Azospirillum phage Cd

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0VK78|B0VK78_9CAUD Uncharacterized protein 78 OS=Azospirillum phage Cd OX=467481 GN=78 PE=4 SV=1
MM1 pKa = 7.01TTTDD5 pKa = 3.61MQTKK9 pKa = 10.11SNSTFEE15 pKa = 3.6QWMNGVAAMWGMTASNLEE33 pKa = 3.84ADD35 pKa = 4.01EE36 pKa = 3.88WRR38 pKa = 11.84PYY40 pKa = 10.52YY41 pKa = 10.41DD42 pKa = 3.27AGYY45 pKa = 10.53DD46 pKa = 3.24HH47 pKa = 7.29GEE49 pKa = 3.78ALEE52 pKa = 4.45KK53 pKa = 10.47IKK55 pKa = 11.2AGDD58 pKa = 3.53EE59 pKa = 4.08PP60 pKa = 4.58

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B0VK12|B0VK12_9CAUD Uncharacterized protein 12 OS=Azospirillum phage Cd OX=467481 GN=12 PE=4 SV=1
MM1 pKa = 7.33SASPNPVADD10 pKa = 4.07LLRR13 pKa = 11.84FRR15 pKa = 11.84EE16 pKa = 4.12RR17 pKa = 11.84LKK19 pKa = 10.77SARR22 pKa = 11.84IDD24 pKa = 3.46QKK26 pKa = 9.03MTQGDD31 pKa = 3.62VARR34 pKa = 11.84RR35 pKa = 11.84MGKK38 pKa = 9.59DD39 pKa = 2.95PRR41 pKa = 11.84TVRR44 pKa = 11.84RR45 pKa = 11.84WEE47 pKa = 4.02NNEE50 pKa = 3.91GEE52 pKa = 4.25PSLTEE57 pKa = 3.66AALWAHH63 pKa = 6.03AVGVQMLPPVPFDD76 pKa = 3.67LVARR80 pKa = 11.84AAGGRR85 pKa = 11.84GRR87 pKa = 11.84TKK89 pKa = 10.48RR90 pKa = 11.84DD91 pKa = 3.36ALCAMQAAVNADD103 pKa = 3.99LAQTRR108 pKa = 11.84ASNLKK113 pKa = 10.45GSATAA118 pKa = 4.6

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17952

49

1462

189.0

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.675 ± 0.482

1.214 ± 0.142

5.938 ± 0.24

5.66 ± 0.27

2.741 ± 0.151

8.5 ± 0.283

1.9 ± 0.176

3.988 ± 0.148

4.228 ± 0.291

8.211 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.707 ± 0.142

2.852 ± 0.172

4.98 ± 0.311

3.326 ± 0.239

7.442 ± 0.327

5.888 ± 0.29

6.239 ± 0.351

6.551 ± 0.225

1.861 ± 0.112

2.1 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski