Zhengella mangrovi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Zhengella

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G1QGF1|A0A2G1QGF1_9RHIZ Uncharacterized protein (Fragment) OS=Zhengella mangrovi OX=1982044 GN=CSC94_23945 PE=3 SV=1
MM1 pKa = 8.22RR2 pKa = 11.84IPCKK6 pKa = 10.09AWRR9 pKa = 11.84KK10 pKa = 9.91AILCVVTALGVFQAEE25 pKa = 4.48SSAVAADD32 pKa = 3.87TCTSAPTTFNTATGRR47 pKa = 11.84VTDD50 pKa = 3.96GTYY53 pKa = 10.21SASYY57 pKa = 9.84SYY59 pKa = 11.14SILQSAGYY67 pKa = 9.49GAATPAGADD76 pKa = 3.57GLVLDD81 pKa = 4.72VANGAANPDD90 pKa = 4.54SITVRR95 pKa = 11.84YY96 pKa = 8.57NVSGVTSPGHH106 pKa = 5.65VIRR109 pKa = 11.84LVGGSFNTVNGDD121 pKa = 3.38NAASDD126 pKa = 4.15YY127 pKa = 9.94TISWTGGTGSATWYY141 pKa = 10.51DD142 pKa = 3.82PATPDD147 pKa = 2.87VTMYY151 pKa = 10.42RR152 pKa = 11.84WAPPAGFDD160 pKa = 3.23INEE163 pKa = 4.05RR164 pKa = 11.84QIEE167 pKa = 4.21GLGTSGTLANGGTIRR182 pKa = 11.84VYY184 pKa = 10.71DD185 pKa = 3.44VSNAVTEE192 pKa = 4.64WYY194 pKa = 10.68VDD196 pKa = 3.8LPRR199 pKa = 11.84GATSVTVSKK208 pKa = 11.01VVLHH212 pKa = 6.79GGTSATTGIDD222 pKa = 3.45FQYY225 pKa = 10.45PALGYY230 pKa = 9.94GSSGSGTSFRR240 pKa = 11.84EE241 pKa = 4.06WIAFDD246 pKa = 3.67TLLCRR251 pKa = 11.84DD252 pKa = 3.66TDD254 pKa = 4.04LVTVKK259 pKa = 9.83TLEE262 pKa = 4.42SGNATPSVGDD272 pKa = 3.43TVGFRR277 pKa = 11.84INVVNNGPDD286 pKa = 3.05AATNVALTDD295 pKa = 3.62QVPAGLTFVSATPGAGGYY313 pKa = 9.46DD314 pKa = 3.51AATGIWTIGTLANGASASLVLTATVNADD342 pKa = 3.1QGGQGITNTTTPAAGDD358 pKa = 3.35QGDD361 pKa = 4.25PSTAGDD367 pKa = 4.54DD368 pKa = 3.69LTEE371 pKa = 5.41SIMVLAQPDD380 pKa = 4.1LVTVKK385 pKa = 10.8SLLSANATPAVGDD398 pKa = 3.89SVTFRR403 pKa = 11.84LMVTNATAGVTATGVSLNDD422 pKa = 3.41QMPVGLTYY430 pKa = 10.8ASHH433 pKa = 5.94VASAGTYY440 pKa = 10.53DD441 pKa = 4.03PATGAWTIGSVVNGTPATLDD461 pKa = 2.9ITATVDD467 pKa = 3.57AGQQGNLITNTATPATGDD485 pKa = 3.46QPDD488 pKa = 3.93PSMLGDD494 pKa = 4.39DD495 pKa = 4.17LMEE498 pKa = 4.44SVTVFNPQPALVLAKK513 pKa = 8.86TWSFVTDD520 pKa = 3.64ANGDD524 pKa = 3.83GKK526 pKa = 10.79AGTGDD531 pKa = 3.62VVRR534 pKa = 11.84YY535 pKa = 9.48AYY537 pKa = 10.48AVTNTGNVNVANVSVSDD554 pKa = 3.71TTNGNDD560 pKa = 3.41PAFLGGASPGQPVAVSLTTDD580 pKa = 2.98AGTVGDD586 pKa = 4.84STDD589 pKa = 3.73ADD591 pKa = 3.62NAGPVWDD598 pKa = 4.55TLAPGDD604 pKa = 4.24TVTFTADD611 pKa = 3.42YY612 pKa = 10.79SVVQADD618 pKa = 3.33VDD620 pKa = 3.88ALQQ623 pKa = 3.87

Molecular weight: 62.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G1QHA3|A0A2G1QHA3_9RHIZ Uncharacterized protein OS=Zhengella mangrovi OX=1982044 GN=CSC94_22370 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.47GGRR28 pKa = 11.84KK29 pKa = 9.23VIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.99

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4686

0

4686

1491495

29

2686

318.3

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.742 ± 0.056

0.86 ± 0.012

5.979 ± 0.032

5.641 ± 0.031

3.808 ± 0.022

8.909 ± 0.035

2.057 ± 0.018

5.205 ± 0.026

3.205 ± 0.028

9.696 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.796 ± 0.018

2.591 ± 0.02

5.057 ± 0.025

2.871 ± 0.017

7.098 ± 0.041

5.265 ± 0.026

5.329 ± 0.03

7.384 ± 0.026

1.333 ± 0.014

2.176 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski