Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Gluconacetobacter; Gluconacetobacter diazotrophicus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9H7G9|A9H7G9_GLUDA Uncharacterized protein OS=Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5) OX=272568 GN=GDI3695 PE=4 SV=1
MM1 pKa = 7.37SGSAARR7 pKa = 11.84FVWPNSFSVDD17 pKa = 3.11AAGVPRR23 pKa = 11.84AGARR27 pKa = 11.84LFFYY31 pKa = 9.65QTGTTTPQATYY42 pKa = 10.78ADD44 pKa = 4.06AGLTVPNANPVIADD58 pKa = 3.53EE59 pKa = 4.43NGQFGSIFLLGAPSYY74 pKa = 11.13AVALEE79 pKa = 4.5DD80 pKa = 5.08GEE82 pKa = 5.02GNPVWTMDD90 pKa = 3.7PVGAAGGGVGAVPVGTVADD109 pKa = 4.08YY110 pKa = 11.58AGATAPAGG118 pKa = 3.56

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A9HQC7|A9HQC7_GLUDA Putative alkyl hydroperoxide reductase OS=Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5) OX=272568 GN=ahpC PE=4 SV=1
MM1 pKa = 6.55TTPPRR6 pKa = 11.84RR7 pKa = 11.84PCSSPRR13 pKa = 11.84SWQRR17 pKa = 11.84HH18 pKa = 4.06RR19 pKa = 11.84QPGPFRR25 pKa = 11.84RR26 pKa = 11.84CRR28 pKa = 11.84PPHH31 pKa = 5.61FRR33 pKa = 11.84HH34 pKa = 6.44RR35 pKa = 11.84PLPPLQRR42 pKa = 11.84CKK44 pKa = 10.53PSWQHH49 pKa = 5.71PRR51 pKa = 11.84RR52 pKa = 11.84LPLRR56 pKa = 11.84VRR58 pKa = 11.84RR59 pKa = 11.84LPSRR63 pKa = 11.84QVRR66 pKa = 11.84QPRR69 pKa = 11.84PTPGQRR75 pKa = 11.84GQPRR79 pKa = 11.84QRR81 pKa = 11.84LPSHH85 pKa = 6.86RR86 pKa = 11.84PPFQSRR92 pKa = 11.84PPPRR96 pKa = 11.84RR97 pKa = 11.84QAAAHH102 pKa = 6.2RR103 pKa = 11.84KK104 pKa = 6.63SARR107 pKa = 11.84WRR109 pKa = 11.84HH110 pKa = 5.67CRR112 pKa = 11.84HH113 pKa = 4.84TWRR116 pKa = 11.84AQSSRR121 pKa = 11.84GRR123 pKa = 11.84RR124 pKa = 11.84APPRR128 pKa = 11.84APGRR132 pKa = 11.84SLAGYY137 pKa = 9.27RR138 pKa = 11.84RR139 pKa = 11.84KK140 pKa = 7.8TWW142 pKa = 2.86

Molecular weight: 17.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3783

0

3783

1180201

19

4453

312.0

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.989 ± 0.069

0.999 ± 0.013

5.879 ± 0.035

4.55 ± 0.039

3.332 ± 0.026

8.963 ± 0.042

2.4 ± 0.019

4.732 ± 0.029

2.108 ± 0.032

10.191 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.018

2.255 ± 0.025

5.902 ± 0.037

3.181 ± 0.028

8.256 ± 0.046

5.181 ± 0.034

5.564 ± 0.033

7.424 ± 0.034

1.413 ± 0.017

2.098 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski