Amino acid dipepetide frequency for Lactococcus phage CHPC1175

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.598AlaAla: 5.598 ± 2.084
0.355AlaCys: 0.355 ± 0.184
4.976AlaAsp: 4.976 ± 0.816
4.798AlaGlu: 4.798 ± 0.952
2.31AlaPhe: 2.31 ± 0.59
4.62AlaGly: 4.62 ± 0.75
0.977AlaHis: 0.977 ± 0.315
4.976AlaIle: 4.976 ± 0.71
5.42AlaLys: 5.42 ± 0.972
4.709AlaLeu: 4.709 ± 0.903
2.044AlaMet: 2.044 ± 0.426
3.909AlaAsn: 3.909 ± 0.57
1.688AlaPro: 1.688 ± 0.44
4.354AlaGln: 4.354 ± 1.358
2.044AlaArg: 2.044 ± 0.518
4.62AlaSer: 4.62 ± 0.963
4.176AlaThr: 4.176 ± 1.006
3.821AlaVal: 3.821 ± 0.594
1.066AlaTrp: 1.066 ± 0.383
3.199AlaTyr: 3.199 ± 0.536
0.0AlaXaa: 0.0 ± 0.0
Cys
0.178CysAla: 0.178 ± 0.121
0.178CysCys: 0.178 ± 0.129
0.267CysAsp: 0.267 ± 0.193
0.355CysGlu: 0.355 ± 0.202
0.089CysPhe: 0.089 ± 0.091
0.444CysGly: 0.444 ± 0.257
0.444CysHis: 0.444 ± 0.332
0.178CysIle: 0.178 ± 0.138
0.533CysLys: 0.533 ± 0.236
0.267CysLeu: 0.267 ± 0.142
0.089CysMet: 0.089 ± 0.092
0.267CysAsn: 0.267 ± 0.166
0.178CysPro: 0.178 ± 0.138
0.178CysGln: 0.178 ± 0.132
0.178CysArg: 0.178 ± 0.143
0.0CysSer: 0.0 ± 0.0
0.355CysThr: 0.355 ± 0.21
0.355CysVal: 0.355 ± 0.173
0.533CysTrp: 0.533 ± 0.254
0.178CysTyr: 0.178 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
3.376AspAla: 3.376 ± 0.724
0.355AspCys: 0.355 ± 0.232
4.176AspAsp: 4.176 ± 0.705
4.62AspGlu: 4.62 ± 0.817
3.821AspPhe: 3.821 ± 0.523
6.219AspGly: 6.219 ± 1.99
0.622AspHis: 0.622 ± 0.218
4.531AspIle: 4.531 ± 0.617
5.686AspLys: 5.686 ± 0.806
4.442AspLeu: 4.442 ± 0.741
1.599AspMet: 1.599 ± 0.391
3.199AspAsn: 3.199 ± 0.543
2.221AspPro: 2.221 ± 0.499
2.044AspGln: 2.044 ± 0.462
2.399AspArg: 2.399 ± 0.516
3.998AspSer: 3.998 ± 0.498
2.665AspThr: 2.665 ± 0.482
4.176AspVal: 4.176 ± 0.674
1.333AspTrp: 1.333 ± 0.359
2.399AspTyr: 2.399 ± 0.467
0.089AspXaa: 0.089 ± 0.094
Glu
4.531GluAla: 4.531 ± 0.877
0.533GluCys: 0.533 ± 0.295
2.665GluAsp: 2.665 ± 0.639
4.265GluGlu: 4.265 ± 0.626
2.31GluPhe: 2.31 ± 0.34
2.577GluGly: 2.577 ± 0.458
1.333GluHis: 1.333 ± 0.371
4.976GluIle: 4.976 ± 0.648
6.308GluLys: 6.308 ± 0.986
5.509GluLeu: 5.509 ± 0.855
1.688GluMet: 1.688 ± 0.421
4.087GluAsn: 4.087 ± 0.741
1.422GluPro: 1.422 ± 0.42
3.643GluGln: 3.643 ± 0.568
1.777GluArg: 1.777 ± 0.477
3.287GluSer: 3.287 ± 0.55
2.932GluThr: 2.932 ± 0.667
4.531GluVal: 4.531 ± 0.744
2.044GluTrp: 2.044 ± 0.501
2.577GluTyr: 2.577 ± 0.403
0.0GluXaa: 0.0 ± 0.0
Phe
2.399PheAla: 2.399 ± 0.452
0.533PheCys: 0.533 ± 0.215
3.199PheAsp: 3.199 ± 0.635
2.132PheGlu: 2.132 ± 0.562
1.599PhePhe: 1.599 ± 0.301
2.31PheGly: 2.31 ± 0.468
0.444PheHis: 0.444 ± 0.211
2.843PheIle: 2.843 ± 0.491
2.577PheLys: 2.577 ± 0.482
3.465PheLeu: 3.465 ± 0.633
1.422PheMet: 1.422 ± 0.455
2.399PheAsn: 2.399 ± 0.378
1.155PhePro: 1.155 ± 0.338
1.155PheGln: 1.155 ± 0.325
1.955PheArg: 1.955 ± 0.378
2.843PheSer: 2.843 ± 0.581
2.132PheThr: 2.132 ± 0.473
2.577PheVal: 2.577 ± 0.455
0.444PheTrp: 0.444 ± 0.225
1.777PheTyr: 1.777 ± 0.411
0.0PheXaa: 0.0 ± 0.0
Gly
3.021GlyAla: 3.021 ± 0.74
0.089GlyCys: 0.089 ± 0.092
3.821GlyAsp: 3.821 ± 0.991
2.932GlyGlu: 2.932 ± 0.58
3.998GlyPhe: 3.998 ± 0.819
4.62GlyGly: 4.62 ± 1.045
0.888GlyHis: 0.888 ± 0.371
5.153GlyIle: 5.153 ± 0.818
5.686GlyLys: 5.686 ± 0.625
4.442GlyLeu: 4.442 ± 0.569
2.132GlyMet: 2.132 ± 0.35
4.176GlyAsn: 4.176 ± 0.579
0.977GlyPro: 0.977 ± 0.275
3.199GlyGln: 3.199 ± 0.603
2.31GlyArg: 2.31 ± 0.535
4.354GlySer: 4.354 ± 0.834
5.686GlyThr: 5.686 ± 1.351
3.732GlyVal: 3.732 ± 0.66
1.155GlyTrp: 1.155 ± 0.301
3.199GlyTyr: 3.199 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
1.066HisAla: 1.066 ± 0.339
0.0HisCys: 0.0 ± 0.0
1.066HisAsp: 1.066 ± 0.352
1.422HisGlu: 1.422 ± 0.492
0.444HisPhe: 0.444 ± 0.245
0.622HisGly: 0.622 ± 0.261
0.089HisHis: 0.089 ± 0.087
0.888HisIle: 0.888 ± 0.314
1.155HisLys: 1.155 ± 0.365
0.977HisLeu: 0.977 ± 0.349
0.444HisMet: 0.444 ± 0.189
1.244HisAsn: 1.244 ± 0.301
0.622HisPro: 0.622 ± 0.211
0.711HisGln: 0.711 ± 0.31
0.178HisArg: 0.178 ± 0.15
1.066HisSer: 1.066 ± 0.345
0.355HisThr: 0.355 ± 0.129
0.267HisVal: 0.267 ± 0.191
0.0HisTrp: 0.0 ± 0.0
0.355HisTyr: 0.355 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.42IleAla: 5.42 ± 0.775
0.089IleCys: 0.089 ± 0.094
4.887IleAsp: 4.887 ± 0.702
3.287IleGlu: 3.287 ± 0.596
1.777IlePhe: 1.777 ± 0.47
4.887IleGly: 4.887 ± 0.75
0.533IleHis: 0.533 ± 0.211
4.709IleIle: 4.709 ± 0.768
5.775IleLys: 5.775 ± 0.719
4.087IleLeu: 4.087 ± 0.491
1.777IleMet: 1.777 ± 0.368
6.308IleAsn: 6.308 ± 0.859
1.955IlePro: 1.955 ± 0.351
2.577IleGln: 2.577 ± 0.63
3.554IleArg: 3.554 ± 0.514
5.331IleSer: 5.331 ± 0.833
3.643IleThr: 3.643 ± 0.709
3.821IleVal: 3.821 ± 0.502
0.533IleTrp: 0.533 ± 0.168
2.399IleTyr: 2.399 ± 0.561
0.0IleXaa: 0.0 ± 0.0
Lys
6.397LysAla: 6.397 ± 1.846
0.533LysCys: 0.533 ± 0.3
5.509LysAsp: 5.509 ± 1.087
5.242LysGlu: 5.242 ± 1.102
3.376LysPhe: 3.376 ± 0.6
5.42LysGly: 5.42 ± 1.063
1.51LysHis: 1.51 ± 0.486
5.509LysIle: 5.509 ± 0.837
7.73LysLys: 7.73 ± 1.476
7.019LysLeu: 7.019 ± 1.066
2.31LysMet: 2.31 ± 0.601
6.841LysAsn: 6.841 ± 1.023
2.399LysPro: 2.399 ± 0.593
4.531LysGln: 4.531 ± 0.824
3.021LysArg: 3.021 ± 0.583
4.976LysSer: 4.976 ± 0.657
6.308LysThr: 6.308 ± 0.75
4.62LysVal: 4.62 ± 0.569
0.888LysTrp: 0.888 ± 0.268
2.577LysTyr: 2.577 ± 0.411
0.0LysXaa: 0.0 ± 0.0
Leu
5.064LeuAla: 5.064 ± 0.584
0.533LeuCys: 0.533 ± 0.222
5.42LeuAsp: 5.42 ± 0.885
4.62LeuGlu: 4.62 ± 0.854
1.688LeuPhe: 1.688 ± 0.437
3.909LeuGly: 3.909 ± 0.662
0.977LeuHis: 0.977 ± 0.272
5.331LeuIle: 5.331 ± 0.751
6.753LeuLys: 6.753 ± 0.843
5.598LeuLeu: 5.598 ± 1.086
2.132LeuMet: 2.132 ± 0.569
6.308LeuAsn: 6.308 ± 0.878
2.665LeuPro: 2.665 ± 0.55
3.643LeuGln: 3.643 ± 0.633
2.132LeuArg: 2.132 ± 0.539
7.819LeuSer: 7.819 ± 0.762
4.62LeuThr: 4.62 ± 0.841
4.087LeuVal: 4.087 ± 0.73
0.8LeuTrp: 0.8 ± 0.286
1.777LeuTyr: 1.777 ± 0.491
0.0LeuXaa: 0.0 ± 0.0
Met
2.665MetAla: 2.665 ± 0.531
0.0MetCys: 0.0 ± 0.0
1.333MetAsp: 1.333 ± 0.389
1.777MetGlu: 1.777 ± 0.485
0.888MetPhe: 0.888 ± 0.35
1.422MetGly: 1.422 ± 0.325
0.089MetHis: 0.089 ± 0.101
1.066MetIle: 1.066 ± 0.372
3.287MetLys: 3.287 ± 0.659
1.599MetLeu: 1.599 ± 0.468
0.977MetMet: 0.977 ± 0.32
1.777MetAsn: 1.777 ± 0.527
0.444MetPro: 0.444 ± 0.212
1.155MetGln: 1.155 ± 0.364
0.888MetArg: 0.888 ± 0.287
2.932MetSer: 2.932 ± 0.555
2.932MetThr: 2.932 ± 0.562
1.244MetVal: 1.244 ± 0.375
0.267MetTrp: 0.267 ± 0.137
0.622MetTyr: 0.622 ± 0.238
0.0MetXaa: 0.0 ± 0.0
Asn
4.087AsnAla: 4.087 ± 0.624
0.267AsnCys: 0.267 ± 0.164
4.087AsnAsp: 4.087 ± 0.667
3.909AsnGlu: 3.909 ± 0.497
2.399AsnPhe: 2.399 ± 0.579
5.331AsnGly: 5.331 ± 0.892
0.711AsnHis: 0.711 ± 0.292
3.732AsnIle: 3.732 ± 0.777
5.242AsnLys: 5.242 ± 0.84
5.509AsnLeu: 5.509 ± 0.724
1.866AsnMet: 1.866 ± 0.521
4.087AsnAsn: 4.087 ± 0.639
2.665AsnPro: 2.665 ± 0.7
3.376AsnGln: 3.376 ± 0.948
3.021AsnArg: 3.021 ± 0.601
6.308AsnSer: 6.308 ± 0.903
3.732AsnThr: 3.732 ± 0.555
3.199AsnVal: 3.199 ± 0.529
0.444AsnTrp: 0.444 ± 0.242
2.044AsnTyr: 2.044 ± 0.536
0.0AsnXaa: 0.0 ± 0.0
Pro
2.044ProAla: 2.044 ± 0.507
0.089ProCys: 0.089 ± 0.094
1.422ProAsp: 1.422 ± 0.368
1.599ProGlu: 1.599 ± 0.409
1.422ProPhe: 1.422 ± 0.447
1.422ProGly: 1.422 ± 0.574
0.267ProHis: 0.267 ± 0.157
1.599ProIle: 1.599 ± 0.404
1.866ProLys: 1.866 ± 0.445
1.866ProLeu: 1.866 ± 0.425
0.711ProMet: 0.711 ± 0.27
1.688ProAsn: 1.688 ± 0.575
0.711ProPro: 0.711 ± 0.285
1.51ProGln: 1.51 ± 0.361
0.888ProArg: 0.888 ± 0.314
2.665ProSer: 2.665 ± 0.633
2.932ProThr: 2.932 ± 0.767
1.51ProVal: 1.51 ± 0.426
0.444ProTrp: 0.444 ± 0.252
1.066ProTyr: 1.066 ± 0.319
0.0ProXaa: 0.0 ± 0.0
Gln
4.354GlnAla: 4.354 ± 0.628
0.089GlnCys: 0.089 ± 0.091
2.31GlnAsp: 2.31 ± 0.484
2.577GlnGlu: 2.577 ± 0.52
1.866GlnPhe: 1.866 ± 0.409
3.376GlnGly: 3.376 ± 0.669
0.178GlnHis: 0.178 ± 0.106
3.021GlnIle: 3.021 ± 0.566
4.798GlnLys: 4.798 ± 1.428
4.798GlnLeu: 4.798 ± 0.819
1.244GlnMet: 1.244 ± 0.47
2.044GlnAsn: 2.044 ± 0.539
0.711GlnPro: 0.711 ± 0.276
2.31GlnGln: 2.31 ± 0.755
1.155GlnArg: 1.155 ± 0.409
3.643GlnSer: 3.643 ± 0.565
2.843GlnThr: 2.843 ± 0.575
2.399GlnVal: 2.399 ± 0.517
0.8GlnTrp: 0.8 ± 0.329
2.044GlnTyr: 2.044 ± 0.414
0.0GlnXaa: 0.0 ± 0.0
Arg
1.51ArgAla: 1.51 ± 0.422
0.355ArgCys: 0.355 ± 0.18
2.132ArgAsp: 2.132 ± 0.465
3.287ArgGlu: 3.287 ± 0.903
1.777ArgPhe: 1.777 ± 0.41
2.754ArgGly: 2.754 ± 0.783
0.267ArgHis: 0.267 ± 0.176
1.599ArgIle: 1.599 ± 0.353
3.021ArgLys: 3.021 ± 0.479
2.843ArgLeu: 2.843 ± 0.52
0.888ArgMet: 0.888 ± 0.302
3.021ArgAsn: 3.021 ± 0.639
1.066ArgPro: 1.066 ± 0.364
1.51ArgGln: 1.51 ± 0.448
0.888ArgArg: 0.888 ± 0.312
2.044ArgSer: 2.044 ± 0.462
1.777ArgThr: 1.777 ± 0.555
2.044ArgVal: 2.044 ± 0.509
0.8ArgTrp: 0.8 ± 0.306
1.066ArgTyr: 1.066 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
4.176SerAla: 4.176 ± 0.787
0.178SerCys: 0.178 ± 0.133
5.864SerAsp: 5.864 ± 0.856
5.064SerGlu: 5.064 ± 0.631
3.11SerPhe: 3.11 ± 0.523
5.242SerGly: 5.242 ± 0.732
1.333SerHis: 1.333 ± 0.327
3.199SerIle: 3.199 ± 0.444
5.864SerLys: 5.864 ± 0.794
5.953SerLeu: 5.953 ± 0.726
2.132SerMet: 2.132 ± 0.486
5.242SerAsn: 5.242 ± 0.709
1.688SerPro: 1.688 ± 0.5
3.554SerGln: 3.554 ± 0.553
2.399SerArg: 2.399 ± 0.433
5.775SerSer: 5.775 ± 0.868
4.709SerThr: 4.709 ± 0.976
4.798SerVal: 4.798 ± 0.462
1.333SerTrp: 1.333 ± 0.289
3.11SerTyr: 3.11 ± 0.6
0.0SerXaa: 0.0 ± 0.0
Thr
5.064ThrAla: 5.064 ± 0.92
0.355ThrCys: 0.355 ± 0.257
3.909ThrAsp: 3.909 ± 0.505
3.909ThrGlu: 3.909 ± 0.756
2.044ThrPhe: 2.044 ± 0.62
4.798ThrGly: 4.798 ± 0.578
0.711ThrHis: 0.711 ± 0.183
5.331ThrIle: 5.331 ± 0.77
4.798ThrLys: 4.798 ± 0.825
3.909ThrLeu: 3.909 ± 0.989
1.599ThrMet: 1.599 ± 0.455
4.354ThrAsn: 4.354 ± 0.949
1.777ThrPro: 1.777 ± 0.692
2.221ThrGln: 2.221 ± 0.642
2.399ThrArg: 2.399 ± 0.425
3.732ThrSer: 3.732 ± 0.762
5.064ThrThr: 5.064 ± 1.326
4.976ThrVal: 4.976 ± 1.075
0.977ThrTrp: 0.977 ± 0.294
2.132ThrTyr: 2.132 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
5.509ValAla: 5.509 ± 0.729
0.089ValCys: 0.089 ± 0.101
4.176ValAsp: 4.176 ± 0.636
3.909ValGlu: 3.909 ± 0.615
2.31ValPhe: 2.31 ± 0.641
2.577ValGly: 2.577 ± 0.455
0.444ValHis: 0.444 ± 0.208
4.62ValIle: 4.62 ± 0.703
5.153ValLys: 5.153 ± 0.913
4.709ValLeu: 4.709 ± 0.636
1.066ValMet: 1.066 ± 0.493
3.376ValAsn: 3.376 ± 0.667
2.132ValPro: 2.132 ± 0.498
2.044ValGln: 2.044 ± 0.505
1.688ValArg: 1.688 ± 0.606
4.62ValSer: 4.62 ± 0.853
3.821ValThr: 3.821 ± 0.67
3.643ValVal: 3.643 ± 0.644
0.533ValTrp: 0.533 ± 0.265
1.422ValTyr: 1.422 ± 0.438
0.0ValXaa: 0.0 ± 0.0
Trp
0.888TrpAla: 0.888 ± 0.257
0.178TrpCys: 0.178 ± 0.135
0.444TrpAsp: 0.444 ± 0.264
0.622TrpGlu: 0.622 ± 0.283
0.444TrpPhe: 0.444 ± 0.164
0.8TrpGly: 0.8 ± 0.242
0.444TrpHis: 0.444 ± 0.236
1.244TrpIle: 1.244 ± 0.398
1.688TrpLys: 1.688 ± 0.468
1.333TrpLeu: 1.333 ± 0.357
0.178TrpMet: 0.178 ± 0.133
0.622TrpAsn: 0.622 ± 0.276
0.0TrpPro: 0.0 ± 0.0
0.711TrpGln: 0.711 ± 0.288
1.066TrpArg: 1.066 ± 0.332
1.51TrpSer: 1.51 ± 0.538
1.155TrpThr: 1.155 ± 0.432
0.977TrpVal: 0.977 ± 0.374
0.178TrpTrp: 0.178 ± 0.149
0.622TrpTyr: 0.622 ± 0.216
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.31TyrAla: 2.31 ± 0.421
0.444TyrCys: 0.444 ± 0.334
2.399TyrAsp: 2.399 ± 0.365
2.665TyrGlu: 2.665 ± 0.594
1.688TyrPhe: 1.688 ± 0.438
2.132TyrGly: 2.132 ± 0.355
0.711TyrHis: 0.711 ± 0.273
2.843TyrIle: 2.843 ± 0.713
3.376TyrLys: 3.376 ± 0.492
2.843TyrLeu: 2.843 ± 0.714
0.977TyrMet: 0.977 ± 0.277
1.066TyrAsn: 1.066 ± 0.306
1.244TyrPro: 1.244 ± 0.397
2.132TyrGln: 2.132 ± 0.585
0.711TyrArg: 0.711 ± 0.273
3.199TyrSer: 3.199 ± 0.521
2.221TyrThr: 2.221 ± 0.656
1.066TyrVal: 1.066 ± 0.272
0.533TyrTrp: 0.533 ± 0.26
1.599TyrTyr: 1.599 ± 0.427
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.089XaaGly: 0.089 ± 0.094
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (11256 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski