Natrinema versiforme icosahedral virus 1

Taxonomy: Viruses; Varidnaviria; Helvetiavirae; Dividoviricota; Laserviricetes; Halopanivirales; Simuloviridae; Yingchengvirus; Yingchengvirus NVIV1

Average proteome isoelectric point is 4.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G2JX66|A0A7G2JX66_9VIRU Sigma-70 family RNA polymerase sigma factor OS=Natrinema versiforme icosahedral virus 1 OX=2766529 GN=NVIV1gp16 PE=4 SV=1
MM1 pKa = 7.54QGGLSEE7 pKa = 4.2GTGPGEE13 pKa = 4.17LAVTATTTSSEE24 pKa = 4.26STATTSSPSNQSQQPPEE41 pKa = 4.37SPTAQLVYY49 pKa = 9.35GTQYY53 pKa = 9.46TQDD56 pKa = 4.35DD57 pKa = 4.59IIIGLLALQVVLLVFVTIRR76 pKa = 11.84SS77 pKa = 3.66

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G2JVR0|A0A7G2JVR0_9VIRU Large major capsid protein OS=Natrinema versiforme icosahedral virus 1 OX=2766529 GN=NVIV1gp06 PE=4 SV=1
MM1 pKa = 7.61AGYY4 pKa = 10.47AYY6 pKa = 9.53IEE8 pKa = 4.26SVVYY12 pKa = 10.3IRR14 pKa = 11.84EE15 pKa = 4.1EE16 pKa = 3.64PHH18 pKa = 7.0GVGLEE23 pKa = 3.64NRR25 pKa = 11.84FRR27 pKa = 11.84RR28 pKa = 11.84GWPTDD33 pKa = 3.31RR34 pKa = 11.84SITRR38 pKa = 11.84TMILSPVVACMDD50 pKa = 3.53WPSRR54 pKa = 11.84AAEE57 pKa = 4.01RR58 pKa = 11.84FAMAVGWIYY67 pKa = 11.38SDD69 pKa = 3.55GKK71 pKa = 10.42RR72 pKa = 11.84LAVVSLVLFALLIYY86 pKa = 10.45FNSLL90 pKa = 2.84

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

5310

66

954

204.2

22.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.527 ± 0.871

0.527 ± 0.119

10.791 ± 0.805

8.738 ± 0.645

2.618 ± 0.191

8.964 ± 0.856

1.243 ± 0.153

3.917 ± 0.466

2.015 ± 0.262

8.399 ± 0.846

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.601 ± 0.231

2.693 ± 0.499

4.765 ± 0.513

3.164 ± 0.53

6.177 ± 0.834

6.855 ± 0.648

6.347 ± 0.642

6.516 ± 0.369

1.789 ± 0.151

2.354 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski