Vaprio virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ledantevirus; Vaprio ledantevirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1BSY6|A0A2P1BSY6_9RHAB Nucleocapsid protein OS=Vaprio virus OX=2100727 PE=4 SV=1
MM1 pKa = 6.94NRR3 pKa = 11.84SRR5 pKa = 11.84VKK7 pKa = 10.51EE8 pKa = 3.56LANRR12 pKa = 11.84QFFKK16 pKa = 10.74DD17 pKa = 3.34IEE19 pKa = 4.41GKK21 pKa = 10.05QFDD24 pKa = 4.17EE25 pKa = 6.16DD26 pKa = 4.15EE27 pKa = 5.69DD28 pKa = 3.9EE29 pKa = 4.72LTYY32 pKa = 10.83QDD34 pKa = 4.15VKK36 pKa = 10.46PTGSNLFEE44 pKa = 4.48RR45 pKa = 11.84KK46 pKa = 9.05EE47 pKa = 4.08DD48 pKa = 3.57EE49 pKa = 4.31LEE51 pKa = 3.88IRR53 pKa = 11.84PEE55 pKa = 3.74EE56 pKa = 4.11EE57 pKa = 3.76EE58 pKa = 3.82FDD60 pKa = 3.81EE61 pKa = 5.01EE62 pKa = 4.16EE63 pKa = 4.48SEE65 pKa = 4.38EE66 pKa = 4.0EE67 pKa = 4.37GEE69 pKa = 5.15GIEE72 pKa = 5.03EE73 pKa = 5.94DD74 pKa = 3.89LDD76 pKa = 3.51QDD78 pKa = 3.83QDD80 pKa = 4.29VEE82 pKa = 4.34FHH84 pKa = 7.29DD85 pKa = 4.99PPEE88 pKa = 4.35QVDD91 pKa = 3.94SEE93 pKa = 4.4NDD95 pKa = 3.44GMVSAVEE102 pKa = 4.01NLDD105 pKa = 3.43EE106 pKa = 4.38EE107 pKa = 4.63SRR109 pKa = 11.84EE110 pKa = 3.76EE111 pKa = 4.17SYY113 pKa = 10.92IEE115 pKa = 4.65SEE117 pKa = 4.33LNHH120 pKa = 6.61LALSDD125 pKa = 3.65HH126 pKa = 6.8LEE128 pKa = 4.32SKK130 pKa = 10.37MSASEE135 pKa = 4.03KK136 pKa = 10.12AASSEE141 pKa = 4.34DD142 pKa = 3.43EE143 pKa = 4.36SEE145 pKa = 3.98PDD147 pKa = 3.09EE148 pKa = 4.87VKK150 pKa = 10.52IEE152 pKa = 3.92FDD154 pKa = 4.25KK155 pKa = 11.25IQLPAVRR162 pKa = 11.84SQEE165 pKa = 4.1EE166 pKa = 3.97LDD168 pKa = 4.78DD169 pKa = 3.56ICFGMFHH176 pKa = 7.01ALVSKK181 pKa = 10.5IGWSLIPLHH190 pKa = 6.72CKK192 pKa = 10.35AGPKK196 pKa = 9.07TLTVSMAPVGKK207 pKa = 9.49PKK209 pKa = 10.25RR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84NSSYY216 pKa = 11.13NEE218 pKa = 3.77EE219 pKa = 4.09PPILEE224 pKa = 4.07NLKK227 pKa = 10.01EE228 pKa = 4.17VNKK231 pKa = 10.26GKK233 pKa = 10.44SSEE236 pKa = 4.3TIEE239 pKa = 4.09EE240 pKa = 4.2NKK242 pKa = 10.52DD243 pKa = 3.2GRR245 pKa = 11.84FKK247 pKa = 11.8DD248 pKa = 3.58MVSKK252 pKa = 10.61IRR254 pKa = 11.84AGQIRR259 pKa = 11.84FMKK262 pKa = 10.12KK263 pKa = 9.7KK264 pKa = 9.5NNGYY268 pKa = 8.4MVLGMDD274 pKa = 4.04TPGMSEE280 pKa = 4.73DD281 pKa = 4.45LIEE284 pKa = 4.6RR285 pKa = 11.84CLNNSANEE293 pKa = 4.19GEE295 pKa = 4.6AVEE298 pKa = 5.17KK299 pKa = 10.25MLEE302 pKa = 4.14EE303 pKa = 4.17TGMLPLVQALCKK315 pKa = 10.65YY316 pKa = 9.41PP317 pKa = 3.48

Molecular weight: 36.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1BSX2|A0A2P1BSX2_9RHAB Matrix OS=Vaprio virus OX=2100727 PE=4 SV=1
MM1 pKa = 7.15AQARR5 pKa = 11.84SEE7 pKa = 4.23SGQEE11 pKa = 3.53NQLRR15 pKa = 11.84KK16 pKa = 10.18GRR18 pKa = 11.84TIMSRR23 pKa = 11.84FKK25 pKa = 10.99SSFSSHH31 pKa = 5.8VDD33 pKa = 3.17SGPKK37 pKa = 9.93SVTVLIATSDD47 pKa = 3.58SDD49 pKa = 3.39IKK51 pKa = 10.78IAIGQDD57 pKa = 2.94KK58 pKa = 10.34KK59 pKa = 10.97INEE62 pKa = 4.84LIDD65 pKa = 3.4YY66 pKa = 10.27SVIGKK71 pKa = 8.81DD72 pKa = 3.27EE73 pKa = 4.05KK74 pKa = 10.15RR75 pKa = 11.84TKK77 pKa = 10.28KK78 pKa = 9.54IQRR81 pKa = 11.84ANMVWKK87 pKa = 10.46QNYY90 pKa = 6.23EE91 pKa = 3.99TEE93 pKa = 4.2KK94 pKa = 11.06VNNCLYY100 pKa = 10.11CWKK103 pKa = 10.34GSQGRR108 pKa = 11.84MKK110 pKa = 10.15KK111 pKa = 10.53VSFFEE116 pKa = 4.19SDD118 pKa = 2.94EE119 pKa = 4.55FIYY122 pKa = 10.24VQNEE126 pKa = 3.46QGMMDD131 pKa = 4.46KK132 pKa = 10.79YY133 pKa = 11.28VALNDD138 pKa = 4.01PKK140 pKa = 10.97NLQKK144 pKa = 10.95GKK146 pKa = 8.15EE147 pKa = 4.19VKK149 pKa = 10.32LHH151 pKa = 6.02GPIPEE156 pKa = 4.21YY157 pKa = 11.15

Molecular weight: 18.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3807

157

2127

634.5

72.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.571 ± 1.046

1.839 ± 0.197

6.12 ± 0.257

7.407 ± 1.359

4.597 ± 0.322

5.963 ± 0.33

2.679 ± 0.31

6.436 ± 0.556

7.985 ± 0.513

9.614 ± 1.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.278

4.833 ± 0.449

4.492 ± 0.35

2.758 ± 0.423

5.096 ± 0.341

7.434 ± 0.505

4.781 ± 0.443

5.779 ± 0.252

1.629 ± 0.227

3.283 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski