Amino acid dipepetide frequency for Mycobacterium phage DrFeelGood

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.696AlaAla: 12.696 ± 1.052
0.769AlaCys: 0.769 ± 0.23
7.438AlaAsp: 7.438 ± 0.715
6.22AlaGlu: 6.22 ± 0.728
2.821AlaPhe: 2.821 ± 0.402
8.272AlaGly: 8.272 ± 0.878
1.347AlaHis: 1.347 ± 0.347
4.489AlaIle: 4.489 ± 0.554
4.168AlaLys: 4.168 ± 0.591
9.554AlaLeu: 9.554 ± 0.805
2.373AlaMet: 2.373 ± 0.411
2.693AlaAsn: 2.693 ± 0.408
5.066AlaPro: 5.066 ± 0.685
3.078AlaGln: 3.078 ± 0.412
5.963AlaArg: 5.963 ± 0.517
4.937AlaSer: 4.937 ± 0.552
5.579AlaThr: 5.579 ± 0.594
7.887AlaVal: 7.887 ± 0.714
1.667AlaTrp: 1.667 ± 0.337
2.95AlaTyr: 2.95 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.641CysAla: 0.641 ± 0.243
0.0CysCys: 0.0 ± 0.0
0.641CysAsp: 0.641 ± 0.227
0.769CysGlu: 0.769 ± 0.192
0.128CysPhe: 0.128 ± 0.084
0.385CysGly: 0.385 ± 0.162
0.192CysHis: 0.192 ± 0.117
0.256CysIle: 0.256 ± 0.133
0.192CysLys: 0.192 ± 0.114
0.449CysLeu: 0.449 ± 0.186
0.064CysMet: 0.064 ± 0.075
0.256CysAsn: 0.256 ± 0.129
0.192CysPro: 0.192 ± 0.112
0.192CysGln: 0.192 ± 0.099
0.321CysArg: 0.321 ± 0.14
0.256CysSer: 0.256 ± 0.132
0.385CysThr: 0.385 ± 0.145
0.192CysVal: 0.192 ± 0.1
0.256CysTrp: 0.256 ± 0.127
0.128CysTyr: 0.128 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
7.054AspAla: 7.054 ± 0.692
0.449AspCys: 0.449 ± 0.159
5.002AspAsp: 5.002 ± 0.546
3.463AspGlu: 3.463 ± 0.503
2.565AspPhe: 2.565 ± 0.367
5.899AspGly: 5.899 ± 0.607
1.347AspHis: 1.347 ± 0.319
2.757AspIle: 2.757 ± 0.388
2.757AspLys: 2.757 ± 0.494
7.567AspLeu: 7.567 ± 0.784
1.09AspMet: 1.09 ± 0.21
2.244AspAsn: 2.244 ± 0.359
4.809AspPro: 4.809 ± 0.618
1.411AspGln: 1.411 ± 0.35
3.527AspArg: 3.527 ± 0.38
3.206AspSer: 3.206 ± 0.494
3.783AspThr: 3.783 ± 0.355
4.617AspVal: 4.617 ± 0.495
1.86AspTrp: 1.86 ± 0.316
2.052AspTyr: 2.052 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
6.669GluAla: 6.669 ± 0.674
0.192GluCys: 0.192 ± 0.145
4.809GluAsp: 4.809 ± 0.5
5.13GluGlu: 5.13 ± 0.571
2.18GluPhe: 2.18 ± 0.401
3.719GluGly: 3.719 ± 0.424
1.475GluHis: 1.475 ± 0.374
3.847GluIle: 3.847 ± 0.536
2.373GluLys: 2.373 ± 0.428
6.797GluLeu: 6.797 ± 0.654
1.539GluMet: 1.539 ± 0.284
1.795GluAsn: 1.795 ± 0.408
2.629GluPro: 2.629 ± 0.424
2.565GluGln: 2.565 ± 0.355
3.719GluArg: 3.719 ± 0.565
3.014GluSer: 3.014 ± 0.37
3.591GluThr: 3.591 ± 0.539
5.258GluVal: 5.258 ± 0.574
1.795GluTrp: 1.795 ± 0.358
2.501GluTyr: 2.501 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.18PheAla: 2.18 ± 0.275
0.385PheCys: 0.385 ± 0.169
2.629PheAsp: 2.629 ± 0.35
2.18PheGlu: 2.18 ± 0.341
0.513PhePhe: 0.513 ± 0.212
3.655PheGly: 3.655 ± 0.515
0.834PheHis: 0.834 ± 0.241
1.539PheIle: 1.539 ± 0.293
1.411PheLys: 1.411 ± 0.32
2.501PheLeu: 2.501 ± 0.428
0.577PheMet: 0.577 ± 0.194
1.154PheAsn: 1.154 ± 0.283
1.795PhePro: 1.795 ± 0.313
1.154PheGln: 1.154 ± 0.249
1.86PheArg: 1.86 ± 0.422
1.475PheSer: 1.475 ± 0.277
2.18PheThr: 2.18 ± 0.383
1.924PheVal: 1.924 ± 0.362
0.577PheTrp: 0.577 ± 0.174
0.962PheTyr: 0.962 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
6.797GlyAla: 6.797 ± 1.007
0.513GlyCys: 0.513 ± 0.193
5.963GlyAsp: 5.963 ± 0.513
5.002GlyGlu: 5.002 ± 0.567
3.142GlyPhe: 3.142 ± 0.526
9.041GlyGly: 9.041 ± 2.072
2.052GlyHis: 2.052 ± 0.441
4.681GlyIle: 4.681 ± 0.674
3.399GlyLys: 3.399 ± 0.589
7.695GlyLeu: 7.695 ± 0.831
1.988GlyMet: 1.988 ± 0.292
3.399GlyAsn: 3.399 ± 0.404
4.04GlyPro: 4.04 ± 0.55
2.565GlyGln: 2.565 ± 0.335
4.681GlyArg: 4.681 ± 0.524
6.092GlySer: 6.092 ± 0.839
5.258GlyThr: 5.258 ± 0.65
5.322GlyVal: 5.322 ± 0.559
2.308GlyTrp: 2.308 ± 0.367
3.078GlyTyr: 3.078 ± 0.463
0.0GlyXaa: 0.0 ± 0.0
His
1.603HisAla: 1.603 ± 0.353
0.128HisCys: 0.128 ± 0.086
1.09HisAsp: 1.09 ± 0.241
1.411HisGlu: 1.411 ± 0.335
0.834HisPhe: 0.834 ± 0.197
1.603HisGly: 1.603 ± 0.366
0.577HisHis: 0.577 ± 0.174
0.898HisIle: 0.898 ± 0.233
1.026HisLys: 1.026 ± 0.284
1.475HisLeu: 1.475 ± 0.345
0.192HisMet: 0.192 ± 0.136
0.321HisAsn: 0.321 ± 0.133
1.282HisPro: 1.282 ± 0.258
1.026HisGln: 1.026 ± 0.241
1.411HisArg: 1.411 ± 0.318
0.641HisSer: 0.641 ± 0.166
1.026HisThr: 1.026 ± 0.295
1.795HisVal: 1.795 ± 0.328
0.449HisTrp: 0.449 ± 0.147
0.641HisTyr: 0.641 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
6.541IleAla: 6.541 ± 0.648
0.256IleCys: 0.256 ± 0.129
3.655IleAsp: 3.655 ± 0.436
3.591IleGlu: 3.591 ± 0.411
0.898IlePhe: 0.898 ± 0.227
3.655IleGly: 3.655 ± 0.424
0.898IleHis: 0.898 ± 0.234
1.924IleIle: 1.924 ± 0.338
1.731IleLys: 1.731 ± 0.352
3.206IleLeu: 3.206 ± 0.406
0.962IleMet: 0.962 ± 0.233
1.988IleAsn: 1.988 ± 0.298
3.27IlePro: 3.27 ± 0.368
1.218IleGln: 1.218 ± 0.242
3.655IleArg: 3.655 ± 0.44
3.27IleSer: 3.27 ± 0.538
4.168IleThr: 4.168 ± 0.462
2.565IleVal: 2.565 ± 0.457
0.577IleTrp: 0.577 ± 0.182
1.667IleTyr: 1.667 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
3.334LysAla: 3.334 ± 0.488
0.128LysCys: 0.128 ± 0.079
2.565LysAsp: 2.565 ± 0.506
2.244LysGlu: 2.244 ± 0.405
1.603LysPhe: 1.603 ± 0.308
2.501LysGly: 2.501 ± 0.337
1.218LysHis: 1.218 ± 0.255
2.693LysIle: 2.693 ± 0.506
1.86LysLys: 1.86 ± 0.408
3.014LysLeu: 3.014 ± 0.449
0.769LysMet: 0.769 ± 0.197
1.539LysAsn: 1.539 ± 0.32
2.886LysPro: 2.886 ± 0.473
1.603LysGln: 1.603 ± 0.317
3.014LysArg: 3.014 ± 0.472
2.373LysSer: 2.373 ± 0.452
2.437LysThr: 2.437 ± 0.354
3.527LysVal: 3.527 ± 0.558
0.834LysTrp: 0.834 ± 0.212
0.962LysTyr: 0.962 ± 0.276
0.0LysXaa: 0.0 ± 0.0
Leu
9.554LeuAla: 9.554 ± 0.864
0.256LeuCys: 0.256 ± 0.11
6.092LeuAsp: 6.092 ± 0.492
5.45LeuGlu: 5.45 ± 0.685
2.244LeuPhe: 2.244 ± 0.431
7.887LeuGly: 7.887 ± 0.671
1.347LeuHis: 1.347 ± 0.27
4.617LeuIle: 4.617 ± 0.568
3.719LeuLys: 3.719 ± 0.54
5.45LeuLeu: 5.45 ± 0.578
2.116LeuMet: 2.116 ± 0.34
3.142LeuAsn: 3.142 ± 0.459
5.707LeuPro: 5.707 ± 0.646
3.014LeuGln: 3.014 ± 0.489
5.963LeuArg: 5.963 ± 0.466
6.092LeuSer: 6.092 ± 0.729
5.45LeuThr: 5.45 ± 0.425
4.873LeuVal: 4.873 ± 0.709
1.026LeuTrp: 1.026 ± 0.264
2.373LeuTyr: 2.373 ± 0.411
0.0LeuXaa: 0.0 ± 0.0
Met
2.501MetAla: 2.501 ± 0.363
0.0MetCys: 0.0 ± 0.0
1.09MetAsp: 1.09 ± 0.238
1.603MetGlu: 1.603 ± 0.282
0.513MetPhe: 0.513 ± 0.155
1.475MetGly: 1.475 ± 0.311
0.321MetHis: 0.321 ± 0.146
0.898MetIle: 0.898 ± 0.276
0.962MetLys: 0.962 ± 0.226
1.539MetLeu: 1.539 ± 0.367
0.192MetMet: 0.192 ± 0.101
1.282MetAsn: 1.282 ± 0.249
1.026MetPro: 1.026 ± 0.235
0.705MetGln: 0.705 ± 0.219
1.347MetArg: 1.347 ± 0.287
1.924MetSer: 1.924 ± 0.321
1.924MetThr: 1.924 ± 0.277
1.154MetVal: 1.154 ± 0.335
0.192MetTrp: 0.192 ± 0.111
0.385MetTyr: 0.385 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.206AsnAla: 3.206 ± 0.485
0.128AsnCys: 0.128 ± 0.093
2.18AsnAsp: 2.18 ± 0.391
1.924AsnGlu: 1.924 ± 0.319
0.962AsnPhe: 0.962 ± 0.261
3.783AsnGly: 3.783 ± 0.519
0.641AsnHis: 0.641 ± 0.211
1.539AsnIle: 1.539 ± 0.318
0.834AsnLys: 0.834 ± 0.287
2.437AsnLeu: 2.437 ± 0.378
0.898AsnMet: 0.898 ± 0.232
1.026AsnAsn: 1.026 ± 0.265
3.014AsnPro: 3.014 ± 0.34
1.347AsnGln: 1.347 ± 0.37
1.282AsnArg: 1.282 ± 0.269
1.924AsnSer: 1.924 ± 0.395
2.116AsnThr: 2.116 ± 0.426
2.565AsnVal: 2.565 ± 0.429
0.834AsnTrp: 0.834 ± 0.207
1.218AsnTyr: 1.218 ± 0.282
0.0AsnXaa: 0.0 ± 0.0
Pro
5.45ProAla: 5.45 ± 0.51
0.321ProCys: 0.321 ± 0.144
4.36ProAsp: 4.36 ± 0.461
4.168ProGlu: 4.168 ± 0.439
2.308ProPhe: 2.308 ± 0.365
4.745ProGly: 4.745 ± 0.633
0.962ProHis: 0.962 ± 0.24
2.244ProIle: 2.244 ± 0.418
2.373ProLys: 2.373 ± 0.336
4.617ProLeu: 4.617 ± 0.504
1.154ProMet: 1.154 ± 0.28
1.667ProAsn: 1.667 ± 0.301
3.463ProPro: 3.463 ± 0.558
1.795ProGln: 1.795 ± 0.345
2.629ProArg: 2.629 ± 0.448
3.719ProSer: 3.719 ± 0.44
3.976ProThr: 3.976 ± 0.517
3.976ProVal: 3.976 ± 0.458
0.898ProTrp: 0.898 ± 0.307
1.475ProTyr: 1.475 ± 0.334
0.0ProXaa: 0.0 ± 0.0
Gln
3.591GlnAla: 3.591 ± 0.685
0.064GlnCys: 0.064 ± 0.061
1.347GlnAsp: 1.347 ± 0.378
1.539GlnGlu: 1.539 ± 0.25
1.282GlnPhe: 1.282 ± 0.237
2.886GlnGly: 2.886 ± 0.358
0.513GlnHis: 0.513 ± 0.162
2.308GlnIle: 2.308 ± 0.435
1.411GlnLys: 1.411 ± 0.364
3.976GlnLeu: 3.976 ± 0.537
0.705GlnMet: 0.705 ± 0.188
0.834GlnAsn: 0.834 ± 0.252
1.86GlnPro: 1.86 ± 0.298
1.924GlnGln: 1.924 ± 0.391
1.667GlnArg: 1.667 ± 0.398
1.795GlnSer: 1.795 ± 0.289
1.795GlnThr: 1.795 ± 0.321
2.437GlnVal: 2.437 ± 0.385
0.769GlnTrp: 0.769 ± 0.192
0.641GlnTyr: 0.641 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
5.258ArgAla: 5.258 ± 0.676
0.705ArgCys: 0.705 ± 0.249
2.886ArgAsp: 2.886 ± 0.441
4.873ArgGlu: 4.873 ± 0.527
1.603ArgPhe: 1.603 ± 0.347
5.322ArgGly: 5.322 ± 0.63
1.09ArgHis: 1.09 ± 0.288
2.886ArgIle: 2.886 ± 0.49
3.142ArgLys: 3.142 ± 0.453
5.835ArgLeu: 5.835 ± 0.804
1.86ArgMet: 1.86 ± 0.327
2.501ArgAsn: 2.501 ± 0.489
2.116ArgPro: 2.116 ± 0.316
1.667ArgGln: 1.667 ± 0.315
5.258ArgArg: 5.258 ± 0.634
3.527ArgSer: 3.527 ± 0.473
2.95ArgThr: 2.95 ± 0.468
5.194ArgVal: 5.194 ± 0.594
1.154ArgTrp: 1.154 ± 0.277
1.924ArgTyr: 1.924 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
6.284SerAla: 6.284 ± 0.725
0.385SerCys: 0.385 ± 0.153
3.719SerAsp: 3.719 ± 0.434
3.591SerGlu: 3.591 ± 0.44
1.667SerPhe: 1.667 ± 0.408
6.476SerGly: 6.476 ± 0.975
1.411SerHis: 1.411 ± 0.249
3.014SerIle: 3.014 ± 0.479
2.629SerLys: 2.629 ± 0.479
5.194SerLeu: 5.194 ± 0.668
1.347SerMet: 1.347 ± 0.263
2.116SerAsn: 2.116 ± 0.508
3.206SerPro: 3.206 ± 0.41
2.052SerGln: 2.052 ± 0.306
2.437SerArg: 2.437 ± 0.434
3.078SerSer: 3.078 ± 0.709
3.078SerThr: 3.078 ± 0.581
3.142SerVal: 3.142 ± 0.359
1.282SerTrp: 1.282 ± 0.361
1.09SerTyr: 1.09 ± 0.24
0.0SerXaa: 0.0 ± 0.0
Thr
6.156ThrAla: 6.156 ± 0.729
0.256ThrCys: 0.256 ± 0.148
4.104ThrAsp: 4.104 ± 0.561
4.296ThrGlu: 4.296 ± 0.553
2.18ThrPhe: 2.18 ± 0.313
6.541ThrGly: 6.541 ± 0.705
1.026ThrHis: 1.026 ± 0.279
2.373ThrIle: 2.373 ± 0.487
2.629ThrLys: 2.629 ± 0.308
5.45ThrLeu: 5.45 ± 0.68
0.834ThrMet: 0.834 ± 0.17
1.795ThrAsn: 1.795 ± 0.368
3.976ThrPro: 3.976 ± 0.492
1.86ThrGln: 1.86 ± 0.316
3.591ThrArg: 3.591 ± 0.614
3.27ThrSer: 3.27 ± 0.531
4.232ThrThr: 4.232 ± 0.51
5.771ThrVal: 5.771 ± 0.639
1.218ThrTrp: 1.218 ± 0.254
1.475ThrTyr: 1.475 ± 0.267
0.0ThrXaa: 0.0 ± 0.0
Val
6.412ValAla: 6.412 ± 0.661
0.449ValCys: 0.449 ± 0.19
5.13ValAsp: 5.13 ± 0.583
4.873ValGlu: 4.873 ± 0.594
2.437ValPhe: 2.437 ± 0.314
5.066ValGly: 5.066 ± 0.72
1.218ValHis: 1.218 ± 0.224
3.847ValIle: 3.847 ± 0.591
3.078ValLys: 3.078 ± 0.469
5.322ValLeu: 5.322 ± 0.561
1.411ValMet: 1.411 ± 0.316
2.437ValAsn: 2.437 ± 0.36
3.976ValPro: 3.976 ± 0.443
2.18ValGln: 2.18 ± 0.316
5.515ValArg: 5.515 ± 0.81
4.424ValSer: 4.424 ± 0.439
5.258ValThr: 5.258 ± 0.532
4.937ValVal: 4.937 ± 0.687
0.962ValTrp: 0.962 ± 0.258
2.116ValTyr: 2.116 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
1.539TrpAla: 1.539 ± 0.299
0.256TrpCys: 0.256 ± 0.117
1.539TrpAsp: 1.539 ± 0.3
0.898TrpGlu: 0.898 ± 0.221
0.898TrpPhe: 0.898 ± 0.235
1.667TrpGly: 1.667 ± 0.303
0.385TrpHis: 0.385 ± 0.164
1.218TrpIle: 1.218 ± 0.25
0.321TrpLys: 0.321 ± 0.151
1.731TrpLeu: 1.731 ± 0.283
0.256TrpMet: 0.256 ± 0.119
0.385TrpAsn: 0.385 ± 0.151
0.705TrpPro: 0.705 ± 0.258
0.769TrpGln: 0.769 ± 0.194
1.411TrpArg: 1.411 ± 0.323
0.898TrpSer: 0.898 ± 0.233
1.795TrpThr: 1.795 ± 0.366
1.988TrpVal: 1.988 ± 0.316
0.705TrpTrp: 0.705 ± 0.215
0.385TrpTyr: 0.385 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.501TyrAla: 2.501 ± 0.408
0.256TyrCys: 0.256 ± 0.149
1.218TyrAsp: 1.218 ± 0.344
2.373TyrGlu: 2.373 ± 0.321
0.705TyrPhe: 0.705 ± 0.199
2.437TyrGly: 2.437 ± 0.393
0.577TyrHis: 0.577 ± 0.191
1.667TyrIle: 1.667 ± 0.334
1.09TyrLys: 1.09 ± 0.256
2.629TyrLeu: 2.629 ± 0.451
0.641TyrMet: 0.641 ± 0.176
1.282TyrAsn: 1.282 ± 0.281
1.282TyrPro: 1.282 ± 0.27
1.218TyrGln: 1.218 ± 0.286
2.501TyrArg: 2.501 ± 0.342
1.282TyrSer: 1.282 ± 0.253
2.052TyrThr: 2.052 ± 0.384
1.924TyrVal: 1.924 ± 0.314
0.385TyrTrp: 0.385 ± 0.158
0.641TyrTyr: 0.641 ± 0.209
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (15596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski