Halobellus clavatus

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halobellus

Average proteome isoelectric point is 4.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3679 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3DKV8|A0A1H3DKV8_9EURY Uncharacterized protein OS=Halobellus clavatus OX=660517 GN=SAMN04487946_101600 PE=4 SV=1
MM1 pKa = 7.17NAEE4 pKa = 4.75DD5 pKa = 5.65FPTPDD10 pKa = 3.83ADD12 pKa = 3.99VEE14 pKa = 4.87SVTPEE19 pKa = 3.78ALKK22 pKa = 10.91DD23 pKa = 4.14RR24 pKa = 11.84IDD26 pKa = 3.66SGEE29 pKa = 4.38DD30 pKa = 3.07VTILDD35 pKa = 3.74TRR37 pKa = 11.84MQSDD41 pKa = 3.99YY42 pKa = 11.27EE43 pKa = 3.99EE44 pKa = 3.91WHH46 pKa = 6.65IEE48 pKa = 4.03GEE50 pKa = 4.47NVTSINVPYY59 pKa = 10.66FEE61 pKa = 5.8FLDD64 pKa = 3.8EE65 pKa = 6.63DD66 pKa = 3.49IDD68 pKa = 5.64ADD70 pKa = 4.0VLDD73 pKa = 5.41SIPADD78 pKa = 3.27EE79 pKa = 5.19HH80 pKa = 5.44VTVLCAKK87 pKa = 9.84GGASEE92 pKa = 4.41YY93 pKa = 10.77VAGTLVEE100 pKa = 3.96RR101 pKa = 11.84GYY103 pKa = 11.19DD104 pKa = 3.49VDD106 pKa = 3.9HH107 pKa = 7.49LEE109 pKa = 5.55DD110 pKa = 5.15GMNGWASIYY119 pKa = 9.98EE120 pKa = 4.11RR121 pKa = 11.84EE122 pKa = 4.26TVSRR126 pKa = 11.84YY127 pKa = 10.32DD128 pKa = 3.39GAGTLYY134 pKa = 10.35QYY136 pKa = 10.4QRR138 pKa = 11.84PSSGCLGYY146 pKa = 11.19LLVSDD151 pKa = 4.94GEE153 pKa = 4.22AAIVDD158 pKa = 4.1PLRR161 pKa = 11.84AFTDD165 pKa = 3.97RR166 pKa = 11.84YY167 pKa = 10.39LADD170 pKa = 4.15AADD173 pKa = 4.22LGADD177 pKa = 4.07LVYY180 pKa = 11.09ALDD183 pKa = 3.73THH185 pKa = 6.06VHH187 pKa = 6.6ADD189 pKa = 3.66HH190 pKa = 7.01ISGVRR195 pKa = 11.84DD196 pKa = 3.35LDD198 pKa = 4.0DD199 pKa = 4.43EE200 pKa = 4.52GVEE203 pKa = 4.4GVIPAASVDD212 pKa = 3.63RR213 pKa = 11.84GVTYY217 pKa = 10.96ADD219 pKa = 3.69EE220 pKa = 4.35LTNAEE225 pKa = 4.68DD226 pKa = 4.22GDD228 pKa = 4.27VFTVGDD234 pKa = 3.99AEE236 pKa = 4.41VEE238 pKa = 4.36TVYY241 pKa = 10.9TPGHH245 pKa = 4.66TTGMTSYY252 pKa = 11.22LLDD255 pKa = 4.93DD256 pKa = 4.4SLLATGDD263 pKa = 3.65GLFIEE268 pKa = 5.27SVARR272 pKa = 11.84PDD274 pKa = 4.69LEE276 pKa = 4.65EE277 pKa = 6.06GDD279 pKa = 4.23EE280 pKa = 4.34GAPDD284 pKa = 4.07AARR287 pKa = 11.84MLYY290 pKa = 10.33EE291 pKa = 4.13SLQEE295 pKa = 4.15RR296 pKa = 11.84VLTLPDD302 pKa = 3.46DD303 pKa = 4.06TLVGGAHH310 pKa = 6.6FSGAAVAAEE319 pKa = 4.55DD320 pKa = 3.79GTYY323 pKa = 9.56TATIGEE329 pKa = 4.83LKK331 pKa = 9.98TRR333 pKa = 11.84MDD335 pKa = 4.34ALSMDD340 pKa = 3.82EE341 pKa = 4.69EE342 pKa = 4.83DD343 pKa = 5.56FVDD346 pKa = 6.74LILADD351 pKa = 3.98MPPRR355 pKa = 11.84PANYY359 pKa = 9.92EE360 pKa = 4.11DD361 pKa = 5.2IIATNLGQHH370 pKa = 6.35AVDD373 pKa = 4.37DD374 pKa = 4.79DD375 pKa = 4.19EE376 pKa = 6.15AFSLEE381 pKa = 4.58LGPNNCAASQEE392 pKa = 4.43SLAGDD397 pKa = 3.77

Molecular weight: 42.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3IDS0|A0A1H3IDS0_9EURY O-succinylbenzoic acid--CoA ligase OS=Halobellus clavatus OX=660517 GN=SAMN04487946_109124 PE=4 SV=1
MM1 pKa = 7.43SATPVVVALSLVSGAFWGVGPIFSKK26 pKa = 11.01LGMEE30 pKa = 4.4RR31 pKa = 11.84GGRR34 pKa = 11.84PHH36 pKa = 7.54RR37 pKa = 11.84ATLIVLVVGAAVFWAALLVRR57 pKa = 11.84GAGQASVARR66 pKa = 11.84LPAVDD71 pKa = 3.05ITVFAVSGVIGTSLAWLSWFRR92 pKa = 11.84GIRR95 pKa = 11.84LVGASVGNVLFYY107 pKa = 9.14TQPLFATLLAAAVLGEE123 pKa = 4.34RR124 pKa = 11.84LTSVTGVGVVLIVLGVGLLSLSGGSNLEE152 pKa = 3.81FRR154 pKa = 11.84LSKK157 pKa = 10.99ALLFPLAAAVFAAISNVVNRR177 pKa = 11.84FGFQTSTATPLEE189 pKa = 4.11AAAINLTSALPFIVGHH205 pKa = 4.85TLGYY209 pKa = 9.91HH210 pKa = 6.06RR211 pKa = 11.84NALGTPDD218 pKa = 4.38RR219 pKa = 11.84SDD221 pKa = 3.47AYY223 pKa = 9.62FVCSGLANAVAVFTMFTALANGPVVIVAPLVSTSPLFTTALAAVFLTDD271 pKa = 3.9VEE273 pKa = 4.88TITARR278 pKa = 11.84TVASAVLTVVGAGITAVV295 pKa = 3.22

Molecular weight: 30.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3679

0

3679

1088495

39

4622

295.9

32.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.89 ± 0.061

0.656 ± 0.013

8.367 ± 0.048

8.387 ± 0.059

3.286 ± 0.029

8.36 ± 0.043

1.953 ± 0.023

4.256 ± 0.032

1.845 ± 0.034

8.873 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.018

2.412 ± 0.031

4.708 ± 0.03

2.697 ± 0.023

6.634 ± 0.042

5.894 ± 0.037

6.548 ± 0.058

8.806 ± 0.046

1.122 ± 0.018

2.694 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski