Beihai barnacle virus 8

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Artoviridae; Hexartovirus; Barnacle hexartovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMV2|A0A1L3KMV2_9MONO GDP polyribonucleotidyltransferase OS=Beihai barnacle virus 8 OX=1922366 PE=4 SV=1
MM1 pKa = 7.58ANADD5 pKa = 3.88PGDD8 pKa = 3.59EE9 pKa = 3.91RR10 pKa = 11.84LRR12 pKa = 11.84ALEE15 pKa = 4.06TFSSILHH22 pKa = 5.96HH23 pKa = 6.68LGIEE27 pKa = 4.12AEE29 pKa = 4.03EE30 pKa = 4.34SNRR33 pKa = 11.84GFVQLGTVQHH43 pKa = 5.72VSYY46 pKa = 11.01LFGKK50 pKa = 7.28QTEE53 pKa = 4.15PAIVHH58 pKa = 5.14TTSDD62 pKa = 3.83LLRR65 pKa = 11.84QTNEE69 pKa = 3.66FAPDD73 pKa = 2.98EE74 pKa = 4.43GFFLSGMLTRR84 pKa = 11.84GGKK87 pKa = 9.73SYY89 pKa = 10.76TSLYY93 pKa = 9.83VIQDD97 pKa = 3.54KK98 pKa = 11.09VLEE101 pKa = 4.4DD102 pKa = 3.52QGLLLNIQRR111 pKa = 11.84RR112 pKa = 11.84KK113 pKa = 10.04EE114 pKa = 3.89LLDD117 pKa = 3.48MSEE120 pKa = 4.09MTLKK124 pKa = 10.59RR125 pKa = 11.84MKK127 pKa = 10.49QLGKK131 pKa = 8.95SAKK134 pKa = 9.93VVFQSDD140 pKa = 4.5VINSIPIHH148 pKa = 5.29LVKK151 pKa = 10.65PEE153 pKa = 4.46LLPEE157 pKa = 4.2IAADD161 pKa = 3.63NRR163 pKa = 11.84LSRR166 pKa = 11.84ASKK169 pKa = 9.55VASALFSVFPWTLDD183 pKa = 3.12NASAVFHH190 pKa = 6.0AQQYY194 pKa = 10.21DD195 pKa = 3.61KK196 pKa = 10.9EE197 pKa = 4.27ASALEE202 pKa = 4.22LPPGVNAQSEE212 pKa = 4.3EE213 pKa = 4.12GAAQLDD219 pKa = 3.92TALRR223 pKa = 11.84NQFHH227 pKa = 6.01QMVIAVGTLCLCYY240 pKa = 10.2GYY242 pKa = 10.6SQDD245 pKa = 4.9NLIAKK250 pKa = 8.9QISRR254 pKa = 11.84RR255 pKa = 11.84LAAAYY260 pKa = 10.43ADD262 pKa = 4.36ANLAAPDD269 pKa = 3.79WDD271 pKa = 3.73TVANNIKK278 pKa = 9.46EE279 pKa = 4.34WAGSPRR285 pKa = 11.84EE286 pKa = 3.78IHH288 pKa = 6.34GEE290 pKa = 4.08VVALIGRR297 pKa = 11.84VLALHH302 pKa = 7.19RR303 pKa = 11.84DD304 pKa = 3.63GSINPSLAAEE314 pKa = 4.5DD315 pKa = 4.07EE316 pKa = 4.39ALSAEE321 pKa = 4.32DD322 pKa = 3.46PRR324 pKa = 11.84LRR326 pKa = 11.84NLPPIFNAVRR336 pKa = 11.84DD337 pKa = 3.95QMLLVYY343 pKa = 9.87TNYY346 pKa = 9.95QSSTIRR352 pKa = 11.84WALQVLPLVEE362 pKa = 3.92EE363 pKa = 4.58AGFGTHH369 pKa = 6.09HH370 pKa = 7.29RR371 pKa = 11.84LGAEE375 pKa = 3.49IRR377 pKa = 11.84HH378 pKa = 5.83LKK380 pKa = 9.38SIKK383 pKa = 10.72GEE385 pKa = 3.89IEE387 pKa = 3.51GRR389 pKa = 11.84KK390 pKa = 9.37YY391 pKa = 10.79VGLVSKK397 pKa = 10.56IATRR401 pKa = 11.84HH402 pKa = 4.29QQQQFPMMSFIGCMYY417 pKa = 10.56HH418 pKa = 7.8SMHH421 pKa = 7.32LEE423 pKa = 4.08TEE425 pKa = 4.16DD426 pKa = 2.94QKK428 pKa = 11.97AAFRR432 pKa = 11.84DD433 pKa = 4.34FNLTGISQKK442 pKa = 10.53VADD445 pKa = 4.18VQSKK449 pKa = 8.24PVCDD453 pKa = 4.33AVLKK457 pKa = 8.53MLPAPHH463 pKa = 6.83IEE465 pKa = 4.18AKK467 pKa = 10.31ASLMKK472 pKa = 10.64GMSALDD478 pKa = 3.57VEE480 pKa = 5.13RR481 pKa = 11.84VLSASPEE488 pKa = 4.17DD489 pKa = 3.82EE490 pKa = 4.12VKK492 pKa = 10.69LLKK495 pKa = 10.37EE496 pKa = 4.04RR497 pKa = 11.84LRR499 pKa = 11.84GVDD502 pKa = 4.81PPCDD506 pKa = 3.48WAIHH510 pKa = 5.52DD511 pKa = 4.64AKK513 pKa = 10.65EE514 pKa = 3.67QAAEE518 pKa = 4.06AKK520 pKa = 10.3KK521 pKa = 10.63KK522 pKa = 10.26FYY524 pKa = 10.34QAAALEE530 pKa = 4.58LNTRR534 pKa = 11.84WEE536 pKa = 4.21KK537 pKa = 11.12AHH539 pKa = 5.91ATMMDD544 pKa = 4.93RR545 pKa = 11.84ITMEE549 pKa = 4.22ANAEE553 pKa = 4.09TRR555 pKa = 11.84QRR557 pKa = 11.84LAQGIQTLRR566 pKa = 11.84AEE568 pKa = 4.64LLRR571 pKa = 11.84LTTAEE576 pKa = 4.13EE577 pKa = 4.3MSGSDD582 pKa = 5.26LMKK585 pKa = 10.37QLQITYY591 pKa = 9.97RR592 pKa = 11.84ADD594 pKa = 4.4LEE596 pKa = 4.46EE597 pKa = 4.08HH598 pKa = 6.38WKK600 pKa = 11.05AGMAVIQKK608 pKa = 9.92LRR610 pKa = 11.84EE611 pKa = 3.94LL612 pKa = 4.02

Molecular weight: 68.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMP7|A0A1L3KMP7_9MONO Putative glycoprotein OS=Beihai barnacle virus 8 OX=1922366 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 6.51DD3 pKa = 4.21LPEE6 pKa = 4.34HH7 pKa = 6.34LKK9 pKa = 10.15EE10 pKa = 4.25LKK12 pKa = 10.55VLADD16 pKa = 3.3HH17 pKa = 6.32KK18 pKa = 9.64TYY20 pKa = 10.73LKK22 pKa = 10.39KK23 pKa = 10.42PPSTGEE29 pKa = 4.18GTSSRR34 pKa = 11.84PEE36 pKa = 3.79SSPEE40 pKa = 3.71EE41 pKa = 3.87MFSASEE47 pKa = 4.13LSPSGQATPPASLRR61 pKa = 11.84TPLSGSQEE69 pKa = 4.25TLKK72 pKa = 10.88SHH74 pKa = 5.99IPVRR78 pKa = 11.84AGTTPPSSGQSLPPRR93 pKa = 11.84KK94 pKa = 9.62LSLHH98 pKa = 5.42SAEE101 pKa = 4.56CQTVEE106 pKa = 4.4PAHH109 pKa = 6.17SAASALLAWSGNYY122 pKa = 9.12KK123 pKa = 10.57KK124 pKa = 10.03PDD126 pKa = 3.12LVAAYY131 pKa = 7.36EE132 pKa = 4.04AWALNSTAPWKK143 pKa = 10.39KK144 pKa = 9.71ISGMYY149 pKa = 10.21KK150 pKa = 9.91KK151 pKa = 10.38FFEE154 pKa = 4.75AFPSYY159 pKa = 10.41SIKK162 pKa = 10.64EE163 pKa = 4.13PLSLKK168 pKa = 9.77PPKK171 pKa = 10.66GSDD174 pKa = 3.13FSQEE178 pKa = 3.39VWNTILSFIWEE189 pKa = 4.39DD190 pKa = 3.69CLDD193 pKa = 3.85NLIFCPRR200 pKa = 11.84EE201 pKa = 4.12MKK203 pKa = 10.4LHH205 pKa = 6.83WYY207 pKa = 10.18ACIAPPAEE215 pKa = 4.39CNKK218 pKa = 10.73SKK220 pKa = 10.68DD221 pKa = 3.46AHH223 pKa = 6.14CRR225 pKa = 11.84LAARR229 pKa = 11.84HH230 pKa = 5.02LHH232 pKa = 4.53VLKK235 pKa = 10.54MSLYY239 pKa = 9.75PSDD242 pKa = 3.72ATEE245 pKa = 3.61SDD247 pKa = 3.84YY248 pKa = 11.66LDD250 pKa = 4.21RR251 pKa = 11.84SPMTQLGEE259 pKa = 4.34SVMLLSSAGGLCRR272 pKa = 11.84AAKK275 pKa = 9.84AVSEE279 pKa = 4.42STHH282 pKa = 5.24QQVAMCKK289 pKa = 10.11DD290 pKa = 3.15AAAHH294 pKa = 4.81QKK296 pKa = 10.4IMLDD300 pKa = 3.2AATDD304 pKa = 3.47KK305 pKa = 9.1MTEE308 pKa = 3.73TSLYY312 pKa = 9.61CSQIMDD318 pKa = 4.37HH319 pKa = 6.81KK320 pKa = 10.16AQHH323 pKa = 6.44LSRR326 pKa = 11.84YY327 pKa = 9.88LDD329 pKa = 3.38LTMKK333 pKa = 10.55KK334 pKa = 10.52DD335 pKa = 3.9DD336 pKa = 3.63VDD338 pKa = 3.49TGKK341 pKa = 10.78FMAGSASVVTAPSEE355 pKa = 4.22RR356 pKa = 11.84LSSTSRR362 pKa = 11.84LSRR365 pKa = 11.84TSSAKK370 pKa = 10.39SSVSKK375 pKa = 9.87ATSAKK380 pKa = 9.77RR381 pKa = 11.84AQTGRR386 pKa = 11.84ATFPQVQKK394 pKa = 11.19LSS396 pKa = 3.58

Molecular weight: 43.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3854

396

2268

963.5

106.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.626 ± 0.564

1.505 ± 0.27

4.826 ± 0.269

6.046 ± 0.387

2.984 ± 0.181

5.864 ± 0.464

3.14 ± 0.15

4.307 ± 0.543

4.281 ± 0.846

11.417 ± 0.791

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.319

2.309 ± 0.271

5.942 ± 0.566

4.463 ± 0.479

6.668 ± 0.701

8.329 ± 1.349

5.864 ± 0.67

5.838 ± 0.49

1.842 ± 0.198

2.387 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski