Amino acid dipepetide frequency for Mycoplasma equirhinis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.903AlaAla: 3.903 ± 0.208
0.432AlaCys: 0.432 ± 0.042
2.381AlaAsp: 2.381 ± 0.115
3.447AlaGlu: 3.447 ± 0.221
2.762AlaPhe: 2.762 ± 0.118
2.837AlaGly: 2.837 ± 0.124
0.855AlaHis: 0.855 ± 0.069
6.726AlaIle: 6.726 ± 0.218
7.21AlaLys: 7.21 ± 0.313
5.434AlaLeu: 5.434 ± 0.168
1.24AlaMet: 1.24 ± 0.089
4.636AlaAsn: 4.636 ± 0.17
1.367AlaPro: 1.367 ± 0.096
1.869AlaGln: 1.869 ± 0.098
2.231AlaArg: 2.231 ± 0.109
3.485AlaSer: 3.485 ± 0.139
3.368AlaThr: 3.368 ± 0.117
2.405AlaVal: 2.405 ± 0.126
0.395AlaTrp: 0.395 ± 0.048
2.104AlaTyr: 2.104 ± 0.092
0.0AlaXaa: 0.0 ± 0.0
Cys
0.451CysAla: 0.451 ± 0.044
0.07CysCys: 0.07 ± 0.019
0.427CysAsp: 0.427 ± 0.049
0.488CysGlu: 0.488 ± 0.047
0.329CysPhe: 0.329 ± 0.036
0.474CysGly: 0.474 ± 0.051
0.122CysHis: 0.122 ± 0.023
0.446CysIle: 0.446 ± 0.044
0.526CysLys: 0.526 ± 0.052
0.686CysLeu: 0.686 ± 0.053
0.075CysMet: 0.075 ± 0.02
0.423CysAsn: 0.423 ± 0.041
0.23CysPro: 0.23 ± 0.033
0.211CysGln: 0.211 ± 0.035
0.202CysArg: 0.202 ± 0.033
0.38CysSer: 0.38 ± 0.044
0.254CysThr: 0.254 ± 0.035
0.399CysVal: 0.399 ± 0.047
0.047CysTrp: 0.047 ± 0.016
0.211CysTyr: 0.211 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
3.499AspAla: 3.499 ± 0.192
0.24AspCys: 0.24 ± 0.032
2.602AspAsp: 2.602 ± 0.126
4.594AspGlu: 4.594 ± 0.168
3.64AspPhe: 3.64 ± 0.157
2.56AspGly: 2.56 ± 0.131
0.7AspHis: 0.7 ± 0.059
4.852AspIle: 4.852 ± 0.165
5.848AspLys: 5.848 ± 0.25
5.246AspLeu: 5.246 ± 0.162
0.728AspMet: 0.728 ± 0.06
3.349AspAsn: 3.349 ± 0.129
1.578AspPro: 1.578 ± 0.104
1.386AspGln: 1.386 ± 0.073
1.386AspArg: 1.386 ± 0.094
3.316AspSer: 3.316 ± 0.132
2.24AspThr: 2.24 ± 0.121
2.696AspVal: 2.696 ± 0.135
0.479AspTrp: 0.479 ± 0.046
2.456AspTyr: 2.456 ± 0.115
0.0AspXaa: 0.0 ± 0.0
Glu
4.133GluAla: 4.133 ± 0.296
0.348GluCys: 0.348 ± 0.041
3.184GluAsp: 3.184 ± 0.131
4.833GluGlu: 4.833 ± 0.204
3.579GluPhe: 3.579 ± 0.141
2.48GluGly: 2.48 ± 0.121
0.93GluHis: 0.93 ± 0.071
8.262GluIle: 8.262 ± 0.194
8.482GluLys: 8.482 ± 0.256
7.257GluLeu: 7.257 ± 0.206
1.404GluMet: 1.404 ± 0.084
6.759GluAsn: 6.759 ± 0.188
1.39GluPro: 1.39 ± 0.092
2.142GluGln: 2.142 ± 0.085
1.959GluArg: 1.959 ± 0.115
3.49GluSer: 3.49 ± 0.15
3.494GluThr: 3.494 ± 0.139
3.368GluVal: 3.368 ± 0.12
0.62GluTrp: 0.62 ± 0.049
3.015GluTyr: 3.015 ± 0.106
0.0GluXaa: 0.0 ± 0.0
Phe
3.274PheAla: 3.274 ± 0.135
0.474PheCys: 0.474 ± 0.049
3.574PheAsp: 3.574 ± 0.131
3.837PheGlu: 3.837 ± 0.146
2.564PhePhe: 2.564 ± 0.14
2.766PheGly: 2.766 ± 0.133
0.681PheHis: 0.681 ± 0.058
4.969PheIle: 4.969 ± 0.214
4.899PheLys: 4.899 ± 0.17
4.866PheLeu: 4.866 ± 0.183
0.836PheMet: 0.836 ± 0.065
3.903PheAsn: 3.903 ± 0.17
1.198PhePro: 1.198 ± 0.086
1.188PheGln: 1.188 ± 0.082
1.325PheArg: 1.325 ± 0.08
4.269PheSer: 4.269 ± 0.165
2.715PheThr: 2.715 ± 0.126
3.072PheVal: 3.072 ± 0.122
0.573PheTrp: 0.573 ± 0.051
1.949PheTyr: 1.949 ± 0.1
0.0PheXaa: 0.0 ± 0.0
Gly
2.837GlyAla: 2.837 ± 0.13
0.277GlyCys: 0.277 ± 0.04
2.452GlyAsp: 2.452 ± 0.14
2.682GlyGlu: 2.682 ± 0.12
2.691GlyPhe: 2.691 ± 0.14
2.954GlyGly: 2.954 ± 0.177
0.935GlyHis: 0.935 ± 0.078
4.857GlyIle: 4.857 ± 0.182
4.622GlyLys: 4.622 ± 0.145
3.955GlyLeu: 3.955 ± 0.176
1.104GlyMet: 1.104 ± 0.081
2.607GlyAsn: 2.607 ± 0.114
1.113GlyPro: 1.113 ± 0.095
1.545GlyGln: 1.545 ± 0.094
1.7GlyArg: 1.7 ± 0.119
2.95GlySer: 2.95 ± 0.129
2.301GlyThr: 2.301 ± 0.14
2.616GlyVal: 2.616 ± 0.116
0.484GlyTrp: 0.484 ± 0.049
2.095GlyTyr: 2.095 ± 0.107
0.0GlyXaa: 0.0 ± 0.0
His
0.93HisAla: 0.93 ± 0.066
0.108HisCys: 0.108 ± 0.026
0.813HisAsp: 0.813 ± 0.072
0.991HisGlu: 0.991 ± 0.062
0.836HisPhe: 0.836 ± 0.069
0.906HisGly: 0.906 ± 0.069
0.287HisHis: 0.287 ± 0.038
1.254HisIle: 1.254 ± 0.088
1.179HisLys: 1.179 ± 0.067
1.395HisLeu: 1.395 ± 0.105
0.254HisMet: 0.254 ± 0.038
0.996HisAsn: 0.996 ± 0.074
0.535HisPro: 0.535 ± 0.051
0.39HisGln: 0.39 ± 0.047
0.503HisArg: 0.503 ± 0.045
1.057HisSer: 1.057 ± 0.074
0.653HisThr: 0.653 ± 0.056
0.7HisVal: 0.7 ± 0.074
0.127HisTrp: 0.127 ± 0.024
0.568HisTyr: 0.568 ± 0.057
0.0HisXaa: 0.0 ± 0.0
Ile
6.28IleAla: 6.28 ± 0.193
0.822IleCys: 0.822 ± 0.069
6.416IleAsp: 6.416 ± 0.167
7.181IleGlu: 7.181 ± 0.216
5.547IlePhe: 5.547 ± 0.25
4.34IleGly: 4.34 ± 0.159
1.198IleHis: 1.198 ± 0.088
9.333IleIle: 9.333 ± 0.308
9.779IleLys: 9.779 ± 0.232
8.905IleLeu: 8.905 ± 0.277
1.597IleMet: 1.597 ± 0.092
8.412IleAsn: 8.412 ± 0.26
2.691IlePro: 2.691 ± 0.122
2.55IleGln: 2.55 ± 0.13
2.842IleArg: 2.842 ± 0.114
7.459IleSer: 7.459 ± 0.219
5.002IleThr: 5.002 ± 0.156
5.5IleVal: 5.5 ± 0.191
0.733IleTrp: 0.733 ± 0.061
3.851IleTyr: 3.851 ± 0.146
0.0IleXaa: 0.0 ± 0.0
Lys
5.209LysAla: 5.209 ± 0.288
0.531LysCys: 0.531 ± 0.053
5.303LysAsp: 5.303 ± 0.202
7.853LysGlu: 7.853 ± 0.238
4.758LysPhe: 4.758 ± 0.198
3.339LysGly: 3.339 ± 0.143
1.437LysHis: 1.437 ± 0.076
11.55LysIle: 11.55 ± 0.299
11.108LysLys: 11.108 ± 0.297
10.183LysLeu: 10.183 ± 0.275
2.442LysMet: 2.442 ± 0.117
9.638LysAsn: 9.638 ± 0.301
2.471LysPro: 2.471 ± 0.112
3.87LysGln: 3.87 ± 0.207
3.119LysArg: 3.119 ± 0.134
6.158LysSer: 6.158 ± 0.166
5.721LysThr: 5.721 ± 0.156
4.528LysVal: 4.528 ± 0.169
0.996LysTrp: 0.996 ± 0.078
5.077LysTyr: 5.077 ± 0.171
0.0LysXaa: 0.0 ± 0.0
Leu
6.214LeuAla: 6.214 ± 0.163
0.531LeuCys: 0.531 ± 0.052
5.411LeuAsp: 5.411 ± 0.182
6.698LeuGlu: 6.698 ± 0.212
5.035LeuPhe: 5.035 ± 0.211
4.631LeuGly: 4.631 ± 0.158
1.353LeuHis: 1.353 ± 0.084
9.22LeuIle: 9.22 ± 0.301
9.784LeuLys: 9.784 ± 0.257
8.558LeuLeu: 8.558 ± 0.271
1.555LeuMet: 1.555 ± 0.092
7.623LeuAsn: 7.623 ± 0.246
2.804LeuPro: 2.804 ± 0.125
2.471LeuGln: 2.471 ± 0.122
3.044LeuArg: 3.044 ± 0.141
6.561LeuSer: 6.561 ± 0.181
4.95LeuThr: 4.95 ± 0.165
4.692LeuVal: 4.692 ± 0.151
0.719LeuTrp: 0.719 ± 0.057
2.832LeuTyr: 2.832 ± 0.109
0.0LeuXaa: 0.0 ± 0.0
Met
1.226MetAla: 1.226 ± 0.078
0.113MetCys: 0.113 ± 0.024
0.733MetAsp: 0.733 ± 0.071
0.822MetGlu: 0.822 ± 0.066
0.977MetPhe: 0.977 ± 0.068
0.813MetGly: 0.813 ± 0.064
0.333MetHis: 0.333 ± 0.041
1.63MetIle: 1.63 ± 0.086
1.789MetLys: 1.789 ± 0.085
1.724MetLeu: 1.724 ± 0.102
0.357MetMet: 0.357 ± 0.047
1.207MetAsn: 1.207 ± 0.08
0.798MetPro: 0.798 ± 0.071
0.789MetGln: 0.789 ± 0.066
0.545MetArg: 0.545 ± 0.05
1.202MetSer: 1.202 ± 0.068
0.921MetThr: 0.921 ± 0.069
0.813MetVal: 0.813 ± 0.063
0.15MetTrp: 0.15 ± 0.028
0.559MetTyr: 0.559 ± 0.057
0.0MetXaa: 0.0 ± 0.0
Asn
3.875AsnAla: 3.875 ± 0.15
0.474AsnCys: 0.474 ± 0.047
4.237AsnAsp: 4.237 ± 0.157
6.627AsnGlu: 6.627 ± 0.185
4.335AsnPhe: 4.335 ± 0.174
3.241AsnGly: 3.241 ± 0.14
0.958AsnHis: 0.958 ± 0.072
7.693AsnIle: 7.693 ± 0.252
8.778AsnLys: 8.778 ± 0.293
7.468AsnLeu: 7.468 ± 0.193
1.127AsnMet: 1.127 ± 0.071
6.167AsnAsn: 6.167 ± 0.232
2.344AsnPro: 2.344 ± 0.121
2.555AsnGln: 2.555 ± 0.106
1.968AsnArg: 1.968 ± 0.1
5.378AsnSer: 5.378 ± 0.199
2.856AsnThr: 2.856 ± 0.118
3.635AsnVal: 3.635 ± 0.125
0.803AsnTrp: 0.803 ± 0.062
3.33AsnTyr: 3.33 ± 0.143
0.0AsnXaa: 0.0 ± 0.0
Pro
1.433ProAla: 1.433 ± 0.103
0.207ProCys: 0.207 ± 0.03
1.334ProAsp: 1.334 ± 0.1
2.081ProGlu: 2.081 ± 0.12
1.343ProPhe: 1.343 ± 0.088
1.437ProGly: 1.437 ± 0.093
0.46ProHis: 0.46 ± 0.049
2.715ProIle: 2.715 ± 0.123
2.658ProLys: 2.658 ± 0.119
2.269ProLeu: 2.269 ± 0.115
0.465ProMet: 0.465 ± 0.048
2.024ProAsn: 2.024 ± 0.093
0.606ProPro: 0.606 ± 0.079
0.906ProGln: 0.906 ± 0.072
0.756ProArg: 0.756 ± 0.07
1.55ProSer: 1.55 ± 0.093
1.597ProThr: 1.597 ± 0.083
1.306ProVal: 1.306 ± 0.097
0.343ProTrp: 0.343 ± 0.048
1.193ProTyr: 1.193 ± 0.082
0.0ProXaa: 0.0 ± 0.0
Gln
1.724GlnAla: 1.724 ± 0.09
0.08GlnCys: 0.08 ± 0.017
1.559GlnAsp: 1.559 ± 0.125
2.034GlnGlu: 2.034 ± 0.119
1.339GlnPhe: 1.339 ± 0.076
1.141GlnGly: 1.141 ± 0.078
0.465GlnHis: 0.465 ± 0.055
3.509GlnIle: 3.509 ± 0.168
3.927GlnLys: 3.927 ± 0.163
2.804GlnLeu: 2.804 ± 0.121
0.54GlnMet: 0.54 ± 0.05
2.912GlnAsn: 2.912 ± 0.127
0.658GlnPro: 0.658 ± 0.058
0.831GlnGln: 0.831 ± 0.067
0.986GlnArg: 0.986 ± 0.069
1.512GlnSer: 1.512 ± 0.079
1.611GlnThr: 1.611 ± 0.098
1.278GlnVal: 1.278 ± 0.08
0.235GlnTrp: 0.235 ± 0.033
1.447GlnTyr: 1.447 ± 0.083
0.0GlnXaa: 0.0 ± 0.0
Arg
1.954ArgAla: 1.954 ± 0.12
0.254ArgCys: 0.254 ± 0.038
1.728ArgAsp: 1.728 ± 0.103
2.32ArgGlu: 2.32 ± 0.11
1.512ArgPhe: 1.512 ± 0.084
1.55ArgGly: 1.55 ± 0.12
0.545ArgHis: 0.545 ± 0.057
2.997ArgIle: 2.997 ± 0.136
3.044ArgLys: 3.044 ± 0.135
2.499ArgLeu: 2.499 ± 0.116
0.643ArgMet: 0.643 ± 0.064
1.987ArgAsn: 1.987 ± 0.097
0.902ArgPro: 0.902 ± 0.085
1.231ArgGln: 1.231 ± 0.07
1.263ArgArg: 1.263 ± 0.089
1.498ArgSer: 1.498 ± 0.081
1.418ArgThr: 1.418 ± 0.092
1.536ArgVal: 1.536 ± 0.094
0.282ArgTrp: 0.282 ± 0.042
1.254ArgTyr: 1.254 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
3.452SerAla: 3.452 ± 0.115
0.47SerCys: 0.47 ± 0.038
3.386SerAsp: 3.386 ± 0.157
4.626SerGlu: 4.626 ± 0.163
3.57SerPhe: 3.57 ± 0.159
3.433SerGly: 3.433 ± 0.142
0.93SerHis: 0.93 ± 0.072
6.139SerIle: 6.139 ± 0.208
7.214SerLys: 7.214 ± 0.202
6.646SerLeu: 6.646 ± 0.168
0.831SerMet: 0.831 ± 0.063
4.612SerAsn: 4.612 ± 0.157
1.672SerPro: 1.672 ± 0.092
2.386SerGln: 2.386 ± 0.121
1.789SerArg: 1.789 ± 0.095
4.279SerSer: 4.279 ± 0.16
2.898SerThr: 2.898 ± 0.116
3.076SerVal: 3.076 ± 0.126
0.535SerTrp: 0.535 ± 0.055
2.546SerTyr: 2.546 ± 0.121
0.0SerXaa: 0.0 ± 0.0
Thr
2.532ThrAla: 2.532 ± 0.128
0.244ThrCys: 0.244 ± 0.033
2.17ThrAsp: 2.17 ± 0.117
2.959ThrGlu: 2.959 ± 0.13
2.569ThrPhe: 2.569 ± 0.117
2.541ThrGly: 2.541 ± 0.152
0.827ThrHis: 0.827 ± 0.063
5.57ThrIle: 5.57 ± 0.212
5.181ThrLys: 5.181 ± 0.169
4.946ThrLeu: 4.946 ± 0.167
0.719ThrMet: 0.719 ± 0.055
3.814ThrAsn: 3.814 ± 0.16
1.71ThrPro: 1.71 ± 0.083
1.461ThrGln: 1.461 ± 0.095
1.644ThrArg: 1.644 ± 0.094
3.241ThrSer: 3.241 ± 0.151
2.64ThrThr: 2.64 ± 0.12
2.245ThrVal: 2.245 ± 0.112
0.442ThrTrp: 0.442 ± 0.048
1.926ThrTyr: 1.926 ± 0.097
0.0ThrXaa: 0.0 ± 0.0
Val
3.476ValAla: 3.476 ± 0.155
0.465ValCys: 0.465 ± 0.05
2.889ValAsp: 2.889 ± 0.123
3.335ValGlu: 3.335 ± 0.142
2.63ValPhe: 2.63 ± 0.125
2.776ValGly: 2.776 ± 0.139
0.719ValHis: 0.719 ± 0.064
4.396ValIle: 4.396 ± 0.152
4.537ValLys: 4.537 ± 0.16
4.673ValLeu: 4.673 ± 0.193
0.817ValMet: 0.817 ± 0.064
3.335ValAsn: 3.335 ± 0.128
1.381ValPro: 1.381 ± 0.097
1.146ValGln: 1.146 ± 0.074
1.451ValArg: 1.451 ± 0.097
3.574ValSer: 3.574 ± 0.156
2.654ValThr: 2.654 ± 0.124
2.682ValVal: 2.682 ± 0.136
0.54ValTrp: 0.54 ± 0.054
1.728ValTyr: 1.728 ± 0.099
0.0ValXaa: 0.0 ± 0.0
Trp
0.493TrpAla: 0.493 ± 0.049
0.038TrpCys: 0.038 ± 0.012
0.503TrpAsp: 0.503 ± 0.047
0.643TrpGlu: 0.643 ± 0.053
0.535TrpPhe: 0.535 ± 0.049
0.46TrpGly: 0.46 ± 0.044
0.117TrpHis: 0.117 ± 0.028
0.85TrpIle: 0.85 ± 0.069
0.883TrpLys: 0.883 ± 0.065
0.85TrpLeu: 0.85 ± 0.062
0.23TrpMet: 0.23 ± 0.031
0.737TrpAsn: 0.737 ± 0.064
0.216TrpPro: 0.216 ± 0.033
0.277TrpGln: 0.277 ± 0.042
0.366TrpArg: 0.366 ± 0.047
0.409TrpSer: 0.409 ± 0.046
0.46TrpThr: 0.46 ± 0.047
0.488TrpVal: 0.488 ± 0.055
0.108TrpTrp: 0.108 ± 0.022
0.366TrpTyr: 0.366 ± 0.044
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.193TyrAla: 2.193 ± 0.106
0.277TyrCys: 0.277 ± 0.039
2.292TyrAsp: 2.292 ± 0.102
3.076TyrGlu: 3.076 ± 0.114
2.381TyrPhe: 2.381 ± 0.116
2.095TyrGly: 2.095 ± 0.114
0.629TyrHis: 0.629 ± 0.054
3.476TyrIle: 3.476 ± 0.146
3.842TyrLys: 3.842 ± 0.144
4.321TyrLeu: 4.321 ± 0.153
0.535TyrMet: 0.535 ± 0.052
2.71TyrAsn: 2.71 ± 0.122
1.019TyrPro: 1.019 ± 0.074
1.386TyrGln: 1.386 ± 0.072
1.334TyrArg: 1.334 ± 0.087
2.724TyrSer: 2.724 ± 0.108
1.625TyrThr: 1.625 ± 0.097
2.17TyrVal: 2.17 ± 0.105
0.423TyrTrp: 0.423 ± 0.046
1.681TyrTyr: 1.681 ± 0.098
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 613 proteins (212910 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski