Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Acetobacterium; Acetobacterium woodii

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6LDJ6|H6LDJ6_ACEWD NmrA domain-containing protein OS=Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) OX=931626 GN=Awo_c11840 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.31EE3 pKa = 3.82KK4 pKa = 10.71LMAMSNWFVKK14 pKa = 8.15TTVKK18 pKa = 10.57QKK20 pKa = 9.01ITLGVCTVAVFSMVGLGIYY39 pKa = 8.75QLIPNQSDD47 pKa = 3.6GLNLDD52 pKa = 3.89GTNKK56 pKa = 9.86TNVTVNEE63 pKa = 4.04INEE66 pKa = 4.06AQAAEE71 pKa = 4.12AEE73 pKa = 4.43KK74 pKa = 10.64LANTAAEE81 pKa = 4.28TTPVEE86 pKa = 5.0SVSQTQNEE94 pKa = 4.28ATNVVSNTTTVNTPTTTEE112 pKa = 3.89DD113 pKa = 3.79VYY115 pKa = 11.13IADD118 pKa = 4.95DD119 pKa = 4.09GAHH122 pKa = 5.91YY123 pKa = 10.2EE124 pKa = 4.15SEE126 pKa = 4.29PVYY129 pKa = 10.8SEE131 pKa = 3.83PAYY134 pKa = 9.92EE135 pKa = 4.02EE136 pKa = 4.08PVYY139 pKa = 10.46EE140 pKa = 4.39EE141 pKa = 4.18PAAASDD147 pKa = 3.96GGWDD151 pKa = 3.78GDD153 pKa = 4.16LGGDD157 pKa = 4.92GASEE161 pKa = 3.97WQLSDD166 pKa = 4.19DD167 pKa = 3.71AGEE170 pKa = 4.25YY171 pKa = 8.62GTGISNN177 pKa = 4.05

Molecular weight: 18.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P50002|ATPB_ACEWD ATP synthase subunit beta sodium ion specific OS=Acetobacterium woodii (strain ATCC 29683 / DSM 1030 / JCM 2381 / KCTC 1655 / WB1) OX=931626 GN=atpD PE=1 SV=3
MM1 pKa = 7.61LNTHH5 pKa = 6.28FRR7 pKa = 11.84NTKK10 pKa = 9.58LLCNVLQGLRR20 pKa = 11.84NRR22 pKa = 11.84YY23 pKa = 8.5FFASILSVRR32 pKa = 11.84AINAGMATGTSTKK45 pKa = 9.95ATINNEE51 pKa = 3.98RR52 pKa = 11.84IGYY55 pKa = 7.74LTEE58 pKa = 3.77TATAFPQNN66 pKa = 3.49

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3445

0

3445

1138432

22

2729

330.5

36.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.218 ± 0.043

1.251 ± 0.018

5.587 ± 0.028

6.949 ± 0.043

4.347 ± 0.032

6.811 ± 0.037

1.68 ± 0.019

8.725 ± 0.039

7.123 ± 0.038

9.391 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.021

5.169 ± 0.03

3.319 ± 0.023

3.462 ± 0.023

3.673 ± 0.025

5.753 ± 0.037

5.547 ± 0.04

6.604 ± 0.033

0.803 ± 0.013

3.67 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski