Pectobacterium phage Jarilo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Jarilovirus; Pectobacterium virus Jarilo

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GSX8|A0A2S1GSX8_9CAUD Endonuclease OS=Pectobacterium phage Jarilo OX=2163634 PE=4 SV=1
MM1 pKa = 7.23TPLEE5 pKa = 4.25IMLSLVLTLIIGYY18 pKa = 9.81KK19 pKa = 7.74IGKK22 pKa = 8.63YY23 pKa = 8.64VAEE26 pKa = 5.53DD27 pKa = 4.35DD28 pKa = 5.72DD29 pKa = 7.0DD30 pKa = 5.63DD31 pKa = 5.55FPFLL35 pKa = 4.53

Molecular weight: 3.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GSX7|A0A2S1GSX7_9CAUD Uncharacterized protein OS=Pectobacterium phage Jarilo OX=2163634 PE=4 SV=1
MM1 pKa = 7.46IALLMVASYY10 pKa = 11.31SLIIGLFVRR19 pKa = 11.84DD20 pKa = 5.01FIKK23 pKa = 10.67GLKK26 pKa = 7.44VHH28 pKa = 6.98KK29 pKa = 9.65ATLSYY34 pKa = 10.69RR35 pKa = 11.84LYY37 pKa = 10.68GVVPRR42 pKa = 11.84FTVRR46 pKa = 11.84LTNGRR51 pKa = 11.84FKK53 pKa = 11.1ANKK56 pKa = 9.12VGVFYY61 pKa = 11.11VATHH65 pKa = 6.31

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12192

33

1327

265.0

29.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.662 ± 0.427

0.853 ± 0.16

6.389 ± 0.221

6.078 ± 0.303

3.642 ± 0.187

7.292 ± 0.325

1.878 ± 0.176

5.077 ± 0.167

6.217 ± 0.316

8.407 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.781 ± 0.154

4.429 ± 0.182

3.74 ± 0.185

4.454 ± 0.287

5.192 ± 0.185

6.406 ± 0.328

6.094 ± 0.305

6.709 ± 0.261

1.46 ± 0.185

3.24 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski