Serratia phage JS26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F3E9|A0A5Q2F3E9_9CAUD Uncharacterized protein OS=Serratia phage JS26 OX=2315217 PE=4 SV=1
MM1 pKa = 6.87PTGMRR6 pKa = 11.84ALRR9 pKa = 11.84AQALKK14 pKa = 10.71AAILNFMDD22 pKa = 5.3FADD25 pKa = 3.77QAIPEE30 pKa = 5.2DD31 pKa = 4.27DD32 pKa = 4.04CCMCGDD38 pKa = 3.91KK39 pKa = 10.51QDD41 pKa = 3.37NHH43 pKa = 6.48TFDD46 pKa = 3.73SGHH49 pKa = 7.02AFTAATQYY57 pKa = 11.17HH58 pKa = 6.99AFTLRR63 pKa = 11.84KK64 pKa = 9.23EE65 pKa = 4.1VEE67 pKa = 4.08KK68 pKa = 11.13YY69 pKa = 11.03LDD71 pKa = 3.47VLEE74 pKa = 4.46TDD76 pKa = 3.31EE77 pKa = 4.64ATTVTHH83 pKa = 7.16TDD85 pKa = 3.19LQGNEE90 pKa = 3.94SQVAVFGPSSFPMWGQGSCCNKK112 pKa = 9.67QAEE115 pKa = 4.69LYY117 pKa = 10.74ARR119 pKa = 11.84ATGADD124 pKa = 3.52VYY126 pKa = 11.65ADD128 pKa = 3.85GEE130 pKa = 4.69LIAGQHH136 pKa = 5.14VLWKK140 pKa = 9.85KK141 pKa = 10.58GEE143 pKa = 4.08PGIPEE148 pKa = 4.28QILDD152 pKa = 3.85SNGDD156 pKa = 3.61VVLACCKK163 pKa = 10.04ICGDD167 pKa = 4.09YY168 pKa = 10.99EE169 pKa = 4.56QGLLDD174 pKa = 4.9NPVCPGKK181 pKa = 10.39KK182 pKa = 10.19ASDD185 pKa = 3.53NGG187 pKa = 3.77

Molecular weight: 20.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F2R1|A0A5Q2F2R1_9CAUD Uncharacterized protein OS=Serratia phage JS26 OX=2315217 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TIIEE6 pKa = 4.12ATAVAEE12 pKa = 3.95LAKK15 pKa = 10.17TFEE18 pKa = 4.7RR19 pKa = 11.84YY20 pKa = 9.31PEE22 pKa = 3.9QSQQAIRR29 pKa = 11.84MAINDD34 pKa = 3.56VAKK37 pKa = 10.62GKK39 pKa = 10.18GMKK42 pKa = 10.54LIRR45 pKa = 11.84TSMMAEE51 pKa = 3.89VAFPGSYY58 pKa = 10.93LNGDD62 pKa = 3.76RR63 pKa = 11.84LQITQRR69 pKa = 11.84ATNSNPQAVITARR82 pKa = 11.84KK83 pKa = 9.07RR84 pKa = 11.84ATSLARR90 pKa = 11.84FASGTPSSTAKK101 pKa = 10.4KK102 pKa = 9.95GVTVRR107 pKa = 11.84VKK109 pKa = 10.38KK110 pKa = 10.73GKK112 pKa = 9.68SVHH115 pKa = 6.41LKK117 pKa = 10.47SGWLVRR123 pKa = 11.84LRR125 pKa = 11.84AGASMSEE132 pKa = 4.01DD133 pKa = 3.37NYY135 pKa = 11.32NIGLAVRR142 pKa = 11.84LGPGEE147 pKa = 4.03KK148 pKa = 9.94LANKK152 pKa = 7.3TTTHH156 pKa = 6.18TSWLVKK162 pKa = 10.54GSVALLYY169 pKa = 10.63GPSVNQVFSTVAEE182 pKa = 4.25DD183 pKa = 3.43VSRR186 pKa = 11.84PIGDD190 pKa = 3.51MVTTEE195 pKa = 4.61YY196 pKa = 10.36LRR198 pKa = 11.84QMARR202 pKa = 11.84LLEE205 pKa = 4.25

Molecular weight: 22.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

20039

47

1380

238.6

26.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.851 ± 0.623

1.023 ± 0.114

6.597 ± 0.258

6.093 ± 0.263

3.478 ± 0.143

7.321 ± 0.331

1.757 ± 0.129

5.28 ± 0.161

5.599 ± 0.264

7.715 ± 0.212

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.209 ± 0.144

3.932 ± 0.157

4.466 ± 0.319

4.137 ± 0.262

5.659 ± 0.246

5.38 ± 0.215

6.527 ± 0.251

6.837 ± 0.279

1.722 ± 0.126

3.418 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski