Mycoplasma sp. CAG:776

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; environmental samples

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7NI99|R7NI99_9MOLU Uncharacterized protein OS=Mycoplasma sp. CAG:776 OX=1262906 GN=BN778_00104 PE=4 SV=1
MM1 pKa = 7.31NKK3 pKa = 9.86EE4 pKa = 4.36DD5 pKa = 5.82IITLEE10 pKa = 4.26DD11 pKa = 3.33NVEE14 pKa = 4.0YY15 pKa = 9.99MVLDD19 pKa = 3.59IATINQEE26 pKa = 3.79KK27 pKa = 10.82YY28 pKa = 9.93LFCVEE33 pKa = 3.87IDD35 pKa = 3.68EE36 pKa = 5.53EE37 pKa = 4.32EE38 pKa = 4.22MPKK41 pKa = 10.83NNYY44 pKa = 10.05KK45 pKa = 10.29YY46 pKa = 9.63FQEE49 pKa = 4.13INEE52 pKa = 4.35SNNIFVEE59 pKa = 4.44EE60 pKa = 4.64VEE62 pKa = 4.3DD63 pKa = 3.78QDD65 pKa = 4.25VIEE68 pKa = 5.3AISAIFTANHH78 pKa = 6.57LNNITNGEE86 pKa = 3.9QDD88 pKa = 3.2VV89 pKa = 3.66

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7NI04|R7NI04_9MOLU Oxidoreductase short chain dehydrogenase/reductase family OS=Mycoplasma sp. CAG:776 OX=1262906 GN=BN778_00133 PE=3 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.3KK4 pKa = 10.89SMIIKK9 pKa = 10.3NKK11 pKa = 8.54RR12 pKa = 11.84TPKK15 pKa = 9.47YY16 pKa = 9.82KK17 pKa = 9.34VRR19 pKa = 11.84AYY21 pKa = 8.38TRR23 pKa = 11.84CEE25 pKa = 3.71RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 6.48GVLRR35 pKa = 11.84KK36 pKa = 9.72FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.85LANEE50 pKa = 4.09GKK52 pKa = 10.11IPGVKK57 pKa = 9.79KK58 pKa = 10.91SSWW61 pKa = 3.1

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1349

0

1349

384452

29

2078

285.0

32.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.604 ± 0.052

1.024 ± 0.022

5.651 ± 0.068

7.892 ± 0.089

4.457 ± 0.059

5.392 ± 0.072

1.5 ± 0.032

10.024 ± 0.085

9.236 ± 0.08

9.724 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.03

6.979 ± 0.082

2.494 ± 0.034

2.289 ± 0.033

3.165 ± 0.046

6.1 ± 0.058

5.367 ± 0.075

5.874 ± 0.057

0.599 ± 0.017

5.284 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski