Cyanophage KBS-S-1A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; unclassified Autographiviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8EXJ2|G8EXJ2_9CAUD Tail tubular protein (Fragment) OS=Cyanophage KBS-S-1A OX=889952 GN=CPVG_00022 PE=4 SV=1
MM1 pKa = 7.51AVTRR5 pKa = 11.84QDD7 pKa = 3.39YY8 pKa = 8.25TQAGSTITYY17 pKa = 7.29VVPFEE22 pKa = 4.53VIEE25 pKa = 4.2ATDD28 pKa = 2.93IDD30 pKa = 4.55VYY32 pKa = 11.23VNNVLQLQQNTTSTADD48 pKa = 3.12ATHH51 pKa = 6.44PQVISGEE58 pKa = 4.27ITQGTALTNYY68 pKa = 8.32TVASNNGTITFNAVLTAGDD87 pKa = 4.13YY88 pKa = 10.74IVVEE92 pKa = 4.41RR93 pKa = 11.84TTDD96 pKa = 3.37DD97 pKa = 3.1AA98 pKa = 6.67

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8EXK9|G8EXK9_9CAUD Uncharacterized protein (Fragment) OS=Cyanophage KBS-S-1A OX=889952 GN=CPVG_00040 PE=4 SV=1
DDD2 pKa = 3.84RR3 pKa = 11.84TLLNYYY9 pKa = 6.05TRR11 pKa = 11.84IEEE14 pKa = 4.38SNPTLISRR22 pKa = 11.84QTIGRR27 pKa = 11.84ITINWITSSRR37 pKa = 11.84TACLQTSKKK46 pKa = 11.51EEE48 pKa = 4.16SDDD51 pKa = 3.13RR52 pKa = 11.84TAITTEEE59 pKa = 3.79HH60 pKa = 6.98LSLSSSSRR68 pKa = 11.84VNKKK72 pKa = 10.21ATDDD76 pKa = 3.44LTDDD80 pKa = 3.49RR81 pKa = 11.84EEE83 pKa = 3.42RR84 pKa = 11.84YYY86 pKa = 10.4NIDDD90 pKa = 2.96KKK92 pKa = 11.23FLINSRR98 pKa = 11.84NHHH101 pKa = 6.19DDD103 pKa = 3.77ITSISRR109 pKa = 11.84SYYY112 pKa = 11.17ILNCPFRR119 pKa = 11.84VDDD122 pKa = 3.29DD123 pKa = 4.65VPIKKK128 pKa = 10.72IITVFRR134 pKa = 11.84VNVNNTRR141 pKa = 11.84LCSKKK146 pKa = 9.49TTRR149 pKa = 11.84SNTNPSRR156 pKa = 11.84TIPNRR161 pKa = 11.84KKK163 pKa = 8.76VIRR166 pKa = 11.84RR167 pKa = 11.84LLKKK171 pKa = 10.3RR172 pKa = 11.84LTYYY176 pKa = 10.49PVTTSRR182 pKa = 11.84GGVRR186 pKa = 11.84DDD188 pKa = 3.76SVKKK192 pKa = 10.36LRR194 pKa = 11.84GCSSSEEE201 pKa = 3.66VSISRR206 pKa = 11.84TRR208 pKa = 11.84CASILLRR215 pKa = 11.84LSDDD219 pKa = 4.17DD220 pKa = 3.83YYY222 pKa = 11.12FNLRR226 pKa = 11.84KKK228 pKa = 9.92NTTVSNAKKK237 pKa = 10.3YYY239 pKa = 8.64TVSRR243 pKa = 11.84TVNGDDD249 pKa = 4.1IAISNTRR256 pKa = 11.84CSNFNNTSLASSTLISRR273 pKa = 11.84NGDDD277 pKa = 3.47LVRR280 pKa = 11.84VSTASSISKKK290 pKa = 9.93DDD292 pKa = 3.34LAF

Molecular weight: 32.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

7122

49

593

192.5

21.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.972 ± 0.56

1.123 ± 0.22

6.529 ± 0.327

6.108 ± 0.537

3.51 ± 0.251

6.669 ± 0.51

1.755 ± 0.282

5.406 ± 0.277

5.673 ± 0.567

7.989 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.349

5.293 ± 0.353

4.044 ± 0.24

3.777 ± 0.235

5.771 ± 0.47

7.329 ± 0.641

6.655 ± 0.461

6.094 ± 0.412

1.292 ± 0.133

3.58 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski