Arthrobacter phage Gordon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; Arthrobacter virus Gordon

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4JL02|A0A0U4JL02_9CAUD Uncharacterized protein OS=Arthrobacter phage Gordon OX=1772298 GN=34 PE=4 SV=1
MM1 pKa = 7.04TTLTWDD7 pKa = 3.44VVGEE11 pKa = 4.16RR12 pKa = 11.84FYY14 pKa = 11.34EE15 pKa = 4.37AGTDD19 pKa = 3.41RR20 pKa = 11.84GVLYY24 pKa = 10.86LDD26 pKa = 4.16GVGHH30 pKa = 7.2AWNGLVEE37 pKa = 4.53VEE39 pKa = 4.13QSPSGGEE46 pKa = 3.82PEE48 pKa = 4.84TYY50 pKa = 10.31YY51 pKa = 10.85QDD53 pKa = 3.46GLPYY57 pKa = 10.17VSASAAEE64 pKa = 4.1EE65 pKa = 3.92FKK67 pKa = 11.29GSIEE71 pKa = 4.41AYY73 pKa = 8.28TYY75 pKa = 10.46PNAFAACDD83 pKa = 3.91GSQEE87 pKa = 4.1IAQGLYY93 pKa = 9.94ISQQDD98 pKa = 3.15RR99 pKa = 11.84KK100 pKa = 10.5QFGLTYY106 pKa = 9.3RR107 pKa = 11.84TLIGNDD113 pKa = 3.11INEE116 pKa = 4.58LDD118 pKa = 3.81HH119 pKa = 6.86GYY121 pKa = 10.28KK122 pKa = 9.63IHH124 pKa = 7.48ILYY127 pKa = 10.54NCMASPTTRR136 pKa = 11.84SYY138 pKa = 10.97STMGDD143 pKa = 3.27STDD146 pKa = 3.32ASTFNWKK153 pKa = 8.17ITTRR157 pKa = 11.84PVKK160 pKa = 10.71FEE162 pKa = 4.75DD163 pKa = 3.67PSFGIKK169 pKa = 10.18YY170 pKa = 9.46GAHH173 pKa = 5.95LTLDD177 pKa = 3.52SRR179 pKa = 11.84EE180 pKa = 4.11VYY182 pKa = 8.57PWAMAAVEE190 pKa = 3.98AVLYY194 pKa = 8.69GTEE197 pKa = 4.11TTEE200 pKa = 3.98PRR202 pKa = 11.84LPTPQEE208 pKa = 3.92LLDD211 pKa = 4.24LFVDD215 pKa = 3.7NALLKK220 pKa = 9.83ITDD223 pKa = 4.09NGDD226 pKa = 3.61GSWTAEE232 pKa = 4.19GPDD235 pKa = 4.41SIISMLTDD243 pKa = 4.75DD244 pKa = 5.09MFQIDD249 pKa = 3.99WPSAVVLDD257 pKa = 4.44SNTIQLSSLL266 pKa = 3.51

Molecular weight: 29.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4JZ99|A0A0U4JZ99_9CAUD Capsid & capsid maturation protease OS=Arthrobacter phage Gordon OX=1772298 GN=13 PE=4 SV=1
MM1 pKa = 7.91DD2 pKa = 5.07PVVLSTLIAGGTGCVISWALVTAFYY27 pKa = 10.67IFVQSRR33 pKa = 11.84GKK35 pKa = 9.6QDD37 pKa = 3.22KK38 pKa = 9.62SKK40 pKa = 10.12QWRR43 pKa = 11.84VDD45 pKa = 3.31PLAFEE50 pKa = 4.64PRR52 pKa = 11.84FGSPVSNWYY61 pKa = 7.96RR62 pKa = 11.84TWAWRR67 pKa = 11.84PVHH70 pKa = 5.69TVDD73 pKa = 5.58RR74 pKa = 11.84GYY76 pKa = 10.47IWLRR80 pKa = 11.84PYY82 pKa = 9.35WRR84 pKa = 11.84RR85 pKa = 11.84NIQRR89 pKa = 11.84HH90 pKa = 5.08AFLDD94 pKa = 3.99GGGPTFWWQNTVQLKK109 pKa = 10.66YY110 pKa = 10.75LDD112 pKa = 3.58QQ113 pKa = 5.8

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

18234

35

1530

204.9

22.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.583 ± 0.444

0.521 ± 0.084

5.994 ± 0.303

6.323 ± 0.298

3.691 ± 0.166

7.656 ± 0.416

1.887 ± 0.169

6.082 ± 0.27

6.247 ± 0.392

7.886 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.192

4.749 ± 0.2

4.184 ± 0.256

3.493 ± 0.127

5.221 ± 0.306

6.115 ± 0.176

6.389 ± 0.242

7.151 ± 0.173

1.579 ± 0.149

3.428 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski