Miniimonas sp. PCH200

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Beutenbergiaceae; Serinibacter

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3302 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U1ZWH3|A0A2U1ZWH3_9MICO OLD family endonuclease OS=Miniimonas sp. PCH200 OX=2135789 GN=C8046_12275 PE=4 SV=1
MM1 pKa = 7.25RR2 pKa = 11.84TSPTATATLALACTLALAACSPAVDD27 pKa = 5.0DD28 pKa = 5.67GGATPALPPTSGPFDD43 pKa = 3.76YY44 pKa = 11.0QLGEE48 pKa = 4.14PYY50 pKa = 10.92GEE52 pKa = 4.11AGEE55 pKa = 4.19FAVVGRR61 pKa = 11.84DD62 pKa = 3.4VTADD66 pKa = 3.81PLEE69 pKa = 4.39GAYY72 pKa = 9.71NVCYY76 pKa = 10.77LNGFQTQPGALTSWEE91 pKa = 4.54DD92 pKa = 3.55EE93 pKa = 4.47HH94 pKa = 8.49PDD96 pKa = 3.15ALLRR100 pKa = 11.84EE101 pKa = 4.59DD102 pKa = 4.33GALVMDD108 pKa = 5.65PDD110 pKa = 3.91WPDD113 pKa = 3.25EE114 pKa = 4.05AVLDD118 pKa = 4.28PTTPDD123 pKa = 2.88QRR125 pKa = 11.84EE126 pKa = 4.06AILAVVGPQIDD137 pKa = 3.57TCAEE141 pKa = 3.89KK142 pKa = 11.03GFDD145 pKa = 4.87AVEE148 pKa = 4.28LDD150 pKa = 3.85NLDD153 pKa = 3.3TFLRR157 pKa = 11.84FDD159 pKa = 3.4GVARR163 pKa = 11.84EE164 pKa = 4.15GALALASEE172 pKa = 4.94YY173 pKa = 10.89VGRR176 pKa = 11.84AHH178 pKa = 7.92DD179 pKa = 4.37AGLAVGQKK187 pKa = 9.39NAADD191 pKa = 3.44VGEE194 pKa = 4.43AARR197 pKa = 11.84EE198 pKa = 3.71EE199 pKa = 4.29AGFDD203 pKa = 3.37FAVVEE208 pKa = 4.48EE209 pKa = 4.35CAVYY213 pKa = 10.28DD214 pKa = 3.24EE215 pKa = 5.03CGAYY219 pKa = 9.77RR220 pKa = 11.84EE221 pKa = 4.52VYY223 pKa = 10.12GEE225 pKa = 4.1HH226 pKa = 6.23VLQIEE231 pKa = 4.4YY232 pKa = 10.9SDD234 pKa = 4.01TLDD237 pKa = 4.63DD238 pKa = 5.62ALTFDD243 pKa = 5.36DD244 pKa = 4.65VCADD248 pKa = 3.93PDD250 pKa = 3.9RR251 pKa = 11.84APLTVLRR258 pKa = 11.84DD259 pKa = 3.65RR260 pKa = 11.84LLVGSDD266 pKa = 3.59SPDD269 pKa = 3.24HH270 pKa = 6.71EE271 pKa = 5.29RR272 pKa = 11.84EE273 pKa = 3.9QCPP276 pKa = 3.29

Molecular weight: 29.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U1ZUG1|A0A2U1ZUG1_9MICO DUF3048 domain-containing protein OS=Miniimonas sp. PCH200 OX=2135789 GN=C8046_07755 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3302

0

3302

1140190

24

2464

345.3

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.146 ± 0.067

0.509 ± 0.009

6.287 ± 0.038

5.56 ± 0.042

2.558 ± 0.026

9.481 ± 0.044

2.007 ± 0.023

3.321 ± 0.03

1.263 ± 0.025

10.193 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.516 ± 0.015

1.616 ± 0.028

6.033 ± 0.034

2.503 ± 0.023

7.49 ± 0.056

5.469 ± 0.03

6.819 ± 0.057

9.896 ± 0.049

1.548 ± 0.019

1.785 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski