Amino acid dipepetide frequency for Anaerobacillus isosaccharinicus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.501AlaAla: 4.501 ± 0.068	0.646AlaCys: 0.646 ± 0.02	2.857AlaAsp: 2.857 ± 0.047	4.1AlaGlu: 4.1 ± 0.063	3.173AlaPhe: 3.173 ± 0.044	4.226AlaGly: 4.226 ± 0.067	1.178AlaHis: 1.178 ± 0.028	5.876AlaIle: 5.876 ± 0.072	4.622AlaLys: 4.622 ± 0.064	6.691AlaLeu: 6.691 ± 0.078	1.898AlaMet: 1.898 ± 0.039	2.874AlaAsn: 2.874 ± 0.048	1.839AlaPro: 1.839 ± 0.043	2.032AlaGln: 2.032 ± 0.043	2.435AlaArg: 2.435 ± 0.046	3.799AlaSer: 3.799 ± 0.059	3.599AlaThr: 3.599 ± 0.054	4.648AlaVal: 4.648 ± 0.064	0.539AlaTrp: 0.539 ± 0.022	2.08AlaTyr: 2.08 ± 0.046	0.007AlaXaa: 0.007 ± 0.003
Cys	0.415CysAla: 0.415 ± 0.017	0.122CysCys: 0.122 ± 0.01	0.443CysAsp: 0.443 ± 0.019	0.522CysGlu: 0.522 ± 0.017	0.367CysPhe: 0.367 ± 0.017	0.737CysGly: 0.737 ± 0.022	0.281CysHis: 0.281 ± 0.021	0.592CysIle: 0.592 ± 0.021	0.491CysLys: 0.491 ± 0.018	0.773CysLeu: 0.773 ± 0.022	0.174CysMet: 0.174 ± 0.01	0.348CysAsn: 0.348 ± 0.015	0.476CysPro: 0.476 ± 0.022	0.275CysGln: 0.275 ± 0.014	0.33CysArg: 0.33 ± 0.017	0.671CysSer: 0.671 ± 0.024	0.403CysThr: 0.403 ± 0.017	0.449CysVal: 0.449 ± 0.018	0.076CysTrp: 0.076 ± 0.007	0.285CysTyr: 0.285 ± 0.013	0.001CysXaa: 0.001 ± 0.001
Asp	2.922AspAla: 2.922 ± 0.043	0.439AspCys: 0.439 ± 0.018	2.465AspAsp: 2.465 ± 0.048	4.359AspGlu: 4.359 ± 0.066	2.479AspPhe: 2.479 ± 0.047	3.193AspGly: 3.193 ± 0.05	1.252AspHis: 1.252 ± 0.03	3.929AspIle: 3.929 ± 0.058	3.078AspLys: 3.078 ± 0.054	5.159AspLeu: 5.159 ± 0.061	1.188AspMet: 1.188 ± 0.03	1.863AspAsn: 1.863 ± 0.037	1.9AspPro: 1.9 ± 0.039	1.997AspGln: 1.997 ± 0.039	2.183AspArg: 2.183 ± 0.034	2.271AspSer: 2.271 ± 0.043	2.172AspThr: 2.172 ± 0.041	3.709AspVal: 3.709 ± 0.052	0.606AspTrp: 0.606 ± 0.021	2.148AspTyr: 2.148 ± 0.043	0.003AspXaa: 0.003 ± 0.002
Glu	5.237GluAla: 5.237 ± 0.073	0.43GluCys: 0.43 ± 0.016	3.776GluAsp: 3.776 ± 0.057	7.1GluGlu: 7.1 ± 0.09	2.708GluPhe: 2.708 ± 0.038	4.2GluGly: 4.2 ± 0.057	1.473GluHis: 1.473 ± 0.029	6.166GluIle: 6.166 ± 0.071	7.081GluLys: 7.081 ± 0.078	7.471GluLeu: 7.471 ± 0.082	2.224GluMet: 2.224 ± 0.043	3.906GluAsn: 3.906 ± 0.053	1.921GluPro: 1.921 ± 0.044	3.289GluGln: 3.289 ± 0.059	3.537GluArg: 3.537 ± 0.058	3.018GluSer: 3.018 ± 0.046	4.025GluThr: 4.025 ± 0.054	5.572GluVal: 5.572 ± 0.069	0.774GluTrp: 0.774 ± 0.025	2.176GluTyr: 2.176 ± 0.038	0.006GluXaa: 0.006 ± 0.002
Phe	2.867PheAla: 2.867 ± 0.037	0.425PheCys: 0.425 ± 0.016	2.394PheAsp: 2.394 ± 0.042	3.064PheGlu: 3.064 ± 0.045	2.708PhePhe: 2.708 ± 0.06	3.287PheGly: 3.287 ± 0.051	1.057PheHis: 1.057 ± 0.031	4.243PheIle: 4.243 ± 0.067	2.568PheLys: 2.568 ± 0.049	5.066PheLeu: 5.066 ± 0.081	1.217PheMet: 1.217 ± 0.028	2.047PheAsn: 2.047 ± 0.04	1.688PhePro: 1.688 ± 0.039	1.827PheGln: 1.827 ± 0.04	1.655PheArg: 1.655 ± 0.032	3.423PheSer: 3.423 ± 0.056	2.662PheThr: 2.662 ± 0.045	3.345PheVal: 3.345 ± 0.052	0.497PheTrp: 0.497 ± 0.018	1.873PheTyr: 1.873 ± 0.042	0.004PheXaa: 0.004 ± 0.002
Gly	4.289GlyAla: 4.289 ± 0.073	0.654GlyCys: 0.654 ± 0.022	3.138GlyAsp: 3.138 ± 0.049	4.417GlyGlu: 4.417 ± 0.061	3.434GlyPhe: 3.434 ± 0.055	4.373GlyGly: 4.373 ± 0.062	1.385GlyHis: 1.385 ± 0.03	5.781GlyIle: 5.781 ± 0.076	4.798GlyLys: 4.798 ± 0.063	6.33GlyLeu: 6.33 ± 0.069	1.826GlyMet: 1.826 ± 0.035	2.793GlyAsn: 2.793 ± 0.047	1.671GlyPro: 1.671 ± 0.039	2.078GlyGln: 2.078 ± 0.044	2.505GlyArg: 2.505 ± 0.048	3.655GlySer: 3.655 ± 0.055	3.796GlyThr: 3.796 ± 0.054	4.845GlyVal: 4.845 ± 0.061	0.735GlyTrp: 0.735 ± 0.022	2.637GlyTyr: 2.637 ± 0.043	0.003GlyXaa: 0.003 ± 0.001
His	1.122HisAla: 1.122 ± 0.026	0.237HisCys: 0.237 ± 0.014	1.06HisAsp: 1.06 ± 0.027	1.429HisGlu: 1.429 ± 0.032	1.138HisPhe: 1.138 ± 0.028	1.378HisGly: 1.378 ± 0.037	0.676HisHis: 0.676 ± 0.022	1.486HisIle: 1.486 ± 0.031	1.057HisLys: 1.057 ± 0.026	2.244HisLeu: 2.244 ± 0.038	0.494HisMet: 0.494 ± 0.02	0.823HisAsn: 0.823 ± 0.02	1.076HisPro: 1.076 ± 0.027	0.836HisGln: 0.836 ± 0.025	0.855HisArg: 0.855 ± 0.026	1.295HisSer: 1.295 ± 0.03	0.964HisThr: 0.964 ± 0.023	1.43HisVal: 1.43 ± 0.03	0.242HisTrp: 0.242 ± 0.013	0.998HisTyr: 0.998 ± 0.031	0.001HisXaa: 0.001 ± 0.001
Ile	5.681IleAla: 5.681 ± 0.066	0.732IleCys: 0.732 ± 0.023	4.629IleAsp: 4.629 ± 0.061	6.369IleGlu: 6.369 ± 0.073	3.731IlePhe: 3.731 ± 0.061	5.882IleGly: 5.882 ± 0.075	1.63IleHis: 1.63 ± 0.038	6.808IleIle: 6.808 ± 0.084	5.105IleLys: 5.105 ± 0.068	7.44IleLeu: 7.44 ± 0.079	1.781IleMet: 1.781 ± 0.033	3.909IleAsn: 3.909 ± 0.053	3.231IlePro: 3.231 ± 0.047	2.709IleGln: 2.709 ± 0.043	3.059IleArg: 3.059 ± 0.05	5.574IleSer: 5.574 ± 0.07	4.704IleThr: 4.704 ± 0.063	5.829IleVal: 5.829 ± 0.062	0.672IleTrp: 0.672 ± 0.022	2.626IleTyr: 2.626 ± 0.04	0.004IleXaa: 0.004 ± 0.002
Lys	4.478LysAla: 4.478 ± 0.054	0.426LysCys: 0.426 ± 0.015	3.583LysAsp: 3.583 ± 0.05	6.904LysGlu: 6.904 ± 0.077	2.179LysPhe: 2.179 ± 0.039	4.437LysGly: 4.437 ± 0.053	1.389LysHis: 1.389 ± 0.032	5.19LysIle: 5.19 ± 0.062	6.569LysLys: 6.569 ± 0.078	6.601LysLeu: 6.601 ± 0.069	2.112LysMet: 2.112 ± 0.04	3.649LysAsn: 3.649 ± 0.052	2.198LysPro: 2.198 ± 0.045	3.259LysGln: 3.259 ± 0.05	3.395LysArg: 3.395 ± 0.049	3.597LysSer: 3.597 ± 0.048	3.74LysThr: 3.74 ± 0.052	4.939LysVal: 4.939 ± 0.066	0.776LysTrp: 0.776 ± 0.02	2.32LysTyr: 2.32 ± 0.039	0.011LysXaa: 0.011 ± 0.003
Leu	7.101LeuAla: 7.101 ± 0.069	0.713LeuCys: 0.713 ± 0.022	4.778LeuAsp: 4.778 ± 0.058	7.109LeuGlu: 7.109 ± 0.076	5.075LeuPhe: 5.075 ± 0.066	6.312LeuGly: 6.312 ± 0.074	1.975LeuHis: 1.975 ± 0.04	7.815LeuIle: 7.815 ± 0.089	7.113LeuLys: 7.113 ± 0.072	10.599LeuLeu: 10.599 ± 0.113	2.431LeuMet: 2.431 ± 0.049	4.825LeuAsn: 4.825 ± 0.06	3.773LeuPro: 3.773 ± 0.059	3.634LeuGln: 3.634 ± 0.052	3.791LeuArg: 3.791 ± 0.056	6.655LeuSer: 6.655 ± 0.067	5.836LeuThr: 5.836 ± 0.069	6.518LeuVal: 6.518 ± 0.062	0.804LeuTrp: 0.804 ± 0.027	3.281LeuTyr: 3.281 ± 0.048	0.006LeuXaa: 0.006 ± 0.002
Met	1.852MetAla: 1.852 ± 0.038	0.177MetCys: 0.177 ± 0.011	1.259MetAsp: 1.259 ± 0.03	1.831MetGlu: 1.831 ± 0.038	1.151MetPhe: 1.151 ± 0.032	1.605MetGly: 1.605 ± 0.037	0.407MetHis: 0.407 ± 0.016	2.197MetIle: 2.197 ± 0.045	2.387MetLys: 2.387 ± 0.034	2.423MetLeu: 2.423 ± 0.044	0.853MetMet: 0.853 ± 0.025	1.473MetAsn: 1.473 ± 0.031	0.914MetPro: 0.914 ± 0.028	0.824MetGln: 0.824 ± 0.025	1.06MetArg: 1.06 ± 0.028	1.635MetSer: 1.635 ± 0.036	1.527MetThr: 1.527 ± 0.033	1.764MetVal: 1.764 ± 0.037	0.209MetTrp: 0.209 ± 0.014	0.73MetTyr: 0.73 ± 0.019	0.001MetXaa: 0.001 ± 0.001
Asn	2.502AsnAla: 2.502 ± 0.041	0.421AsnCys: 0.421 ± 0.018	2.441AsnAsp: 2.441 ± 0.047	4.084AsnGlu: 4.084 ± 0.053	1.968AsnPhe: 1.968 ± 0.04	3.283AsnGly: 3.283 ± 0.056	1.143AsnHis: 1.143 ± 0.03	3.571AsnIle: 3.571 ± 0.048	3.248AsnLys: 3.248 ± 0.046	4.28AsnLeu: 4.28 ± 0.058	1.268AsnMet: 1.268 ± 0.03	2.375AsnAsn: 2.375 ± 0.047	2.089AsnPro: 2.089 ± 0.039	1.95AsnGln: 1.95 ± 0.037	2.043AsnArg: 2.043 ± 0.041	2.738AsnSer: 2.738 ± 0.047	2.104AsnThr: 2.104 ± 0.041	3.219AsnVal: 3.219 ± 0.053	0.533AsnTrp: 0.533 ± 0.018	1.816AsnTyr: 1.816 ± 0.036	0.002AsnXaa: 0.002 ± 0.001
Pro	1.947ProAla: 1.947 ± 0.038	0.276ProCys: 0.276 ± 0.013	1.686ProAsp: 1.686 ± 0.042	2.645ProGlu: 2.645 ± 0.044	2.072ProPhe: 2.072 ± 0.039	2.072ProGly: 2.072 ± 0.043	0.761ProHis: 0.761 ± 0.024	3.097ProIle: 3.097 ± 0.051	2.266ProLys: 2.266 ± 0.034	3.485ProLeu: 3.485 ± 0.058	0.801ProMet: 0.801 ± 0.024	1.825ProAsn: 1.825 ± 0.036	1.031ProPro: 1.031 ± 0.033	1.032ProGln: 1.032 ± 0.031	1.236ProArg: 1.236 ± 0.033	2.12ProSer: 2.12 ± 0.032	2.118ProThr: 2.118 ± 0.041	2.456ProVal: 2.456 ± 0.043	0.349ProTrp: 0.349 ± 0.016	1.448ProTyr: 1.448 ± 0.035	0.003ProXaa: 0.003 ± 0.001
Gln	2.257GlnAla: 2.257 ± 0.05	0.258GlnCys: 0.258 ± 0.013	1.492GlnAsp: 1.492 ± 0.034	2.748GlnGlu: 2.748 ± 0.044	1.827GlnPhe: 1.827 ± 0.036	2.226GlnGly: 2.226 ± 0.042	0.78GlnHis: 0.78 ± 0.022	2.777GlnIle: 2.777 ± 0.04	2.773GlnLys: 2.773 ± 0.043	4.396GlnLeu: 4.396 ± 0.068	1.122GlnMet: 1.122 ± 0.028	1.53GlnAsn: 1.53 ± 0.032	1.194GlnPro: 1.194 ± 0.035	1.826GlnGln: 1.826 ± 0.049	1.549GlnArg: 1.549 ± 0.032	2.044GlnSer: 2.044 ± 0.036	1.818GlnThr: 1.818 ± 0.037	2.377GlnVal: 2.377 ± 0.046	0.419GlnTrp: 0.419 ± 0.019	1.324GlnTyr: 1.324 ± 0.032	0.001GlnXaa: 0.001 ± 0.001
Arg	2.246ArgAla: 2.246 ± 0.042	0.317ArgCys: 0.317 ± 0.017	2.032ArgAsp: 2.032 ± 0.039	3.177ArgGlu: 3.177 ± 0.056	1.95ArgPhe: 1.95 ± 0.033	2.452ArgGly: 2.452 ± 0.051	0.793ArgHis: 0.793 ± 0.029	3.02ArgIle: 3.02 ± 0.044	3.324ArgLys: 3.324 ± 0.051	4.072ArgLeu: 4.072 ± 0.06	1.17ArgMet: 1.17 ± 0.027	2.032ArgAsn: 2.032 ± 0.038	1.28ArgPro: 1.28 ± 0.037	1.525ArgGln: 1.525 ± 0.037	1.852ArgArg: 1.852 ± 0.037	2.377ArgSer: 2.377 ± 0.044	2.077ArgThr: 2.077 ± 0.036	2.652ArgVal: 2.652 ± 0.039	0.481ArgTrp: 0.481 ± 0.017	1.616ArgTyr: 1.616 ± 0.037	0.004ArgXaa: 0.004 ± 0.002
Ser	3.317SerAla: 3.317 ± 0.047	0.452SerCys: 0.452 ± 0.018	2.71SerAsp: 2.71 ± 0.04	4.05SerGlu: 4.05 ± 0.055	3.418SerPhe: 3.418 ± 0.056	3.987SerGly: 3.987 ± 0.059	1.161SerHis: 1.161 ± 0.029	5.254SerIle: 5.254 ± 0.069	4.067SerLys: 4.067 ± 0.06	6.261SerLeu: 6.261 ± 0.077	1.556SerMet: 1.556 ± 0.031	2.809SerAsn: 2.809 ± 0.043	1.996SerPro: 1.996 ± 0.04	2.038SerGln: 2.038 ± 0.038	2.388SerArg: 2.388 ± 0.042	3.931SerSer: 3.931 ± 0.058	3.162SerThr: 3.162 ± 0.048	4.01SerVal: 4.01 ± 0.052	0.629SerTrp: 0.629 ± 0.021	2.3SerTyr: 2.3 ± 0.038	0.009SerXaa: 0.009 ± 0.003
Thr	3.53ThrAla: 3.53 ± 0.053	0.427ThrCys: 0.427 ± 0.021	2.492ThrAsp: 2.492 ± 0.037	3.659ThrGlu: 3.659 ± 0.047	2.84ThrPhe: 2.84 ± 0.053	3.82ThrGly: 3.82 ± 0.057	1.046ThrHis: 1.046 ± 0.029	4.886ThrIle: 4.886 ± 0.059	3.749ThrLys: 3.749 ± 0.055	5.352ThrLeu: 5.352 ± 0.066	1.351ThrMet: 1.351 ± 0.034	2.701ThrAsn: 2.701 ± 0.047	2.248ThrPro: 2.248 ± 0.041	1.407ThrGln: 1.407 ± 0.033	1.887ThrArg: 1.887 ± 0.035	3.221ThrSer: 3.221 ± 0.048	3.173ThrThr: 3.173 ± 0.049	4.082ThrVal: 4.082 ± 0.064	0.529ThrTrp: 0.529 ± 0.02	1.922ThrTyr: 1.922 ± 0.035	0.007ThrXaa: 0.007 ± 0.002
Val	4.925ValAla: 4.925 ± 0.067	0.595ValCys: 0.595 ± 0.024	3.633ValAsp: 3.633 ± 0.051	5.167ValGlu: 5.167 ± 0.068	3.14ValPhe: 3.14 ± 0.052	4.654ValGly: 4.654 ± 0.067	1.325ValHis: 1.325 ± 0.03	5.948ValIle: 5.948 ± 0.063	4.609ValLys: 4.609 ± 0.057	6.598ValLeu: 6.598 ± 0.079	1.714ValMet: 1.714 ± 0.037	3.24ValAsn: 3.24 ± 0.052	2.606ValPro: 2.606 ± 0.039	2.283ValGln: 2.283 ± 0.045	2.618ValArg: 2.618 ± 0.041	4.58ValSer: 4.58 ± 0.058	4.235ValThr: 4.235 ± 0.057	5.268ValVal: 5.268 ± 0.07	0.605ValTrp: 0.605 ± 0.024	2.216ValTyr: 2.216 ± 0.038	0.005ValXaa: 0.005 ± 0.002
Trp	0.566TrpAla: 0.566 ± 0.022	0.083TrpCys: 0.083 ± 0.007	0.524TrpAsp: 0.524 ± 0.019	0.664TrpGlu: 0.664 ± 0.022	0.506TrpPhe: 0.506 ± 0.018	0.651TrpGly: 0.651 ± 0.024	0.197TrpHis: 0.197 ± 0.012	0.801TrpIle: 0.801 ± 0.024	0.715TrpLys: 0.715 ± 0.023	1.171TrpLeu: 1.171 ± 0.033	0.286TrpMet: 0.286 ± 0.015	0.549TrpAsn: 0.549 ± 0.019	0.235TrpPro: 0.235 ± 0.012	0.374TrpGln: 0.374 ± 0.016	0.424TrpArg: 0.424 ± 0.019	0.577TrpSer: 0.577 ± 0.02	0.478TrpThr: 0.478 ± 0.018	0.679TrpVal: 0.679 ± 0.023	0.14TrpTrp: 0.14 ± 0.011	0.382TrpTyr: 0.382 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.863TyrAla: 1.863 ± 0.035	0.397TyrCys: 0.397 ± 0.018	1.917TyrAsp: 1.917 ± 0.039	2.688TyrGlu: 2.688 ± 0.04	2.046TyrPhe: 2.046 ± 0.038	2.367TyrGly: 2.367 ± 0.046	0.927TyrHis: 0.927 ± 0.026	2.664TyrIle: 2.664 ± 0.046	2.123TyrLys: 2.123 ± 0.039	3.727TyrLeu: 3.727 ± 0.052	0.809TyrMet: 0.809 ± 0.024	1.506TyrAsn: 1.506 ± 0.03	1.358TyrPro: 1.358 ± 0.036	1.54TyrGln: 1.54 ± 0.032	1.64TyrArg: 1.64 ± 0.029	2.316TyrSer: 2.316 ± 0.04	1.731TyrThr: 1.731 ± 0.033	2.17TyrVal: 2.17 ± 0.037	0.386TyrTrp: 0.386 ± 0.018	1.509TyrTyr: 1.509 ± 0.034	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.003XaaAsp: 0.003 ± 0.002	0.004XaaGlu: 0.004 ± 0.002	0.008XaaPhe: 0.008 ± 0.003	0.002XaaGly: 0.002 ± 0.001	0.004XaaHis: 0.004 ± 0.002	0.003XaaIle: 0.003 ± 0.001	0.01XaaLys: 0.01 ± 0.003	0.005XaaLeu: 0.005 ± 0.002	0.003XaaMet: 0.003 ± 0.002	0.003XaaAsn: 0.003 ± 0.001	0.003XaaPro: 0.003 ± 0.002	0.003XaaGln: 0.003 ± 0.001	0.006XaaArg: 0.006 ± 0.002	0.006XaaSer: 0.006 ± 0.002	0.003XaaThr: 0.003 ± 0.001	0.004XaaVal: 0.004 ± 0.002	0.001XaaTrp: 0.001 ± 0.001	0.003XaaTyr: 0.003 ± 0.001	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 5454 proteins (1477624 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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