Mirabilis mosaic virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Caulimovirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8JTA5|Q8JTA5_9VIRU Aphid transmission protein OS=Mirabilis mosaic virus OX=194445 PE=3 SV=1
MM1 pKa = 6.98STISEE6 pKa = 4.2LAVLIEE12 pKa = 4.01EE13 pKa = 4.56LKK15 pKa = 11.04KK16 pKa = 10.72EE17 pKa = 4.18VADD20 pKa = 4.38LVTTVKK26 pKa = 10.72DD27 pKa = 3.45LAKK30 pKa = 10.57DD31 pKa = 3.47KK32 pKa = 11.44SPEE35 pKa = 3.83TDD37 pKa = 3.12QNALKK42 pKa = 10.85SLGAKK47 pKa = 9.6IIKK50 pKa = 9.87DD51 pKa = 3.2ISDD54 pKa = 4.75KK55 pKa = 10.61IDD57 pKa = 3.11QCEE60 pKa = 4.23CTDD63 pKa = 4.04KK64 pKa = 11.44LFDD67 pKa = 3.81RR68 pKa = 11.84LKK70 pKa = 11.0GKK72 pKa = 10.76GSVIPTQQEE81 pKa = 4.01TPEE84 pKa = 3.95VSEE87 pKa = 3.9GAKK90 pKa = 7.88YY91 pKa = 10.27QKK93 pKa = 10.75YY94 pKa = 9.65SWPNHH99 pKa = 4.82NVGNPEE105 pKa = 4.33LGSSGVKK112 pKa = 10.5NPVYY116 pKa = 9.94WPPTSFPSS124 pKa = 3.36

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8JTA6|Q8JTA6_9VIRU Uncharacterized protein OS=Mirabilis mosaic virus OX=194445 PE=3 SV=1
MM1 pKa = 7.61SNAQANPHH9 pKa = 6.57IYY11 pKa = 10.48KK12 pKa = 9.55KK13 pKa = 10.65DD14 pKa = 2.99KK15 pKa = 10.46YY16 pKa = 10.99VRR18 pKa = 11.84LKK20 pKa = 10.4PLEE23 pKa = 4.23PTSSKK28 pKa = 9.5KK29 pKa = 9.92RR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 9.33IFASNDD38 pKa = 3.13EE39 pKa = 4.24TSSIKK44 pKa = 9.98TIAAHH49 pKa = 6.23CNNLNTIVAKK59 pKa = 10.68NWLKK63 pKa = 10.7LSKK66 pKa = 10.36LLSYY70 pKa = 10.86LGLEE74 pKa = 4.2KK75 pKa = 10.87DD76 pKa = 3.29EE77 pKa = 4.72SEE79 pKa = 4.7AYY81 pKa = 10.3SKK83 pKa = 11.5NKK85 pKa = 9.81VPFGNFFTKK94 pKa = 10.42LRR96 pKa = 11.84ASKK99 pKa = 10.67VGEE102 pKa = 4.38DD103 pKa = 3.61KK104 pKa = 11.18EE105 pKa = 4.36KK106 pKa = 10.02TANLLPTLQEE116 pKa = 3.67MSSRR120 pKa = 11.84IGKK123 pKa = 9.75ISEE126 pKa = 4.16EE127 pKa = 4.21TKK129 pKa = 10.61KK130 pKa = 10.67LAEE133 pKa = 4.27NDD135 pKa = 3.51VVTNKK140 pKa = 9.71QLEE143 pKa = 4.57TIIKK147 pKa = 10.42DD148 pKa = 3.31FDD150 pKa = 3.69RR151 pKa = 11.84RR152 pKa = 11.84LSEE155 pKa = 3.7IQNSVKK161 pKa = 10.7AIVGG165 pKa = 3.57

Molecular weight: 18.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2384

75

674

340.6

39.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.446 ± 0.57

1.468 ± 0.324

5.453 ± 0.212

8.305 ± 0.741

4.111 ± 0.402

4.404 ± 0.422

2.307 ± 0.327

7.928 ± 0.391

10.57 ± 0.519

8.221 ± 0.554

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.971 ± 0.189

5.831 ± 0.581

4.698 ± 0.511

4.237 ± 0.238

4.153 ± 0.425

8.012 ± 0.825

4.992 ± 0.238

4.782 ± 0.47

1.133 ± 0.18

2.978 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski