Wenling thamnaconus septentrionalis filovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Thamnovirus; Huangjiao thamnovirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GMP6|A0A2P1GMP6_9MONO Uncharacterized protein OS=Wenling thamnaconus septentrionalis filovirus OX=2116488 PE=4 SV=1
MM1 pKa = 6.84STSEE5 pKa = 4.4KK6 pKa = 10.64EE7 pKa = 3.71MANRR11 pKa = 11.84IMKK14 pKa = 10.59DD15 pKa = 2.43SDD17 pKa = 3.63LQVIGLLLDD26 pKa = 4.03GLDD29 pKa = 3.94DD30 pKa = 3.57QGLDD34 pKa = 3.51VRR36 pKa = 11.84ALPDD40 pKa = 3.3PTGNRR45 pKa = 11.84DD46 pKa = 3.47YY47 pKa = 11.41TDD49 pKa = 2.75VSTFKK54 pKa = 11.04DD55 pKa = 4.4LFPMMSTSEE64 pKa = 4.57LIGSLSASGYY74 pKa = 10.14SDD76 pKa = 3.4QLEE79 pKa = 4.12KK80 pKa = 10.91SIEE83 pKa = 4.09STGRR87 pKa = 11.84KK88 pKa = 9.04LADD91 pKa = 3.79LARR94 pKa = 11.84SAPADD99 pKa = 3.81DD100 pKa = 4.54NAVQFMKK107 pKa = 10.34SYY109 pKa = 11.48LLMCEE114 pKa = 3.99KK115 pKa = 9.96TGQEE119 pKa = 4.26HH120 pKa = 6.6SVSLPKK126 pKa = 10.45EE127 pKa = 3.78EE128 pKa = 4.54RR129 pKa = 11.84KK130 pKa = 10.4GEE132 pKa = 4.23KK133 pKa = 8.83KK134 pKa = 9.12TGEE137 pKa = 4.05HH138 pKa = 6.05MKK140 pKa = 10.28PEE142 pKa = 4.31QSQGPQPTDD151 pKa = 3.23KK152 pKa = 11.25NKK154 pKa = 8.95TAAKK158 pKa = 9.79AGPSEE163 pKa = 4.04TGKK166 pKa = 10.85ANEE169 pKa = 4.2EE170 pKa = 4.04EE171 pKa = 4.66VTNKK175 pKa = 9.21TPAPRR180 pKa = 11.84RR181 pKa = 11.84NTPKK185 pKa = 10.4ALSGAEE191 pKa = 4.31TNDD194 pKa = 3.82SPLSTQLTQTPEE206 pKa = 3.69EE207 pKa = 4.32AGDD210 pKa = 3.76MFSGKK215 pKa = 10.45AEE217 pKa = 4.6GIVSGEE223 pKa = 3.63LRR225 pKa = 11.84TGKK228 pKa = 10.22KK229 pKa = 10.24LPTIVEE235 pKa = 4.26GCDD238 pKa = 2.92ASGRR242 pKa = 11.84GDD244 pKa = 3.32VTHH247 pKa = 7.68LYY249 pKa = 10.08RR250 pKa = 11.84SHH252 pKa = 6.66EE253 pKa = 4.11LVEE256 pKa = 4.91RR257 pKa = 11.84IQKK260 pKa = 10.12GEE262 pKa = 3.75KK263 pKa = 9.57TMGIACLKK271 pKa = 9.27ALVMGEE277 pKa = 4.52DD278 pKa = 3.67NFSGVPFDD286 pKa = 6.75DD287 pKa = 4.45ILDD290 pKa = 4.29CFGEE294 pKa = 4.46TCDD297 pKa = 6.07DD298 pKa = 4.52PDD300 pKa = 3.93TPGLRR305 pKa = 11.84GLMEE309 pKa = 4.93GSNGPASGLPCTDD322 pKa = 4.96DD323 pKa = 3.39YY324 pKa = 11.63CGPSTVLFDD333 pKa = 3.72RR334 pKa = 11.84WAKK337 pKa = 10.5AGSLGCVNYY346 pKa = 9.8MIRR349 pKa = 11.84RR350 pKa = 11.84CMGDD354 pKa = 3.1LYY356 pKa = 11.28DD357 pKa = 4.28GLRR360 pKa = 11.84AAACDD365 pKa = 3.59TPAYY369 pKa = 7.57YY370 pKa = 10.41HH371 pKa = 7.26EE372 pKa = 4.81MAQQQKK378 pKa = 10.6DD379 pKa = 3.66CTDD382 pKa = 3.22QVVKK386 pKa = 10.64VVSGMEE392 pKa = 3.98EE393 pKa = 3.61MGEE396 pKa = 3.86AMQEE400 pKa = 3.74LRR402 pKa = 11.84RR403 pKa = 11.84EE404 pKa = 4.02NTEE407 pKa = 3.55MKK409 pKa = 10.45RR410 pKa = 11.84RR411 pKa = 11.84IADD414 pKa = 3.11MAAAMEE420 pKa = 4.41HH421 pKa = 6.15LSGVVSSLSSKK432 pKa = 11.01LEE434 pKa = 3.88TAVVVSSGYY443 pKa = 9.65PSSKK447 pKa = 8.62EE448 pKa = 3.49TAAGIKK454 pKa = 10.17GSTYY458 pKa = 11.05NEE460 pKa = 4.31DD461 pKa = 3.22QQTWKK466 pKa = 9.32TGVSSWQKK474 pKa = 10.26RR475 pKa = 11.84EE476 pKa = 3.69TSIWTNPSEE485 pKa = 5.41RR486 pKa = 11.84SPQEE490 pKa = 3.89LNTHH494 pKa = 6.09GGIGEE499 pKa = 4.56DD500 pKa = 4.23DD501 pKa = 3.77SEE503 pKa = 5.59AFLKK507 pKa = 10.46QLEE510 pKa = 4.43EE511 pKa = 3.63QWAAEE516 pKa = 4.1SSDD519 pKa = 3.48PLSFGTSGRR528 pKa = 11.84GINDD532 pKa = 2.88QDD534 pKa = 3.94FVRR537 pKa = 11.84EE538 pKa = 4.23TLTGTPYY545 pKa = 10.75EE546 pKa = 4.29GDD548 pKa = 3.56YY549 pKa = 10.8NASRR553 pKa = 11.84EE554 pKa = 4.44SKK556 pKa = 10.2KK557 pKa = 10.87GPRR560 pKa = 11.84FF561 pKa = 3.43

Molecular weight: 60.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GMM5|A0A2P1GMM5_9MONO Nucleoprotein OS=Wenling thamnaconus septentrionalis filovirus OX=2116488 PE=4 SV=1
MM1 pKa = 6.89ITNLITTSLRR11 pKa = 11.84KK12 pKa = 9.56KK13 pKa = 9.78KK14 pKa = 10.29RR15 pKa = 11.84KK16 pKa = 8.58EE17 pKa = 3.61KK18 pKa = 10.75RR19 pKa = 11.84KK20 pKa = 7.92TKK22 pKa = 10.29PIGGNMASKK31 pKa = 9.89PVRR34 pKa = 11.84GGGSGGGSGHH44 pKa = 7.01RR45 pKa = 11.84PFHH48 pKa = 6.53PNQLPQYY55 pKa = 8.37KK56 pKa = 9.65ARR58 pKa = 11.84SRR60 pKa = 11.84ICYY63 pKa = 9.31GFLSGTCKK71 pKa = 10.3RR72 pKa = 11.84GISCRR77 pKa = 11.84YY78 pKa = 7.81QHH80 pKa = 6.85DD81 pKa = 4.36LKK83 pKa = 11.26CGGEE87 pKa = 4.04RR88 pKa = 11.84EE89 pKa = 4.03QLLFSRR95 pKa = 11.84MKK97 pKa = 10.51VDD99 pKa = 4.16QLLLANSKK107 pKa = 9.72GRR109 pKa = 11.84APQMVKK115 pKa = 10.93DD116 pKa = 4.0NLLSGVLNEE125 pKa = 4.08IKK127 pKa = 10.52KK128 pKa = 10.13RR129 pKa = 11.84VPKK132 pKa = 10.77SEE134 pKa = 4.04DD135 pKa = 3.07RR136 pKa = 11.84CTLGDD141 pKa = 3.9SDD143 pKa = 5.74LKK145 pKa = 10.56TMLNGFEE152 pKa = 4.43KK153 pKa = 11.1VNAGLNNPQRR163 pKa = 11.84LEE165 pKa = 3.82SWDD168 pKa = 3.48PSGTVDD174 pKa = 5.27LGRR177 pKa = 11.84LSLLGNPQRR186 pKa = 11.84LGWRR190 pKa = 11.84LGHH193 pKa = 7.2LKK195 pKa = 10.67ALTCAQLVILLDD207 pKa = 3.9FLVKK211 pKa = 10.59ALTDD215 pKa = 3.57MEE217 pKa = 4.6EE218 pKa = 4.18EE219 pKa = 4.42GSEE222 pKa = 4.64GEE224 pKa = 4.13GSLWGARR231 pKa = 11.84HH232 pKa = 5.1EE233 pKa = 4.37HH234 pKa = 6.25SSRR237 pKa = 11.84GCRR240 pKa = 11.84YY241 pKa = 9.11YY242 pKa = 11.01KK243 pKa = 10.51SGMCFKK249 pKa = 10.21MGRR252 pKa = 11.84CLYY255 pKa = 10.33SHH257 pKa = 6.92NPYY260 pKa = 10.8KK261 pKa = 10.83LGASDD266 pKa = 5.52LIRR269 pKa = 11.84GIAIGLQSLDD279 pKa = 4.37LGSQPLCEE287 pKa = 4.4DD288 pKa = 3.09SGMPSLPQDD297 pKa = 3.04RR298 pKa = 11.84KK299 pKa = 10.28ARR301 pKa = 11.84KK302 pKa = 9.69DD303 pKa = 3.15MARR306 pKa = 11.84ALISEE311 pKa = 4.41SAINPRR317 pKa = 11.84LPKK320 pKa = 10.44EE321 pKa = 4.26SLLTMAQLIWEE332 pKa = 4.36WDD334 pKa = 3.56YY335 pKa = 11.88VPPSEE340 pKa = 5.53SGPTPPSSGDD350 pKa = 3.23VPGEE354 pKa = 3.93VRR356 pKa = 11.84DD357 pKa = 3.77MLLTLKK363 pKa = 10.73ALEE366 pKa = 4.52GPKK369 pKa = 10.4GVAAALTRR377 pKa = 11.84YY378 pKa = 8.72SQEE381 pKa = 3.32HH382 pKa = 6.69RR383 pKa = 11.84GRR385 pKa = 11.84LLISMEE391 pKa = 3.99KK392 pKa = 10.6KK393 pKa = 8.95MM394 pKa = 4.99

Molecular weight: 43.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4352

221

2146

725.3

80.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.043 ± 0.899

2.022 ± 0.264

5.331 ± 0.651

5.767 ± 0.723

3.699 ± 0.454

7.835 ± 0.759

2.642 ± 0.604

4.848 ± 0.483

4.894 ± 0.96

11.213 ± 0.795

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.24 ± 0.504

3.608 ± 0.446

4.825 ± 0.299

3.378 ± 0.221

6.296 ± 0.349

7.813 ± 0.818

6.319 ± 0.511

6.664 ± 0.761

1.241 ± 0.082

2.321 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski