Amino acid dipepetide frequency for Lachnospiraceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.299AlaAla: 7.299 ± 0.135	0.931AlaCys: 0.931 ± 0.03	5.144AlaAsp: 5.144 ± 0.08	6.453AlaGlu: 6.453 ± 0.111	3.118AlaPhe: 3.118 ± 0.058	6.167AlaGly: 6.167 ± 0.084	1.174AlaHis: 1.174 ± 0.031	4.804AlaIle: 4.804 ± 0.099	4.408AlaLys: 4.408 ± 0.072	6.704AlaLeu: 6.704 ± 0.085	2.362AlaMet: 2.362 ± 0.087	2.444AlaAsn: 2.444 ± 0.053	2.159AlaPro: 2.159 ± 0.053	1.861AlaGln: 1.861 ± 0.046	2.854AlaArg: 2.854 ± 0.061	4.59AlaSer: 4.59 ± 0.078	2.98AlaThr: 2.98 ± 0.061	6.723AlaVal: 6.723 ± 0.091	0.629AlaTrp: 0.629 ± 0.023	2.583AlaTyr: 2.583 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.941CysAla: 0.941 ± 0.03	0.242CysCys: 0.242 ± 0.017	0.858CysAsp: 0.858 ± 0.028	0.81CysGlu: 0.81 ± 0.024	0.601CysPhe: 0.601 ± 0.026	1.358CysGly: 1.358 ± 0.036	0.27CysHis: 0.27 ± 0.017	0.989CysIle: 0.989 ± 0.029	0.698CysLys: 0.698 ± 0.023	1.014CysLeu: 1.014 ± 0.029	0.394CysMet: 0.394 ± 0.02	0.513CysAsn: 0.513 ± 0.022	0.567CysPro: 0.567 ± 0.025	0.276CysGln: 0.276 ± 0.014	0.638CysArg: 0.638 ± 0.027	0.848CysSer: 0.848 ± 0.026	0.708CysThr: 0.708 ± 0.025	0.9CysVal: 0.9 ± 0.027	0.108CysTrp: 0.108 ± 0.01	0.484CysTyr: 0.484 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	4.819AspAla: 4.819 ± 0.097	0.723AspCys: 0.723 ± 0.026	4.218AspAsp: 4.218 ± 0.104	5.436AspGlu: 5.436 ± 0.08	2.917AspPhe: 2.917 ± 0.057	4.953AspGly: 4.953 ± 0.083	1.251AspHis: 1.251 ± 0.037	5.083AspIle: 5.083 ± 0.069	3.922AspLys: 3.922 ± 0.064	4.964AspLeu: 4.964 ± 0.067	1.969AspMet: 1.969 ± 0.041	2.55AspAsn: 2.55 ± 0.053	2.396AspPro: 2.396 ± 0.057	1.492AspGln: 1.492 ± 0.041	3.053AspArg: 3.053 ± 0.063	3.766AspSer: 3.766 ± 0.063	3.394AspThr: 3.394 ± 0.058	4.157AspVal: 4.157 ± 0.068	0.599AspTrp: 0.599 ± 0.023	2.894AspTyr: 2.894 ± 0.06	0.0AspXaa: 0.0 ± 0.0
Glu	5.738GluAla: 5.738 ± 0.105	0.769GluCys: 0.769 ± 0.026	4.517GluAsp: 4.517 ± 0.063	6.382GluGlu: 6.382 ± 0.107	2.678GluPhe: 2.678 ± 0.051	4.281GluGly: 4.281 ± 0.056	1.305GluHis: 1.305 ± 0.041	5.838GluIle: 5.838 ± 0.09	7.012GluLys: 7.012 ± 0.087	6.433GluLeu: 6.433 ± 0.088	2.36GluMet: 2.36 ± 0.054	4.261GluAsn: 4.261 ± 0.065	1.909GluPro: 1.909 ± 0.063	2.125GluGln: 2.125 ± 0.042	3.354GluArg: 3.354 ± 0.06	3.873GluSer: 3.873 ± 0.071	3.777GluThr: 3.777 ± 0.062	4.435GluVal: 4.435 ± 0.064	0.681GluTrp: 0.681 ± 0.025	3.149GluTyr: 3.149 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	3.003PheAla: 3.003 ± 0.055	0.687PheCys: 0.687 ± 0.027	2.936PheAsp: 2.936 ± 0.054	2.777PheGlu: 2.777 ± 0.051	2.101PhePhe: 2.101 ± 0.054	3.178PheGly: 3.178 ± 0.059	0.795PheHis: 0.795 ± 0.025	3.243PheIle: 3.243 ± 0.064	2.159PheLys: 2.159 ± 0.046	3.839PheLeu: 3.839 ± 0.068	1.312PheMet: 1.312 ± 0.031	1.784PheAsn: 1.784 ± 0.041	1.324PhePro: 1.324 ± 0.037	1.015PheGln: 1.015 ± 0.031	2.085PheArg: 2.085 ± 0.051	3.042PheSer: 3.042 ± 0.059	2.596PheThr: 2.596 ± 0.05	2.879PheVal: 2.879 ± 0.055	0.437PheTrp: 0.437 ± 0.022	1.81PheTyr: 1.81 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.995GlyAla: 4.995 ± 0.088	1.126GlyCys: 1.126 ± 0.037	4.09GlyAsp: 4.09 ± 0.064	4.719GlyGlu: 4.719 ± 0.066	3.356GlyPhe: 3.356 ± 0.052	4.656GlyGly: 4.656 ± 0.081	1.321GlyHis: 1.321 ± 0.034	5.888GlyIle: 5.888 ± 0.086	5.456GlyLys: 5.456 ± 0.082	5.581GlyLeu: 5.581 ± 0.078	2.336GlyMet: 2.336 ± 0.05	3.294GlyAsn: 3.294 ± 0.066	1.362GlyPro: 1.362 ± 0.038	1.787GlyGln: 1.787 ± 0.044	3.151GlyArg: 3.151 ± 0.064	4.416GlySer: 4.416 ± 0.067	4.264GlyThr: 4.264 ± 0.076	5.001GlyVal: 5.001 ± 0.076	0.69GlyTrp: 0.69 ± 0.029	3.28GlyTyr: 3.28 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.16HisAla: 1.16 ± 0.033	0.255HisCys: 0.255 ± 0.017	1.133HisAsp: 1.133 ± 0.041	1.243HisGlu: 1.243 ± 0.033	0.86HisPhe: 0.86 ± 0.03	1.312HisGly: 1.312 ± 0.034	0.415HisHis: 0.415 ± 0.025	1.386HisIle: 1.386 ± 0.034	0.935HisLys: 0.935 ± 0.029	1.324HisLeu: 1.324 ± 0.039	0.568HisMet: 0.568 ± 0.023	0.717HisAsn: 0.717 ± 0.029	0.85HisPro: 0.85 ± 0.027	0.438HisGln: 0.438 ± 0.018	0.793HisArg: 0.793 ± 0.023	0.995HisSer: 0.995 ± 0.029	0.915HisThr: 0.915 ± 0.031	1.217HisVal: 1.217 ± 0.032	0.175HisTrp: 0.175 ± 0.015	0.789HisTyr: 0.789 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.683IleAla: 5.683 ± 0.094	1.175IleCys: 1.175 ± 0.032	4.682IleAsp: 4.682 ± 0.071	4.908IleGlu: 4.908 ± 0.076	3.161IlePhe: 3.161 ± 0.061	5.171IleGly: 5.171 ± 0.079	1.347IleHis: 1.347 ± 0.037	5.13IleIle: 5.13 ± 0.085	4.287IleLys: 4.287 ± 0.065	6.61IleLeu: 6.61 ± 0.095	2.136IleMet: 2.136 ± 0.044	3.257IleAsn: 3.257 ± 0.054	3.162IlePro: 3.162 ± 0.054	1.834IleGln: 1.834 ± 0.037	4.066IleArg: 4.066 ± 0.057	5.339IleSer: 5.339 ± 0.082	4.295IleThr: 4.295 ± 0.064	4.962IleVal: 4.962 ± 0.068	0.605IleTrp: 0.605 ± 0.022	2.825IleTyr: 2.825 ± 0.053	0.0IleXaa: 0.0 ± 0.0
Lys	5.217LysAla: 5.217 ± 0.076	0.739LysCys: 0.739 ± 0.028	4.319LysAsp: 4.319 ± 0.064	5.598LysGlu: 5.598 ± 0.079	2.243LysPhe: 2.243 ± 0.045	4.207LysGly: 4.207 ± 0.067	1.001LysHis: 1.001 ± 0.033	4.979LysIle: 4.979 ± 0.068	6.454LysLys: 6.454 ± 0.099	5.197LysLeu: 5.197 ± 0.075	1.988LysMet: 1.988 ± 0.046	3.943LysAsn: 3.943 ± 0.066	2.025LysPro: 2.025 ± 0.05	1.695LysGln: 1.695 ± 0.035	2.908LysArg: 2.908 ± 0.057	3.709LysSer: 3.709 ± 0.057	3.524LysThr: 3.524 ± 0.064	4.292LysVal: 4.292 ± 0.068	0.608LysTrp: 0.608 ± 0.023	3.14LysTyr: 3.14 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	6.039LeuAla: 6.039 ± 0.092	1.267LeuCys: 1.267 ± 0.037	5.153LeuAsp: 5.153 ± 0.062	5.188LeuGlu: 5.188 ± 0.071	3.806LeuPhe: 3.806 ± 0.075	5.328LeuGly: 5.328 ± 0.076	1.482LeuHis: 1.482 ± 0.039	6.037LeuIle: 6.037 ± 0.094	5.962LeuLys: 5.962 ± 0.079	7.337LeuLeu: 7.337 ± 0.108	2.6LeuMet: 2.6 ± 0.06	3.887LeuAsn: 3.887 ± 0.057	3.096LeuPro: 3.096 ± 0.048	2.273LeuGln: 2.273 ± 0.047	3.767LeuArg: 3.767 ± 0.062	6.462LeuSer: 6.462 ± 0.076	4.885LeuThr: 4.885 ± 0.062	5.157LeuVal: 5.157 ± 0.081	0.63LeuTrp: 0.63 ± 0.025	3.172LeuTyr: 3.172 ± 0.061	0.0LeuXaa: 0.0 ± 0.0
Met	2.359MetAla: 2.359 ± 0.065	0.364MetCys: 0.364 ± 0.017	2.001MetAsp: 2.001 ± 0.046	2.126MetGlu: 2.126 ± 0.041	1.127MetPhe: 1.127 ± 0.033	1.99MetGly: 1.99 ± 0.05	0.507MetHis: 0.507 ± 0.019	2.416MetIle: 2.416 ± 0.047	2.451MetLys: 2.451 ± 0.045	2.49MetLeu: 2.49 ± 0.054	0.984MetMet: 0.984 ± 0.043	1.599MetAsn: 1.599 ± 0.039	1.164MetPro: 1.164 ± 0.055	0.919MetGln: 0.919 ± 0.031	1.289MetArg: 1.289 ± 0.036	1.98MetSer: 1.98 ± 0.038	1.821MetThr: 1.821 ± 0.044	1.847MetVal: 1.847 ± 0.038	0.209MetTrp: 0.209 ± 0.015	0.889MetTyr: 0.889 ± 0.029	0.0MetXaa: 0.0 ± 0.0
Asn	3.238AsnAla: 3.238 ± 0.058	0.597AsnCys: 0.597 ± 0.023	2.679AsnAsp: 2.679 ± 0.051	3.17AsnGlu: 3.17 ± 0.057	1.752AsnPhe: 1.752 ± 0.038	3.958AsnGly: 3.958 ± 0.066	0.773AsnHis: 0.773 ± 0.027	3.506AsnIle: 3.506 ± 0.058	2.573AsnLys: 2.573 ± 0.047	3.66AsnLeu: 3.66 ± 0.053	1.401AsnMet: 1.401 ± 0.033	2.06AsnAsn: 2.06 ± 0.05	1.877AsnPro: 1.877 ± 0.043	1.085AsnGln: 1.085 ± 0.031	2.126AsnArg: 2.126 ± 0.045	2.693AsnSer: 2.693 ± 0.058	2.52AsnThr: 2.52 ± 0.052	3.182AsnVal: 3.182 ± 0.057	0.402AsnTrp: 0.402 ± 0.018	1.927AsnTyr: 1.927 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.59ProAla: 2.59 ± 0.061	0.406ProCys: 0.406 ± 0.021	2.77ProAsp: 2.77 ± 0.069	3.533ProGlu: 3.533 ± 0.075	1.538ProPhe: 1.538 ± 0.043	2.262ProGly: 2.262 ± 0.043	0.525ProHis: 0.525 ± 0.021	2.092ProIle: 2.092 ± 0.049	1.847ProLys: 1.847 ± 0.045	2.498ProLeu: 2.498 ± 0.043	0.867ProMet: 0.867 ± 0.031	1.194ProAsn: 1.194 ± 0.033	0.628ProPro: 0.628 ± 0.027	0.805ProGln: 0.805 ± 0.031	1.016ProArg: 1.016 ± 0.034	1.83ProSer: 1.83 ± 0.039	1.478ProThr: 1.478 ± 0.037	2.95ProVal: 2.95 ± 0.053	0.341ProTrp: 0.341 ± 0.018	1.408ProTyr: 1.408 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	1.947GlnAla: 1.947 ± 0.052	0.306GlnCys: 0.306 ± 0.016	1.476GlnAsp: 1.476 ± 0.038	1.971GlnGlu: 1.971 ± 0.047	1.045GlnPhe: 1.045 ± 0.033	1.533GlnGly: 1.533 ± 0.035	0.426GlnHis: 0.426 ± 0.019	2.074GlnIle: 2.074 ± 0.041	2.17GlnLys: 2.17 ± 0.048	2.006GlnLeu: 2.006 ± 0.044	0.952GlnMet: 0.952 ± 0.031	1.448GlnAsn: 1.448 ± 0.039	0.69GlnPro: 0.69 ± 0.026	0.835GlnGln: 0.835 ± 0.032	1.069GlnArg: 1.069 ± 0.03	1.446GlnSer: 1.446 ± 0.036	1.328GlnThr: 1.328 ± 0.038	1.615GlnVal: 1.615 ± 0.04	0.236GlnTrp: 0.236 ± 0.016	1.147GlnTyr: 1.147 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.941ArgAla: 2.941 ± 0.053	0.512ArgCys: 0.512 ± 0.019	2.747ArgAsp: 2.747 ± 0.049	3.785ArgGlu: 3.785 ± 0.07	2.055ArgPhe: 2.055 ± 0.044	2.504ArgGly: 2.504 ± 0.045	0.804ArgHis: 0.804 ± 0.028	3.793ArgIle: 3.793 ± 0.072	3.4ArgLys: 3.4 ± 0.06	3.653ArgLeu: 3.653 ± 0.068	1.456ArgMet: 1.456 ± 0.04	2.271ArgAsn: 2.271 ± 0.047	1.29ArgPro: 1.29 ± 0.033	1.342ArgGln: 1.342 ± 0.036	2.212ArgArg: 2.212 ± 0.056	2.45ArgSer: 2.45 ± 0.05	2.282ArgThr: 2.282 ± 0.047	2.905ArgVal: 2.905 ± 0.054	0.399ArgTrp: 0.399 ± 0.018	1.922ArgTyr: 1.922 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	4.989SerAla: 4.989 ± 0.078	0.723SerCys: 0.723 ± 0.029	4.382SerAsp: 4.382 ± 0.073	4.981SerGlu: 4.981 ± 0.075	3.017SerPhe: 3.017 ± 0.051	5.387SerGly: 5.387 ± 0.07	1.057SerHis: 1.057 ± 0.033	4.433SerIle: 4.433 ± 0.07	3.467SerLys: 3.467 ± 0.061	5.159SerLeu: 5.159 ± 0.07	1.825SerMet: 1.825 ± 0.04	2.391SerAsn: 2.391 ± 0.05	1.777SerPro: 1.777 ± 0.046	1.513SerGln: 1.513 ± 0.04	2.747SerArg: 2.747 ± 0.05	4.026SerSer: 4.026 ± 0.091	3.042SerThr: 3.042 ± 0.061	4.893SerVal: 4.893 ± 0.071	0.58SerTrp: 0.58 ± 0.023	2.499SerTyr: 2.499 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	4.58ThrAla: 4.58 ± 0.073	0.626ThrCys: 0.626 ± 0.025	3.679ThrAsp: 3.679 ± 0.062	4.173ThrGlu: 4.173 ± 0.067	2.204ThrPhe: 2.204 ± 0.045	4.729ThrGly: 4.729 ± 0.069	0.826ThrHis: 0.826 ± 0.027	3.81ThrIle: 3.81 ± 0.06	2.884ThrLys: 2.884 ± 0.054	4.432ThrLeu: 4.432 ± 0.064	1.39ThrMet: 1.39 ± 0.032	1.96ThrAsn: 1.96 ± 0.044	2.076ThrPro: 2.076 ± 0.043	1.214ThrGln: 1.214 ± 0.035	1.942ThrArg: 1.942 ± 0.04	3.052ThrSer: 3.052 ± 0.055	2.771ThrThr: 2.771 ± 0.054	4.632ThrVal: 4.632 ± 0.079	0.477ThrTrp: 0.477 ± 0.024	1.994ThrTyr: 1.994 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.527ValAla: 4.527 ± 0.068	1.045ValCys: 1.045 ± 0.032	4.229ValAsp: 4.229 ± 0.066	4.19ValGlu: 4.19 ± 0.064	3.058ValPhe: 3.058 ± 0.053	4.29ValGly: 4.29 ± 0.075	1.164ValHis: 1.164 ± 0.032	5.644ValIle: 5.644 ± 0.078	4.586ValLys: 4.586 ± 0.077	6.336ValLeu: 6.336 ± 0.092	2.208ValMet: 2.208 ± 0.048	3.177ValAsn: 3.177 ± 0.048	2.646ValPro: 2.646 ± 0.046	1.817ValGln: 1.817 ± 0.039	3.271ValArg: 3.271 ± 0.049	5.121ValSer: 5.121 ± 0.081	4.234ValThr: 4.234 ± 0.075	4.919ValVal: 4.919 ± 0.075	0.62ValTrp: 0.62 ± 0.025	2.747ValTyr: 2.747 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.501TrpAla: 0.501 ± 0.024	0.142TrpCys: 0.142 ± 0.011	0.617TrpAsp: 0.617 ± 0.026	0.544TrpGlu: 0.544 ± 0.022	0.455TrpPhe: 0.455 ± 0.022	0.589TrpGly: 0.589 ± 0.025	0.202TrpHis: 0.202 ± 0.016	0.701TrpIle: 0.701 ± 0.025	0.698TrpLys: 0.698 ± 0.024	0.754TrpLeu: 0.754 ± 0.027	0.298TrpMet: 0.298 ± 0.019	0.599TrpAsn: 0.599 ± 0.024	0.176TrpPro: 0.176 ± 0.013	0.297TrpGln: 0.297 ± 0.016	0.36TrpArg: 0.36 ± 0.016	0.491TrpSer: 0.491 ± 0.023	0.446TrpThr: 0.446 ± 0.023	0.506TrpVal: 0.506 ± 0.024	0.106TrpTrp: 0.106 ± 0.01	0.434TrpTyr: 0.434 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.819TyrAla: 2.819 ± 0.048	0.583TyrCys: 0.583 ± 0.028	2.926TyrAsp: 2.926 ± 0.072	2.89TyrGlu: 2.89 ± 0.053	1.908TyrPhe: 1.908 ± 0.044	2.913TyrGly: 2.913 ± 0.057	0.833TyrHis: 0.833 ± 0.029	2.791TyrIle: 2.791 ± 0.053	2.419TyrLys: 2.419 ± 0.051	3.54TyrLeu: 3.54 ± 0.06	1.125TyrMet: 1.125 ± 0.037	1.827TyrAsn: 1.827 ± 0.046	1.405TyrPro: 1.405 ± 0.039	1.096TyrGln: 1.096 ± 0.036	2.022TyrArg: 2.022 ± 0.046	2.759TyrSer: 2.759 ± 0.052	2.193TyrThr: 2.193 ± 0.054	2.657TyrVal: 2.657 ± 0.051	0.384TyrTrp: 0.384 ± 0.021	1.861TyrTyr: 1.861 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3259 proteins (1122987 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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