Amino acid dipepetide frequency for Paenibacillus phage Paisley

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.949AlaAla: 3.949 ± 0.624
0.804AlaCys: 0.804 ± 0.23
3.437AlaAsp: 3.437 ± 0.646
4.972AlaGlu: 4.972 ± 0.647
2.34AlaPhe: 2.34 ± 0.503
4.314AlaGly: 4.314 ± 0.674
1.097AlaHis: 1.097 ± 0.271
4.46AlaIle: 4.46 ± 0.632
4.68AlaLys: 4.68 ± 0.627
6.8AlaLeu: 6.8 ± 1.278
1.536AlaMet: 1.536 ± 0.336
2.705AlaAsn: 2.705 ± 0.359
1.243AlaPro: 1.243 ± 0.311
2.632AlaGln: 2.632 ± 0.567
2.413AlaArg: 2.413 ± 0.539
3.437AlaSer: 3.437 ± 0.621
2.998AlaThr: 2.998 ± 0.662
4.387AlaVal: 4.387 ± 0.869
0.804AlaTrp: 0.804 ± 0.264
2.486AlaTyr: 2.486 ± 0.44
0.0AlaXaa: 0.0 ± 0.0
Cys
0.292CysAla: 0.292 ± 0.143
0.146CysCys: 0.146 ± 0.093
0.658CysAsp: 0.658 ± 0.247
1.024CysGlu: 1.024 ± 0.259
0.292CysPhe: 0.292 ± 0.156
0.877CysGly: 0.877 ± 0.306
0.366CysHis: 0.366 ± 0.176
0.512CysIle: 0.512 ± 0.205
0.658CysLys: 0.658 ± 0.266
0.877CysLeu: 0.877 ± 0.24
0.073CysMet: 0.073 ± 0.068
0.439CysAsn: 0.439 ± 0.193
0.512CysPro: 0.512 ± 0.237
0.292CysGln: 0.292 ± 0.13
0.585CysArg: 0.585 ± 0.229
0.366CysSer: 0.366 ± 0.171
0.439CysThr: 0.439 ± 0.191
0.512CysVal: 0.512 ± 0.243
0.146CysTrp: 0.146 ± 0.097
0.512CysTyr: 0.512 ± 0.165
0.0CysXaa: 0.0 ± 0.0
Asp
3.364AspAla: 3.364 ± 0.555
0.439AspCys: 0.439 ± 0.184
4.533AspAsp: 4.533 ± 0.55
5.557AspGlu: 5.557 ± 0.654
2.632AspPhe: 2.632 ± 0.37
5.118AspGly: 5.118 ± 0.763
1.17AspHis: 1.17 ± 0.265
3.583AspIle: 3.583 ± 0.562
3.217AspLys: 3.217 ± 0.531
4.899AspLeu: 4.899 ± 0.662
2.121AspMet: 2.121 ± 0.344
2.413AspAsn: 2.413 ± 0.591
1.974AspPro: 1.974 ± 0.455
2.267AspGln: 2.267 ± 0.498
2.632AspArg: 2.632 ± 0.464
2.632AspSer: 2.632 ± 0.588
2.852AspThr: 2.852 ± 0.469
3.875AspVal: 3.875 ± 0.601
0.951AspTrp: 0.951 ± 0.259
1.828AspTyr: 1.828 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
4.753GluAla: 4.753 ± 0.636
0.585GluCys: 0.585 ± 0.209
4.753GluAsp: 4.753 ± 0.6
6.142GluGlu: 6.142 ± 0.999
2.998GluPhe: 2.998 ± 0.318
3.875GluGly: 3.875 ± 0.534
0.877GluHis: 0.877 ± 0.268
5.777GluIle: 5.777 ± 0.682
6.873GluLys: 6.873 ± 0.814
7.824GluLeu: 7.824 ± 0.953
2.559GluMet: 2.559 ± 0.503
4.241GluAsn: 4.241 ± 0.601
1.974GluPro: 1.974 ± 0.424
4.533GluGln: 4.533 ± 0.611
4.168GluArg: 4.168 ± 0.572
3.656GluSer: 3.656 ± 0.598
4.022GluThr: 4.022 ± 0.526
5.045GluVal: 5.045 ± 0.665
0.658GluTrp: 0.658 ± 0.265
3.071GluTyr: 3.071 ± 0.477
0.0GluXaa: 0.0 ± 0.0
Phe
2.34PheAla: 2.34 ± 0.612
0.951PheCys: 0.951 ± 0.229
2.998PheAsp: 2.998 ± 0.486
2.779PheGlu: 2.779 ± 0.473
1.389PhePhe: 1.389 ± 0.381
2.559PheGly: 2.559 ± 0.43
1.024PheHis: 1.024 ± 0.27
2.998PheIle: 2.998 ± 0.484
2.852PheLys: 2.852 ± 0.458
3.364PheLeu: 3.364 ± 0.426
0.877PheMet: 0.877 ± 0.344
2.121PheAsn: 2.121 ± 0.388
1.462PhePro: 1.462 ± 0.343
1.536PheGln: 1.536 ± 0.443
1.243PheArg: 1.243 ± 0.397
3.51PheSer: 3.51 ± 0.708
2.779PheThr: 2.779 ± 0.429
2.705PheVal: 2.705 ± 0.496
0.366PheTrp: 0.366 ± 0.166
1.389PheTyr: 1.389 ± 0.31
0.0PheXaa: 0.0 ± 0.0
Gly
3.29GlyAla: 3.29 ± 0.722
0.804GlyCys: 0.804 ± 0.224
4.095GlyAsp: 4.095 ± 0.636
5.338GlyGlu: 5.338 ± 0.854
2.121GlyPhe: 2.121 ± 0.355
4.387GlyGly: 4.387 ± 0.771
0.658GlyHis: 0.658 ± 0.244
5.703GlyIle: 5.703 ± 1.087
5.777GlyLys: 5.777 ± 0.671
4.826GlyLeu: 4.826 ± 0.576
1.682GlyMet: 1.682 ± 0.334
3.802GlyAsn: 3.802 ± 0.508
1.024GlyPro: 1.024 ± 0.288
2.267GlyGln: 2.267 ± 0.409
3.29GlyArg: 3.29 ± 0.63
2.852GlySer: 2.852 ± 0.499
2.925GlyThr: 2.925 ± 0.516
3.729GlyVal: 3.729 ± 0.645
0.804GlyTrp: 0.804 ± 0.232
2.413GlyTyr: 2.413 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
0.804HisAla: 0.804 ± 0.198
0.366HisCys: 0.366 ± 0.158
0.439HisAsp: 0.439 ± 0.153
1.316HisGlu: 1.316 ± 0.343
0.951HisPhe: 0.951 ± 0.246
0.951HisGly: 0.951 ± 0.355
0.292HisHis: 0.292 ± 0.156
0.951HisIle: 0.951 ± 0.264
0.951HisLys: 0.951 ± 0.3
2.705HisLeu: 2.705 ± 0.51
0.658HisMet: 0.658 ± 0.209
0.951HisAsn: 0.951 ± 0.267
0.658HisPro: 0.658 ± 0.283
0.439HisGln: 0.439 ± 0.177
0.804HisArg: 0.804 ± 0.239
0.731HisSer: 0.731 ± 0.224
0.877HisThr: 0.877 ± 0.349
1.389HisVal: 1.389 ± 0.289
0.366HisTrp: 0.366 ± 0.164
0.439HisTyr: 0.439 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.168IleAla: 4.168 ± 1.047
0.731IleCys: 0.731 ± 0.251
4.022IleAsp: 4.022 ± 0.682
6.288IleGlu: 6.288 ± 0.695
3.071IlePhe: 3.071 ± 0.447
4.314IleGly: 4.314 ± 0.644
1.097IleHis: 1.097 ± 0.313
4.972IleIle: 4.972 ± 0.783
7.751IleLys: 7.751 ± 0.77
5.85IleLeu: 5.85 ± 0.67
1.755IleMet: 1.755 ± 0.474
3.802IleAsn: 3.802 ± 0.616
2.998IlePro: 2.998 ± 0.59
3.729IleGln: 3.729 ± 0.499
3.583IleArg: 3.583 ± 0.574
5.045IleSer: 5.045 ± 0.757
4.022IleThr: 4.022 ± 0.46
3.875IleVal: 3.875 ± 0.561
0.731IleTrp: 0.731 ± 0.22
1.828IleTyr: 1.828 ± 0.346
0.0IleXaa: 0.0 ± 0.0
Lys
5.996LysAla: 5.996 ± 0.845
0.366LysCys: 0.366 ± 0.214
4.68LysAsp: 4.68 ± 0.659
6.654LysGlu: 6.654 ± 0.807
2.559LysPhe: 2.559 ± 0.345
5.192LysGly: 5.192 ± 0.678
1.462LysHis: 1.462 ± 0.34
5.557LysIle: 5.557 ± 0.538
6.873LysLys: 6.873 ± 1.022
7.093LysLeu: 7.093 ± 0.642
2.486LysMet: 2.486 ± 0.372
4.68LysAsn: 4.68 ± 0.482
1.974LysPro: 1.974 ± 0.453
3.583LysGln: 3.583 ± 0.613
5.484LysArg: 5.484 ± 0.792
4.387LysSer: 4.387 ± 0.535
5.484LysThr: 5.484 ± 0.623
5.045LysVal: 5.045 ± 0.702
1.389LysTrp: 1.389 ± 0.281
2.559LysTyr: 2.559 ± 0.541
0.0LysXaa: 0.0 ± 0.0
Leu
5.923LeuAla: 5.923 ± 0.732
0.731LeuCys: 0.731 ± 0.234
6.069LeuAsp: 6.069 ± 0.613
6.508LeuGlu: 6.508 ± 0.647
4.314LeuPhe: 4.314 ± 0.594
5.192LeuGly: 5.192 ± 0.932
1.316LeuHis: 1.316 ± 0.293
6.654LeuIle: 6.654 ± 0.932
6.8LeuLys: 6.8 ± 0.641
6.069LeuLeu: 6.069 ± 0.748
2.267LeuMet: 2.267 ± 0.44
4.753LeuAsn: 4.753 ± 0.512
4.022LeuPro: 4.022 ± 0.473
3.949LeuGln: 3.949 ± 0.592
4.533LeuArg: 4.533 ± 0.579
5.484LeuSer: 5.484 ± 0.662
4.46LeuThr: 4.46 ± 0.465
4.022LeuVal: 4.022 ± 0.449
0.804LeuTrp: 0.804 ± 0.24
3.071LeuTyr: 3.071 ± 0.535
0.0LeuXaa: 0.0 ± 0.0
Met
2.121MetAla: 2.121 ± 0.413
0.219MetCys: 0.219 ± 0.121
1.243MetAsp: 1.243 ± 0.343
1.901MetGlu: 1.901 ± 0.378
1.536MetPhe: 1.536 ± 0.332
1.389MetGly: 1.389 ± 0.36
0.219MetHis: 0.219 ± 0.114
2.047MetIle: 2.047 ± 0.416
2.925MetLys: 2.925 ± 0.42
2.559MetLeu: 2.559 ± 0.577
0.731MetMet: 0.731 ± 0.184
2.047MetAsn: 2.047 ± 0.438
0.731MetPro: 0.731 ± 0.243
1.097MetGln: 1.097 ± 0.268
1.17MetArg: 1.17 ± 0.289
1.828MetSer: 1.828 ± 0.335
1.389MetThr: 1.389 ± 0.311
0.877MetVal: 0.877 ± 0.228
0.585MetTrp: 0.585 ± 0.179
1.024MetTyr: 1.024 ± 0.285
0.0MetXaa: 0.0 ± 0.0
Asn
3.729AsnAla: 3.729 ± 0.631
0.219AsnCys: 0.219 ± 0.097
2.267AsnAsp: 2.267 ± 0.534
3.656AsnGlu: 3.656 ± 0.583
1.682AsnPhe: 1.682 ± 0.304
3.656AsnGly: 3.656 ± 0.635
1.316AsnHis: 1.316 ± 0.351
2.925AsnIle: 2.925 ± 0.461
4.022AsnLys: 4.022 ± 0.577
4.387AsnLeu: 4.387 ± 0.487
1.536AsnMet: 1.536 ± 0.375
2.779AsnAsn: 2.779 ± 0.525
2.267AsnPro: 2.267 ± 0.436
2.925AsnGln: 2.925 ± 0.559
2.852AsnArg: 2.852 ± 0.485
3.656AsnSer: 3.656 ± 0.515
2.194AsnThr: 2.194 ± 0.376
2.998AsnVal: 2.998 ± 0.481
0.658AsnTrp: 0.658 ± 0.208
2.121AsnTyr: 2.121 ± 0.355
0.0AsnXaa: 0.0 ± 0.0
Pro
2.413ProAla: 2.413 ± 0.445
0.219ProCys: 0.219 ± 0.131
2.705ProAsp: 2.705 ± 0.589
2.559ProGlu: 2.559 ± 0.469
1.536ProPhe: 1.536 ± 0.384
2.121ProGly: 2.121 ± 0.398
1.097ProHis: 1.097 ± 0.297
2.486ProIle: 2.486 ± 0.508
2.34ProLys: 2.34 ± 0.523
2.486ProLeu: 2.486 ± 0.547
0.658ProMet: 0.658 ± 0.307
2.267ProAsn: 2.267 ± 0.486
1.097ProPro: 1.097 ± 0.28
0.658ProGln: 0.658 ± 0.253
1.243ProArg: 1.243 ± 0.233
2.486ProSer: 2.486 ± 0.472
1.682ProThr: 1.682 ± 0.425
2.267ProVal: 2.267 ± 0.496
0.585ProTrp: 0.585 ± 0.203
1.097ProTyr: 1.097 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
3.729GlnAla: 3.729 ± 0.588
0.219GlnCys: 0.219 ± 0.112
1.462GlnAsp: 1.462 ± 0.289
2.632GlnGlu: 2.632 ± 0.478
1.536GlnPhe: 1.536 ± 0.353
2.413GlnGly: 2.413 ± 0.404
0.512GlnHis: 0.512 ± 0.196
3.51GlnIle: 3.51 ± 0.488
3.656GlnLys: 3.656 ± 0.563
4.168GlnLeu: 4.168 ± 0.534
1.536GlnMet: 1.536 ± 0.37
1.974GlnAsn: 1.974 ± 0.383
1.389GlnPro: 1.389 ± 0.334
1.17GlnGln: 1.17 ± 0.364
2.267GlnArg: 2.267 ± 0.475
1.755GlnSer: 1.755 ± 0.345
2.194GlnThr: 2.194 ± 0.332
2.925GlnVal: 2.925 ± 0.385
0.292GlnTrp: 0.292 ± 0.144
0.731GlnTyr: 0.731 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
2.121ArgAla: 2.121 ± 0.379
0.439ArgCys: 0.439 ± 0.183
2.267ArgAsp: 2.267 ± 0.395
3.875ArgGlu: 3.875 ± 0.681
2.047ArgPhe: 2.047 ± 0.366
2.559ArgGly: 2.559 ± 0.562
0.585ArgHis: 0.585 ± 0.213
4.095ArgIle: 4.095 ± 0.577
5.045ArgLys: 5.045 ± 0.733
4.314ArgLeu: 4.314 ± 0.666
1.17ArgMet: 1.17 ± 0.354
2.705ArgAsn: 2.705 ± 0.524
2.34ArgPro: 2.34 ± 0.392
1.901ArgGln: 1.901 ± 0.355
2.559ArgArg: 2.559 ± 0.424
2.705ArgSer: 2.705 ± 0.51
2.486ArgThr: 2.486 ± 0.482
2.852ArgVal: 2.852 ± 0.57
1.097ArgTrp: 1.097 ± 0.329
2.486ArgTyr: 2.486 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
3.583SerAla: 3.583 ± 0.703
0.146SerCys: 0.146 ± 0.107
2.413SerAsp: 2.413 ± 0.483
4.899SerGlu: 4.899 ± 0.693
3.144SerPhe: 3.144 ± 0.482
3.875SerGly: 3.875 ± 0.697
0.585SerHis: 0.585 ± 0.222
3.802SerIle: 3.802 ± 0.402
5.338SerLys: 5.338 ± 0.69
5.045SerLeu: 5.045 ± 0.685
1.682SerMet: 1.682 ± 0.315
3.144SerAsn: 3.144 ± 0.692
2.413SerPro: 2.413 ± 0.375
1.682SerGln: 1.682 ± 0.452
3.071SerArg: 3.071 ± 0.523
4.607SerSer: 4.607 ± 0.837
3.875SerThr: 3.875 ± 0.517
3.802SerVal: 3.802 ± 0.552
0.804SerTrp: 0.804 ± 0.248
2.121SerTyr: 2.121 ± 0.419
0.0SerXaa: 0.0 ± 0.0
Thr
3.144ThrAla: 3.144 ± 0.529
0.585ThrCys: 0.585 ± 0.235
2.925ThrAsp: 2.925 ± 0.441
3.656ThrGlu: 3.656 ± 0.621
2.267ThrPhe: 2.267 ± 0.417
4.022ThrGly: 4.022 ± 0.604
1.024ThrHis: 1.024 ± 0.263
4.533ThrIle: 4.533 ± 0.757
3.802ThrLys: 3.802 ± 0.527
4.314ThrLeu: 4.314 ± 0.576
1.243ThrMet: 1.243 ± 0.33
2.194ThrAsn: 2.194 ± 0.396
2.34ThrPro: 2.34 ± 0.465
1.828ThrGln: 1.828 ± 0.349
1.901ThrArg: 1.901 ± 0.388
3.656ThrSer: 3.656 ± 0.54
2.998ThrThr: 2.998 ± 0.488
4.095ThrVal: 4.095 ± 0.521
0.658ThrTrp: 0.658 ± 0.218
2.047ThrTyr: 2.047 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
3.217ValAla: 3.217 ± 0.46
1.097ValCys: 1.097 ± 0.314
3.729ValAsp: 3.729 ± 0.468
4.387ValGlu: 4.387 ± 0.649
2.779ValPhe: 2.779 ± 0.503
2.559ValGly: 2.559 ± 0.403
1.097ValHis: 1.097 ± 0.29
5.63ValIle: 5.63 ± 0.922
5.703ValLys: 5.703 ± 0.556
4.753ValLeu: 4.753 ± 0.468
1.974ValMet: 1.974 ± 0.376
2.779ValAsn: 2.779 ± 0.386
2.267ValPro: 2.267 ± 0.328
1.609ValGln: 1.609 ± 0.35
2.998ValArg: 2.998 ± 0.483
5.045ValSer: 5.045 ± 0.529
3.51ValThr: 3.51 ± 0.662
4.241ValVal: 4.241 ± 0.666
1.316ValTrp: 1.316 ± 0.293
1.974ValTyr: 1.974 ± 0.369
0.0ValXaa: 0.0 ± 0.0
Trp
0.731TrpAla: 0.731 ± 0.226
0.219TrpCys: 0.219 ± 0.128
0.804TrpAsp: 0.804 ± 0.245
0.951TrpGlu: 0.951 ± 0.22
0.877TrpPhe: 0.877 ± 0.268
0.366TrpGly: 0.366 ± 0.167
0.585TrpHis: 0.585 ± 0.18
0.804TrpIle: 0.804 ± 0.319
1.462TrpLys: 1.462 ± 0.358
1.536TrpLeu: 1.536 ± 0.327
0.292TrpMet: 0.292 ± 0.142
0.658TrpAsn: 0.658 ± 0.22
0.219TrpPro: 0.219 ± 0.139
0.366TrpGln: 0.366 ± 0.174
0.877TrpArg: 0.877 ± 0.201
0.804TrpSer: 0.804 ± 0.243
0.146TrpThr: 0.146 ± 0.097
0.951TrpVal: 0.951 ± 0.282
0.292TrpTrp: 0.292 ± 0.157
0.731TrpTyr: 0.731 ± 0.23
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.828TyrAla: 1.828 ± 0.334
0.292TyrCys: 0.292 ± 0.151
2.559TyrAsp: 2.559 ± 0.497
3.29TyrGlu: 3.29 ± 0.42
1.17TyrPhe: 1.17 ± 0.264
2.047TyrGly: 2.047 ± 0.392
0.658TyrHis: 0.658 ± 0.262
2.632TyrIle: 2.632 ± 0.526
2.779TyrLys: 2.779 ± 0.51
3.071TyrLeu: 3.071 ± 0.473
0.731TyrMet: 0.731 ± 0.238
1.389TyrAsn: 1.389 ± 0.346
1.097TyrPro: 1.097 ± 0.309
1.609TyrGln: 1.609 ± 0.394
2.047TyrArg: 2.047 ± 0.372
1.389TyrSer: 1.389 ± 0.304
1.901TyrThr: 1.901 ± 0.445
3.071TyrVal: 3.071 ± 0.552
0.366TyrTrp: 0.366 ± 0.193
1.609TyrTyr: 1.609 ± 0.323
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (13677 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski