Amino acid dipepetide frequency for Paenibacillus sp. GP183

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.788AlaAla: 7.788 ± 0.097	0.713AlaCys: 0.713 ± 0.021	3.876AlaAsp: 3.876 ± 0.055	4.709AlaGlu: 4.709 ± 0.07	3.269AlaPhe: 3.269 ± 0.05	5.901AlaGly: 5.901 ± 0.073	1.414AlaHis: 1.414 ± 0.029	5.56AlaIle: 5.56 ± 0.054	4.524AlaLys: 4.524 ± 0.061	7.756AlaLeu: 7.756 ± 0.073	2.205AlaMet: 2.205 ± 0.034	2.755AlaAsn: 2.755 ± 0.052	2.6AlaPro: 2.6 ± 0.043	2.66AlaGln: 2.66 ± 0.041	3.236AlaArg: 3.236 ± 0.052	4.888AlaSer: 4.888 ± 0.067	3.434AlaThr: 3.434 ± 0.046	5.911AlaVal: 5.911 ± 0.069	0.853AlaTrp: 0.853 ± 0.023	2.547AlaTyr: 2.547 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.491CysAla: 0.491 ± 0.017	0.119CysCys: 0.119 ± 0.009	0.382CysAsp: 0.382 ± 0.016	0.469CysGlu: 0.469 ± 0.016	0.328CysPhe: 0.328 ± 0.015	0.772CysGly: 0.772 ± 0.025	0.217CysHis: 0.217 ± 0.01	0.568CysIle: 0.568 ± 0.018	0.418CysLys: 0.418 ± 0.015	0.818CysLeu: 0.818 ± 0.025	0.222CysMet: 0.222 ± 0.012	0.283CysAsn: 0.283 ± 0.013	0.395CysPro: 0.395 ± 0.017	0.233CysGln: 0.233 ± 0.013	0.418CysArg: 0.418 ± 0.018	0.635CysSer: 0.635 ± 0.019	0.435CysThr: 0.435 ± 0.014	0.468CysVal: 0.468 ± 0.017	0.102CysTrp: 0.102 ± 0.007	0.246CysTyr: 0.246 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.677AspAla: 3.677 ± 0.059	0.415AspCys: 0.415 ± 0.015	2.283AspAsp: 2.283 ± 0.046	3.482AspGlu: 3.482 ± 0.047	2.237AspPhe: 2.237 ± 0.039	3.492AspGly: 3.492 ± 0.057	1.222AspHis: 1.222 ± 0.025	3.638AspIle: 3.638 ± 0.054	2.94AspLys: 2.94 ± 0.045	5.216AspLeu: 5.216 ± 0.059	1.355AspMet: 1.355 ± 0.028	1.835AspAsn: 1.835 ± 0.036	2.452AspPro: 2.452 ± 0.043	2.121AspGln: 2.121 ± 0.036	2.512AspArg: 2.512 ± 0.038	2.877AspSer: 2.877 ± 0.046	2.385AspThr: 2.385 ± 0.042	3.369AspVal: 3.369 ± 0.042	0.779AspTrp: 0.779 ± 0.023	1.944AspTyr: 1.944 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.392GluAla: 5.392 ± 0.054	0.364GluCys: 0.364 ± 0.014	2.899GluAsp: 2.899 ± 0.042	4.648GluGlu: 4.648 ± 0.069	2.155GluPhe: 2.155 ± 0.039	3.809GluGly: 3.809 ± 0.054	1.529GluHis: 1.529 ± 0.034	4.628GluIle: 4.628 ± 0.062	4.297GluLys: 4.297 ± 0.061	6.783GluLeu: 6.783 ± 0.07	1.966GluMet: 1.966 ± 0.035	2.581GluAsn: 2.581 ± 0.042	2.058GluPro: 2.058 ± 0.034	3.391GluGln: 3.391 ± 0.056	3.44GluArg: 3.44 ± 0.053	3.535GluSer: 3.535 ± 0.046	3.098GluThr: 3.098 ± 0.046	4.237GluVal: 4.237 ± 0.063	0.858GluTrp: 0.858 ± 0.029	1.867GluTyr: 1.867 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	3.173PheAla: 3.173 ± 0.051	0.375PheCys: 0.375 ± 0.016	2.279PheAsp: 2.279 ± 0.034	2.495PheGlu: 2.495 ± 0.042	1.976PhePhe: 1.976 ± 0.043	3.344PheGly: 3.344 ± 0.049	1.021PheHis: 1.021 ± 0.025	3.309PheIle: 3.309 ± 0.05	2.191PheLys: 2.191 ± 0.042	4.259PheLeu: 4.259 ± 0.063	1.177PheMet: 1.177 ± 0.03	1.781PheAsn: 1.781 ± 0.035	1.736PhePro: 1.736 ± 0.033	1.608PheGln: 1.608 ± 0.031	1.915PheArg: 1.915 ± 0.036	3.039PheSer: 3.039 ± 0.051	2.521PheThr: 2.521 ± 0.04	2.934PheVal: 2.934 ± 0.048	0.523PheTrp: 0.523 ± 0.02	1.509PheTyr: 1.509 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.042GlyAla: 5.042 ± 0.07	0.721GlyCys: 0.721 ± 0.021	3.247GlyAsp: 3.247 ± 0.045	3.866GlyGlu: 3.866 ± 0.053	3.505GlyPhe: 3.505 ± 0.057	5.051GlyGly: 5.051 ± 0.072	1.488GlyHis: 1.488 ± 0.034	5.889GlyIle: 5.889 ± 0.065	4.714GlyLys: 4.714 ± 0.056	7.001GlyLeu: 7.001 ± 0.081	2.212GlyMet: 2.212 ± 0.036	2.761GlyAsn: 2.761 ± 0.046	1.868GlyPro: 1.868 ± 0.043	2.59GlyGln: 2.59 ± 0.055	3.108GlyArg: 3.108 ± 0.048	4.708GlySer: 4.708 ± 0.072	4.216GlyThr: 4.216 ± 0.061	4.91GlyVal: 4.91 ± 0.058	1.018GlyTrp: 1.018 ± 0.025	2.842GlyTyr: 2.842 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.528HisAla: 1.528 ± 0.033	0.208HisCys: 0.208 ± 0.01	1.033HisAsp: 1.033 ± 0.025	1.303HisGlu: 1.303 ± 0.031	1.096HisPhe: 1.096 ± 0.028	1.522HisGly: 1.522 ± 0.035	0.648HisHis: 0.648 ± 0.023	1.585HisIle: 1.585 ± 0.035	1.02HisLys: 1.02 ± 0.025	2.317HisLeu: 2.317 ± 0.045	0.612HisMet: 0.612 ± 0.02	0.78HisAsn: 0.78 ± 0.021	1.261HisPro: 1.261 ± 0.034	0.866HisGln: 0.866 ± 0.022	1.116HisArg: 1.116 ± 0.027	1.312HisSer: 1.312 ± 0.031	1.126HisThr: 1.126 ± 0.03	1.503HisVal: 1.503 ± 0.035	0.322HisTrp: 0.322 ± 0.017	0.895HisTyr: 0.895 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.913IleAla: 5.913 ± 0.072	0.708IleCys: 0.708 ± 0.022	3.922IleAsp: 3.922 ± 0.048	4.565IleGlu: 4.565 ± 0.06	2.843IlePhe: 2.843 ± 0.058	5.64IleGly: 5.64 ± 0.071	1.725IleHis: 1.725 ± 0.033	5.036IleIle: 5.036 ± 0.072	3.678IleLys: 3.678 ± 0.054	6.815IleLeu: 6.815 ± 0.081	1.897IleMet: 1.897 ± 0.04	2.883IleAsn: 2.883 ± 0.049	3.464IlePro: 3.464 ± 0.05	2.916IleGln: 2.916 ± 0.042	3.82IleArg: 3.82 ± 0.05	5.097IleSer: 5.097 ± 0.054	4.156IleThr: 4.156 ± 0.054	5.416IleVal: 5.416 ± 0.065	0.755IleTrp: 0.755 ± 0.025	2.252IleTyr: 2.252 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.603LysAla: 4.603 ± 0.062	0.289LysCys: 0.289 ± 0.014	3.235LysAsp: 3.235 ± 0.048	4.525LysGlu: 4.525 ± 0.055	1.853LysPhe: 1.853 ± 0.037	3.887LysGly: 3.887 ± 0.059	1.342LysHis: 1.342 ± 0.032	3.821LysIle: 3.821 ± 0.058	4.036LysLys: 4.036 ± 0.067	6.046LysLeu: 6.046 ± 0.075	1.843LysMet: 1.843 ± 0.036	2.61LysAsn: 2.61 ± 0.046	2.516LysPro: 2.516 ± 0.044	2.89LysGln: 2.89 ± 0.049	2.897LysArg: 2.897 ± 0.048	3.497LysSer: 3.497 ± 0.053	3.079LysThr: 3.079 ± 0.038	4.028LysVal: 4.028 ± 0.059	0.795LysTrp: 0.795 ± 0.026	1.906LysTyr: 1.906 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	7.847LeuAla: 7.847 ± 0.091	0.842LeuCys: 0.842 ± 0.024	5.106LeuAsp: 5.106 ± 0.059	6.234LeuGlu: 6.234 ± 0.066	4.661LeuPhe: 4.661 ± 0.079	6.887LeuGly: 6.887 ± 0.083	2.143LeuHis: 2.143 ± 0.036	7.361LeuIle: 7.361 ± 0.076	5.991LeuLys: 5.991 ± 0.067	10.946LeuLeu: 10.946 ± 0.135	2.682LeuMet: 2.682 ± 0.045	4.295LeuAsn: 4.295 ± 0.062	4.344LeuPro: 4.344 ± 0.058	4.349LeuGln: 4.349 ± 0.06	4.738LeuArg: 4.738 ± 0.059	7.27LeuSer: 7.27 ± 0.072	5.553LeuThr: 5.553 ± 0.057	6.358LeuVal: 6.358 ± 0.079	1.048LeuTrp: 1.048 ± 0.027	3.049LeuTyr: 3.049 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.134MetAla: 2.134 ± 0.041	0.153MetCys: 0.153 ± 0.009	1.615MetAsp: 1.615 ± 0.029	1.898MetGlu: 1.898 ± 0.039	1.085MetPhe: 1.085 ± 0.029	1.843MetGly: 1.843 ± 0.034	0.567MetHis: 0.567 ± 0.016	2.126MetIle: 2.126 ± 0.036	2.25MetLys: 2.25 ± 0.038	2.92MetLeu: 2.92 ± 0.048	0.866MetMet: 0.866 ± 0.026	1.593MetAsn: 1.593 ± 0.032	1.116MetPro: 1.116 ± 0.028	1.089MetGln: 1.089 ± 0.029	1.342MetArg: 1.342 ± 0.028	1.803MetSer: 1.803 ± 0.034	1.59MetThr: 1.59 ± 0.034	1.844MetVal: 1.844 ± 0.034	0.239MetTrp: 0.239 ± 0.011	0.72MetTyr: 0.72 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	2.817AsnAla: 2.817 ± 0.047	0.299AsnCys: 0.299 ± 0.015	1.992AsnAsp: 1.992 ± 0.035	2.682AsnGlu: 2.682 ± 0.041	1.585AsnPhe: 1.585 ± 0.034	3.155AsnGly: 3.155 ± 0.055	0.992AsnHis: 0.992 ± 0.027	2.728AsnIle: 2.728 ± 0.044	2.548AsnLys: 2.548 ± 0.044	3.82AsnLeu: 3.82 ± 0.055	1.197AsnMet: 1.197 ± 0.025	1.866AsnAsn: 1.866 ± 0.039	2.297AsnPro: 2.297 ± 0.041	1.839AsnGln: 1.839 ± 0.034	2.158AsnArg: 2.158 ± 0.038	2.427AsnSer: 2.427 ± 0.048	2.11AsnThr: 2.11 ± 0.039	2.813AsnVal: 2.813 ± 0.048	0.603AsnTrp: 0.603 ± 0.018	1.408AsnTyr: 1.408 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	3.119ProAla: 3.119 ± 0.054	0.235ProCys: 0.235 ± 0.012	2.46ProAsp: 2.46 ± 0.045	3.036ProGlu: 3.036 ± 0.045	2.024ProPhe: 2.024 ± 0.037	2.805ProGly: 2.805 ± 0.049	0.912ProHis: 0.912 ± 0.024	2.855ProIle: 2.855 ± 0.039	2.099ProLys: 2.099 ± 0.037	3.865ProLeu: 3.865 ± 0.055	1.043ProMet: 1.043 ± 0.027	1.753ProAsn: 1.753 ± 0.035	1.251ProPro: 1.251 ± 0.03	1.507ProGln: 1.507 ± 0.03	1.428ProArg: 1.428 ± 0.032	2.715ProSer: 2.715 ± 0.048	2.184ProThr: 2.184 ± 0.05	3.013ProVal: 3.013 ± 0.04	0.502ProTrp: 0.502 ± 0.019	1.53ProTyr: 1.53 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	3.347GlnAla: 3.347 ± 0.054	0.242GlnCys: 0.242 ± 0.012	1.81GlnAsp: 1.81 ± 0.035	2.558GlnGlu: 2.558 ± 0.042	1.648GlnPhe: 1.648 ± 0.032	2.608GlnGly: 2.608 ± 0.044	0.866GlnHis: 0.866 ± 0.023	3.108GlnIle: 3.108 ± 0.042	2.37GlnLys: 2.37 ± 0.04	4.322GlnLeu: 4.322 ± 0.063	1.294GlnMet: 1.294 ± 0.029	1.615GlnAsn: 1.615 ± 0.034	1.512GlnPro: 1.512 ± 0.032	1.993GlnGln: 1.993 ± 0.037	1.873GlnArg: 1.873 ± 0.034	2.414GlnSer: 2.414 ± 0.04	2.191GlnThr: 2.191 ± 0.041	2.736GlnVal: 2.736 ± 0.047	0.512GlnTrp: 0.512 ± 0.017	1.322GlnTyr: 1.322 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.903ArgAla: 2.903 ± 0.044	0.386ArgCys: 0.386 ± 0.019	2.337ArgAsp: 2.337 ± 0.039	3.265ArgGlu: 3.265 ± 0.047	2.248ArgPhe: 2.248 ± 0.04	2.91ArgGly: 2.91 ± 0.047	1.002ArgHis: 1.002 ± 0.028	3.818ArgIle: 3.818 ± 0.051	3.152ArgLys: 3.152 ± 0.045	4.909ArgLeu: 4.909 ± 0.062	1.61ArgMet: 1.61 ± 0.031	2.086ArgAsn: 2.086 ± 0.038	1.607ArgPro: 1.607 ± 0.033	1.859ArgGln: 1.859 ± 0.033	2.355ArgArg: 2.355 ± 0.047	2.901ArgSer: 2.901 ± 0.045	2.394ArgThr: 2.394 ± 0.038	2.954ArgVal: 2.954 ± 0.043	0.607ArgTrp: 0.607 ± 0.018	1.739ArgTyr: 1.739 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	4.583SerAla: 4.583 ± 0.062	0.465SerCys: 0.465 ± 0.019	3.079SerAsp: 3.079 ± 0.043	3.728SerGlu: 3.728 ± 0.053	3.181SerPhe: 3.181 ± 0.046	5.133SerGly: 5.133 ± 0.06	1.319SerHis: 1.319 ± 0.029	4.954SerIle: 4.954 ± 0.057	3.801SerLys: 3.801 ± 0.061	6.691SerLeu: 6.691 ± 0.075	2.002SerMet: 2.002 ± 0.036	2.62SerAsn: 2.62 ± 0.042	2.638SerPro: 2.638 ± 0.053	2.246SerGln: 2.246 ± 0.038	3.044SerArg: 3.044 ± 0.048	4.69SerSer: 4.69 ± 0.076	3.425SerThr: 3.425 ± 0.056	4.281SerVal: 4.281 ± 0.054	0.828SerTrp: 0.828 ± 0.023	2.273SerTyr: 2.273 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	4.399ThrAla: 4.399 ± 0.062	0.395ThrCys: 0.395 ± 0.014	2.71ThrAsp: 2.71 ± 0.047	3.044ThrGlu: 3.044 ± 0.044	2.36ThrPhe: 2.36 ± 0.041	4.309ThrGly: 4.309 ± 0.063	1.077ThrHis: 1.077 ± 0.023	4.038ThrIle: 4.038 ± 0.052	2.758ThrLys: 2.758 ± 0.045	5.429ThrLeu: 5.429 ± 0.055	1.405ThrMet: 1.405 ± 0.033	2.189ThrAsn: 2.189 ± 0.041	2.505ThrPro: 2.505 ± 0.048	1.703ThrGln: 1.703 ± 0.034	2.181ThrArg: 2.181 ± 0.032	3.394ThrSer: 3.394 ± 0.045	2.815ThrThr: 2.815 ± 0.052	4.046ThrVal: 4.046 ± 0.059	0.646ThrTrp: 0.646 ± 0.022	1.796ThrTyr: 1.796 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	4.762ValAla: 4.762 ± 0.061	0.652ValCys: 0.652 ± 0.025	3.544ValAsp: 3.544 ± 0.055	4.201ValGlu: 4.201 ± 0.062	2.859ValPhe: 2.859 ± 0.041	4.405ValGly: 4.405 ± 0.057	1.525ValHis: 1.525 ± 0.034	5.309ValIle: 5.309 ± 0.059	4.283ValLys: 4.283 ± 0.061	6.915ValLeu: 6.915 ± 0.079	1.904ValMet: 1.904 ± 0.033	3.019ValAsn: 3.019 ± 0.047	2.861ValPro: 2.861 ± 0.042	2.651ValGln: 2.651 ± 0.042	3.064ValArg: 3.064 ± 0.043	4.757ValSer: 4.757 ± 0.053	4.033ValThr: 4.033 ± 0.051	4.762ValVal: 4.762 ± 0.06	0.783ValTrp: 0.783 ± 0.021	2.251ValTyr: 2.251 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.715TrpAla: 0.715 ± 0.023	0.111TrpCys: 0.111 ± 0.009	0.62TrpAsp: 0.62 ± 0.019	0.732TrpGlu: 0.732 ± 0.02	0.603TrpPhe: 0.603 ± 0.02	0.839TrpGly: 0.839 ± 0.023	0.256TrpHis: 0.256 ± 0.011	0.979TrpIle: 0.979 ± 0.025	0.829TrpLys: 0.829 ± 0.024	1.42TrpLeu: 1.42 ± 0.038	0.414TrpMet: 0.414 ± 0.018	0.677TrpAsn: 0.677 ± 0.02	0.325TrpPro: 0.325 ± 0.017	0.493TrpGln: 0.493 ± 0.018	0.569TrpArg: 0.569 ± 0.02	0.82TrpSer: 0.82 ± 0.03	0.644TrpThr: 0.644 ± 0.022	0.785TrpVal: 0.785 ± 0.024	0.169TrpTrp: 0.169 ± 0.01	0.398TrpTyr: 0.398 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.365TyrAla: 2.365 ± 0.036	0.327TyrCys: 0.327 ± 0.015	1.804TyrAsp: 1.804 ± 0.037	2.096TyrGlu: 2.096 ± 0.038	1.653TyrPhe: 1.653 ± 0.03	2.445TyrGly: 2.445 ± 0.042	0.787TyrHis: 0.787 ± 0.023	2.189TyrIle: 2.189 ± 0.034	1.797TyrLys: 1.797 ± 0.039	3.457TyrLeu: 3.457 ± 0.051	0.884TyrMet: 0.884 ± 0.021	1.354TyrAsn: 1.354 ± 0.031	1.53TyrPro: 1.53 ± 0.03	1.338TyrGln: 1.338 ± 0.03	1.827TyrArg: 1.827 ± 0.036	2.2TyrSer: 2.2 ± 0.044	1.814TyrThr: 1.814 ± 0.034	2.17TyrVal: 2.17 ± 0.038	0.457TyrTrp: 0.457 ± 0.016	1.272TyrTyr: 1.272 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5493 proteins (1600958 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---