Nitrospira lenta

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A330L957|A0A330L957_9BACT Elongation factor G (EF-G) OS=Nitrospira lenta OX=1436998 GN=fusA PE=3 SV=1
MM1 pKa = 8.19RR2 pKa = 11.84YY3 pKa = 9.9LFLPSDD9 pKa = 3.81MLDD12 pKa = 3.57DD13 pKa = 4.69LGVVVLVIFYY23 pKa = 10.83

Molecular weight: 2.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A330LBJ5|A0A330LBJ5_9BACT Uncharacterized protein OS=Nitrospira lenta OX=1436998 GN=NITLEN_70056 PE=4 SV=1
MM1 pKa = 7.59LPRR4 pKa = 11.84VAKK7 pKa = 10.06AVPRR11 pKa = 11.84QEE13 pKa = 3.72PRR15 pKa = 11.84VFQSLRR21 pKa = 11.84IGLASLTKK29 pKa = 9.85RR30 pKa = 11.84WKK32 pKa = 9.68EE33 pKa = 3.67SQGAGKK39 pKa = 9.54QCAATAHH46 pKa = 6.64AIIALVPEE54 pKa = 4.45PPGLRR59 pKa = 11.84VIEE62 pKa = 4.85GKK64 pKa = 10.72LSTASYY70 pKa = 9.41TYY72 pKa = 10.31VAPFPCPSITRR83 pKa = 11.84NRR85 pKa = 11.84RR86 pKa = 11.84SKK88 pKa = 11.08SSWIYY93 pKa = 9.59CARR96 pKa = 11.84SATARR101 pKa = 11.84VDD103 pKa = 3.92SNDD106 pKa = 2.93

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3885

0

3885

1139029

16

3260

293.2

32.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.062 ± 0.041

1.006 ± 0.014

5.114 ± 0.03

5.812 ± 0.04

3.546 ± 0.026

7.875 ± 0.04

2.377 ± 0.02

5.243 ± 0.031

4.037 ± 0.039

10.552 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.471 ± 0.019

2.63 ± 0.024

5.159 ± 0.034

4.102 ± 0.026

6.732 ± 0.041

5.955 ± 0.034

5.8 ± 0.037

7.677 ± 0.04

1.345 ± 0.02

2.505 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski