Microbacterium phage Kelcole

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gillianvirus; unclassified Gillianvirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0A870|A0A6N0A870_9CAUD Uncharacterized protein OS=Microbacterium phage Kelcole OX=2744007 GN=87 PE=4 SV=1
MM1 pKa = 7.88AEE3 pKa = 3.63RR4 pKa = 11.84SYY6 pKa = 11.24EE7 pKa = 3.98RR8 pKa = 11.84NAGYY12 pKa = 10.76LSLTEE17 pKa = 4.82IPDD20 pKa = 3.4DD21 pKa = 3.8QYY23 pKa = 11.38IVGAVVVLMDD33 pKa = 3.89EE34 pKa = 4.33EE35 pKa = 5.21GNYY38 pKa = 8.39STVYY42 pKa = 10.63VNEE45 pKa = 4.47SLDD48 pKa = 3.82SVPDD52 pKa = 3.86PEE54 pKa = 4.38VQEE57 pKa = 4.21VQGPLIATAFSLANFGVRR75 pKa = 11.84PP76 pKa = 3.71

Molecular weight: 8.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0A4V3|A0A6N0A4V3_9CAUD Uncharacterized protein OS=Microbacterium phage Kelcole OX=2744007 GN=48 PE=4 SV=1
MM1 pKa = 7.49GSSRR5 pKa = 11.84DD6 pKa = 3.4ASGKK10 pKa = 9.28LRR12 pKa = 11.84RR13 pKa = 11.84GTSNSNDD20 pKa = 3.29RR21 pKa = 11.84GSAAEE26 pKa = 3.87RR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 10.08RR30 pKa = 11.84KK31 pKa = 9.5CWMLSWFGDD40 pKa = 4.7GIACLCYY47 pKa = 10.41SCGTVLLYY55 pKa = 9.82STLEE59 pKa = 3.79ADD61 pKa = 4.52RR62 pKa = 11.84IIPGVLGGTYY72 pKa = 10.46ARR74 pKa = 11.84GNIRR78 pKa = 11.84PSCGPCNRR86 pKa = 11.84RR87 pKa = 11.84GGNAVKK93 pKa = 10.54KK94 pKa = 10.39LIRR97 pKa = 11.84QKK99 pKa = 10.88VPLRR103 pKa = 11.84TILRR107 pKa = 11.84MCRR110 pKa = 11.84NGEE113 pKa = 3.99LL114 pKa = 3.6

Molecular weight: 12.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

19958

30

1061

190.1

20.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.249 ± 0.312

0.837 ± 0.108

6.173 ± 0.227

7.09 ± 0.414

2.751 ± 0.168

8.538 ± 0.261

1.824 ± 0.165

4.875 ± 0.176

3.252 ± 0.249

7.726 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.147

2.881 ± 0.16

5.542 ± 0.39

3.412 ± 0.282

6.739 ± 0.255

5.822 ± 0.37

7.095 ± 0.439

7.456 ± 0.263

1.949 ± 0.131

2.44 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski