Gordonia phage Aphelion

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Smoothievirus; unclassified Smoothievirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410TD77|A0A410TD77_9CAUD Uncharacterized protein OS=Gordonia phage Aphelion OX=2507860 GN=111 PE=4 SV=1
MM1 pKa = 7.16TAQGMTIYY9 pKa = 10.73EE10 pKa = 4.44PFTLGRR16 pKa = 11.84LIKK19 pKa = 10.15NLRR22 pKa = 11.84DD23 pKa = 3.73LPPDD27 pKa = 3.51TEE29 pKa = 4.28VRR31 pKa = 11.84GLDD34 pKa = 3.77GMVHH38 pKa = 6.79SYY40 pKa = 10.53RR41 pKa = 11.84GWYY44 pKa = 8.08EE45 pKa = 4.07RR46 pKa = 11.84NATTPTGLTKK56 pKa = 10.09TAGEE60 pKa = 4.13LADD63 pKa = 5.62FYY65 pKa = 11.19QADD68 pKa = 3.7VGEE71 pKa = 4.67TMGGWKK77 pKa = 10.39GGDD80 pKa = 3.65YY81 pKa = 10.37IISADD86 pKa = 3.64QLIYY90 pKa = 10.41YY91 pKa = 9.95ADD93 pKa = 3.84YY94 pKa = 11.48GDD96 pKa = 4.64IGPLIIGLEE105 pKa = 4.09PTDD108 pKa = 5.17DD109 pKa = 4.66DD110 pKa = 4.04GTYY113 pKa = 10.33TPVLLVDD120 pKa = 3.75NNYY123 pKa = 10.81LLL125 pKa = 4.64

Molecular weight: 13.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410TCZ2|A0A410TCZ2_9CAUD Uncharacterized protein OS=Gordonia phage Aphelion OX=2507860 GN=11 PE=4 SV=1
MM1 pKa = 7.39PRR3 pKa = 11.84SRR5 pKa = 11.84SWSDD9 pKa = 3.05DD10 pKa = 3.38DD11 pKa = 4.9LRR13 pKa = 11.84AAVRR17 pKa = 11.84SSQTYY22 pKa = 10.19ADD24 pKa = 3.69VARR27 pKa = 11.84ALSMHH32 pKa = 6.94PGSSTVGRR40 pKa = 11.84LRR42 pKa = 11.84DD43 pKa = 3.46RR44 pKa = 11.84TRR46 pKa = 11.84ALGLDD51 pKa = 3.31TRR53 pKa = 11.84HH54 pKa = 5.64FTRR57 pKa = 11.84ARR59 pKa = 11.84PRR61 pKa = 11.84EE62 pKa = 3.81DD63 pKa = 2.83MFRR66 pKa = 11.84SGVRR70 pKa = 11.84YY71 pKa = 7.38HH72 pKa = 6.2TGMRR76 pKa = 11.84DD77 pKa = 2.94RR78 pKa = 11.84YY79 pKa = 8.64LQLMPYY85 pKa = 8.75EE86 pKa = 4.24CQICGVSKK94 pKa = 10.15WCGQPLTLQLDD105 pKa = 4.43HH106 pKa = 7.59IDD108 pKa = 3.63GDD110 pKa = 4.0RR111 pKa = 11.84MNNTLQNLRR120 pKa = 11.84LLCPNCHH127 pKa = 6.03SQTATWSRR135 pKa = 11.84KK136 pKa = 8.97KK137 pKa = 10.77SNPVV141 pKa = 2.8

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

176

0

176

29136

38

2931

165.5

18.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.572 ± 0.369

1.061 ± 0.144

7.19 ± 0.195

6.772 ± 0.187

3.195 ± 0.101

7.904 ± 0.213

2.341 ± 0.17

4.802 ± 0.159

4.098 ± 0.178

7.616 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.099

3.611 ± 0.143

4.86 ± 0.17

3.666 ± 0.266

6.95 ± 0.205

5.697 ± 0.139

5.89 ± 0.185

7.208 ± 0.226

2.162 ± 0.108

2.897 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski