Brachyspira catarrhinii

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Brachyspirales; Brachyspiraceae; Brachyspira

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U7N9U6|A0A4U7N9U6_9SPIR ABC transporter substrate-binding protein (Fragment) OS=Brachyspira catarrhinii OX=2528966 GN=EZH24_12920 PE=4 SV=1
MM1 pKa = 7.54ALSISCKK8 pKa = 10.51NEE10 pKa = 3.56DD11 pKa = 3.49KK12 pKa = 11.06TGGGNGGGSGGSIPTASGSPATVGNAEE39 pKa = 4.68FSGTLNNISISGDD52 pKa = 2.74GWTKK56 pKa = 10.01EE57 pKa = 3.96QVDD60 pKa = 4.43ALVMADD66 pKa = 3.97NFSLSIEE73 pKa = 4.2GNNAGCSYY81 pKa = 10.52IVPPKK86 pKa = 10.45QLLCPDD92 pKa = 3.64SSNPNVVEE100 pKa = 4.06TSVEE104 pKa = 4.04EE105 pKa = 4.0VLEE108 pKa = 4.95DD109 pKa = 3.26GTTYY113 pKa = 10.48TIYY116 pKa = 10.43II117 pKa = 4.3

Molecular weight: 11.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U7NBM3|A0A4U7NBM3_9SPIR Uncharacterized protein OS=Brachyspira catarrhinii OX=2528966 GN=EZH24_09655 PE=4 SV=1
MM1 pKa = 7.55TINSISNNTAIRR13 pKa = 11.84LQKK16 pKa = 10.48PNIEE20 pKa = 4.25TQTAKK25 pKa = 10.42PVQNINNNANQNSSLSPLSSRR46 pKa = 11.84YY47 pKa = 7.89TYY49 pKa = 10.86AIGRR53 pKa = 11.84DD54 pKa = 3.34GKK56 pKa = 10.88RR57 pKa = 11.84YY58 pKa = 9.54ILQLRR63 pKa = 11.84IDD65 pKa = 3.8IASVRR70 pKa = 11.84AFSALAA76 pKa = 3.68

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2664

0

2664

749613

24

3264

281.4

32.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.48 ± 0.05

0.955 ± 0.019

5.645 ± 0.045

7.316 ± 0.066

5.076 ± 0.051

5.59 ± 0.056

1.255 ± 0.018

10.708 ± 0.058

9.319 ± 0.067

8.694 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.022

7.735 ± 0.062

2.678 ± 0.029

2.017 ± 0.02

3.376 ± 0.028

6.597 ± 0.041

4.538 ± 0.037

5.141 ± 0.043

0.679 ± 0.014

4.845 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski