Amino acid dipepetide frequency for Tetrahymena thermophila (strain SB210)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.423AlaAla: 1.423 ± 0.017	0.61AlaCys: 0.61 ± 0.011	1.403AlaAsp: 1.403 ± 0.011	1.686AlaGlu: 1.686 ± 0.015	1.777AlaPhe: 1.777 ± 0.013	1.151AlaGly: 1.151 ± 0.014	0.506AlaHis: 0.506 ± 0.006	2.847AlaIle: 2.847 ± 0.017	2.679AlaLys: 2.679 ± 0.018	3.399AlaLeu: 3.399 ± 0.019	0.559AlaMet: 0.559 ± 0.008	2.775AlaAsn: 2.775 ± 0.016	0.826AlaPro: 0.826 ± 0.009	2.775AlaGln: 2.775 ± 0.016	0.846AlaArg: 0.846 ± 0.008	2.882AlaSer: 2.882 ± 0.022	1.537AlaThr: 1.537 ± 0.012	1.432AlaVal: 1.432 ± 0.012	0.167AlaTrp: 0.167 ± 0.003	1.361AlaTyr: 1.361 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	0.57CysAla: 0.57 ± 0.01	0.265CysCys: 0.265 ± 0.006	0.935CysAsp: 0.935 ± 0.018	0.766CysGlu: 0.766 ± 0.009	0.943CysPhe: 0.943 ± 0.008	0.618CysGly: 0.618 ± 0.01	0.257CysHis: 0.257 ± 0.007	1.597CysIle: 1.597 ± 0.022	1.4CysLys: 1.4 ± 0.023	1.935CysLeu: 1.935 ± 0.024	0.282CysMet: 0.282 ± 0.004	1.225CysAsn: 1.225 ± 0.019	0.674CysPro: 0.674 ± 0.014	1.666CysGln: 1.666 ± 0.026	0.404CysArg: 0.404 ± 0.006	1.628CysSer: 1.628 ± 0.022	1.027CysThr: 1.027 ± 0.018	0.844CysVal: 0.844 ± 0.015	0.091CysTrp: 0.091 ± 0.002	0.746CysTyr: 0.746 ± 0.009	0.001CysXaa: 0.001 ± 0.0
Asp	1.336AspAla: 1.336 ± 0.01	0.838AspCys: 0.838 ± 0.012	2.755AspAsp: 2.755 ± 0.021	3.682AspGlu: 3.682 ± 0.025	2.826AspPhe: 2.826 ± 0.014	1.729AspGly: 1.729 ± 0.018	0.686AspHis: 0.686 ± 0.007	4.22AspIle: 4.22 ± 0.02	3.969AspLys: 3.969 ± 0.021	4.874AspLeu: 4.874 ± 0.022	0.825AspMet: 0.825 ± 0.008	3.564AspAsn: 3.564 ± 0.017	1.377AspPro: 1.377 ± 0.013	4.547AspGln: 4.547 ± 0.021	1.226AspArg: 1.226 ± 0.009	3.838AspSer: 3.838 ± 0.018	1.811AspThr: 1.811 ± 0.013	1.793AspVal: 1.793 ± 0.012	0.288AspTrp: 0.288 ± 0.005	2.254AspTyr: 2.254 ± 0.012	0.0AspXaa: 0.0 ± 0.0
Glu	1.89GluAla: 1.89 ± 0.014	0.875GluCys: 0.875 ± 0.011	3.456GluAsp: 3.456 ± 0.024	5.586GluGlu: 5.586 ± 0.041	2.644GluPhe: 2.644 ± 0.015	2.095GluGly: 2.095 ± 0.017	0.706GluHis: 0.706 ± 0.006	5.422GluIle: 5.422 ± 0.026	6.223GluLys: 6.223 ± 0.036	4.741GluLeu: 4.741 ± 0.024	1.168GluMet: 1.168 ± 0.01	5.582GluAsn: 5.582 ± 0.026	0.881GluPro: 0.881 ± 0.009	5.323GluGln: 5.323 ± 0.032	1.872GluArg: 1.872 ± 0.013	3.9GluSer: 3.9 ± 0.02	2.19GluThr: 2.19 ± 0.013	2.381GluVal: 2.381 ± 0.014	0.296GluTrp: 0.296 ± 0.005	2.37GluTyr: 2.37 ± 0.012	0.001GluXaa: 0.001 ± 0.0
Phe	1.684PheAla: 1.684 ± 0.012	0.901PheCys: 0.901 ± 0.009	2.783PheAsp: 2.783 ± 0.014	2.978PheGlu: 2.978 ± 0.016	2.851PhePhe: 2.851 ± 0.019	1.994PheGly: 1.994 ± 0.014	0.715PheHis: 0.715 ± 0.007	4.39PheIle: 4.39 ± 0.025	4.168PheLys: 4.168 ± 0.018	4.858PheLeu: 4.858 ± 0.024	1.025PheMet: 1.025 ± 0.008	4.227PheAsn: 4.227 ± 0.019	1.169PhePro: 1.169 ± 0.011	4.575PheGln: 4.575 ± 0.021	1.349PheArg: 1.349 ± 0.011	4.513PheSer: 4.513 ± 0.022	2.414PheThr: 2.414 ± 0.017	2.174PheVal: 2.174 ± 0.015	0.33PheTrp: 0.33 ± 0.005	2.695PheTyr: 2.695 ± 0.017	0.002PheXaa: 0.002 ± 0.0
Gly	1.763GlyAla: 1.763 ± 0.026	0.657GlyCys: 0.657 ± 0.01	1.85GlyAsp: 1.85 ± 0.016	1.795GlyGlu: 1.795 ± 0.014	2.041GlyPhe: 2.041 ± 0.014	1.702GlyGly: 1.702 ± 0.019	0.466GlyHis: 0.466 ± 0.007	2.838GlyIle: 2.838 ± 0.018	2.431GlyLys: 2.431 ± 0.017	3.345GlyLeu: 3.345 ± 0.031	0.622GlyMet: 0.622 ± 0.007	2.633GlyAsn: 2.633 ± 0.02	0.68GlyPro: 0.68 ± 0.011	2.594GlyGln: 2.594 ± 0.016	0.9GlyArg: 0.9 ± 0.009	3.018GlySer: 3.018 ± 0.025	1.577GlyThr: 1.577 ± 0.016	1.717GlyVal: 1.717 ± 0.013	0.262GlyTrp: 0.262 ± 0.007	1.78GlyTyr: 1.78 ± 0.019	0.001GlyXaa: 0.001 ± 0.0
His	0.407HisAla: 0.407 ± 0.006	0.179HisCys: 0.179 ± 0.004	0.567HisAsp: 0.567 ± 0.008	0.764HisGlu: 0.764 ± 0.007	0.908HisPhe: 0.908 ± 0.009	0.436HisGly: 0.436 ± 0.006	0.388HisHis: 0.388 ± 0.007	1.191HisIle: 1.191 ± 0.008	1.164HisLys: 1.164 ± 0.01	1.564HisLeu: 1.564 ± 0.011	0.247HisMet: 0.247 ± 0.004	1.13HisAsn: 1.13 ± 0.01	0.51HisPro: 0.51 ± 0.007	1.395HisGln: 1.395 ± 0.01	0.448HisArg: 0.448 ± 0.006	1.281HisSer: 1.281 ± 0.009	0.616HisThr: 0.616 ± 0.007	0.491HisVal: 0.491 ± 0.006	0.08HisTrp: 0.08 ± 0.002	0.65HisTyr: 0.65 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	2.779IleAla: 2.779 ± 0.016	1.504IleCys: 1.504 ± 0.017	4.5IleAsp: 4.5 ± 0.019	5.062IleGlu: 5.062 ± 0.02	4.798IlePhe: 4.798 ± 0.029	3.246IleGly: 3.246 ± 0.031	1.274IleHis: 1.274 ± 0.009	7.023IleIle: 7.023 ± 0.034	7.495IleLys: 7.495 ± 0.027	7.885IleLeu: 7.885 ± 0.03	1.42IleMet: 1.42 ± 0.008	7.792IleAsn: 7.792 ± 0.026	2.463IlePro: 2.463 ± 0.015	8.377IleGln: 8.377 ± 0.033	2.2IleArg: 2.2 ± 0.012	7.081IleSer: 7.081 ± 0.027	3.655IleThr: 3.655 ± 0.017	3.445IleVal: 3.445 ± 0.018	0.472IleTrp: 0.472 ± 0.007	3.85IleTyr: 3.85 ± 0.024	0.002IleXaa: 0.002 ± 0.0
Lys	2.697LysAla: 2.697 ± 0.016	1.728LysCys: 1.728 ± 0.028	4.295LysAsp: 4.295 ± 0.021	6.024LysGlu: 6.024 ± 0.034	3.761LysPhe: 3.761 ± 0.016	2.604LysGly: 2.604 ± 0.016	1.173LysHis: 1.173 ± 0.01	7.999LysIle: 7.999 ± 0.029	9.023LysLys: 9.023 ± 0.045	7.555LysLeu: 7.555 ± 0.026	1.799LysMet: 1.799 ± 0.01	7.979LysAsn: 7.979 ± 0.034	1.878LysPro: 1.878 ± 0.012	8.578LysGln: 8.578 ± 0.045	2.79LysArg: 2.79 ± 0.017	6.411LysSer: 6.411 ± 0.028	3.643LysThr: 3.643 ± 0.018	3.454LysVal: 3.454 ± 0.017	0.41LysTrp: 0.41 ± 0.005	3.759LysTyr: 3.759 ± 0.016	0.003LysXaa: 0.003 ± 0.0
Leu	3.419LeuAla: 3.419 ± 0.022	1.347LeuCys: 1.347 ± 0.013	4.637LeuAsp: 4.637 ± 0.017	5.538LeuGlu: 5.538 ± 0.029	4.679LeuPhe: 4.679 ± 0.027	3.491LeuGly: 3.491 ± 0.031	1.315LeuHis: 1.315 ± 0.011	7.798LeuIle: 7.798 ± 0.033	8.826LeuLys: 8.826 ± 0.03	7.659LeuLeu: 7.659 ± 0.032	1.627LeuMet: 1.627 ± 0.012	8.592LeuAsn: 8.592 ± 0.029	2.266LeuPro: 2.266 ± 0.013	7.797LeuGln: 7.797 ± 0.031	2.711LeuArg: 2.711 ± 0.014	7.953LeuSer: 7.953 ± 0.035	4.407LeuThr: 4.407 ± 0.027	3.858LeuVal: 3.858 ± 0.018	0.446LeuTrp: 0.446 ± 0.006	3.525LeuTyr: 3.525 ± 0.017	0.002LeuXaa: 0.002 ± 0.0
Met	0.605MetAla: 0.605 ± 0.006	0.243MetCys: 0.243 ± 0.004	0.826MetAsp: 0.826 ± 0.008	1.03MetGlu: 1.03 ± 0.008	0.758MetPhe: 0.758 ± 0.007	0.625MetGly: 0.625 ± 0.008	0.317MetHis: 0.317 ± 0.005	1.707MetIle: 1.707 ± 0.01	1.857MetLys: 1.857 ± 0.013	1.507MetLeu: 1.507 ± 0.011	0.456MetMet: 0.456 ± 0.005	1.762MetAsn: 1.762 ± 0.011	0.458MetPro: 0.458 ± 0.006	1.457MetGln: 1.457 ± 0.009	0.643MetArg: 0.643 ± 0.006	1.391MetSer: 1.391 ± 0.009	0.771MetThr: 0.771 ± 0.007	0.738MetVal: 0.738 ± 0.007	0.088MetTrp: 0.088 ± 0.002	0.642MetTyr: 0.642 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.252AsnAla: 2.252 ± 0.014	1.846AsnCys: 1.846 ± 0.025	3.579AsnAsp: 3.579 ± 0.017	4.797AsnGlu: 4.797 ± 0.022	4.478AsnPhe: 4.478 ± 0.018	2.759AsnGly: 2.759 ± 0.022	1.356AsnHis: 1.356 ± 0.011	7.647AsnIle: 7.647 ± 0.026	7.426AsnLys: 7.426 ± 0.031	9.549AsnLeu: 9.549 ± 0.047	1.588AsnMet: 1.588 ± 0.01	9.478AsnAsn: 9.478 ± 0.06	2.578AsnPro: 2.578 ± 0.017	10.804AsnGln: 10.804 ± 0.048	2.124AsnArg: 2.124 ± 0.014	8.149AsnSer: 8.149 ± 0.038	4.051AsnThr: 4.051 ± 0.023	2.86AsnVal: 2.86 ± 0.015	0.38AsnTrp: 0.38 ± 0.005	4.093AsnTyr: 4.093 ± 0.018	0.001AsnXaa: 0.001 ± 0.0
Pro	0.86ProAla: 0.86 ± 0.01	0.402ProCys: 0.402 ± 0.009	1.111ProAsp: 1.111 ± 0.011	1.384ProGlu: 1.384 ± 0.012	1.37ProPhe: 1.37 ± 0.012	0.743ProGly: 0.743 ± 0.01	0.43ProHis: 0.43 ± 0.006	2.144ProIle: 2.144 ± 0.014	2.084ProLys: 2.084 ± 0.016	2.313ProLeu: 2.313 ± 0.014	0.341ProMet: 0.341 ± 0.005	2.317ProAsn: 2.317 ± 0.019	0.997ProPro: 0.997 ± 0.016	2.591ProGln: 2.591 ± 0.018	0.632ProArg: 0.632 ± 0.007	2.487ProSer: 2.487 ± 0.019	1.374ProThr: 1.374 ± 0.014	1.089ProVal: 1.089 ± 0.009	0.127ProTrp: 0.127 ± 0.003	1.129ProTyr: 1.129 ± 0.01	0.0ProXaa: 0.0 ± 0.0
Gln	2.855GlnAla: 2.855 ± 0.014	1.472GlnCys: 1.472 ± 0.028	4.061GlnAsp: 4.061 ± 0.018	5.534GlnGlu: 5.534 ± 0.031	4.284GlnPhe: 4.284 ± 0.019	2.797GlnGly: 2.797 ± 0.018	1.205GlnHis: 1.205 ± 0.011	9.488GlnIle: 9.488 ± 0.038	9.023GlnLys: 9.023 ± 0.045	7.619GlnLeu: 7.619 ± 0.032	1.858GlnMet: 1.858 ± 0.011	11.103GlnAsn: 11.103 ± 0.05	2.037GlnPro: 2.037 ± 0.018	11.433GlnGln: 11.433 ± 0.09	2.452GlnArg: 2.452 ± 0.014	7.355GlnSer: 7.355 ± 0.042	4.156GlnThr: 4.156 ± 0.018	3.584GlnVal: 3.584 ± 0.016	0.303GlnTrp: 0.303 ± 0.004	3.583GlnTyr: 3.583 ± 0.02	0.002GlnXaa: 0.002 ± 0.0
Arg	0.935ArgAla: 0.935 ± 0.009	0.41ArgCys: 0.41 ± 0.007	1.414ArgAsp: 1.414 ± 0.01	1.751ArgGlu: 1.751 ± 0.013	1.351ArgPhe: 1.351 ± 0.009	0.92ArgGly: 0.92 ± 0.008	0.403ArgHis: 0.403 ± 0.006	2.377ArgIle: 2.377 ± 0.013	2.682ArgLys: 2.682 ± 0.017	2.382ArgLeu: 2.382 ± 0.014	0.549ArgMet: 0.549 ± 0.006	2.321ArgAsn: 2.321 ± 0.013	0.64ArgPro: 0.64 ± 0.008	2.268ArgGln: 2.268 ± 0.015	1.072ArgArg: 1.072 ± 0.01	2.019ArgSer: 2.019 ± 0.014	1.019ArgThr: 1.019 ± 0.008	1.283ArgVal: 1.283 ± 0.009	0.15ArgTrp: 0.15 ± 0.003	1.155ArgTyr: 1.155 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	2.656SerAla: 2.656 ± 0.018	1.755SerCys: 1.755 ± 0.033	3.716SerAsp: 3.716 ± 0.014	3.979SerGlu: 3.979 ± 0.021	4.422SerPhe: 4.422 ± 0.02	2.942SerGly: 2.942 ± 0.028	1.22SerHis: 1.22 ± 0.01	6.772SerIle: 6.772 ± 0.028	6.731SerLys: 6.731 ± 0.031	7.523SerLeu: 7.523 ± 0.028	1.271SerMet: 1.271 ± 0.01	8.111SerAsn: 8.111 ± 0.036	2.56SerPro: 2.56 ± 0.022	8.483SerGln: 8.483 ± 0.044	2.036SerArg: 2.036 ± 0.013	8.288SerSer: 8.288 ± 0.043	3.847SerThr: 3.847 ± 0.02	3.122SerVal: 3.122 ± 0.016	0.347SerTrp: 0.347 ± 0.005	3.367SerTyr: 3.367 ± 0.018	0.002SerXaa: 0.002 ± 0.0
Thr	1.552ThrAla: 1.552 ± 0.012	1.176ThrCys: 1.176 ± 0.03	1.957ThrAsp: 1.957 ± 0.013	2.171ThrGlu: 2.171 ± 0.014	2.448ThrPhe: 2.448 ± 0.015	1.528ThrGly: 1.528 ± 0.015	0.7ThrHis: 0.7 ± 0.007	3.643ThrIle: 3.643 ± 0.018	3.403ThrLys: 3.403 ± 0.015	4.373ThrLeu: 4.373 ± 0.025	0.624ThrMet: 0.624 ± 0.007	4.068ThrAsn: 4.068 ± 0.024	1.587ThrPro: 1.587 ± 0.015	3.947ThrGln: 3.947 ± 0.018	0.98ThrArg: 0.98 ± 0.009	3.757ThrSer: 3.757 ± 0.02	2.198ThrThr: 2.198 ± 0.019	1.813ThrVal: 1.813 ± 0.013	0.221ThrTrp: 0.221 ± 0.004	1.844ThrTyr: 1.844 ± 0.014	0.001ThrXaa: 0.001 ± 0.0
Val	1.485ValAla: 1.485 ± 0.011	0.801ValCys: 0.801 ± 0.011	2.11ValAsp: 2.11 ± 0.012	2.347ValGlu: 2.347 ± 0.014	2.29ValPhe: 2.29 ± 0.015	1.545ValGly: 1.545 ± 0.012	0.552ValHis: 0.552 ± 0.006	3.319ValIle: 3.319 ± 0.017	3.364ValLys: 3.364 ± 0.016	3.646ValLeu: 3.646 ± 0.017	0.717ValMet: 0.717 ± 0.007	3.33ValAsn: 3.33 ± 0.016	1.056ValPro: 1.056 ± 0.012	3.268ValGln: 3.268 ± 0.017	1.107ValArg: 1.107 ± 0.009	3.267ValSer: 3.267 ± 0.017	1.686ValThr: 1.686 ± 0.014	1.892ValVal: 1.892 ± 0.013	0.268ValTrp: 0.268 ± 0.004	1.794ValTyr: 1.794 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.193TrpAla: 0.193 ± 0.004	0.073TrpCys: 0.073 ± 0.002	0.304TrpAsp: 0.304 ± 0.005	0.296TrpGlu: 0.296 ± 0.004	0.237TrpPhe: 0.237 ± 0.004	0.229TrpGly: 0.229 ± 0.004	0.073TrpHis: 0.073 ± 0.002	0.481TrpIle: 0.481 ± 0.006	0.442TrpLys: 0.442 ± 0.005	0.421TrpLeu: 0.421 ± 0.006	0.126TrpMet: 0.126 ± 0.003	0.44TrpAsn: 0.44 ± 0.007	0.122TrpPro: 0.122 ± 0.003	0.295TrpGln: 0.295 ± 0.004	0.215TrpArg: 0.215 ± 0.004	0.38TrpSer: 0.38 ± 0.005	0.22TrpThr: 0.22 ± 0.005	0.272TrpVal: 0.272 ± 0.005	0.053TrpTrp: 0.053 ± 0.002	0.203TrpTyr: 0.203 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.281TyrAla: 1.281 ± 0.01	0.792TyrCys: 0.792 ± 0.012	2.177TyrAsp: 2.177 ± 0.013	2.432TyrGlu: 2.432 ± 0.012	2.926TyrPhe: 2.926 ± 0.018	1.519TyrGly: 1.519 ± 0.013	0.674TyrHis: 0.674 ± 0.007	3.418TyrIle: 3.418 ± 0.018	3.17TyrLys: 3.17 ± 0.015	4.741TyrLeu: 4.741 ± 0.022	0.778TyrMet: 0.778 ± 0.007	3.365TyrAsn: 3.365 ± 0.018	1.166TyrPro: 1.166 ± 0.01	4.028TyrGln: 4.028 ± 0.019	1.092TyrArg: 1.092 ± 0.008	3.552TyrSer: 3.552 ± 0.019	1.793TyrThr: 1.793 ± 0.013	1.603TyrVal: 1.603 ± 0.012	0.294TyrTrp: 0.294 ± 0.005	2.336TyrTyr: 2.336 ± 0.016	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.002XaaLeu: 0.002 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.655XaaXaa: 0.655 ± 0.037

Statistics based on 26976 proteins (16915139 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---