Geobacillus kaustophilus (strain HTA426)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; Geobacillus thermoleovorans group; Geobacillus kaustophilus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3516 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5KY38|Q5KY38_GEOKA Hypothetical conserved protein OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=GK2113 PE=1 SV=1
MM1 pKa = 8.27DD2 pKa = 6.62DD3 pKa = 4.73LLQCIEE9 pKa = 5.42DD10 pKa = 4.1DD11 pKa = 5.01LEE13 pKa = 5.91GNLPPEE19 pKa = 4.0QFSYY23 pKa = 11.24DD24 pKa = 3.48FPAIYY29 pKa = 10.13ASYY32 pKa = 10.74FDD34 pKa = 6.23DD35 pKa = 5.14GDD37 pKa = 4.15LDD39 pKa = 3.83EE40 pKa = 6.21KK41 pKa = 11.44YY42 pKa = 10.42IDD44 pKa = 3.99AFDD47 pKa = 6.04DD48 pKa = 3.47ISEE51 pKa = 4.28ACGWYY56 pKa = 9.83EE57 pKa = 3.7PNPLHH62 pKa = 7.57RR63 pKa = 11.84EE64 pKa = 3.92DD65 pKa = 4.05DD66 pKa = 3.68DD67 pKa = 4.75EE68 pKa = 5.28YY69 pKa = 10.89IGEE72 pKa = 4.08EE73 pKa = 3.85EE74 pKa = 4.33LRR76 pKa = 11.84NKK78 pKa = 9.88VEE80 pKa = 4.55EE81 pKa = 4.44KK82 pKa = 10.3YY83 pKa = 7.95QTIKK87 pKa = 10.86KK88 pKa = 10.18LSTRR92 pKa = 11.84STT94 pKa = 3.42

Molecular weight: 11.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5KU70|Q5KU70_GEOKA Single-stranded DNA-binding protein OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=GK3481 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84SKK14 pKa = 9.65VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.3VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3516

0

3516

1005871

23

2301

286.1

32.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.226 ± 0.054

0.862 ± 0.014

4.862 ± 0.031

7.419 ± 0.046

4.168 ± 0.028

7.246 ± 0.037

2.308 ± 0.023

6.379 ± 0.04

5.631 ± 0.036

9.939 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.664 ± 0.021

3.101 ± 0.025

4.283 ± 0.027

3.652 ± 0.027

6.075 ± 0.039

4.923 ± 0.029

4.978 ± 0.028

7.627 ± 0.033

1.292 ± 0.02

3.364 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski