 0.21
1.311ProGln: 1.311 ± 0.417
0.843ProArg: 0.843 ± 0.258
1.593ProSer: 1.593 ± 0.382
1.874ProThr: 1.874 ± 0.576
2.342ProVal: 2.342 ± 0.459
0.468ProTrp: 0.468 ± 0.179
1.03ProTyr: 1.03 ± 0.366
0.0ProXaa: 0.0 ± 0.0
Gln
4.871GlnAla: 4.871 ± 0.982
0.094GlnCys: 0.094 ± 0.113
1.218GlnAsp: 1.218 ± 0.343
4.59GlnGlu: 4.59 ± 0.564
1.593GlnPhe: 1.593 ± 0.437
1.967GlnGly: 1.967 ± 0.465
0.656GlnHis: 0.656 ± 0.226
2.342GlnIle: 2.342 ± 0.486
3.372GlnLys: 3.372 ± 0.472
2.904GlnLeu: 2.904 ± 0.479
1.311GlnMet: 1.311 ± 0.391
2.81GlnAsn: 2.81 ± 0.567
1.499GlnPro: 1.499 ± 0.301
1.405GlnGln: 1.405 ± 0.406
2.155GlnArg: 2.155 ± 0.474
2.529GlnSer: 2.529 ± 0.415
2.623GlnThr: 2.623 ± 0.678
2.623GlnVal: 2.623 ± 0.474
0.281GlnTrp: 0.281 ± 0.18
0.843GlnTyr: 0.843 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
3.279ArgAla: 3.279 ± 0.579
0.281ArgCys: 0.281 ± 0.136
2.342ArgAsp: 2.342 ± 0.421
3.934ArgGlu: 3.934 ± 0.677
1.967ArgPhe: 1.967 ± 0.635
1.593ArgGly: 1.593 ± 0.292
0.749ArgHis: 0.749 ± 0.261
3.279ArgIle: 3.279 ± 0.702
3.934ArgLys: 3.934 ± 0.8
4.028ArgLeu: 4.028 ± 0.62
1.311ArgMet: 1.311 ± 0.361
3.56ArgAsn: 3.56 ± 0.422
1.218ArgPro: 1.218 ± 0.349
2.248ArgGln: 2.248 ± 0.515
2.529ArgArg: 2.529 ± 0.633
1.874ArgSer: 1.874 ± 0.48
2.623ArgThr: 2.623 ± 0.716
2.623ArgVal: 2.623 ± 0.533
0.937ArgTrp: 0.937 ± 0.304
2.81ArgTyr: 2.81 ± 0.717
0.0ArgXaa: 0.0 ± 0.0
Ser
4.965SerAla: 4.965 ± 0.801
0.281SerCys: 0.281 ± 0.144
3.747SerAsp: 3.747 ± 0.701
5.059SerGlu: 5.059 ± 0.887
3.091SerPhe: 3.091 ± 0.54
3.747SerGly: 3.747 ± 0.597
1.124SerHis: 1.124 ± 0.444
3.372SerIle: 3.372 ± 0.744
4.309SerLys: 4.309 ± 0.568
4.122SerLeu: 4.122 ± 0.515
1.593SerMet: 1.593 ± 0.541
3.653SerAsn: 3.653 ± 0.677
1.593SerPro: 1.593 ± 0.3
2.436SerGln: 2.436 ± 0.481
3.091SerArg: 3.091 ± 0.7
3.466SerSer: 3.466 ± 0.74
3.372SerThr: 3.372 ± 0.737
3.934SerVal: 3.934 ± 0.805
1.03SerTrp: 1.03 ± 0.281
2.155SerTyr: 2.155 ± 0.373
0.0SerXaa: 0.0 ± 0.0
Thr
4.309ThrAla: 4.309 ± 0.822
0.187ThrCys: 0.187 ± 0.144
4.871ThrAsp: 4.871 ± 0.859
4.309ThrGlu: 4.309 ± 0.663
3.279ThrPhe: 3.279 ± 0.618
3.747ThrGly: 3.747 ± 0.874
1.124ThrHis: 1.124 ± 0.291
4.778ThrIle: 4.778 ± 0.719
4.496ThrLys: 4.496 ± 0.512
4.496ThrLeu: 4.496 ± 0.694
1.124ThrMet: 1.124 ± 0.342
2.81ThrAsn: 2.81 ± 0.449
0.656ThrPro: 0.656 ± 0.191
2.623ThrGln: 2.623 ± 0.717
2.717ThrArg: 2.717 ± 0.464
3.279ThrSer: 3.279 ± 0.529
4.309ThrThr: 4.309 ± 0.917
4.403ThrVal: 4.403 ± 0.834
0.843ThrTrp: 0.843 ± 0.258
2.155ThrTyr: 2.155 ± 0.511
0.0ThrXaa: 0.0 ± 0.0
Val
2.623ValAla: 2.623 ± 0.384
0.375ValCys: 0.375 ± 0.21
2.717ValAsp: 2.717 ± 0.468
5.527ValGlu: 5.527 ± 0.809
2.529ValPhe: 2.529 ± 0.462
4.496ValGly: 4.496 ± 0.761
1.124ValHis: 1.124 ± 0.363
3.934ValIle: 3.934 ± 0.538
6.089ValLys: 6.089 ± 0.779
4.215ValLeu: 4.215 ± 0.636
1.218ValMet: 1.218 ± 0.342
3.091ValAsn: 3.091 ± 0.487
1.593ValPro: 1.593 ± 0.362
1.78ValGln: 1.78 ± 0.367
2.436ValArg: 2.436 ± 0.461
3.934ValSer: 3.934 ± 0.781
5.621ValThr: 5.621 ± 0.683
4.778ValVal: 4.778 ± 0.621
1.03ValTrp: 1.03 ± 0.371
1.499ValTyr: 1.499 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
0.656TrpAla: 0.656 ± 0.249
0.094TrpCys: 0.094 ± 0.1
0.468TrpAsp: 0.468 ± 0.198
1.311TrpGlu: 1.311 ± 0.365
0.468TrpPhe: 0.468 ± 0.21
0.843TrpGly: 0.843 ± 0.251
0.281TrpHis: 0.281 ± 0.164
0.749TrpIle: 0.749 ± 0.299
0.843TrpLys: 0.843 ± 0.311
1.03TrpLeu: 1.03 ± 0.28
0.468TrpMet: 0.468 ± 0.174
1.499TrpAsn: 1.499 ± 0.342
0.187TrpPro: 0.187 ± 0.12
0.656TrpGln: 0.656 ± 0.231
0.375TrpArg: 0.375 ± 0.204
1.03TrpSer: 1.03 ± 0.328
0.749TrpThr: 0.749 ± 0.244
0.562TrpVal: 0.562 ± 0.226
0.187TrpTrp: 0.187 ± 0.107
0.937TrpTyr: 0.937 ± 0.566
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.061TyrAla: 2.061 ± 0.366
0.656TyrCys: 0.656 ± 0.256
1.405TyrAsp: 1.405 ± 0.363
2.81TyrGlu: 2.81 ± 0.547
2.061TyrPhe: 2.061 ± 0.397
2.155TyrGly: 2.155 ± 0.424
0.656TyrHis: 0.656 ± 0.205
2.061TyrIle: 2.061 ± 0.309
2.436TyrLys: 2.436 ± 0.431
3.934TyrLeu: 3.934 ± 0.573
1.03TyrMet: 1.03 ± 0.356
1.405TyrAsn: 1.405 ± 0.372
1.593TyrPro: 1.593 ± 0.465
1.499TyrGln: 1.499 ± 0.401
2.717TyrArg: 2.717 ± 0.583
1.593TyrSer: 1.593 ± 0.315
1.311TyrThr: 1.311 ± 0.322
1.967TyrVal: 1.967 ± 0.334
0.656TyrTrp: 0.656 ± 0.272
2.155TyrTyr: 2.155 ± 0.681
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (10676 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski