Enterococcus phage BC611

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; Enterococcus virus BC611

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0IS72|K0IS72_9CAUD Uncharacterized protein OS=Enterococcus phage BC611 OX=1173135 PE=4 SV=1
MM1 pKa = 7.91IEE3 pKa = 4.22LFKK6 pKa = 11.43EE7 pKa = 3.93NLTLDD12 pKa = 3.64EE13 pKa = 5.54MSDD16 pKa = 2.78IWYY19 pKa = 9.05GWDD22 pKa = 4.22NEE24 pKa = 3.9GLLYY28 pKa = 10.6GWEE31 pKa = 3.94NGEE34 pKa = 4.07AGEE37 pKa = 4.79AYY39 pKa = 10.4ALLYY43 pKa = 10.03EE44 pKa = 4.79KK45 pKa = 10.14GYY47 pKa = 11.44NMDD50 pKa = 4.11SIKK53 pKa = 10.61VLHH56 pKa = 6.42EE57 pKa = 4.29AFDD60 pKa = 4.45TIGGLIGAII69 pKa = 3.72

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0IX54|K0IX54_9CAUD Uncharacterized protein OS=Enterococcus phage BC611 OX=1173135 PE=4 SV=1
MM1 pKa = 7.1NVEE4 pKa = 4.22SRR6 pKa = 11.84NSEE9 pKa = 4.24KK10 pKa = 10.57AWNRR14 pKa = 11.84AIVKK18 pKa = 8.88TEE20 pKa = 3.5QEE22 pKa = 3.9AAKK25 pKa = 10.67KK26 pKa = 10.42KK27 pKa = 8.64MEE29 pKa = 4.46RR30 pKa = 11.84LAKK33 pKa = 9.42MRR35 pKa = 11.84AKK37 pKa = 10.47AKK39 pKa = 10.39RR40 pKa = 11.84KK41 pKa = 9.4

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15598

33

1331

179.3

20.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.437 ± 0.409

0.654 ± 0.087

6.084 ± 0.214

8.719 ± 0.431

4.071 ± 0.202

6.18 ± 0.427

1.513 ± 0.133

6.257 ± 0.239

7.911 ± 0.3

8.463 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.629 ± 0.195

5.225 ± 0.26

2.949 ± 0.228

3.558 ± 0.285

4.257 ± 0.18

6.02 ± 0.208

6.065 ± 0.367

6.578 ± 0.237

1.225 ± 0.11

4.206 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski