Klebsiella phage vB_KpnM_15-38_KLPPOU148

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Jedunavirus; unclassified Jedunavirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J0E3|A0A6B9J0E3_9CAUD HTH tetR-type domain-containing protein OS=Klebsiella phage vB_KpnM_15-38_KLPPOU148 OX=2686208 GN=KLPPOU148_025 PE=4 SV=1
MM1 pKa = 7.44TLLGQLITDD10 pKa = 3.63MVEE13 pKa = 3.84KK14 pKa = 10.87QNFEE18 pKa = 4.26NKK20 pKa = 9.75VNTLVNMPEE29 pKa = 3.89WEE31 pKa = 4.93FITVMGYY38 pKa = 10.82GFFDD42 pKa = 4.01EE43 pKa = 5.5LCAGTTYY50 pKa = 10.72KK51 pKa = 10.44GAYY54 pKa = 7.16YY55 pKa = 9.9TEE57 pKa = 4.0QEE59 pKa = 4.01IYY61 pKa = 9.62AAYY64 pKa = 8.97WKK66 pKa = 10.07RR67 pKa = 11.84NPDD70 pKa = 3.7AEE72 pKa = 4.32PLLL75 pKa = 4.45

Molecular weight: 8.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9JBH8|A0A6B9JBH8_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_15-38_KLPPOU148 OX=2686208 GN=KLPPOU148_040 PE=4 SV=1
MM1 pKa = 7.14IQILKK6 pKa = 8.69WFLWRR11 pKa = 11.84RR12 pKa = 11.84WKK14 pKa = 10.45LKK16 pKa = 9.32HH17 pKa = 5.01WRR19 pKa = 11.84KK20 pKa = 9.99EE21 pKa = 3.74GGFNDD26 pKa = 3.36VFLRR30 pKa = 11.84NCHH33 pKa = 5.33NCNLAGCRR41 pKa = 11.84KK42 pKa = 10.03HH43 pKa = 6.27PVIYY47 pKa = 10.65NDD49 pKa = 2.87MCRR52 pKa = 11.84IVRR55 pKa = 11.84GGKK58 pKa = 9.29

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

14372

43

752

199.6

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.377 ± 0.357

1.538 ± 0.14

6.255 ± 0.173

6.005 ± 0.277

3.514 ± 0.205

7.515 ± 0.335

2.011 ± 0.2

5.852 ± 0.162

5.483 ± 0.297

7.306 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.783 ± 0.149

4.731 ± 0.307

4.112 ± 0.238

4.008 ± 0.205

5.302 ± 0.259

6.033 ± 0.305

6.638 ± 0.353

7.048 ± 0.253

1.739 ± 0.105

3.75 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski