Amino acid dipepetide frequency for Streptomyces phage Sebastisaurus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.348AlaAla: 14.348 ± 1.241
0.825AlaCys: 0.825 ± 0.289
9.401AlaAsp: 9.401 ± 0.904
7.504AlaGlu: 7.504 ± 1.123
3.216AlaPhe: 3.216 ± 0.493
9.895AlaGly: 9.895 ± 0.868
1.319AlaHis: 1.319 ± 0.35
3.876AlaIle: 3.876 ± 0.617
5.277AlaLys: 5.277 ± 0.84
10.802AlaLeu: 10.802 ± 0.868
2.969AlaMet: 2.969 ± 0.559
2.969AlaAsn: 2.969 ± 0.485
6.514AlaPro: 6.514 ± 0.911
3.876AlaGln: 3.876 ± 0.573
7.669AlaArg: 7.669 ± 1.004
6.514AlaSer: 6.514 ± 0.828
6.927AlaThr: 6.927 ± 0.659
10.06AlaVal: 10.06 ± 0.921
1.979AlaTrp: 1.979 ± 0.297
3.134AlaTyr: 3.134 ± 0.532
0.0AlaXaa: 0.0 ± 0.0
Cys
1.237CysAla: 1.237 ± 0.44
0.0CysCys: 0.0 ± 0.0
0.742CysAsp: 0.742 ± 0.206
0.495CysGlu: 0.495 ± 0.223
0.082CysPhe: 0.082 ± 0.067
0.495CysGly: 0.495 ± 0.199
0.165CysHis: 0.165 ± 0.134
0.247CysIle: 0.247 ± 0.122
0.577CysLys: 0.577 ± 0.25
0.412CysLeu: 0.412 ± 0.233
0.082CysMet: 0.082 ± 0.075
0.33CysAsn: 0.33 ± 0.184
0.66CysPro: 0.66 ± 0.341
0.247CysGln: 0.247 ± 0.141
0.577CysArg: 0.577 ± 0.252
0.412CysSer: 0.412 ± 0.168
0.495CysThr: 0.495 ± 0.182
0.495CysVal: 0.495 ± 0.218
0.082CysTrp: 0.082 ± 0.096
0.165CysTyr: 0.165 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
8.741AspAla: 8.741 ± 0.888
0.0AspCys: 0.0 ± 0.0
4.948AspAsp: 4.948 ± 0.916
5.03AspGlu: 5.03 ± 0.82
2.804AspPhe: 2.804 ± 0.409
5.772AspGly: 5.772 ± 0.777
1.154AspHis: 1.154 ± 0.321
1.649AspIle: 1.649 ± 0.386
2.804AspLys: 2.804 ± 0.61
6.432AspLeu: 6.432 ± 0.629
1.319AspMet: 1.319 ± 0.328
1.567AspAsn: 1.567 ± 0.358
3.793AspPro: 3.793 ± 0.481
1.319AspGln: 1.319 ± 0.345
3.134AspArg: 3.134 ± 0.5
2.804AspSer: 2.804 ± 0.433
3.381AspThr: 3.381 ± 0.454
5.855AspVal: 5.855 ± 0.716
1.154AspTrp: 1.154 ± 0.244
1.484AspTyr: 1.484 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
6.679GluAla: 6.679 ± 0.891
0.33GluCys: 0.33 ± 0.17
3.381GluAsp: 3.381 ± 0.631
3.298GluGlu: 3.298 ± 0.47
3.298GluPhe: 3.298 ± 0.493
4.123GluGly: 4.123 ± 0.733
1.237GluHis: 1.237 ± 0.257
2.474GluIle: 2.474 ± 0.482
2.556GluLys: 2.556 ± 0.62
5.03GluLeu: 5.03 ± 0.64
0.742GluMet: 0.742 ± 0.241
1.402GluAsn: 1.402 ± 0.36
3.546GluPro: 3.546 ± 0.897
1.154GluGln: 1.154 ± 0.251
5.442GluArg: 5.442 ± 0.813
3.134GluSer: 3.134 ± 0.418
5.195GluThr: 5.195 ± 0.754
4.37GluVal: 4.37 ± 0.643
1.649GluTrp: 1.649 ± 0.339
1.649GluTyr: 1.649 ± 0.276
0.0GluXaa: 0.0 ± 0.0
Phe
3.134PheAla: 3.134 ± 0.442
0.165PheCys: 0.165 ± 0.118
1.814PheAsp: 1.814 ± 0.372
1.072PheGlu: 1.072 ± 0.19
0.742PhePhe: 0.742 ± 0.243
3.298PheGly: 3.298 ± 0.598
0.412PheHis: 0.412 ± 0.154
1.072PheIle: 1.072 ± 0.272
1.732PheLys: 1.732 ± 0.343
2.474PheLeu: 2.474 ± 0.462
0.907PheMet: 0.907 ± 0.236
1.319PheAsn: 1.319 ± 0.295
1.814PhePro: 1.814 ± 0.411
0.66PheGln: 0.66 ± 0.209
2.226PheArg: 2.226 ± 0.386
2.556PheSer: 2.556 ± 0.469
3.051PheThr: 3.051 ± 0.378
1.979PheVal: 1.979 ± 0.465
0.33PheTrp: 0.33 ± 0.153
0.577PheTyr: 0.577 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
9.401GlyAla: 9.401 ± 1.009
0.825GlyCys: 0.825 ± 0.239
5.277GlyAsp: 5.277 ± 0.676
4.535GlyGlu: 4.535 ± 0.604
3.134GlyPhe: 3.134 ± 0.37
5.855GlyGly: 5.855 ± 0.883
1.154GlyHis: 1.154 ± 0.238
3.134GlyIle: 3.134 ± 0.511
4.453GlyLys: 4.453 ± 0.588
7.669GlyLeu: 7.669 ± 1.163
1.897GlyMet: 1.897 ± 0.342
2.721GlyAsn: 2.721 ± 0.446
4.7GlyPro: 4.7 ± 0.698
2.721GlyGln: 2.721 ± 0.496
4.865GlyArg: 4.865 ± 0.5
5.03GlySer: 5.03 ± 0.639
5.113GlyThr: 5.113 ± 0.637
7.339GlyVal: 7.339 ± 1.027
1.319GlyTrp: 1.319 ± 0.398
2.391GlyTyr: 2.391 ± 0.579
0.0GlyXaa: 0.0 ± 0.0
His
1.567HisAla: 1.567 ± 0.309
0.247HisCys: 0.247 ± 0.119
1.237HisAsp: 1.237 ± 0.287
1.732HisGlu: 1.732 ± 0.423
0.495HisPhe: 0.495 ± 0.19
0.825HisGly: 0.825 ± 0.328
0.247HisHis: 0.247 ± 0.147
0.66HisIle: 0.66 ± 0.245
1.237HisLys: 1.237 ± 0.331
1.237HisLeu: 1.237 ± 0.275
0.412HisMet: 0.412 ± 0.215
0.33HisAsn: 0.33 ± 0.149
0.907HisPro: 0.907 ± 0.255
0.247HisGln: 0.247 ± 0.114
1.072HisArg: 1.072 ± 0.28
1.567HisSer: 1.567 ± 0.367
0.742HisThr: 0.742 ± 0.272
1.154HisVal: 1.154 ± 0.265
0.495HisTrp: 0.495 ± 0.197
0.165HisTyr: 0.165 ± 0.12
0.0HisXaa: 0.0 ± 0.0
Ile
4.453IleAla: 4.453 ± 0.639
0.247IleCys: 0.247 ± 0.15
2.721IleAsp: 2.721 ± 0.454
1.814IleGlu: 1.814 ± 0.36
0.577IlePhe: 0.577 ± 0.189
3.628IleGly: 3.628 ± 0.588
0.742IleHis: 0.742 ± 0.24
0.99IleIle: 0.99 ± 0.376
1.567IleLys: 1.567 ± 0.383
2.309IleLeu: 2.309 ± 0.423
0.577IleMet: 0.577 ± 0.213
1.237IleAsn: 1.237 ± 0.359
1.402IlePro: 1.402 ± 0.278
1.319IleGln: 1.319 ± 0.351
2.639IleArg: 2.639 ± 0.534
1.072IleSer: 1.072 ± 0.318
3.298IleThr: 3.298 ± 0.538
2.721IleVal: 2.721 ± 0.39
0.495IleTrp: 0.495 ± 0.183
0.825IleTyr: 0.825 ± 0.243
0.0IleXaa: 0.0 ± 0.0
Lys
5.69LysAla: 5.69 ± 0.908
0.165LysCys: 0.165 ± 0.088
2.639LysAsp: 2.639 ± 0.406
2.804LysGlu: 2.804 ± 0.509
0.495LysPhe: 0.495 ± 0.252
3.051LysGly: 3.051 ± 0.564
0.742LysHis: 0.742 ± 0.231
1.402LysIle: 1.402 ± 0.35
2.144LysLys: 2.144 ± 0.556
4.453LysLeu: 4.453 ± 0.67
0.495LysMet: 0.495 ± 0.23
1.567LysAsn: 1.567 ± 0.464
2.969LysPro: 2.969 ± 0.438
1.484LysGln: 1.484 ± 0.369
3.876LysArg: 3.876 ± 0.725
3.216LysSer: 3.216 ± 0.488
3.298LysThr: 3.298 ± 0.518
3.876LysVal: 3.876 ± 0.572
0.66LysTrp: 0.66 ± 0.241
1.319LysTyr: 1.319 ± 0.39
0.0LysXaa: 0.0 ± 0.0
Leu
8.988LeuAla: 8.988 ± 0.842
0.66LeuCys: 0.66 ± 0.19
5.855LeuAsp: 5.855 ± 0.878
4.205LeuGlu: 4.205 ± 0.785
2.144LeuPhe: 2.144 ± 0.406
4.865LeuGly: 4.865 ± 0.554
1.072LeuHis: 1.072 ± 0.343
3.793LeuIle: 3.793 ± 0.813
3.793LeuLys: 3.793 ± 0.599
5.277LeuLeu: 5.277 ± 0.668
1.897LeuMet: 1.897 ± 0.377
3.051LeuAsn: 3.051 ± 0.37
5.525LeuPro: 5.525 ± 0.653
1.402LeuGln: 1.402 ± 0.361
5.195LeuArg: 5.195 ± 0.802
5.277LeuSer: 5.277 ± 0.745
7.669LeuThr: 7.669 ± 0.978
6.432LeuVal: 6.432 ± 0.85
0.825LeuTrp: 0.825 ± 0.217
1.897LeuTyr: 1.897 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
2.804MetAla: 2.804 ± 0.439
0.412MetCys: 0.412 ± 0.186
0.742MetAsp: 0.742 ± 0.256
0.412MetGlu: 0.412 ± 0.194
0.577MetPhe: 0.577 ± 0.223
2.309MetGly: 2.309 ± 0.424
0.33MetHis: 0.33 ± 0.144
0.577MetIle: 0.577 ± 0.174
0.66MetLys: 0.66 ± 0.31
1.897MetLeu: 1.897 ± 0.36
0.247MetMet: 0.247 ± 0.147
0.577MetAsn: 0.577 ± 0.201
1.072MetPro: 1.072 ± 0.307
0.742MetGln: 0.742 ± 0.257
1.814MetArg: 1.814 ± 0.392
1.484MetSer: 1.484 ± 0.322
2.639MetThr: 2.639 ± 0.454
1.154MetVal: 1.154 ± 0.308
0.165MetTrp: 0.165 ± 0.098
0.412MetTyr: 0.412 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
5.03AsnAla: 5.03 ± 0.719
0.165AsnCys: 0.165 ± 0.124
1.732AsnAsp: 1.732 ± 0.4
2.062AsnGlu: 2.062 ± 0.345
1.237AsnPhe: 1.237 ± 0.32
3.958AsnGly: 3.958 ± 0.526
0.33AsnHis: 0.33 ± 0.13
1.154AsnIle: 1.154 ± 0.25
1.402AsnLys: 1.402 ± 0.344
1.732AsnLeu: 1.732 ± 0.324
0.99AsnMet: 0.99 ± 0.252
0.742AsnAsn: 0.742 ± 0.228
2.391AsnPro: 2.391 ± 0.396
0.412AsnGln: 0.412 ± 0.208
1.237AsnArg: 1.237 ± 0.273
1.319AsnSer: 1.319 ± 0.271
1.732AsnThr: 1.732 ± 0.379
2.309AsnVal: 2.309 ± 0.425
0.247AsnTrp: 0.247 ± 0.175
0.742AsnTyr: 0.742 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
7.421ProAla: 7.421 ± 0.978
0.577ProCys: 0.577 ± 0.246
4.041ProAsp: 4.041 ± 0.603
4.948ProGlu: 4.948 ± 0.828
1.732ProPhe: 1.732 ± 0.305
5.195ProGly: 5.195 ± 0.653
0.907ProHis: 0.907 ± 0.247
2.144ProIle: 2.144 ± 0.444
2.556ProLys: 2.556 ± 0.486
2.226ProLeu: 2.226 ± 0.423
1.319ProMet: 1.319 ± 0.317
2.721ProAsn: 2.721 ± 0.504
1.567ProPro: 1.567 ± 0.524
1.072ProGln: 1.072 ± 0.239
2.474ProArg: 2.474 ± 0.426
3.546ProSer: 3.546 ± 0.495
3.628ProThr: 3.628 ± 0.544
4.618ProVal: 4.618 ± 0.745
0.742ProTrp: 0.742 ± 0.224
1.402ProTyr: 1.402 ± 0.301
0.0ProXaa: 0.0 ± 0.0
Gln
3.134GlnAla: 3.134 ± 0.434
0.412GlnCys: 0.412 ± 0.161
1.237GlnAsp: 1.237 ± 0.269
1.732GlnGlu: 1.732 ± 0.357
0.99GlnPhe: 0.99 ± 0.21
2.474GlnGly: 2.474 ± 0.364
0.247GlnHis: 0.247 ± 0.127
0.66GlnIle: 0.66 ± 0.208
0.495GlnLys: 0.495 ± 0.167
1.897GlnLeu: 1.897 ± 0.482
0.577GlnMet: 0.577 ± 0.229
0.907GlnAsn: 0.907 ± 0.237
0.907GlnPro: 0.907 ± 0.319
0.742GlnGln: 0.742 ± 0.287
3.216GlnArg: 3.216 ± 0.431
1.237GlnSer: 1.237 ± 0.272
2.062GlnThr: 2.062 ± 0.459
2.309GlnVal: 2.309 ± 0.468
0.66GlnTrp: 0.66 ± 0.268
0.825GlnTyr: 0.825 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
7.092ArgAla: 7.092 ± 0.762
0.495ArgCys: 0.495 ± 0.253
4.288ArgAsp: 4.288 ± 0.53
5.113ArgGlu: 5.113 ± 0.836
2.886ArgPhe: 2.886 ± 0.434
5.36ArgGly: 5.36 ± 0.802
1.649ArgHis: 1.649 ± 0.496
2.474ArgIle: 2.474 ± 0.423
3.876ArgLys: 3.876 ± 0.563
5.607ArgLeu: 5.607 ± 0.679
1.732ArgMet: 1.732 ± 0.4
1.979ArgAsn: 1.979 ± 0.362
2.474ArgPro: 2.474 ± 0.474
1.979ArgGln: 1.979 ± 0.426
3.711ArgArg: 3.711 ± 0.504
3.381ArgSer: 3.381 ± 0.658
4.041ArgThr: 4.041 ± 0.571
4.7ArgVal: 4.7 ± 0.543
1.319ArgTrp: 1.319 ± 0.387
2.062ArgTyr: 2.062 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
6.679SerAla: 6.679 ± 0.792
0.742SerCys: 0.742 ± 0.239
3.546SerAsp: 3.546 ± 0.658
3.298SerGlu: 3.298 ± 0.576
1.237SerPhe: 1.237 ± 0.261
4.288SerGly: 4.288 ± 0.469
0.907SerHis: 0.907 ± 0.309
2.226SerIle: 2.226 ± 0.382
2.309SerLys: 2.309 ± 0.465
4.948SerLeu: 4.948 ± 0.451
0.99SerMet: 0.99 ± 0.286
1.484SerAsn: 1.484 ± 0.372
3.628SerPro: 3.628 ± 0.486
1.897SerGln: 1.897 ± 0.349
3.298SerArg: 3.298 ± 0.447
2.556SerSer: 2.556 ± 0.462
4.535SerThr: 4.535 ± 0.595
5.113SerVal: 5.113 ± 0.675
1.649SerTrp: 1.649 ± 0.341
1.649SerTyr: 1.649 ± 0.474
0.0SerXaa: 0.0 ± 0.0
Thr
8.329ThrAla: 8.329 ± 0.837
0.577ThrCys: 0.577 ± 0.186
3.958ThrAsp: 3.958 ± 0.651
3.793ThrGlu: 3.793 ± 0.609
2.474ThrPhe: 2.474 ± 0.354
8.246ThrGly: 8.246 ± 0.802
1.402ThrHis: 1.402 ± 0.349
1.979ThrIle: 1.979 ± 0.423
3.958ThrLys: 3.958 ± 0.536
5.69ThrLeu: 5.69 ± 0.584
0.825ThrMet: 0.825 ± 0.255
2.226ThrAsn: 2.226 ± 0.453
3.546ThrPro: 3.546 ± 0.655
1.814ThrGln: 1.814 ± 0.34
4.123ThrArg: 4.123 ± 0.672
4.37ThrSer: 4.37 ± 0.597
5.277ThrThr: 5.277 ± 0.729
6.514ThrVal: 6.514 ± 0.694
1.319ThrTrp: 1.319 ± 0.411
2.556ThrTyr: 2.556 ± 0.413
0.0ThrXaa: 0.0 ± 0.0
Val
9.401ValAla: 9.401 ± 1.042
0.742ValCys: 0.742 ± 0.225
5.113ValAsp: 5.113 ± 0.558
3.216ValGlu: 3.216 ± 0.476
2.144ValPhe: 2.144 ± 0.441
5.772ValGly: 5.772 ± 0.686
1.732ValHis: 1.732 ± 0.366
3.381ValIle: 3.381 ± 0.645
2.721ValLys: 2.721 ± 0.501
5.937ValLeu: 5.937 ± 0.676
2.309ValMet: 2.309 ± 0.49
2.804ValAsn: 2.804 ± 0.457
5.607ValPro: 5.607 ± 0.837
2.062ValGln: 2.062 ± 0.353
5.937ValArg: 5.937 ± 0.808
4.865ValSer: 4.865 ± 0.566
7.257ValThr: 7.257 ± 0.735
6.514ValVal: 6.514 ± 0.765
1.649ValTrp: 1.649 ± 0.311
2.474ValTyr: 2.474 ± 0.37
0.0ValXaa: 0.0 ± 0.0
Trp
1.237TrpAla: 1.237 ± 0.345
0.247TrpCys: 0.247 ± 0.128
0.825TrpAsp: 0.825 ± 0.26
1.319TrpGlu: 1.319 ± 0.289
0.742TrpPhe: 0.742 ± 0.302
1.237TrpGly: 1.237 ± 0.249
0.412TrpHis: 0.412 ± 0.167
0.412TrpIle: 0.412 ± 0.19
0.825TrpLys: 0.825 ± 0.255
2.062TrpLeu: 2.062 ± 0.371
0.0TrpMet: 0.0 ± 0.0
0.577TrpAsn: 0.577 ± 0.199
0.33TrpPro: 0.33 ± 0.16
0.907TrpGln: 0.907 ± 0.257
1.237TrpArg: 1.237 ± 0.36
0.99TrpSer: 0.99 ± 0.253
1.402TrpThr: 1.402 ± 0.274
1.814TrpVal: 1.814 ± 0.45
0.33TrpTrp: 0.33 ± 0.157
0.33TrpTyr: 0.33 ± 0.135
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.711TyrAla: 3.711 ± 0.52
0.165TyrCys: 0.165 ± 0.126
1.649TyrAsp: 1.649 ± 0.337
2.391TyrGlu: 2.391 ± 0.466
0.33TyrPhe: 0.33 ± 0.151
2.886TyrGly: 2.886 ± 0.465
0.412TyrHis: 0.412 ± 0.183
0.33TyrIle: 0.33 ± 0.16
1.402TyrLys: 1.402 ± 0.346
1.732TyrLeu: 1.732 ± 0.263
0.66TyrMet: 0.66 ± 0.24
0.412TyrAsn: 0.412 ± 0.148
1.484TyrPro: 1.484 ± 0.409
0.66TyrGln: 0.66 ± 0.265
2.474TyrArg: 2.474 ± 0.569
1.649TyrSer: 1.649 ± 0.319
1.237TyrThr: 1.237 ± 0.318
2.309TyrVal: 2.309 ± 0.319
0.165TyrTrp: 0.165 ± 0.097
0.247TyrTyr: 0.247 ± 0.139
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12128 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski