Candidatus Xiphinematobacter sp. Idaho Grape

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Spartobacteria; Candidatus Xiphinematobacter; unclassified Candidatus Xiphinematobacter

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0G9Y7|A0A0P0G9Y7_9BACT Cell division protein FtsL OS=Candidatus Xiphinematobacter sp. Idaho Grape OX=1704307 GN=ftsL PE=4 SV=1
MM1 pKa = 7.79ADD3 pKa = 3.47FNNRR7 pKa = 11.84YY8 pKa = 8.53PEE10 pKa = 4.19NVSGRR15 pKa = 11.84FYY17 pKa = 11.49VDD19 pKa = 4.08DD20 pKa = 3.67QCIDD24 pKa = 3.59CDD26 pKa = 4.06LCRR29 pKa = 11.84EE30 pKa = 4.03TAPANFKK37 pKa = 11.09RR38 pKa = 11.84NDD40 pKa = 3.32DD41 pKa = 3.67EE42 pKa = 4.79GRR44 pKa = 11.84SFVYY48 pKa = 9.96KK49 pKa = 10.36QPATIEE55 pKa = 4.12EE56 pKa = 4.45VEE58 pKa = 4.38LCQQALEE65 pKa = 4.42GCPVEE70 pKa = 5.41AIGDD74 pKa = 3.92SGIQEE79 pKa = 4.05

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0FIX8|A0A0P0FIX8_9BACT Lipoyl synthase OS=Candidatus Xiphinematobacter sp. Idaho Grape OX=1704307 GN=lipA PE=3 SV=1
MM1 pKa = 6.93VWKK4 pKa = 8.72KK5 pKa = 6.91TCRR8 pKa = 11.84RR9 pKa = 11.84TGLAKK14 pKa = 9.98TLGGLASSPRR24 pKa = 11.84ARR26 pKa = 11.84RR27 pKa = 11.84VRR29 pKa = 11.84GEE31 pKa = 3.56TDD33 pKa = 3.1GYY35 pKa = 10.61GGYY38 pKa = 10.2FISLGKK44 pKa = 10.48VCGTEE49 pKa = 3.52IGSLVIAFPRR59 pKa = 3.64

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

817

0

817

254565

29

1376

311.6

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.362 ± 0.073

1.576 ± 0.029

4.113 ± 0.057

6.051 ± 0.079

4.291 ± 0.05

7.441 ± 0.073

2.522 ± 0.038

6.434 ± 0.062

4.508 ± 0.059

11.04 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.03

3.025 ± 0.042

4.642 ± 0.047

3.75 ± 0.049

6.925 ± 0.062

6.966 ± 0.052

5.186 ± 0.048

7.318 ± 0.065

1.141 ± 0.035

2.6 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski