Pectobacterium phage vB_PatM_CB7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Certrevirus; unclassified Certrevirus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0PBB3|A0A2P0PBB3_9CAUD Uncharacterized protein OS=Pectobacterium phage vB_PatM_CB7 OX=1958916 GN=CB7_131 PE=4 SV=1
MM1 pKa = 7.88IDD3 pKa = 3.68LNLHH7 pKa = 4.62ITRR10 pKa = 11.84TISTGVMFFIPQNEE24 pKa = 4.23DD25 pKa = 2.85QHH27 pKa = 8.08EE28 pKa = 4.4EE29 pKa = 3.91IEE31 pKa = 4.2NTIIAEE37 pKa = 4.21FEE39 pKa = 4.14DD40 pKa = 3.68QDD42 pKa = 3.74FSQVVNLNVDD52 pKa = 3.63NEE54 pKa = 4.63VCCQYY59 pKa = 10.51WLSADD64 pKa = 3.5VDD66 pKa = 4.14DD67 pKa = 4.52EE68 pKa = 4.85AEE70 pKa = 4.1FRR72 pKa = 11.84IACAWVVGKK81 pKa = 8.5MQDD84 pKa = 3.18LGAPLDD90 pKa = 4.14SVSS93 pKa = 3.26

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0PA92|A0A2P0PA92_9CAUD Uncharacterized protein OS=Pectobacterium phage vB_PatM_CB7 OX=1958916 GN=CB7_130 PE=4 SV=1
MM1 pKa = 6.89EE2 pKa = 4.99QIVFYY7 pKa = 10.09SLWALFSVVGVWRR20 pKa = 11.84MVNLMSMSGRR30 pKa = 11.84VGNDD34 pKa = 2.79DD35 pKa = 3.02RR36 pKa = 11.84VMVLFILTPASLLLTPVILVVEE58 pKa = 3.95LLFWVGKK65 pKa = 10.3KK66 pKa = 9.7VDD68 pKa = 3.88NFRR71 pKa = 11.84EE72 pKa = 4.25SPRR75 pKa = 11.84YY76 pKa = 7.54VTWKK80 pKa = 8.9IQRR83 pKa = 11.84QIRR86 pKa = 11.84KK87 pKa = 8.73RR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84GPLVRR95 pKa = 11.84VVEE98 pKa = 3.99HH99 pKa = 6.88LYY101 pKa = 11.01NKK103 pKa = 10.04RR104 pKa = 11.84KK105 pKa = 9.58EE106 pKa = 4.01KK107 pKa = 10.64ARR109 pKa = 11.84EE110 pKa = 3.86EE111 pKa = 4.4GVSEE115 pKa = 4.09II116 pKa = 4.72

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

253

0

253

42417

32

1099

167.7

18.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.068 ± 0.228

1.308 ± 0.104

6.561 ± 0.134

7.014 ± 0.186

4.175 ± 0.133

7.959 ± 0.322

2.084 ± 0.115

5.495 ± 0.109

6.773 ± 0.171

7.676 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.098

4.435 ± 0.115

3.93 ± 0.133

3.35 ± 0.139

5.432 ± 0.173

5.599 ± 0.149

5.576 ± 0.229

7.247 ± 0.145

1.648 ± 0.088

3.767 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski