Capybara microvirus Cap3_SP_468

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4P8W8P5|A0A4P8W8P5_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_468 OX=2585463 PE=3 SV=1
MM1 pKa = 7.43NKK3 pKa = 9.74LFKK6 pKa = 9.96PDD8 pKa = 3.47EE9 pKa = 4.45VGSYY13 pKa = 9.24PVNVQSDD20 pKa = 3.41FDD22 pKa = 3.37QYY24 pKa = 10.6YY25 pKa = 9.97FEE27 pKa = 4.95NEE29 pKa = 4.1VVKK32 pKa = 10.87KK33 pKa = 9.52FVKK36 pKa = 10.02TGVDD40 pKa = 3.38NEE42 pKa = 4.29GDD44 pKa = 3.53DD45 pKa = 3.65VGYY48 pKa = 9.94IQEE51 pKa = 4.18KK52 pKa = 9.99VIVHH56 pKa = 5.98KK57 pKa = 10.79RR58 pKa = 11.84LIKK61 pKa = 10.75DD62 pKa = 3.4VVAQDD67 pKa = 3.47ADD69 pKa = 4.06SVGVEE74 pKa = 4.6PYY76 pKa = 10.68LKK78 pKa = 10.23MMALSGQSLPDD89 pKa = 3.15VSFTDD94 pKa = 4.36EE95 pKa = 4.68INDD98 pKa = 3.42FTQFPEE104 pKa = 4.25DD105 pKa = 3.18VGDD108 pKa = 4.39AMRR111 pKa = 11.84LGDD114 pKa = 4.59KK115 pKa = 10.99ANALYY120 pKa = 10.69ASLPEE125 pKa = 4.3EE126 pKa = 3.57IRR128 pKa = 11.84RR129 pKa = 11.84NYY131 pKa = 10.02TLDD134 pKa = 2.88QFMQSDD140 pKa = 4.32YY141 pKa = 11.23EE142 pKa = 4.64KK143 pKa = 10.58IVSDD147 pKa = 3.55YY148 pKa = 11.32LALKK152 pKa = 9.63KK153 pKa = 10.78AEE155 pKa = 4.03IEE157 pKa = 3.99AAQKK161 pKa = 10.75GGSEE165 pKa = 4.26NVV167 pKa = 3.13

Molecular weight:
18.85 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4V1FVU0|A0A4V1FVU0_9VIRU Prophage CP4-57 regulatory protein OS=Capybara microvirus Cap3_SP_468 OX=2585463 PE=4 SV=1
MM1 pKa = 7.75TYY3 pKa = 7.39MTKK6 pKa = 9.89QQDD9 pKa = 3.4HH10 pKa = 6.79FIKK13 pKa = 10.03YY14 pKa = 10.33LKK16 pKa = 10.4YY17 pKa = 10.52SLVNVDD23 pKa = 4.09SLEE26 pKa = 4.23NDD28 pKa = 3.32DD29 pKa = 4.79FIYY32 pKa = 10.66RR33 pKa = 11.84IDD35 pKa = 3.58VVVRR39 pKa = 11.84KK40 pKa = 8.12TGISRR45 pKa = 11.84RR46 pKa = 11.84TIYY49 pKa = 10.31RR50 pKa = 11.84LCKK53 pKa = 10.11EE54 pKa = 4.08YY55 pKa = 10.94DD56 pKa = 3.26FPIYY60 pKa = 10.16RR61 pKa = 11.84GCFTVNLARR70 pKa = 11.84FWHH73 pKa = 5.57VFGTNKK79 pKa = 10.25RR80 pKa = 11.84STKK83 pKa = 10.16KK84 pKa = 9.99INLFNTEE91 pKa = 3.68YY92 pKa = 10.57RR93 pKa = 11.84IEE95 pKa = 4.03EE96 pKa = 4.34DD97 pKa = 2.89KK98 pKa = 11.71EE99 pKa = 3.86EE100 pKa = 4.26IYY102 pKa = 10.68RR103 pKa = 11.84IAHH106 pKa = 5.0VNWIGVHH113 pKa = 5.41

Molecular weight:
13.83 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1494

98

535

213.4

24.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.89 ± 0.67

1.339 ± 0.682

6.426 ± 0.695

4.819 ± 0.519

5.154 ± 0.443

6.225 ± 0.624

2.008 ± 0.369

5.957 ± 0.638

7.296 ± 1.029

9.17 ± 0.944

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.268

5.288 ± 0.318

4.15 ± 0.786

4.351 ± 0.545

3.681 ± 0.764

8.099 ± 0.946

5.087 ± 0.74

6.627 ± 0.854

1.071 ± 0.216

5.288 ± 0.632

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski