Phormidium willei BDU 130791

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Oscillatoriaceae; Phormidium; Phormidium willei

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168UTS5|A0A168UTS5_9CYAN GTPase Obg OS=Phormidium willei BDU 130791 OX=399101 GN=obg PE=3 SV=1
MM1 pKa = 7.65PFWSAQQQNYY11 pKa = 9.03QDD13 pKa = 3.23TGGLVFSPGTSGHH26 pKa = 5.97IQVTPQDD33 pKa = 3.72GSNEE37 pKa = 3.99VLDD40 pKa = 6.19LPFLWWMPYY49 pKa = 7.46QTHH52 pKa = 6.14ATTFYY57 pKa = 8.81WWEE60 pKa = 4.28DD61 pKa = 3.57YY62 pKa = 10.74EE63 pKa = 5.18LSDD66 pKa = 4.69GGSLMTEE73 pKa = 3.89SLSAINFVRR82 pKa = 11.84FTSYY86 pKa = 11.13SDD88 pKa = 3.46PDD90 pKa = 3.22EE91 pKa = 4.89FYY93 pKa = 11.61YY94 pKa = 11.32LLLPNIDD101 pKa = 4.28PDD103 pKa = 3.97PFLSFDD109 pKa = 3.97SIYY112 pKa = 10.99LEE114 pKa = 4.39SEE116 pKa = 3.85NANPTNDD123 pKa = 3.32IPLLTSLINSEE134 pKa = 4.43YY135 pKa = 9.8EE136 pKa = 4.08VNRR139 pKa = 11.84ITASNVGFEE148 pKa = 5.44DD149 pKa = 2.95ILTFEE154 pKa = 5.03RR155 pKa = 11.84PDD157 pKa = 3.54LLQPEE162 pKa = 4.81PEE164 pKa = 4.39VPLNPPSPEE173 pKa = 4.18SPDD176 pKa = 3.49SQDD179 pKa = 3.31VPEE182 pKa = 4.4VTSPLSLILIGIILAGSILKK202 pKa = 10.29RR203 pKa = 11.84RR204 pKa = 11.84FTCRR208 pKa = 11.84MATQHH213 pKa = 6.29RR214 pKa = 4.14

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166SD16|A0A166SD16_9CYAN Hybrid sensor histidine kinase/response regulator OS=Phormidium willei BDU 130791 OX=399101 GN=AY600_09930 PE=4 SV=1
MM1 pKa = 7.41TKK3 pKa = 8.97RR4 pKa = 11.84TFGGTVRR11 pKa = 11.84KK12 pKa = 9.62RR13 pKa = 11.84KK14 pKa = 8.12RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84IKK26 pKa = 9.64TGRR29 pKa = 11.84RR30 pKa = 11.84VINSRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.83GRR40 pKa = 11.84ARR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1257126

29

5310

333.5

37.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.992 ± 0.058

1.002 ± 0.013

5.673 ± 0.043

6.49 ± 0.048

3.645 ± 0.027

7.258 ± 0.043

2.03 ± 0.022

5.426 ± 0.035

3.089 ± 0.037

11.543 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.865 ± 0.018

3.302 ± 0.029

5.299 ± 0.036

5.429 ± 0.039

6.552 ± 0.034

6.149 ± 0.034

5.27 ± 0.024

6.696 ± 0.035

1.545 ± 0.018

2.746 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski