Corynebacterium phage EmiRose

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VNX4|A0A649VNX4_9CAUD Portal protein OS=Corynebacterium phage EmiRose OX=2565372 GN=3 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 3.45MRR4 pKa = 11.84YY5 pKa = 6.87TTMPVDD11 pKa = 3.9GVTLASYY18 pKa = 10.95SKK20 pKa = 11.09ALLPRR25 pKa = 11.84PQHH28 pKa = 6.03TGDD31 pKa = 3.33AGIDD35 pKa = 3.71LRR37 pKa = 11.84CASAFYY43 pKa = 10.71LEE45 pKa = 5.12PGEE48 pKa = 4.19TMQIGTGVVVDD59 pKa = 4.47NIDD62 pKa = 3.51RR63 pKa = 11.84YY64 pKa = 10.07IVGMVCSRR72 pKa = 11.84SGLAAHH78 pKa = 7.29DD79 pKa = 4.18GVHH82 pKa = 5.77VLNAPGIIDD91 pKa = 3.24SMYY94 pKa = 9.41RR95 pKa = 11.84GEE97 pKa = 4.22IAVILHH103 pKa = 6.0NASSHH108 pKa = 5.54RR109 pKa = 11.84CVVEE113 pKa = 3.8AGRR116 pKa = 11.84RR117 pKa = 11.84IAQLVFTPVCPTVAPVQDD135 pKa = 3.57TDD137 pKa = 3.58RR138 pKa = 11.84GEE140 pKa = 4.43GGLGSTDD147 pKa = 3.4SINPRR152 pKa = 11.84EE153 pKa = 4.26YY154 pKa = 10.74AVPQHH159 pKa = 5.75YY160 pKa = 10.85VIGDD164 pKa = 3.58AFDD167 pKa = 3.75GHH169 pKa = 7.56DD170 pKa = 5.22DD171 pKa = 4.36GADD174 pKa = 3.33DD175 pKa = 4.73AANLFEE181 pKa = 7.11ADD183 pKa = 5.23DD184 pKa = 4.36EE185 pKa = 4.53LLSDD189 pKa = 4.46EE190 pKa = 4.8DD191 pKa = 5.35RR192 pKa = 11.84EE193 pKa = 4.6IADD196 pKa = 4.6LLLGDD201 pKa = 4.78GDD203 pKa = 6.16DD204 pKa = 4.53MLTDD208 pKa = 4.91DD209 pKa = 5.57LPSDD213 pKa = 3.81MDD215 pKa = 4.15DD216 pKa = 4.63LVDD219 pKa = 5.17KK220 pKa = 10.21VTTLIFKK227 pKa = 10.35AAAANLNDD235 pKa = 5.36DD236 pKa = 3.97GTIPDD241 pKa = 4.95DD242 pKa = 3.7NAGSDD247 pKa = 4.21DD248 pKa = 4.08VVSHH252 pKa = 6.88PKK254 pKa = 10.14HH255 pKa = 5.2YY256 pKa = 10.45AANRR260 pKa = 11.84DD261 pKa = 3.78FPGLEE266 pKa = 4.35AILFTRR272 pKa = 11.84TMRR275 pKa = 11.84FGHH278 pKa = 6.04GNAVKK283 pKa = 10.14YY284 pKa = 10.0LWRR287 pKa = 11.84HH288 pKa = 5.06AYY290 pKa = 9.38KK291 pKa = 10.57GRR293 pKa = 11.84PVEE296 pKa = 4.27DD297 pKa = 3.21LRR299 pKa = 11.84KK300 pKa = 10.03ARR302 pKa = 11.84WYY304 pKa = 10.17LVNSIEE310 pKa = 4.91GGSQLLHH317 pKa = 6.76LKK319 pKa = 9.63NAPDD323 pKa = 3.39NADD326 pKa = 3.52NEE328 pKa = 4.55RR329 pKa = 11.84EE330 pKa = 4.03LLDD333 pKa = 4.01RR334 pKa = 11.84MDD336 pKa = 3.9YY337 pKa = 9.66MNYY340 pKa = 9.68MYY342 pKa = 10.09IEE344 pKa = 4.96CIGGRR349 pKa = 11.84GNKK352 pKa = 9.45HH353 pKa = 5.47EE354 pKa = 4.31ALKK357 pKa = 11.01DD358 pKa = 3.7VAKK361 pKa = 10.64LLAYY365 pKa = 9.82RR366 pKa = 11.84VMVGLIFYY374 pKa = 10.05GEE376 pKa = 4.34EE377 pKa = 3.76YY378 pKa = 10.73AALLRR383 pKa = 11.84GHH385 pKa = 6.4SLLGGDD391 pKa = 4.02TEE393 pKa = 5.49AMSEE397 pKa = 4.18TQLIRR402 pKa = 11.84ILDD405 pKa = 3.94CAIDD409 pKa = 3.76VYY411 pKa = 9.15TTLEE415 pKa = 3.95EE416 pKa = 4.11

Molecular weight: 45.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VPG7|A0A649VPG7_9CAUD Head-to-tail stopper OS=Corynebacterium phage EmiRose OX=2565372 GN=9 PE=4 SV=1
MM1 pKa = 7.0GTNDD5 pKa = 3.11VPGPGFIRR13 pKa = 11.84SGDD16 pKa = 3.54RR17 pKa = 11.84KK18 pKa = 9.87NKK20 pKa = 9.57ARR22 pKa = 11.84ASSWAWQRR30 pKa = 11.84RR31 pKa = 11.84VKK33 pKa = 10.78AEE35 pKa = 3.72RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84LEE40 pKa = 4.19KK41 pKa = 10.84DD42 pKa = 2.92PSLAVCWICNEE53 pKa = 4.68PIDD56 pKa = 3.84MTLPYY61 pKa = 10.1QHH63 pKa = 6.8ARR65 pKa = 11.84AFTLDD70 pKa = 3.21HH71 pKa = 6.63LVPIGRR77 pKa = 11.84GGALDD82 pKa = 4.05GDD84 pKa = 4.09TRR86 pKa = 11.84PAHH89 pKa = 6.4LSCNAKK95 pKa = 10.4RR96 pKa = 11.84GDD98 pKa = 3.28GRR100 pKa = 11.84RR101 pKa = 11.84KK102 pKa = 10.01KK103 pKa = 10.53KK104 pKa = 9.62EE105 pKa = 4.15SKK107 pKa = 8.32PTTIIGWW114 pKa = 3.57

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11873

60

886

258.1

27.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.657 ± 0.57

0.623 ± 0.097

6.73 ± 0.301

5.601 ± 0.329

2.577 ± 0.151

8.17 ± 0.431

1.583 ± 0.145

4.641 ± 0.221

4.54 ± 0.263

8.709 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.143

3.184 ± 0.214

5.55 ± 0.306

3.31 ± 0.212

5.938 ± 0.333

6.376 ± 0.328

6.831 ± 0.357

7.403 ± 0.24

1.752 ± 0.14

2.653 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski