Botryotinia fuckeliana (strain T4) (Noble rot fungus) (Botrytis cinerea)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botrytis; Botrytis cinerea

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2XQZ6|G2XQZ6_BOTF4 Uncharacterized protein OS=Botryotinia fuckeliana (strain T4) OX=999810 GN=BofuT4P7000001001 PE=4 SV=1
MM1 pKa = 7.62CNWICTTEE9 pKa = 3.96VCPTCDD15 pKa = 2.76SKK17 pKa = 11.58QRR19 pKa = 11.84ISYY22 pKa = 10.69EE23 pKa = 4.01DD24 pKa = 3.17FHH26 pKa = 8.86CEE28 pKa = 4.06SNCYY32 pKa = 9.19PVRR35 pKa = 11.84QNAVHH40 pKa = 6.37CKK42 pKa = 10.14NYY44 pKa = 10.42RR45 pKa = 11.84KK46 pKa = 10.29LNNEE50 pKa = 4.27IIADD54 pKa = 4.11YY55 pKa = 10.15PCINCDD61 pKa = 3.32EE62 pKa = 5.47PISIFDD68 pKa = 4.79DD69 pKa = 3.72SDD71 pKa = 3.92SSEE74 pKa = 4.98DD75 pKa = 3.76GQKK78 pKa = 9.29TEE80 pKa = 4.34MDD82 pKa = 3.59KK83 pKa = 11.46LMNAIGTAMEE93 pKa = 4.47NKK95 pKa = 10.45SIDD98 pKa = 3.8EE99 pKa = 4.2ASKK102 pKa = 9.49TVAIDD107 pKa = 3.83DD108 pKa = 3.83VSEE111 pKa = 4.43DD112 pKa = 4.11EE113 pKa = 5.33RR114 pKa = 11.84DD115 pKa = 3.59SDD117 pKa = 4.44DD118 pKa = 4.21GNISEE123 pKa = 4.35VSEE126 pKa = 4.18VSAFSDD132 pKa = 3.55EE133 pKa = 3.86EE134 pKa = 5.44DD135 pKa = 3.99YY136 pKa = 11.79DD137 pKa = 4.34HH138 pKa = 6.38EE139 pKa = 4.4TAMEE143 pKa = 4.14GLMAGQIQYY152 pKa = 11.43DD153 pKa = 3.9DD154 pKa = 5.1DD155 pKa = 5.94DD156 pKa = 5.41SFDD159 pKa = 3.48VQEE162 pKa = 4.96FFGEE166 pKa = 3.85PDD168 pKa = 3.79LIEE171 pKa = 4.47EE172 pKa = 4.26EE173 pKa = 4.7SPAEE177 pKa = 3.56EE178 pKa = 4.46SYY180 pKa = 11.65YY181 pKa = 9.99LTSPRR186 pKa = 11.84LTRR189 pKa = 11.84TEE191 pKa = 3.73MLRR194 pKa = 11.84RR195 pKa = 11.84GWDD198 pKa = 2.96

Molecular weight: 22.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2XUQ7|G2XUQ7_BOTF4 Similar to WD repeat-containing protein OS=Botryotinia fuckeliana (strain T4) OX=999810 GN=BofuT4_P058070.1 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.33QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.46TRR48 pKa = 11.84IGLL51 pKa = 3.73

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16338

0

16338

5777822

33

6826

353.6

39.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.639 ± 0.02

1.258 ± 0.008

5.464 ± 0.016

6.456 ± 0.025

3.758 ± 0.015

6.677 ± 0.018

2.332 ± 0.009

5.519 ± 0.016

5.438 ± 0.02

8.57 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.009

4.26 ± 0.014

5.747 ± 0.021

3.902 ± 0.015

5.712 ± 0.021

8.865 ± 0.027

6.14 ± 0.019

5.733 ± 0.014

1.4 ± 0.008

2.831 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski