Acinetobacter phage Henu6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Twarogvirinae; Zedzedvirus; unclassified Zedzedvirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A410T5J9|A0A410T5J9_9CAUD Uncharacterized protein OS=Acinetobacter phage Henu6 OX=2500136 GN=Henu6_gp205 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84HH3 pKa = 5.09MMEE6 pKa = 4.85NLIQPTEE13 pKa = 3.95EE14 pKa = 3.74QFEE17 pKa = 4.42QIRR20 pKa = 11.84DD21 pKa = 3.81EE22 pKa = 5.7LEE24 pKa = 4.19GCSDD28 pKa = 4.16DD29 pKa = 4.49FGRR32 pKa = 11.84THH34 pKa = 6.82KK35 pKa = 10.59RR36 pKa = 11.84VLTEE40 pKa = 4.72LGLDD44 pKa = 3.25TDD46 pKa = 4.54LRR48 pKa = 11.84DD49 pKa = 3.59SVIYY53 pKa = 10.8NDD55 pKa = 3.66TLEE58 pKa = 4.63TYY60 pKa = 9.95TFLCEE65 pKa = 6.07NCDD68 pKa = 3.79CWCSPDD74 pKa = 3.58TRR76 pKa = 11.84VHH78 pKa = 5.2NQIAEE83 pKa = 4.09MTVCEE88 pKa = 4.34EE89 pKa = 4.57CDD91 pKa = 3.44EE92 pKa = 4.19QLSS95 pKa = 3.76

Molecular weight:
11.18 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A410T5N8|A0A410T5N8_9CAUD Uncharacterized protein OS=Acinetobacter phage Henu6 OX=2500136 GN=Henu6_gp25 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84VLQNQLKK9 pKa = 8.78EE10 pKa = 4.07VSFFTSCINIKK21 pKa = 9.03KK22 pKa = 7.78TFNVFLNTVQFIIIGTVSNTYY43 pKa = 10.29RR44 pKa = 11.84CNTWPRR50 pKa = 11.84PHH52 pKa = 6.72GFHH55 pKa = 5.72YY56 pKa = 9.65TFNISASRR64 pKa = 11.84NNKK67 pKa = 9.39LCLL70 pKa = 3.51

Molecular weight:
8.22 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

234

0

234

51348

68

1385

219.4

24.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.023 ± 0.205

1.071 ± 0.064

6.168 ± 0.126

6.429 ± 0.211

4.281 ± 0.123

5.809 ± 0.238

1.914 ± 0.088

7.375 ± 0.143

7.383 ± 0.189

8.02 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.096

5.93 ± 0.133

3.628 ± 0.097

3.554 ± 0.113

4.172 ± 0.107

6.251 ± 0.144

6.364 ± 0.231

6.477 ± 0.123

1.34 ± 0.055

4.101 ± 0.127

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski